Direct visualization of human myosin II force generation using DNA origami-based thick filaments View Full Text


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Article Info

DATE

2019-11-27

AUTHORS

Keisuke Fujita, Masashi Ohmachi, Keigo Ikezaki, Toshio Yanagida, Mitsuhiro Iwaki

ABSTRACT

The sarcomere, the minimal mechanical unit of muscle, is composed of myosins, which self-assemble into thick filaments that interact with actin-based thin filaments in a highly-structured lattice. This complex imposes a geometric restriction on myosin in force generation. However, how single myosins generate force within the restriction remains elusive and conventional synthetic filaments do not recapitulate the symmetric bipolar filaments in sarcomeres. Here we engineered thick filaments using DNA origami that incorporate human muscle myosin to directly visualize the motion of the heads during force generation in a restricted space. We found that when the head diffuses, it weakly interacts with actin filaments and then strongly binds preferentially to the forward region as a Brownian ratchet. Upon strong binding, the two-step lever-arm swing dominantly halts at the first step and occasionally reverses direction. Our results illustrate the usefulness of our DNA origami-based assay system to dissect the mechanistic details of motor proteins. More... »

PAGES

437

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s42003-019-0683-0

    DOI

    http://dx.doi.org/10.1038/s42003-019-0683-0

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/31799438


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    47 DNA origami
    48 actin
    49 actin filaments
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    56 direction
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    63 generation
    64 geometric restrictions
    65 halt
    66 head
    67 human muscle myosin
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    70 mechanical units
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    72 motion
    73 motor proteins
    74 muscle
    75 muscle myosin
    76 myosin
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    81 region
    82 restriction
    83 results
    84 sarcomeres
    85 space
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