Background-deflection Brillouin microscopy reveals altered biomechanics of intracellular stress granules by ALS protein FUS View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-12

AUTHORS

Giuseppe Antonacci, Valeria de Turris, Alessandro Rosa, Giancarlo Ruocco

ABSTRACT

Altered cellular biomechanics have been implicated as key photogenic triggers in age-related diseases. An aberrant liquid-to-solid phase transition, observed in in vitro reconstituted droplets of FUS protein, has been recently proposed as a possible pathogenic mechanism for amyotrophic lateral sclerosis (ALS). Whether such transition occurs in cell environments is currently unknown as a consequence of the limited measuring capability of the existing techniques, which are invasive or lack of subcellular resolution. Here we developed a non-contact and label-free imaging method, named background-deflection Brillouin microscopy, to investigate the three-dimensional intracellular biomechanics at a sub-micron resolution. Our method exploits diffraction to achieve an unprecedented 10,000-fold enhancement in the spectral contrast of single-stage spectrometers, enabling, to the best of our knowledge, the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells. Our findings provide fundamental insights on the critical aggregation step underlying the neurodegenerative ALS disease. More... »

PAGES

139

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s42003-018-0148-x

DOI

http://dx.doi.org/10.1038/s42003-018-0148-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1106681241

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30272018


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Italian Institute of Technology", 
          "id": "https://www.grid.ac/institutes/grid.25786.3e", 
          "name": [
            "1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Antonacci", 
        "givenName": "Giuseppe", 
        "id": "sg:person.014124237551.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014124237551.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Italian Institute of Technology", 
          "id": "https://www.grid.ac/institutes/grid.25786.3e", 
          "name": [
            "1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Turris", 
        "givenName": "Valeria", 
        "id": "sg:person.0717015614.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717015614.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapienza University of Rome", 
          "id": "https://www.grid.ac/institutes/grid.7841.a", 
          "name": [
            "1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.", 
            "2Department of Biology and Biotechnology Charles Darwin, University of Rome\"Sapienza\", Rome, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rosa", 
        "givenName": "Alessandro", 
        "id": "sg:person.0734636771.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734636771.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Sapienza University of Rome", 
          "id": "https://www.grid.ac/institutes/grid.7841.a", 
          "name": [
            "1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.", 
            "3Department of Physics, University of Rome \"Sapienza\", Rome, Italy."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ruocco", 
        "givenName": "Giancarlo", 
        "id": "sg:person.0722713612.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722713612.47"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1117/1.jbo.19.7.075007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001987795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0596-601", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007457663", 
          "https://doi.org/10.1038/nm0596-601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1258/cvd.2012.012016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007942360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1258/cvd.2012.012016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007942360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuroimage.2009.06.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013354627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth1078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014312710", 
          "https://doi.org/10.1038/nmeth1078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2016.10.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015044250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep37217", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016668610", 
          "https://doi.org/10.1038/srep37217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmps.2003.09.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018565966"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.actamat.2007.04.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018569973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1104651108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018907898"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1361-8415(00)00039-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020723627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3an02168h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021394812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biophys.050708.133724", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025752574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2011.08.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026229022"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200502088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027458451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200502088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027458451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/scisignal.aaf6326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028617510"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuron.2015.10.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029736062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rsif.2015.0843", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033423802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/oe.19.010913", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034540499"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/284489a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035528870", 
          "https://doi.org/10.1038/284489a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s003390051204", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037662441", 
          "https://doi.org/10.1007/s003390051204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/09500340110088551", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038748878"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1750-1326-7-56", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038751873", 
          "https://doi.org/10.1186/1750-1326-7-56"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nphoton.2007.250", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040168083", 
          "https://doi.org/10.1038/nphoton.2007.250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1167/iovs.11-8281", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040926169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jbio.201400047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042698367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.3616", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043162217", 
          "https://doi.org/10.1038/nmeth.3616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2818.2005.01497.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043379074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2818.2005.01497.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043379074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05524", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049196964", 
          "https://doi.org/10.1038/nature05524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05524", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049196964", 
          "https://doi.org/10.1038/nature05524"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.4948353", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049392392"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2015.07.047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049395074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la034136x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056145466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la034136x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056145466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.1999857", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057835066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.4836477", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058086821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.4927400", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058094355"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/0022-3735/20/6/017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058973694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevapplied.6.054020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060517977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevapplied.6.054020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060517977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7973655", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062650779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/016173469101300201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063748863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/016173469101300201", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063748863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/oe.22.005410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1065206731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/ol.21.000366", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1065216572"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1379/1466-1268(2002)007<0213:vstroe>2.0.co;2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067173793", 
          "https://doi.org/10.1379/1466-1268(2002)007<0213:vstroe>2.0.co;2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082569795", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1142/s1793545817420019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084227620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1364/ol.42.001432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084269415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.15252/embj.201695957", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084513161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nphoton.2017.6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084537717", 
          "https://doi.org/10.1038/nphoton.2017.6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevx.7.031015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090861002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevx.7.031015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090861002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aaf4382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091886491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15548627.2017.1384889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092091593"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-12", 
    "datePublishedReg": "2018-12-01", 
    "description": "Altered cellular biomechanics have been implicated as key photogenic triggers in age-related diseases. An aberrant liquid-to-solid phase transition, observed in in vitro reconstituted droplets of FUS protein, has been recently proposed as a possible pathogenic mechanism for amyotrophic lateral sclerosis (ALS). Whether such transition occurs in cell environments is currently unknown as a consequence of the limited measuring capability of the existing techniques, which are invasive or lack of subcellular resolution. Here we developed a non-contact and label-free imaging method, named background-deflection Brillouin microscopy, to investigate the three-dimensional intracellular biomechanics at a sub-micron resolution. Our method exploits diffraction to achieve an unprecedented 10,000-fold enhancement in the spectral contrast of single-stage spectrometers, enabling, to the best of our knowledge, the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells. Our findings provide fundamental insights on the critical aggregation step underlying the neurodegenerative ALS disease.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s42003-018-0148-x", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1300829", 
        "issn": [
          "2399-3642"
        ], 
        "name": "Communications Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "1"
      }
    ], 
    "name": "Background-deflection Brillouin microscopy reveals altered biomechanics of intracellular stress granules by ALS protein FUS", 
    "pagination": "139", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "63d484dadab16ea38dfecf5564f615229f0a4796266e8bc233a340b5849d8876"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30272018"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101719179"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s42003-018-0148-x"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1106681241"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s42003-018-0148-x", 
      "https://app.dimensions.ai/details/publication/pub.1106681241"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T02:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000548.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s42003-018-0148-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s42003-018-0148-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s42003-018-0148-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s42003-018-0148-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s42003-018-0148-x'


 

This table displays all metadata directly associated to this object as RDF triples.

254 TRIPLES      21 PREDICATES      79 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s42003-018-0148-x schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N10fcd21b524e41c3808a9a13edb533b4
4 schema:citation sg:pub.10.1007/s003390051204
5 sg:pub.10.1038/284489a0
6 sg:pub.10.1038/nature05524
7 sg:pub.10.1038/nm0596-601
8 sg:pub.10.1038/nmeth.3616
9 sg:pub.10.1038/nmeth1078
10 sg:pub.10.1038/nphoton.2007.250
11 sg:pub.10.1038/nphoton.2017.6
12 sg:pub.10.1038/srep37217
13 sg:pub.10.1186/1750-1326-7-56
14 sg:pub.10.1379/1466-1268(2002)007<0213:vstroe>2.0.co;2
15 https://app.dimensions.ai/details/publication/pub.1082569795
16 https://doi.org/10.1002/jbio.201400047
17 https://doi.org/10.1016/j.actamat.2007.04.022
18 https://doi.org/10.1016/j.bpj.2011.08.008
19 https://doi.org/10.1016/j.cell.2015.07.047
20 https://doi.org/10.1016/j.jmps.2003.09.019
21 https://doi.org/10.1016/j.neuroimage.2009.06.018
22 https://doi.org/10.1016/j.neuron.2015.10.030
23 https://doi.org/10.1016/j.sbi.2016.10.015
24 https://doi.org/10.1016/s1361-8415(00)00039-6
25 https://doi.org/10.1021/la034136x
26 https://doi.org/10.1039/c3an02168h
27 https://doi.org/10.1063/1.1999857
28 https://doi.org/10.1063/1.4836477
29 https://doi.org/10.1063/1.4927400
30 https://doi.org/10.1063/1.4948353
31 https://doi.org/10.1073/pnas.1104651108
32 https://doi.org/10.1080/09500340110088551
33 https://doi.org/10.1080/15548627.2017.1384889
34 https://doi.org/10.1083/jcb.200502088
35 https://doi.org/10.1088/0022-3735/20/6/017
36 https://doi.org/10.1098/rsif.2015.0843
37 https://doi.org/10.1103/physrevapplied.6.054020
38 https://doi.org/10.1103/physrevx.7.031015
39 https://doi.org/10.1111/j.1365-2818.2005.01497.x
40 https://doi.org/10.1117/1.jbo.19.7.075007
41 https://doi.org/10.1126/science.7973655
42 https://doi.org/10.1126/science.aaf4382
43 https://doi.org/10.1126/scisignal.aaf6326
44 https://doi.org/10.1142/s1793545817420019
45 https://doi.org/10.1146/annurev.biophys.050708.133724
46 https://doi.org/10.1167/iovs.11-8281
47 https://doi.org/10.1177/016173469101300201
48 https://doi.org/10.1258/cvd.2012.012016
49 https://doi.org/10.1364/oe.19.010913
50 https://doi.org/10.1364/oe.22.005410
51 https://doi.org/10.1364/ol.21.000366
52 https://doi.org/10.1364/ol.42.001432
53 https://doi.org/10.15252/embj.201695957
54 schema:datePublished 2018-12
55 schema:datePublishedReg 2018-12-01
56 schema:description Altered cellular biomechanics have been implicated as key photogenic triggers in age-related diseases. An aberrant liquid-to-solid phase transition, observed in in vitro reconstituted droplets of FUS protein, has been recently proposed as a possible pathogenic mechanism for amyotrophic lateral sclerosis (ALS). Whether such transition occurs in cell environments is currently unknown as a consequence of the limited measuring capability of the existing techniques, which are invasive or lack of subcellular resolution. Here we developed a non-contact and label-free imaging method, named background-deflection Brillouin microscopy, to investigate the three-dimensional intracellular biomechanics at a sub-micron resolution. Our method exploits diffraction to achieve an unprecedented 10,000-fold enhancement in the spectral contrast of single-stage spectrometers, enabling, to the best of our knowledge, the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells. Our findings provide fundamental insights on the critical aggregation step underlying the neurodegenerative ALS disease.
57 schema:genre research_article
58 schema:inLanguage en
59 schema:isAccessibleForFree false
60 schema:isPartOf N190bcb8fd96045b79ca6aa2a278144ad
61 N3004da4343e647ac8160aa42e04f9468
62 sg:journal.1300829
63 schema:name Background-deflection Brillouin microscopy reveals altered biomechanics of intracellular stress granules by ALS protein FUS
64 schema:pagination 139
65 schema:productId N083144ad7ad144d481de87eb59a198a4
66 N3b07214a640c456bab64ff7f3ff92e73
67 N4304026bc17c4ebc8c949c6771efc99f
68 Nc404e37eae0b419fb5b01724d8a902ae
69 Nce65a1ec54ca4a2eb7124cd0650a9146
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1106681241
71 https://doi.org/10.1038/s42003-018-0148-x
72 schema:sdDatePublished 2019-04-11T02:18
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher Nb97d0e619a3e47a9992c5f961c96111a
75 schema:url https://www.nature.com/articles/s42003-018-0148-x
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N083144ad7ad144d481de87eb59a198a4 schema:name dimensions_id
80 schema:value pub.1106681241
81 rdf:type schema:PropertyValue
82 N10fcd21b524e41c3808a9a13edb533b4 rdf:first sg:person.014124237551.00
83 rdf:rest Naecd11e7671949619eaed00de56d7b33
84 N123be842c24d49e49a68eddc749f0a9d rdf:first sg:person.0722713612.47
85 rdf:rest rdf:nil
86 N190bcb8fd96045b79ca6aa2a278144ad schema:issueNumber 1
87 rdf:type schema:PublicationIssue
88 N20a7fd14d0e7439f9d059fd15e7d5d36 rdf:first sg:person.0734636771.37
89 rdf:rest N123be842c24d49e49a68eddc749f0a9d
90 N3004da4343e647ac8160aa42e04f9468 schema:volumeNumber 1
91 rdf:type schema:PublicationVolume
92 N3b07214a640c456bab64ff7f3ff92e73 schema:name pubmed_id
93 schema:value 30272018
94 rdf:type schema:PropertyValue
95 N4304026bc17c4ebc8c949c6771efc99f schema:name nlm_unique_id
96 schema:value 101719179
97 rdf:type schema:PropertyValue
98 Naecd11e7671949619eaed00de56d7b33 rdf:first sg:person.0717015614.16
99 rdf:rest N20a7fd14d0e7439f9d059fd15e7d5d36
100 Nb97d0e619a3e47a9992c5f961c96111a schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 Nc404e37eae0b419fb5b01724d8a902ae schema:name readcube_id
103 schema:value 63d484dadab16ea38dfecf5564f615229f0a4796266e8bc233a340b5849d8876
104 rdf:type schema:PropertyValue
105 Nce65a1ec54ca4a2eb7124cd0650a9146 schema:name doi
106 schema:value 10.1038/s42003-018-0148-x
107 rdf:type schema:PropertyValue
108 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
109 schema:name Biological Sciences
110 rdf:type schema:DefinedTerm
111 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
112 schema:name Biochemistry and Cell Biology
113 rdf:type schema:DefinedTerm
114 sg:journal.1300829 schema:issn 2399-3642
115 schema:name Communications Biology
116 rdf:type schema:Periodical
117 sg:person.014124237551.00 schema:affiliation https://www.grid.ac/institutes/grid.25786.3e
118 schema:familyName Antonacci
119 schema:givenName Giuseppe
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014124237551.00
121 rdf:type schema:Person
122 sg:person.0717015614.16 schema:affiliation https://www.grid.ac/institutes/grid.25786.3e
123 schema:familyName de Turris
124 schema:givenName Valeria
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717015614.16
126 rdf:type schema:Person
127 sg:person.0722713612.47 schema:affiliation https://www.grid.ac/institutes/grid.7841.a
128 schema:familyName Ruocco
129 schema:givenName Giancarlo
130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0722713612.47
131 rdf:type schema:Person
132 sg:person.0734636771.37 schema:affiliation https://www.grid.ac/institutes/grid.7841.a
133 schema:familyName Rosa
134 schema:givenName Alessandro
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734636771.37
136 rdf:type schema:Person
137 sg:pub.10.1007/s003390051204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037662441
138 https://doi.org/10.1007/s003390051204
139 rdf:type schema:CreativeWork
140 sg:pub.10.1038/284489a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035528870
141 https://doi.org/10.1038/284489a0
142 rdf:type schema:CreativeWork
143 sg:pub.10.1038/nature05524 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049196964
144 https://doi.org/10.1038/nature05524
145 rdf:type schema:CreativeWork
146 sg:pub.10.1038/nm0596-601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007457663
147 https://doi.org/10.1038/nm0596-601
148 rdf:type schema:CreativeWork
149 sg:pub.10.1038/nmeth.3616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043162217
150 https://doi.org/10.1038/nmeth.3616
151 rdf:type schema:CreativeWork
152 sg:pub.10.1038/nmeth1078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014312710
153 https://doi.org/10.1038/nmeth1078
154 rdf:type schema:CreativeWork
155 sg:pub.10.1038/nphoton.2007.250 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040168083
156 https://doi.org/10.1038/nphoton.2007.250
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/nphoton.2017.6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084537717
159 https://doi.org/10.1038/nphoton.2017.6
160 rdf:type schema:CreativeWork
161 sg:pub.10.1038/srep37217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016668610
162 https://doi.org/10.1038/srep37217
163 rdf:type schema:CreativeWork
164 sg:pub.10.1186/1750-1326-7-56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038751873
165 https://doi.org/10.1186/1750-1326-7-56
166 rdf:type schema:CreativeWork
167 sg:pub.10.1379/1466-1268(2002)007<0213:vstroe>2.0.co;2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067173793
168 https://doi.org/10.1379/1466-1268(2002)007<0213:vstroe>2.0.co;2
169 rdf:type schema:CreativeWork
170 https://app.dimensions.ai/details/publication/pub.1082569795 schema:CreativeWork
171 https://doi.org/10.1002/jbio.201400047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042698367
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1016/j.actamat.2007.04.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018569973
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/j.bpj.2011.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026229022
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/j.cell.2015.07.047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049395074
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/j.jmps.2003.09.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018565966
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/j.neuroimage.2009.06.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013354627
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/j.neuron.2015.10.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029736062
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.sbi.2016.10.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015044250
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/s1361-8415(00)00039-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020723627
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1021/la034136x schema:sameAs https://app.dimensions.ai/details/publication/pub.1056145466
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1039/c3an02168h schema:sameAs https://app.dimensions.ai/details/publication/pub.1021394812
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1063/1.1999857 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057835066
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1063/1.4836477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058086821
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1063/1.4927400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058094355
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1063/1.4948353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049392392
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1073/pnas.1104651108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018907898
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1080/09500340110088551 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038748878
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1080/15548627.2017.1384889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092091593
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1083/jcb.200502088 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027458451
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1088/0022-3735/20/6/017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058973694
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1098/rsif.2015.0843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033423802
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1103/physrevapplied.6.054020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060517977
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1103/physrevx.7.031015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090861002
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1111/j.1365-2818.2005.01497.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043379074
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1117/1.jbo.19.7.075007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001987795
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1126/science.7973655 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062650779
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1126/science.aaf4382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091886491
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1126/scisignal.aaf6326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028617510
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1142/s1793545817420019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084227620
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1146/annurev.biophys.050708.133724 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025752574
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1167/iovs.11-8281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040926169
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1177/016173469101300201 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063748863
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1258/cvd.2012.012016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007942360
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1364/oe.19.010913 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034540499
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1364/oe.22.005410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1065206731
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1364/ol.21.000366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1065216572
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1364/ol.42.001432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084269415
244 rdf:type schema:CreativeWork
245 https://doi.org/10.15252/embj.201695957 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084513161
246 rdf:type schema:CreativeWork
247 https://www.grid.ac/institutes/grid.25786.3e schema:alternateName Italian Institute of Technology
248 schema:name 1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.
249 rdf:type schema:Organization
250 https://www.grid.ac/institutes/grid.7841.a schema:alternateName Sapienza University of Rome
251 schema:name 1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.
252 2Department of Biology and Biotechnology Charles Darwin, University of Rome"Sapienza", Rome, Italy.
253 3Department of Physics, University of Rome "Sapienza", Rome, Italy.
254 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...