Smooth muscle protein 22α-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-07-07

AUTHORS

Shinya Ikeda, Sachiko Sugioka, Takeshi Kimura, Noboru Ashida

ABSTRACT

The Cre-loxP system has been widely used for cell- or organ-specific gene manipulation, but it is important to precisely understand what kind of cells the recombination takes place in. Smooth muscle 22α (SM22α)-Cre mice have been utilized to alter genes in vascular smooth muscle cells (VSMCs), activated fibroblasts or cardiomyocytes (CMs). Moreover, previous reports indicated that SM22α-Cre is expressed in adipocytes, platelets or myeloid cells. However, there have been no report of whether SM22α-Cre recombination takes place in nonCMs in hearts. Thus, we used the double-fluorescent Cre reporter mouse in which GFP is expressed when recombination occurs. Immunofluorescence analysis demonstrated that recombination occurred in resting cardiac fibroblasts (CFs) or macrophages, as well as VSMCs and CMs. Flow cytometry showed that some CFs, resident macrophages, neutrophils, T cells, and B cells were positive for GFP. These results prompted us to analyze bone marrow cells, and we observed GFP-positive hematopoietic precursor cells (HPCs). Taken together, these results indicated that SM22α-Cre-mediated recombination occurs in resting CFs and hematopoietic cell lineages, including HPCs, which is a cautionary point when using SM22α-Cre mice. More... »

PAGES

11564

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-022-15957-2

DOI

http://dx.doi.org/10.1038/s41598-022-15957-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1149303724

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/35798848


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fatigue Syndrome, Chronic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibroblasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Integrases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Transgenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microfilament Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscle, Smooth, Vascular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myocytes, Cardiac", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myocytes, Smooth Muscle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombination, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ikeda", 
        "givenName": "Shinya", 
        "id": "sg:person.013321664604.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013321664604.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sugioka", 
        "givenName": "Sachiko", 
        "id": "sg:person.012524304204.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012524304204.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hirakata Kohsai Hospital, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan", 
            "Hirakata Kohsai Hospital, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kimura", 
        "givenName": "Takeshi", 
        "id": "sg:person.012173163322.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012173163322.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan", 
          "id": "http://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ashida", 
        "givenName": "Noboru", 
        "id": "sg:person.015723477241.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015723477241.95"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/sdata.2017.105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091375015", 
          "https://doi.org/10.1038/sdata.2017.105"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41569-022-00701-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1147655472", 
          "https://doi.org/10.1038/s41569-022-00701-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-018-21948-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101130659", 
          "https://doi.org/10.1038/s41598-018-21948-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms12260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020999325", 
          "https://doi.org/10.1038/ncomms12260"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2022-07-07", 
    "datePublishedReg": "2022-07-07", 
    "description": "The Cre-loxP system has been widely used for cell- or organ-specific gene manipulation, but it is important to precisely understand what kind of cells the recombination takes place in. Smooth muscle 22\u03b1 (SM22\u03b1)-Cre mice have been utilized to alter genes in vascular smooth muscle cells (VSMCs), activated fibroblasts or cardiomyocytes (CMs). Moreover, previous reports indicated that SM22\u03b1-Cre is expressed in adipocytes, platelets or myeloid cells. However, there have been no report of whether SM22\u03b1-Cre recombination takes place in nonCMs in hearts. Thus, we used the double-fluorescent Cre reporter mouse in which GFP is expressed when recombination occurs. Immunofluorescence analysis demonstrated that recombination occurred in resting cardiac fibroblasts (CFs) or macrophages, as well as VSMCs and CMs. Flow cytometry showed that some CFs, resident macrophages, neutrophils, T cells, and B cells were positive for GFP. These results prompted us to analyze bone marrow cells, and we observed GFP-positive hematopoietic precursor cells (HPCs). Taken together, these results indicated that SM22\u03b1-Cre-mediated recombination occurs in resting CFs and hematopoietic cell lineages, including HPCs, which is a cautionary point when using SM22\u03b1-Cre mice.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/s41598-022-15957-2", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5906998", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7534898", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.9683184", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.6124014", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "keywords": [
      "vascular smooth muscle cells", 
      "hematopoietic precursor cells", 
      "SM22\u03b1-Cre", 
      "hematopoietic cell lineages", 
      "cardiac fibroblasts", 
      "SM22\u03b1-Cre mice", 
      "smooth muscle 22\u03b1", 
      "Cre-loxP system", 
      "gene manipulation", 
      "cell lineages", 
      "Cre reporter mice", 
      "hematopoietic precursors", 
      "smooth muscle cells", 
      "bone marrow cells", 
      "precursor cells", 
      "immunofluorescence analysis", 
      "kinds of cells", 
      "GFP", 
      "muscle cells", 
      "recombination", 
      "reporter mice", 
      "myeloid cells", 
      "resident macrophages", 
      "marrow cells", 
      "fibroblasts", 
      "cells", 
      "Cre mice", 
      "flow cytometry", 
      "cardiomyocytes", 
      "lineages", 
      "genes", 
      "B cells", 
      "macrophages", 
      "mice", 
      "adipocytes", 
      "T cells", 
      "previous reports", 
      "cytometry", 
      "manipulation", 
      "precursors", 
      "cautionary points", 
      "platelets", 
      "neutrophils", 
      "analysis", 
      "results", 
      "heart", 
      "report", 
      "place", 
      "system", 
      "point", 
      "kind"
    ], 
    "name": "Smooth muscle protein 22\u03b1-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors", 
    "pagination": "11564", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1149303724"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-022-15957-2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "35798848"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-022-15957-2", 
      "https://app.dimensions.ai/details/publication/pub.1149303724"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T16:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_928.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/s41598-022-15957-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-022-15957-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-022-15957-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-022-15957-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-022-15957-2'


 

This table displays all metadata directly associated to this object as RDF triples.

208 TRIPLES      21 PREDICATES      92 URIs      80 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-022-15957-2 schema:about N124504f2da3a4592a82c3de8875c7afe
2 N1664414632f54e9bb11097f4af58eee2
3 N254982669a7c4b5cbd88f7f48fbe2c77
4 N50b7a8e4e1b549f3b9fdf54d2639307a
5 N5f59fa558db846fe8589a4d204997c9a
6 N781ca222daf04a568efe4aff6ec5e3e1
7 Na1cc31c00e124898a27de7cc475d0b43
8 Nac5015ef1c4c415282322e27b9fa5fd0
9 Nd3c4c7244888455bbdd61b429e543d3f
10 Nd7fcbcdf91384192a2b2af3eb9e2c324
11 Nf1693ab28fd043d19c6a47139d02d23e
12 Nf4511e49cb794a40825ab18885c32662
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N344e7a3823e8491e892a0008124d1fa7
16 schema:citation sg:pub.10.1038/ncomms12260
17 sg:pub.10.1038/s41569-022-00701-0
18 sg:pub.10.1038/s41598-018-21948-z
19 sg:pub.10.1038/sdata.2017.105
20 schema:datePublished 2022-07-07
21 schema:datePublishedReg 2022-07-07
22 schema:description The Cre-loxP system has been widely used for cell- or organ-specific gene manipulation, but it is important to precisely understand what kind of cells the recombination takes place in. Smooth muscle 22α (SM22α)-Cre mice have been utilized to alter genes in vascular smooth muscle cells (VSMCs), activated fibroblasts or cardiomyocytes (CMs). Moreover, previous reports indicated that SM22α-Cre is expressed in adipocytes, platelets or myeloid cells. However, there have been no report of whether SM22α-Cre recombination takes place in nonCMs in hearts. Thus, we used the double-fluorescent Cre reporter mouse in which GFP is expressed when recombination occurs. Immunofluorescence analysis demonstrated that recombination occurred in resting cardiac fibroblasts (CFs) or macrophages, as well as VSMCs and CMs. Flow cytometry showed that some CFs, resident macrophages, neutrophils, T cells, and B cells were positive for GFP. These results prompted us to analyze bone marrow cells, and we observed GFP-positive hematopoietic precursor cells (HPCs). Taken together, these results indicated that SM22α-Cre-mediated recombination occurs in resting CFs and hematopoietic cell lineages, including HPCs, which is a cautionary point when using SM22α-Cre mice.
23 schema:genre article
24 schema:isAccessibleForFree true
25 schema:isPartOf N47b1b53561fa48d0bf3550b7ca6f93df
26 Nacf7aaf4879748b5a1c60b2994fcc745
27 sg:journal.1045337
28 schema:keywords B cells
29 Cre mice
30 Cre reporter mice
31 Cre-loxP system
32 GFP
33 SM22α-Cre
34 SM22α-Cre mice
35 T cells
36 adipocytes
37 analysis
38 bone marrow cells
39 cardiac fibroblasts
40 cardiomyocytes
41 cautionary points
42 cell lineages
43 cells
44 cytometry
45 fibroblasts
46 flow cytometry
47 gene manipulation
48 genes
49 heart
50 hematopoietic cell lineages
51 hematopoietic precursor cells
52 hematopoietic precursors
53 immunofluorescence analysis
54 kind
55 kinds of cells
56 lineages
57 macrophages
58 manipulation
59 marrow cells
60 mice
61 muscle cells
62 myeloid cells
63 neutrophils
64 place
65 platelets
66 point
67 precursor cells
68 precursors
69 previous reports
70 recombination
71 report
72 reporter mice
73 resident macrophages
74 results
75 smooth muscle 22α
76 smooth muscle cells
77 system
78 vascular smooth muscle cells
79 schema:name Smooth muscle protein 22α-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors
80 schema:pagination 11564
81 schema:productId N28fc16dbd2a5409b8e19f2fe1cbd4cac
82 N2ec0fe349741441c8ce2d02664737a80
83 N750344bfb67044079a040468b4ad14b5
84 schema:sameAs https://app.dimensions.ai/details/publication/pub.1149303724
85 https://doi.org/10.1038/s41598-022-15957-2
86 schema:sdDatePublished 2022-09-02T16:08
87 schema:sdLicense https://scigraph.springernature.com/explorer/license/
88 schema:sdPublisher Nd209e70b7dfc462b8d4cf4f5e1cb8f2a
89 schema:url https://doi.org/10.1038/s41598-022-15957-2
90 sgo:license sg:explorer/license/
91 sgo:sdDataset articles
92 rdf:type schema:ScholarlyArticle
93 N124504f2da3a4592a82c3de8875c7afe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Animals
95 rdf:type schema:DefinedTerm
96 N1664414632f54e9bb11097f4af58eee2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Fatigue Syndrome, Chronic
98 rdf:type schema:DefinedTerm
99 N254982669a7c4b5cbd88f7f48fbe2c77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Mice
101 rdf:type schema:DefinedTerm
102 N28fc16dbd2a5409b8e19f2fe1cbd4cac schema:name doi
103 schema:value 10.1038/s41598-022-15957-2
104 rdf:type schema:PropertyValue
105 N2ec0fe349741441c8ce2d02664737a80 schema:name dimensions_id
106 schema:value pub.1149303724
107 rdf:type schema:PropertyValue
108 N344e7a3823e8491e892a0008124d1fa7 rdf:first sg:person.013321664604.35
109 rdf:rest N658db26edfcd4b9a8da6037071830ed4
110 N47b1b53561fa48d0bf3550b7ca6f93df schema:volumeNumber 12
111 rdf:type schema:PublicationVolume
112 N50b7a8e4e1b549f3b9fdf54d2639307a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Muscle, Smooth, Vascular
114 rdf:type schema:DefinedTerm
115 N5f59fa558db846fe8589a4d204997c9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Muscle Proteins
117 rdf:type schema:DefinedTerm
118 N658db26edfcd4b9a8da6037071830ed4 rdf:first sg:person.012524304204.53
119 rdf:rest Ncba2129b8adb4750a418a42e2eded13c
120 N750344bfb67044079a040468b4ad14b5 schema:name pubmed_id
121 schema:value 35798848
122 rdf:type schema:PropertyValue
123 N781ca222daf04a568efe4aff6ec5e3e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Fibroblasts
125 rdf:type schema:DefinedTerm
126 Na1cc31c00e124898a27de7cc475d0b43 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Mice, Transgenic
128 rdf:type schema:DefinedTerm
129 Nac5015ef1c4c415282322e27b9fa5fd0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Myocytes, Smooth Muscle
131 rdf:type schema:DefinedTerm
132 Nacf7aaf4879748b5a1c60b2994fcc745 schema:issueNumber 1
133 rdf:type schema:PublicationIssue
134 Ncb4819fc04d1446b84f8ca9665217030 rdf:first sg:person.015723477241.95
135 rdf:rest rdf:nil
136 Ncba2129b8adb4750a418a42e2eded13c rdf:first sg:person.012173163322.36
137 rdf:rest Ncb4819fc04d1446b84f8ca9665217030
138 Nd209e70b7dfc462b8d4cf4f5e1cb8f2a schema:name Springer Nature - SN SciGraph project
139 rdf:type schema:Organization
140 Nd3c4c7244888455bbdd61b429e543d3f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Microfilament Proteins
142 rdf:type schema:DefinedTerm
143 Nd7fcbcdf91384192a2b2af3eb9e2c324 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Recombination, Genetic
145 rdf:type schema:DefinedTerm
146 Nf1693ab28fd043d19c6a47139d02d23e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Integrases
148 rdf:type schema:DefinedTerm
149 Nf4511e49cb794a40825ab18885c32662 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
150 schema:name Myocytes, Cardiac
151 rdf:type schema:DefinedTerm
152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biological Sciences
154 rdf:type schema:DefinedTerm
155 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
156 schema:name Biochemistry and Cell Biology
157 rdf:type schema:DefinedTerm
158 sg:grant.5906998 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-022-15957-2
159 rdf:type schema:MonetaryGrant
160 sg:grant.6124014 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-022-15957-2
161 rdf:type schema:MonetaryGrant
162 sg:grant.7534898 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-022-15957-2
163 rdf:type schema:MonetaryGrant
164 sg:grant.9683184 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-022-15957-2
165 rdf:type schema:MonetaryGrant
166 sg:journal.1045337 schema:issn 2045-2322
167 schema:name Scientific Reports
168 schema:publisher Springer Nature
169 rdf:type schema:Periodical
170 sg:person.012173163322.36 schema:affiliation grid-institutes:None
171 schema:familyName Kimura
172 schema:givenName Takeshi
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012173163322.36
174 rdf:type schema:Person
175 sg:person.012524304204.53 schema:affiliation grid-institutes:grid.258799.8
176 schema:familyName Sugioka
177 schema:givenName Sachiko
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012524304204.53
179 rdf:type schema:Person
180 sg:person.013321664604.35 schema:affiliation grid-institutes:grid.258799.8
181 schema:familyName Ikeda
182 schema:givenName Shinya
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013321664604.35
184 rdf:type schema:Person
185 sg:person.015723477241.95 schema:affiliation grid-institutes:grid.258799.8
186 schema:familyName Ashida
187 schema:givenName Noboru
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015723477241.95
189 rdf:type schema:Person
190 sg:pub.10.1038/ncomms12260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020999325
191 https://doi.org/10.1038/ncomms12260
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/s41569-022-00701-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1147655472
194 https://doi.org/10.1038/s41569-022-00701-0
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/s41598-018-21948-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1101130659
197 https://doi.org/10.1038/s41598-018-21948-z
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/sdata.2017.105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091375015
200 https://doi.org/10.1038/sdata.2017.105
201 rdf:type schema:CreativeWork
202 grid-institutes:None schema:alternateName Hirakata Kohsai Hospital, Osaka, Japan
203 schema:name Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan
204 Hirakata Kohsai Hospital, Osaka, Japan
205 rdf:type schema:Organization
206 grid-institutes:grid.258799.8 schema:alternateName Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan
207 schema:name Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507, Kyoto, Japan
208 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...