Comparative analysis of mesenchymal stem cells cultivated in serum free media View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2022-05-21

AUTHORS

Joo Youn Lee, Min Hee Kang, Ji Eun Jang, Jeong Eon Lee, Yuyeong Yang, Ji Yong Choi, Hong Seok Kang, Uiil Lee, Ji Woong Choung, Hyeryeon Jung, Young-Chan Yoon, Kyung Hee Jung, Soon–Sun Hong, Eugene C. Yi, Sang Gyu Park

ABSTRACT

Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity. More... »

PAGES

8620

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-022-12467-z

DOI

http://dx.doi.org/10.1038/s41598-022-12467-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1148055454

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/35597800


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curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-022-12467-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-022-12467-z'


 

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