Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex View Full Text


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Article Info

DATE

2021-06-15

AUTHORS

M. Provansal, G. Labernède, C. Joffrois, A. Rizkallah, R. Goulet, M. Valet, W. Deschamps, U. Ferrari, A. Chaffiol, D. Dalkara, J. A. Sahel, M. Tanter, S. Picaud, G. Gauvain, F. Arcizet

ABSTRACT

Optogenetics has revolutionized neurosciences by allowing fine control of neuronal activity. An important aspect for this control is assessing the activation and/or adjusting the stimulation, which requires imaging the entire volume of optogenetically-induced neuronal activity. An ideal technique for this aim is fUS imaging, which allows one to generate brain-wide activation maps with submesoscopic spatial resolution. However, optical stimulation of the brain with blue light might lead to non-specific activations at high irradiances. fUS imaging of optogenetic activations can be obtained at these wavelengths using lower light power (< 2mW) but it limits the depth of directly activatable neurons from the cortical surface. Our main goal was to report that we can detect specific optogenetic activations in V1 even in deep layers following stimulation at the cortical surface. Here, we show the possibility to detect deep optogenetic activations in anesthetized rats expressing the red-shifted opsin ChrimsonR in V1 using fUS imaging. We demonstrate the optogenetic specificity of these activations and their neuronal origin with electrophysiological recordings. Finally, we show that the optogenetic response initiated in V1 spreads to downstream (LGN) and upstream (V2) visual areas. More... »

PAGES

12603

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-021-91972-z

    DOI

    http://dx.doi.org/10.1038/s41598-021-91972-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1138858204

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/34131223


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    336 schema:name Department of Ophthalmology and Vitreo-Retinal Diseases, Fondation Ophtalmologique Rothschild, 75012, Paris, France
    337 Department of Ophthalmology, The University of Pittsburgh School of Medicine, 15213, Pittsburgh, PA, USA
    338 Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012, Paris, France
    339 rdf:type schema:Organization
    340 grid-institutes:grid.418241.a schema:alternateName Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012, Paris, France
    341 schema:name Sorbonne Université, INSERM, CNRS, Institut de la Vision, 75012, Paris, France
    342 rdf:type schema:Organization
     




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