Ontology type: schema:ScholarlyArticle Open Access: True
2021-03-03
AUTHORSErin S. Gloag, Daniel J. Wozniak, Paul Stoodley, Luanne Hall-Stoodley
ABSTRACTMycobacterium abscessus is emerging as a cause of recalcitrant chronic pulmonary infections, particularly in people with cystic fibrosis (CF). Biofilm formation has been implicated in the pathology of this organism, however the role of biofilm formation in infection is unclear. Two colony-variants of M. abscessus are routinely isolated from CF samples, smooth (MaSm) and rough (MaRg). These two variants display distinct colony morphologies due to the presence (MaSm) or absence (MaRg) of cell wall glycopeptidolipids (GPLs). We hypothesized that MaSm and MaRg variant biofilms might have different mechanical properties. To test this hypothesis, we performed uniaxial mechanical indentation, and shear rheometry on MaSm and MaRg colony-biofilms. We identified that MaRg biofilms were significantly stiffer than MaSm under a normal force, while MaSm biofilms were more pliant compared to MaRg, under both normal and shear forces. Furthermore, using theoretical indices of mucociliary and cough clearance, we identified that M. abscessus biofilms may be more resistant to mechanical forms of clearance from the lung, compared to another common pulmonary pathogen, Pseudomonas aeruginosa. Thus, the mechanical properties of M. abscessus biofilms may contribute to the persistent nature of pulmonary infections caused by this organism. More... »
PAGES5020
http://scigraph.springernature.com/pub.10.1038/s41598-021-84525-x
DOIhttp://dx.doi.org/10.1038/s41598-021-84525-x
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1135873716
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/33658597
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Microbiology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biofilms",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biomechanical Phenomena",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Wall",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Elasticity",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Glycopeptides",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lipopeptides",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mycobacterium abscessus",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Pseudomonas aeruginosa",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rheology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Shear Strength",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Viscosity",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Microbial Infection and Immunity, The Ohio State University, 711 Biomedical Research Tower, 460 W 12th Avenue, Columbus, OH, USA",
"id": "http://www.grid.ac/institutes/grid.261331.4",
"name": [
"Department of Microbial Infection and Immunity, The Ohio State University, 711 Biomedical Research Tower, 460 W 12th Avenue, Columbus, OH, USA"
],
"type": "Organization"
},
"familyName": "Gloag",
"givenName": "Erin S.",
"id": "sg:person.01107523244.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107523244.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbiology, The Ohio State University, 43210, Columbus, OH, USA",
"id": "http://www.grid.ac/institutes/grid.261331.4",
"name": [
"Department of Microbial Infection and Immunity, The Ohio State University, 711 Biomedical Research Tower, 460 W 12th Avenue, Columbus, OH, USA",
"Department of Microbiology, The Ohio State University, 43210, Columbus, OH, USA"
],
"type": "Organization"
},
"familyName": "Wozniak",
"givenName": "Daniel J.",
"id": "sg:person.01303663451.27",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303663451.27"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Biofilm Innovation Centre (NBIC) and National Centre for Advanced Tribology at Southampton (nCATS), University of Southampton, SO17 1BJ, Southampton, UK",
"id": "http://www.grid.ac/institutes/grid.5491.9",
"name": [
"Department of Microbial Infection and Immunity, The Ohio State University, 711 Biomedical Research Tower, 460 W 12th Avenue, Columbus, OH, USA",
"Department of Orthopedics, The Ohio State University, 43210, Columbus, OH, USA",
"National Biofilm Innovation Centre (NBIC) and National Centre for Advanced Tribology at Southampton (nCATS), University of Southampton, SO17 1BJ, Southampton, UK"
],
"type": "Organization"
},
"familyName": "Stoodley",
"givenName": "Paul",
"id": "sg:person.01316722264.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316722264.07"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Microbial Infection and Immunity, The Ohio State University, 711 Biomedical Research Tower, 460 W 12th Avenue, Columbus, OH, USA",
"id": "http://www.grid.ac/institutes/grid.261331.4",
"name": [
"Department of Microbial Infection and Immunity, The Ohio State University, 711 Biomedical Research Tower, 460 W 12th Avenue, Columbus, OH, USA"
],
"type": "Organization"
},
"familyName": "Hall-Stoodley",
"givenName": "Luanne",
"id": "sg:person.0607041661.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607041661.06"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/s41522-016-0007-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1074191638",
"https://doi.org/10.1038/s41522-016-0007-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-018-28009-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1105019208",
"https://doi.org/10.1038/s41598-018-28009-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35037627",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040445713",
"https://doi.org/10.1038/35037627"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.2019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026706357",
"https://doi.org/10.1038/nmeth.2019"
],
"type": "CreativeWork"
}
],
"datePublished": "2021-03-03",
"datePublishedReg": "2021-03-03",
"description": "Mycobacterium abscessus is emerging as a cause of recalcitrant chronic pulmonary infections, particularly in people with cystic fibrosis (CF). Biofilm formation has been implicated in the pathology of this organism, however the role of biofilm formation in infection is unclear. Two colony-variants of M. abscessus are routinely isolated from CF samples, smooth (MaSm) and rough (MaRg). These two variants display distinct colony morphologies due to the presence (MaSm) or absence (MaRg) of cell wall glycopeptidolipids (GPLs). We hypothesized that MaSm and MaRg variant biofilms might have different mechanical properties. To test this hypothesis, we performed uniaxial mechanical indentation, and shear rheometry on MaSm and MaRg colony-biofilms. We identified that MaRg biofilms were significantly stiffer than MaSm under a normal force, while MaSm biofilms were more pliant compared to MaRg, under both normal and shear forces. Furthermore, using theoretical indices of mucociliary and cough\u00a0clearance, we identified that M. abscessus biofilms may be more resistant to mechanical forms of clearance from the lung, compared to another common pulmonary pathogen, Pseudomonas aeruginosa. Thus, the mechanical properties of M. abscessus biofilms may contribute to the persistent nature of pulmonary infections caused by this organism.",
"genre": "article",
"id": "sg:pub.10.1038/s41598-021-84525-x",
"inLanguage": "en",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.8384083",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.7169920",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.7083392",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.100073342",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.7751836",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1045337",
"issn": [
"2045-2322"
],
"name": "Scientific Reports",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "11"
}
],
"keywords": [
"chronic pulmonary infection",
"pulmonary infection",
"cystic fibrosis",
"M. abscessus biofilms",
"common pulmonary pathogens",
"pulmonary pathogens",
"Mycobacterium abscessus",
"M. abscessus",
"infection",
"abscessus",
"cell wall glycopeptidolipids",
"Pseudomonas aeruginosa",
"clearance",
"biofilm formation",
"Mycobacterium abscessus biofilms",
"mucociliary",
"fibrosis",
"distinct colony morphology",
"lung",
"CF samples",
"pathology",
"persistent nature",
"glycopeptidolipids",
"cause",
"aeruginosa",
"MASM",
"pathogens",
"biofilms",
"mechanical indentation",
"index",
"colony morphology",
"absence",
"variants",
"role",
"people",
"organisms",
"hypothesis",
"presence",
"samples",
"MARG",
"formation",
"shear force",
"form",
"theoretical indices",
"morphology",
"mechanical form",
"viscoelastic properties",
"nature",
"force",
"properties",
"indentation",
"different mechanical properties",
"recalcitrance",
"mechanical properties",
"normal force",
"shear rheometry",
"rheometry"
],
"name": "Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections",
"pagination": "5020",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1135873716"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41598-021-84525-x"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"33658597"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41598-021-84525-x",
"https://app.dimensions.ai/details/publication/pub.1135873716"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-10T10:30",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/article/article_881.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/s41598-021-84525-x"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-021-84525-x'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-021-84525-x'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-021-84525-x'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-021-84525-x'
This table displays all metadata directly associated to this object as RDF triples.
220 TRIPLES
22 PREDICATES
99 URIs
87 LITERALS
19 BLANK NODES