1H-NMR metabolomics reveals a multitarget action of Crithmum maritimum ethyl acetate extract in inhibiting hepatocellular carcinoma cell growth View Full Text


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Article Info

DATE

2021-01-13

AUTHORS

Davide Gnocchi, Laura Del Coco, Chiara Roberta Girelli, Francesca Castellaneta, Gianluigi Cesari, Carlo Sabbà, Francesco Paolo Fanizzi, Antonio Mazzocca

ABSTRACT

Hepatocellular carcinoma (HCC) is nowadays the sixth cause of tumour-related deceases worldwide, estimated to become the third in Western countries by 2030. New drugs for HCC treatment still have many adverse effects. Several lines of evidence indicate that plant metabolites offer concrete opportunities for developing new therapeutic strategies for many diseases, including cancer. We previously reported that ethyl acetate extract of a spontaneous edible plant harvested in Apulia, Crithmum maritimum, significantly inhibited cell growth in HCC cells. By 1H-NMR spectroscopy, here we show that Crithmum maritimum ethyl acetate extract counteracts the Warburg effect, by reducing intracellular lactate, inhibits protein anabolism, by decreasing amino acid level, and affects membrane biosynthesis by lowering choline and phosphocholine. Also, we observed an effect on lipid homeostasis, with a reduction in triglycerides, cholesterol, monounsaturated fatty acids (MUFA), and diunsaturated fatty acids (DUFA), and an increase in polyunsaturated fatty acids (PUFA). Taken together, these data demonstrate that Crithmum maritimum-induced cytostasis is exerted through a multi-effect action, targeting key metabolic processes in HCC cells. Overall, our findings highlight the role of Crithmum maritimum as a promising tool for the prevention and the improvement of the therapeutic options for HCC and other types of tumours. More... »

PAGES

1259

References to SciGraph publications

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  • 2018-06-20. NMR-based Metabolomic Techniques Identify the Toxicity of Emodin in HepG2 Cells in SCIENTIFIC REPORTS
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1038/s41598-020-78867-1

    DOI

    http://dx.doi.org/10.1038/s41598-020-78867-1

    DIMENSIONS

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    PUBMED

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    39 HCC treatment
    40 Warburg effect
    41 Western countries
    42 acetate extract
    43 acid
    44 acid levels
    45 action
    46 adverse effects
    47 amino acid levels
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    50 cancer
    51 carcinoma
    52 carcinoma cell growth
    53 cause
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    55 cells
    56 cholesterol
    57 choline
    58 concrete opportunities
    59 counteracts
    60 countries
    61 cytostasis
    62 data
    63 decease
    64 disease
    65 drugs
    66 edible plants
    67 effect
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    69 evidence
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    71 fatty acids
    72 findings
    73 growth
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    75 hepatocellular carcinoma cell growth
    76 homeostasis
    77 improvement
    78 increase
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    80 key metabolic processes
    81 lactate
    82 levels
    83 lines
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    85 lipid homeostasis
    86 maritimum
    87 membrane biosynthesis
    88 metabolic processes
    89 metabolites
    90 metabolomics
    91 multitarget action
    92 new drugs
    93 new therapeutic strategies
    94 opportunities
    95 options
    96 phosphocholine
    97 plant metabolites
    98 plants
    99 prevention
    100 process
    101 promising tool
    102 protein anabolism
    103 reduction
    104 role
    105 sixth cause
    106 spectroscopy
    107 strategies
    108 therapeutic options
    109 therapeutic strategies
    110 tool
    111 treatment
    112 triglycerides
    113 tumors
    114 types
    115 types of tumors
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