Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-12-09

AUTHORS

Matthew Sorensen, Courtney E. Chandler, Francesca M. Gardner, Salma Ramadan, Prasanna D. Khot, Lisa M. Leung, Christine E. Farrance, David R. Goodlett, Robert K. Ernst, Erik Nilsson

ABSTRACT

Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin. More... »

PAGES

21536

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-020-78401-3

DOI

http://dx.doi.org/10.1038/s41598-020-78401-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1133339203

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33299017


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colistin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance, Bacterial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gram-Negative Bacteria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gram-Positive Bacteria", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lipids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Pataigin, LLC, Seattle, WA, USA", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Pataigin, LLC, Seattle, WA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sorensen", 
        "givenName": "Matthew", 
        "id": "sg:person.012476334501.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012476334501.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Johns Hopkins University, 21205, Baltimore, MD, USA", 
          "id": "http://www.grid.ac/institutes/grid.21107.35", 
          "name": [
            "Pataigin, LLC, Seattle, WA, USA", 
            "University of Maryland, Baltimore, 21201, Baltimore, MD, USA", 
            "Johns Hopkins University, 21205, Baltimore, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chandler", 
        "givenName": "Courtney E.", 
        "id": "sg:person.014747502255.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014747502255.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Maryland, Baltimore, 21201, Baltimore, MD, USA", 
          "id": "http://www.grid.ac/institutes/grid.411024.2", 
          "name": [
            "Pataigin, LLC, Seattle, WA, USA", 
            "University of Maryland, Baltimore, 21201, Baltimore, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gardner", 
        "givenName": "Francesca M.", 
        "id": "sg:person.014145752227.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014145752227.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pataigin, LLC, Seattle, WA, USA", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Pataigin, LLC, Seattle, WA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ramadan", 
        "givenName": "Salma", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles River Laboratories, Inc., 19711, Newark, DE, USA", 
          "id": "http://www.grid.ac/institutes/grid.280920.1", 
          "name": [
            "Charles River Laboratories, Inc., 19711, Newark, DE, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Khot", 
        "givenName": "Prasanna D.", 
        "id": "sg:person.01063532117.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063532117.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "U.S. Food and Drug Administration, 20993, Silver Spring, MD, USA", 
          "id": "http://www.grid.ac/institutes/grid.417587.8", 
          "name": [
            "Maryland Department of Health and Mental Hygiene, 21205, Baltimore, MD, USA", 
            "U.S. Food and Drug Administration, 20993, Silver Spring, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Leung", 
        "givenName": "Lisa M.", 
        "id": "sg:person.013070770133.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013070770133.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charles River Laboratories, Inc., 19711, Newark, DE, USA", 
          "id": "http://www.grid.ac/institutes/grid.280920.1", 
          "name": [
            "Charles River Laboratories, Inc., 19711, Newark, DE, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Farrance", 
        "givenName": "Christine E.", 
        "id": "sg:person.0717455702.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717455702.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "International Centre for Cancer Vaccine Science, University of Gdansk, Gda\u0144sk, Poland", 
          "id": "http://www.grid.ac/institutes/grid.8585.0", 
          "name": [
            "University of Maryland, Baltimore, 21201, Baltimore, MD, USA", 
            "International Centre for Cancer Vaccine Science, University of Gdansk, Gda\u0144sk, Poland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Goodlett", 
        "givenName": "David R.", 
        "id": "sg:person.01044126157.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044126157.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Maryland, Baltimore, 21201, Baltimore, MD, USA", 
          "id": "http://www.grid.ac/institutes/grid.411024.2", 
          "name": [
            "University of Maryland, Baltimore, 21201, Baltimore, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ernst", 
        "givenName": "Robert K.", 
        "id": "sg:person.01105703045.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105703045.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Pataigin, LLC, Seattle, WA, USA", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Pataigin, LLC, Seattle, WA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nilsson", 
        "givenName": "Erik", 
        "id": "sg:person.010434752455.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010434752455.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/s41579-018-0098-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107667571", 
          "https://doi.org/10.1038/s41579-018-0098-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-74215-6_9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043843840", 
          "https://doi.org/10.1007/978-3-642-74215-6_9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10096-014-2115-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028766052", 
          "https://doi.org/10.1007/s10096-014-2115-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10096-013-2031-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014860297", 
          "https://doi.org/10.1007/s10096-013-2031-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-90-481-9815-3_7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049908814", 
          "https://doi.org/10.1007/978-90-481-9815-3_7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-020-61890-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1125753607", 
          "https://doi.org/10.1038/s41598-020-61890-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-04793-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090807679", 
          "https://doi.org/10.1038/s41598-017-04793-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1016/j.jasms.2007.03.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021652196", 
          "https://doi.org/10.1016/j.jasms.2007.03.008"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2020-12-09", 
    "datePublishedReg": "2020-12-09", 
    "description": "Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/s41598-020-78401-3", 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3805765", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.9020071", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3492861", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "10"
      }
    ], 
    "keywords": [
      "fungal species", 
      "microbial identification", 
      "matrix-assisted laser desorption/ionization time", 
      "Gram-negative bacteria", 
      "lipid biomarker analysis", 
      "laser desorption/ionization time", 
      "desorption/ionization time", 
      "rapid microbial identification", 
      "microbial lipids", 
      "membrane lipids", 
      "MALDI-TOF MS", 
      "ionization time", 
      "species", 
      "lipid extraction methods", 
      "Recent studies", 
      "rapid infection diagnosis", 
      "lipids", 
      "lipid extraction", 
      "identification", 
      "antimicrobial resistance", 
      "bacteria", 
      "selective biomarkers", 
      "assays", 
      "clinical assays", 
      "resistance", 
      "resistance detection", 
      "biomarker analysis", 
      "flats", 
      "modification", 
      "biomarkers", 
      "MS", 
      "analysis", 
      "novel", 
      "workflow", 
      "extraction method", 
      "study", 
      "significant hands", 
      "time", 
      "treatment", 
      "extraction", 
      "direct adoption", 
      "detection", 
      "surface", 
      "hand", 
      "method", 
      "plate", 
      "practical procedure", 
      "stewardship", 
      "patient treatment", 
      "technique", 
      "procedure", 
      "analysis techniques", 
      "outcomes", 
      "patient outcomes", 
      "adoption", 
      "diagnosis", 
      "antimicrobial stewardship", 
      "target extraction", 
      "infection diagnosis"
    ], 
    "name": "Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids", 
    "pagination": "21536", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1133339203"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-020-78401-3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "33299017"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-020-78401-3", 
      "https://app.dimensions.ai/details/publication/pub.1133339203"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:41", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_853.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/s41598-020-78401-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-78401-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-78401-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-78401-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-78401-3'


 

This table displays all metadata directly associated to this object as RDF triples.

267 TRIPLES      21 PREDICATES      99 URIs      83 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-020-78401-3 schema:about N0ef25e5846f14e74a7d263edc4eaf06b
2 N3f6eba8053294fb4a21ef552fa16af4b
3 N4660d075b43e4188bafc513d63bbd5ba
4 Nb90a31952935460a9fea66b002ff28d2
5 Nc8152e6fba944568a57ad5cfda522a08
6 Nda8732dea7b9419fb92864462a80824a
7 Nfce2fc81d3f548589c5e2fac9bbf3ef8
8 anzsrc-for:06
9 anzsrc-for:0605
10 schema:author Nc0d03825ace641a89a81c487d009887a
11 schema:citation sg:pub.10.1007/978-3-642-74215-6_9
12 sg:pub.10.1007/978-90-481-9815-3_7
13 sg:pub.10.1007/s10096-013-2031-5
14 sg:pub.10.1007/s10096-014-2115-x
15 sg:pub.10.1016/j.jasms.2007.03.008
16 sg:pub.10.1038/s41579-018-0098-9
17 sg:pub.10.1038/s41598-017-04793-4
18 sg:pub.10.1038/s41598-020-61890-7
19 schema:datePublished 2020-12-09
20 schema:datePublishedReg 2020-12-09
21 schema:description Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.
22 schema:genre article
23 schema:isAccessibleForFree true
24 schema:isPartOf Na7b1494cb56e47a996bc3fed39998480
25 Nd177859d8c5f4a799635808dd56cf751
26 sg:journal.1045337
27 schema:keywords Gram-negative bacteria
28 MALDI-TOF MS
29 MS
30 Recent studies
31 adoption
32 analysis
33 analysis techniques
34 antimicrobial resistance
35 antimicrobial stewardship
36 assays
37 bacteria
38 biomarker analysis
39 biomarkers
40 clinical assays
41 desorption/ionization time
42 detection
43 diagnosis
44 direct adoption
45 extraction
46 extraction method
47 flats
48 fungal species
49 hand
50 identification
51 infection diagnosis
52 ionization time
53 laser desorption/ionization time
54 lipid biomarker analysis
55 lipid extraction
56 lipid extraction methods
57 lipids
58 matrix-assisted laser desorption/ionization time
59 membrane lipids
60 method
61 microbial identification
62 microbial lipids
63 modification
64 novel
65 outcomes
66 patient outcomes
67 patient treatment
68 plate
69 practical procedure
70 procedure
71 rapid infection diagnosis
72 rapid microbial identification
73 resistance
74 resistance detection
75 selective biomarkers
76 significant hands
77 species
78 stewardship
79 study
80 surface
81 target extraction
82 technique
83 time
84 treatment
85 workflow
86 schema:name Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
87 schema:pagination 21536
88 schema:productId N48de1cea43204bf78936559d37cf03bf
89 N72417c7bfe4c4872ba7c9570a1fa871e
90 Ne27b22b57ab54951bbf765362abd78d3
91 schema:sameAs https://app.dimensions.ai/details/publication/pub.1133339203
92 https://doi.org/10.1038/s41598-020-78401-3
93 schema:sdDatePublished 2022-12-01T06:41
94 schema:sdLicense https://scigraph.springernature.com/explorer/license/
95 schema:sdPublisher Neaa4ee2b124645b3b5a163980b0bf9ab
96 schema:url https://doi.org/10.1038/s41598-020-78401-3
97 sgo:license sg:explorer/license/
98 sgo:sdDataset articles
99 rdf:type schema:ScholarlyArticle
100 N00d9a90ffbe74d02bf99911cf797c38f schema:affiliation grid-institutes:None
101 schema:familyName Ramadan
102 schema:givenName Salma
103 rdf:type schema:Person
104 N0ef25e5846f14e74a7d263edc4eaf06b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Biomarkers
106 rdf:type schema:DefinedTerm
107 N25a155181fbc40a1ab796a7f04d32ac0 rdf:first sg:person.0717455702.15
108 rdf:rest N28765428f77042fa839bcd0b2de4cc07
109 N28765428f77042fa839bcd0b2de4cc07 rdf:first sg:person.01044126157.13
110 rdf:rest N2d183938165e4bd086f3676b3a36211b
111 N2945a81d794f419f83cc6716e389d566 rdf:first sg:person.014145752227.53
112 rdf:rest N59b6299f15574afcba8d712f9095ff95
113 N2d183938165e4bd086f3676b3a36211b rdf:first sg:person.01105703045.52
114 rdf:rest Naa8876330c014853977539ac19eb0ea4
115 N3f6eba8053294fb4a21ef552fa16af4b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Lipids
117 rdf:type schema:DefinedTerm
118 N4660d075b43e4188bafc513d63bbd5ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Gram-Negative Bacteria
120 rdf:type schema:DefinedTerm
121 N48de1cea43204bf78936559d37cf03bf schema:name dimensions_id
122 schema:value pub.1133339203
123 rdf:type schema:PropertyValue
124 N59b6299f15574afcba8d712f9095ff95 rdf:first N00d9a90ffbe74d02bf99911cf797c38f
125 rdf:rest Na054ba5426e34f2f89caa15f12af9624
126 N72417c7bfe4c4872ba7c9570a1fa871e schema:name pubmed_id
127 schema:value 33299017
128 rdf:type schema:PropertyValue
129 N9124162e9e164c5dace5a3792377a2dd rdf:first sg:person.013070770133.50
130 rdf:rest N25a155181fbc40a1ab796a7f04d32ac0
131 Na054ba5426e34f2f89caa15f12af9624 rdf:first sg:person.01063532117.11
132 rdf:rest N9124162e9e164c5dace5a3792377a2dd
133 Na7b1494cb56e47a996bc3fed39998480 schema:issueNumber 1
134 rdf:type schema:PublicationIssue
135 Naa8876330c014853977539ac19eb0ea4 rdf:first sg:person.010434752455.05
136 rdf:rest rdf:nil
137 Nb90a31952935460a9fea66b002ff28d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Gram-Positive Bacteria
139 rdf:type schema:DefinedTerm
140 Nc0d03825ace641a89a81c487d009887a rdf:first sg:person.012476334501.47
141 rdf:rest Necec115a4c704385a1a3666d6557804f
142 Nc8152e6fba944568a57ad5cfda522a08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
144 rdf:type schema:DefinedTerm
145 Nd177859d8c5f4a799635808dd56cf751 schema:volumeNumber 10
146 rdf:type schema:PublicationVolume
147 Nda8732dea7b9419fb92864462a80824a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Colistin
149 rdf:type schema:DefinedTerm
150 Ne27b22b57ab54951bbf765362abd78d3 schema:name doi
151 schema:value 10.1038/s41598-020-78401-3
152 rdf:type schema:PropertyValue
153 Neaa4ee2b124645b3b5a163980b0bf9ab schema:name Springer Nature - SN SciGraph project
154 rdf:type schema:Organization
155 Necec115a4c704385a1a3666d6557804f rdf:first sg:person.014747502255.58
156 rdf:rest N2945a81d794f419f83cc6716e389d566
157 Nfce2fc81d3f548589c5e2fac9bbf3ef8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Drug Resistance, Bacterial
159 rdf:type schema:DefinedTerm
160 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
161 schema:name Biological Sciences
162 rdf:type schema:DefinedTerm
163 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
164 schema:name Microbiology
165 rdf:type schema:DefinedTerm
166 sg:grant.3492861 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-78401-3
167 rdf:type schema:MonetaryGrant
168 sg:grant.3805765 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-78401-3
169 rdf:type schema:MonetaryGrant
170 sg:grant.9020071 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-78401-3
171 rdf:type schema:MonetaryGrant
172 sg:journal.1045337 schema:issn 2045-2322
173 schema:name Scientific Reports
174 schema:publisher Springer Nature
175 rdf:type schema:Periodical
176 sg:person.010434752455.05 schema:affiliation grid-institutes:None
177 schema:familyName Nilsson
178 schema:givenName Erik
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010434752455.05
180 rdf:type schema:Person
181 sg:person.01044126157.13 schema:affiliation grid-institutes:grid.8585.0
182 schema:familyName Goodlett
183 schema:givenName David R.
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044126157.13
185 rdf:type schema:Person
186 sg:person.01063532117.11 schema:affiliation grid-institutes:grid.280920.1
187 schema:familyName Khot
188 schema:givenName Prasanna D.
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063532117.11
190 rdf:type schema:Person
191 sg:person.01105703045.52 schema:affiliation grid-institutes:grid.411024.2
192 schema:familyName Ernst
193 schema:givenName Robert K.
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105703045.52
195 rdf:type schema:Person
196 sg:person.012476334501.47 schema:affiliation grid-institutes:None
197 schema:familyName Sorensen
198 schema:givenName Matthew
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012476334501.47
200 rdf:type schema:Person
201 sg:person.013070770133.50 schema:affiliation grid-institutes:grid.417587.8
202 schema:familyName Leung
203 schema:givenName Lisa M.
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013070770133.50
205 rdf:type schema:Person
206 sg:person.014145752227.53 schema:affiliation grid-institutes:grid.411024.2
207 schema:familyName Gardner
208 schema:givenName Francesca M.
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014145752227.53
210 rdf:type schema:Person
211 sg:person.014747502255.58 schema:affiliation grid-institutes:grid.21107.35
212 schema:familyName Chandler
213 schema:givenName Courtney E.
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014747502255.58
215 rdf:type schema:Person
216 sg:person.0717455702.15 schema:affiliation grid-institutes:grid.280920.1
217 schema:familyName Farrance
218 schema:givenName Christine E.
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717455702.15
220 rdf:type schema:Person
221 sg:pub.10.1007/978-3-642-74215-6_9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043843840
222 https://doi.org/10.1007/978-3-642-74215-6_9
223 rdf:type schema:CreativeWork
224 sg:pub.10.1007/978-90-481-9815-3_7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049908814
225 https://doi.org/10.1007/978-90-481-9815-3_7
226 rdf:type schema:CreativeWork
227 sg:pub.10.1007/s10096-013-2031-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014860297
228 https://doi.org/10.1007/s10096-013-2031-5
229 rdf:type schema:CreativeWork
230 sg:pub.10.1007/s10096-014-2115-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028766052
231 https://doi.org/10.1007/s10096-014-2115-x
232 rdf:type schema:CreativeWork
233 sg:pub.10.1016/j.jasms.2007.03.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021652196
234 https://doi.org/10.1016/j.jasms.2007.03.008
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/s41579-018-0098-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107667571
237 https://doi.org/10.1038/s41579-018-0098-9
238 rdf:type schema:CreativeWork
239 sg:pub.10.1038/s41598-017-04793-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090807679
240 https://doi.org/10.1038/s41598-017-04793-4
241 rdf:type schema:CreativeWork
242 sg:pub.10.1038/s41598-020-61890-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1125753607
243 https://doi.org/10.1038/s41598-020-61890-7
244 rdf:type schema:CreativeWork
245 grid-institutes:None schema:alternateName Pataigin, LLC, Seattle, WA, USA
246 schema:name Pataigin, LLC, Seattle, WA, USA
247 rdf:type schema:Organization
248 grid-institutes:grid.21107.35 schema:alternateName Johns Hopkins University, 21205, Baltimore, MD, USA
249 schema:name Johns Hopkins University, 21205, Baltimore, MD, USA
250 Pataigin, LLC, Seattle, WA, USA
251 University of Maryland, Baltimore, 21201, Baltimore, MD, USA
252 rdf:type schema:Organization
253 grid-institutes:grid.280920.1 schema:alternateName Charles River Laboratories, Inc., 19711, Newark, DE, USA
254 schema:name Charles River Laboratories, Inc., 19711, Newark, DE, USA
255 rdf:type schema:Organization
256 grid-institutes:grid.411024.2 schema:alternateName University of Maryland, Baltimore, 21201, Baltimore, MD, USA
257 schema:name Pataigin, LLC, Seattle, WA, USA
258 University of Maryland, Baltimore, 21201, Baltimore, MD, USA
259 rdf:type schema:Organization
260 grid-institutes:grid.417587.8 schema:alternateName U.S. Food and Drug Administration, 20993, Silver Spring, MD, USA
261 schema:name Maryland Department of Health and Mental Hygiene, 21205, Baltimore, MD, USA
262 U.S. Food and Drug Administration, 20993, Silver Spring, MD, USA
263 rdf:type schema:Organization
264 grid-institutes:grid.8585.0 schema:alternateName International Centre for Cancer Vaccine Science, University of Gdansk, Gdańsk, Poland
265 schema:name International Centre for Cancer Vaccine Science, University of Gdansk, Gdańsk, Poland
266 University of Maryland, Baltimore, 21201, Baltimore, MD, USA
267 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...