Cadherin repeat 5 mutation associated with Bt resistance in a field-derived strain of pink bollworm View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-10-08

AUTHORS

Ling Wang, Yuemin Ma, Wei Wei, Peng Wan, Kaiyu Liu, Min Xu, Shengbo Cong, Jintao Wang, Dong Xu, Yutao Xiao, Xianchun Li, Bruce E. Tabashnik, Kongming Wu

ABSTRACT

Evolution of resistance by pests reduces the benefits of transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt). Here we analyzed resistance to Bt toxin Cry1Ac in a field-derived strain of pink bollworm (Pectinophora gossypiella), a global pest of cotton. We discovered that the r14 allele of the pink bollworm cadherin gene (PgCad1) has a 234-bp insertion in exon 12 encoding a mutant PgCad1 protein that lacks 36 amino acids in cadherin repeat 5 (CR5). A strain homozygous for this allele had 237-fold resistance to Cry1Ac, 1.8-fold cross-resistance to Cry2Ab, and developed from neonate to adult on Bt cotton producing Cry1Ac. Inheritance of resistance to Cry1Ac was recessive and tightly linked with r14. PgCad1 transcript abundance in midgut tissues did not differ between resistant and susceptible larvae. Toxicity of Cry1Ac to transformed insect cells was lower for cells expressing r14 than for cells expressing wild-type PgCad1. Wild-type PgCad1 was transported to the cell membrane, whereas PgCad1 produced by r14 was not. In larval midgut tissue, PgCad1 protein occurred primarily on the brush border membrane only in susceptible larvae. The results imply r14 mediates pink bollworm resistance to Cry1Ac by reduced translation, increased degradation, and/or mislocalization of cadherin. More... »

PAGES

16840

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-020-74102-z

DOI

http://dx.doi.org/10.1038/s41598-020-74102-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1131505049

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33033325


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28 schema:description Evolution of resistance by pests reduces the benefits of transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt). Here we analyzed resistance to Bt toxin Cry1Ac in a field-derived strain of pink bollworm (Pectinophora gossypiella), a global pest of cotton. We discovered that the r14 allele of the pink bollworm cadherin gene (PgCad1) has a 234-bp insertion in exon 12 encoding a mutant PgCad1 protein that lacks 36 amino acids in cadherin repeat 5 (CR5). A strain homozygous for this allele had 237-fold resistance to Cry1Ac, 1.8-fold cross-resistance to Cry2Ab, and developed from neonate to adult on Bt cotton producing Cry1Ac. Inheritance of resistance to Cry1Ac was recessive and tightly linked with r14. PgCad1 transcript abundance in midgut tissues did not differ between resistant and susceptible larvae. Toxicity of Cry1Ac to transformed insect cells was lower for cells expressing r14 than for cells expressing wild-type PgCad1. Wild-type PgCad1 was transported to the cell membrane, whereas PgCad1 produced by r14 was not. In larval midgut tissue, PgCad1 protein occurred primarily on the brush border membrane only in susceptible larvae. The results imply r14 mediates pink bollworm resistance to Cry1Ac by reduced translation, increased degradation, and/or mislocalization of cadherin.
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34 schema:keywords Bacillus thuringiensis
35 Bt cotton
36 Bt resistance
37 Bt toxins
38 Cry1Ac
39 Cry2Ab
40 PgCad1
41 R14
42 abundance
43 acid
44 adults
45 alleles
46 amino acids
47 benefits
48 bollworm
49 bollworm resistance
50 border membrane
51 brush border membrane
52 cadherin
53 cadherin gene
54 cell membrane
55 cells
56 cotton
57 crops
58 degradation
59 evolution
60 evolution of resistance
61 exon 12
62 field-derived strain
63 genes
64 global pest
65 inheritance
66 inheritance of resistance
67 insect cells
68 insecticidal proteins
69 insertion
70 larvae
71 larval midgut tissue
72 membrane
73 midgut tissue
74 mislocalization
75 mutations
76 neonates
77 pests
78 pink bollworm
79 pink bollworm resistance
80 protein
81 reduced translation
82 repeat 5
83 resistance
84 results
85 strains
86 susceptible larvae
87 thuringiensis
88 tissue
89 toxicity
90 toxicity of Cry1Ac
91 toxin
92 transcript abundance
93 transgenic crops
94 translation
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