Ontology type: schema:ScholarlyArticle Open Access: True
2020-09-28
AUTHORSYoshiaki Kinosita, Tsubasa Ishida, Myu Yoshida, Rie Ito, Yusuke V. Morimoto, Kazuki Goto, Richard M. Berry, Takayuki Nishizaka, Yoshiyuki Sowa
ABSTRACTMost motile bacteria are propelled by rigid, helical, flagellar filaments and display distinct swimming patterns to explore their favorable environments. Escherichia coli cells have a reversible rotary motor at the base of each filament. They exhibit a run-tumble swimming pattern, driven by switching of the rotational direction, which causes polymorphic flagellar transformation. Here we report a novel swimming mode in E. coli ATCC10798, which is one of the original K-12 clones. High-speed tracking of single ATCC10798 cells showed forward and backward swimming with an average turning angle of 150°. The flagellar helicity remained right-handed with a 1.3 μm pitch and 0.14 μm helix radius, which is consistent with the feature of a curly type, regardless of motor switching; the flagella of ATCC10798 did not show polymorphic transformation. The torque and rotational switching of the motor was almost identical to the E. coli W3110 strain, which is a derivative of K-12 and a wild-type for chemotaxis. The single point mutation of N87K in FliC, one of the filament subunits, is critical to the change in flagellar morphology and swimming pattern, and lack of flagellar polymorphism. E. coli cells expressing FliC(N87K) sensed ascending a chemotactic gradient in liquid but did not spread on a semi-solid surface. Based on these results, we concluded that a flagellar polymorphism is essential for spreading in structured environments. More... »
PAGES15887
http://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1
DOIhttp://dx.doi.org/10.1038/s41598-020-72429-1
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1131231896
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/32985511
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chemotaxis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Escherichia coli K12",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Flagella",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Models, Biological",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutation",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Present Address: Molecular Physiology Laboratory, RIKEN, Wako, Japan",
"id": "http://www.grid.ac/institutes/grid.7597.c",
"name": [
"Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo, 171-8588 Japan",
"Department of Physics, University of Oxford, Park load, Oxford, OX1 3PU UK",
"Present Address: Molecular Physiology Laboratory, RIKEN, Wako, Japan"
],
"type": "Organization"
},
"familyName": "Kinosita",
"givenName": "Yoshiaki",
"id": "sg:person.016370455126.53",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016370455126.53"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan",
"id": "http://www.grid.ac/institutes/grid.257114.4",
"name": [
"Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan"
],
"type": "Organization"
},
"familyName": "Ishida",
"givenName": "Tsubasa",
"id": "sg:person.014240554547.01",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014240554547.01"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan",
"id": "http://www.grid.ac/institutes/grid.257114.4",
"name": [
"Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan"
],
"type": "Organization"
},
"familyName": "Yoshida",
"givenName": "Myu",
"id": "sg:person.01037221602.77",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037221602.77"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan",
"id": "http://www.grid.ac/institutes/grid.257114.4",
"name": [
"Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan"
],
"type": "Organization"
},
"familyName": "Ito",
"givenName": "Rie",
"id": "sg:person.015036135147.61",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015036135147.61"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka Japan",
"id": "http://www.grid.ac/institutes/grid.258806.1",
"name": [
"Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka Japan"
],
"type": "Organization"
},
"familyName": "Morimoto",
"givenName": "Yusuke V.",
"id": "sg:person.0727414150.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727414150.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo, 171-8588 Japan",
"id": "http://www.grid.ac/institutes/grid.256169.f",
"name": [
"Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo, 171-8588 Japan"
],
"type": "Organization"
},
"familyName": "Goto",
"givenName": "Kazuki",
"id": "sg:person.010235265061.68",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010235265061.68"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physics, University of Oxford, Park load, Oxford, OX1 3PU UK",
"id": "http://www.grid.ac/institutes/grid.4991.5",
"name": [
"Department of Physics, University of Oxford, Park load, Oxford, OX1 3PU UK"
],
"type": "Organization"
},
"familyName": "Berry",
"givenName": "Richard M.",
"id": "sg:person.01300410534.17",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300410534.17"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo, 171-8588 Japan",
"id": "http://www.grid.ac/institutes/grid.256169.f",
"name": [
"Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo, 171-8588 Japan"
],
"type": "Organization"
},
"familyName": "Nishizaka",
"givenName": "Takayuki",
"id": "sg:person.01372020626.32",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372020626.32"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan",
"id": "http://www.grid.ac/institutes/grid.257114.4",
"name": [
"Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, Tokyo, 184-8584 Japan"
],
"type": "Organization"
},
"familyName": "Sowa",
"givenName": "Yoshiyuki",
"id": "sg:person.01047733534.32",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047733534.32"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/srep26147",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001227801",
"https://doi.org/10.1038/srep26147"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/249073a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013527060",
"https://doi.org/10.1038/249073a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nphys2676",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037938190",
"https://doi.org/10.1038/nphys2676"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/245380a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006993053",
"https://doi.org/10.1038/245380a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41467-018-07802-w",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1110559604",
"https://doi.org/10.1038/s41467-018-07802-w"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41467-017-01075-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092152763",
"https://doi.org/10.1038/s41467-017-01075-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/239500a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028194584",
"https://doi.org/10.1038/239500a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb.1774",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000526904",
"https://doi.org/10.1038/nsmb.1774"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/255121a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016034428",
"https://doi.org/10.1038/255121a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature01830",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000565071",
"https://doi.org/10.1038/nature01830"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-018-32686-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107129442",
"https://doi.org/10.1038/s41598-018-32686-7"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-017-16428-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1093031902",
"https://doi.org/10.1038/s41598-017-16428-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ncomms9776",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015996270",
"https://doi.org/10.1038/ncomms9776"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41396-017-0010-z",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1099738979",
"https://doi.org/10.1038/s41396-017-0010-z"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmicrobiol.2016.148",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008438133",
"https://doi.org/10.1038/nmicrobiol.2016.148"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-018-29875-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1105832167",
"https://doi.org/10.1038/s41598-018-29875-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41586-019-1733-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1122331185",
"https://doi.org/10.1038/s41586-019-1733-y"
],
"type": "CreativeWork"
}
],
"datePublished": "2020-09-28",
"datePublishedReg": "2020-09-28",
"description": "Most motile bacteria are propelled by rigid, helical, flagellar filaments and display distinct swimming patterns to explore their favorable environments. Escherichia coli cells have a reversible rotary motor at the base of each filament. They exhibit a run-tumble swimming pattern, driven by switching of the rotational direction, which causes polymorphic flagellar transformation. Here we report a novel swimming mode in E. coli ATCC10798, which is one of the original K-12 clones. High-speed tracking of single ATCC10798 cells showed forward and backward swimming with an average turning angle of 150\u00b0. The flagellar helicity remained right-handed with a 1.3\u00a0\u03bcm pitch and 0.14\u00a0\u03bcm helix radius, which is consistent with the\u00a0feature of a curly type, regardless of motor switching; the flagella of ATCC10798 did not show polymorphic transformation. The torque and rotational switching of the motor was almost identical to the E. coli W3110 strain, which is a derivative of K-12 and a wild-type for chemotaxis. The single point mutation of N87K in FliC, one of the filament subunits, is critical to the change in flagellar morphology and swimming pattern, and lack of flagellar polymorphism. E. coli cells expressing FliC(N87K) sensed ascending a chemotactic gradient in liquid but did not spread on a semi-solid surface. Based on these results, we concluded that a flagellar polymorphism is essential for spreading in structured environments.",
"genre": "article",
"id": "sg:pub.10.1038/s41598-020-72429-1",
"inLanguage": "en",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.4576491",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.5871626",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.7522024",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.8430349",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.5857619",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.5863309",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1045337",
"issn": [
"2045-2322"
],
"name": "Scientific Reports",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "10"
}
],
"keywords": [
"distinct chemotactic behavior",
"N87K",
"chemotactic gradient",
"distinct swimming patterns",
"cells",
"point mutations",
"polymorphism",
"swimming patterns",
"flagellar morphology",
"single point mutation",
"chemotaxis",
"curly types",
"patterns",
"chemotactic behavior",
"mutations",
"Most motile bacteria",
"reversible rotary motor",
"FliC",
"clones",
"lack",
"swimming",
"strains",
"changes",
"motor",
"bacteria",
"subunits",
"backward swimming",
"motile bacteria",
"favorable environment",
"coli cells",
"coli K",
"types",
"filament subunits",
"flagellar filaments",
"Escherichia coli K",
"features",
"results",
"movement",
"filaments",
"flagellar polymorphism",
"derivatives",
"Escherichia coli cells",
"flagella",
"motor switching",
"base",
"morphology",
"semi-solid surfaces",
"behavior",
"rotational direction",
"flagellar transformation",
"environment",
"gradient",
"transformation",
"torque",
"angle",
"mode",
"direction",
"surface",
"tracking",
"swimming modes",
"pitch",
"switching",
"structured environment",
"radius",
"liquid",
"rotary motor",
"high-speed tracking",
"rotational switching",
"helicity",
"polymorphic transformation",
"helix radius",
"run-tumble swimming pattern",
"polymorphic flagellar transformation",
"novel swimming mode",
"coli ATCC10798",
"ATCC10798",
"single ATCC10798 cells",
"ATCC10798 cells",
"flagellar helicity",
"flagella of ATCC10798",
"coli W3110 strain",
"W3110 strain",
"original Escherichia coli K",
"run-tumble movements"
],
"name": "Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements",
"pagination": "15887",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1131231896"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41598-020-72429-1"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"32985511"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41598-020-72429-1",
"https://app.dimensions.ai/details/publication/pub.1131231896"
],
"sdDataset": "articles",
"sdDatePublished": "2022-01-01T18:56",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_838.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/s41598-020-72429-1"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'
This table displays all metadata directly associated to this object as RDF triples.
315 TRIPLES
22 PREDICATES
132 URIs
107 LITERALS
12 BLANK NODES