Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-09-28

AUTHORS

Yoshiaki Kinosita, Tsubasa Ishida, Myu Yoshida, Rie Ito, Yusuke V. Morimoto, Kazuki Goto, Richard M. Berry, Takayuki Nishizaka, Yoshiyuki Sowa

ABSTRACT

Most motile bacteria are propelled by rigid, helical, flagellar filaments and display distinct swimming patterns to explore their favorable environments. Escherichia coli cells have a reversible rotary motor at the base of each filament. They exhibit a run-tumble swimming pattern, driven by switching of the rotational direction, which causes polymorphic flagellar transformation. Here we report a novel swimming mode in E. coli ATCC10798, which is one of the original K-12 clones. High-speed tracking of single ATCC10798 cells showed forward and backward swimming with an average turning angle of 150°. The flagellar helicity remained right-handed with a 1.3 μm pitch and 0.14 μm helix radius, which is consistent with the feature of a curly type, regardless of motor switching; the flagella of ATCC10798 did not show polymorphic transformation. The torque and rotational switching of the motor was almost identical to the E. coli W3110 strain, which is a derivative of K-12 and a wild-type for chemotaxis. The single point mutation of N87K in FliC, one of the filament subunits, is critical to the change in flagellar morphology and swimming pattern, and lack of flagellar polymorphism. E. coli cells expressing FliC(N87K) sensed ascending a chemotactic gradient in liquid but did not spread on a semi-solid surface. Based on these results, we concluded that a flagellar polymorphism is essential for spreading in structured environments. More... »

PAGES

15887

References to SciGraph publications

  • 1974-05. Flagellar rotation and the mechanism of bacterial motility in NATURE
  • 2013-07-07. Bacteria can exploit a flagellar buckling instability to change direction in NATURE PHYSICS
  • 2016-05-18. Polar flagella rotation in Vibrio parahaemolyticus confers resistance to bacteriophage infection in SCIENTIFIC REPORTS
  • 2017-10-16. A structural model of flagellar filament switching across multiple bacterial species in NATURE COMMUNICATIONS
  • 1973-10. Bacteria Swim by Rotating their Flagellar Filaments in NATURE
  • 2016-08-26. Direct observation of rotation and steps of the archaellum in the swimming halophilic archaeon Halobacterium salinarum in NATURE MICROBIOLOGY
  • 2015-11-02. High-throughput 3D tracking of bacteria on a standard phase contrast microscope in NATURE COMMUNICATIONS
  • 1975-05. Construction of bacterial flagella in NATURE
  • 2017-12-21. Unforeseen swimming and gliding mode of an insect gut symbiont, Burkholderia sp. RPE64, with wrapping of the flagella around its cell body in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2003-08. Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy in NATURE
  • 2018-09-26. Bipolar lophotrichous Helicobacter suis combine extended and wrapped flagella bundles to exhibit multiple modes of motility in SCIENTIFIC REPORTS
  • 2018-07-31. Linear motor driven-rotary motion of a membrane-permeabilized ghost in Mycoplasma mobile in SCIENTIFIC REPORTS
  • 2010-03-14. Conformational change of flagellin for polymorphic supercoiling of the flagellar filament in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2017-12-01. A polar bundle of flagella can drive bacterial swimming by pushing, pulling, or coiling around the cell body in SCIENTIFIC REPORTS
  • 2019-11-06. Chemotaxis as a navigation strategy to boost range expansion in NATURE
  • 2018-12-18. Spatial arrangement of several flagellins within bacterial flagella improves motility in different environments in NATURE COMMUNICATIONS
  • 1972-10. Chemotaxis in Escherichia coli analysed by Three-dimensional Tracking in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1

    DOI

    http://dx.doi.org/10.1038/s41598-020-72429-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1131231896

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32985511


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chemotaxis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli K12", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Flagella", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Models, Biological", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutation", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Molecular Physiology Laboratory, RIKEN, Wako, Japan", 
              "id": "http://www.grid.ac/institutes/grid.7597.c", 
              "name": [
                "Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan", 
                "Department of Physics, University of Oxford, Park load, OX1 3PU, Oxford, UK", 
                "Molecular Physiology Laboratory, RIKEN, Wako, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kinosita", 
            "givenName": "Yoshiaki", 
            "id": "sg:person.016370455126.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016370455126.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan", 
              "id": "http://www.grid.ac/institutes/grid.257114.4", 
              "name": [
                "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ishida", 
            "givenName": "Tsubasa", 
            "id": "sg:person.014240554547.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014240554547.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan", 
              "id": "http://www.grid.ac/institutes/grid.257114.4", 
              "name": [
                "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yoshida", 
            "givenName": "Myu", 
            "id": "sg:person.01037221602.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037221602.77"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan", 
              "id": "http://www.grid.ac/institutes/grid.257114.4", 
              "name": [
                "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ito", 
            "givenName": "Rie", 
            "id": "sg:person.015036135147.61", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015036135147.61"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan", 
              "id": "http://www.grid.ac/institutes/grid.258806.1", 
              "name": [
                "Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Morimoto", 
            "givenName": "Yusuke V.", 
            "id": "sg:person.0727414150.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727414150.29"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan", 
              "id": "http://www.grid.ac/institutes/grid.256169.f", 
              "name": [
                "Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Goto", 
            "givenName": "Kazuki", 
            "id": "sg:person.010235265061.68", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010235265061.68"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physics, University of Oxford, Park load, OX1 3PU, Oxford, UK", 
              "id": "http://www.grid.ac/institutes/grid.4991.5", 
              "name": [
                "Department of Physics, University of Oxford, Park load, OX1 3PU, Oxford, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Berry", 
            "givenName": "Richard M.", 
            "id": "sg:person.01300410534.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300410534.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan", 
              "id": "http://www.grid.ac/institutes/grid.256169.f", 
              "name": [
                "Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nishizaka", 
            "givenName": "Takayuki", 
            "id": "sg:person.01372020626.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372020626.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan", 
              "id": "http://www.grid.ac/institutes/grid.257114.4", 
              "name": [
                "Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sowa", 
            "givenName": "Yoshiyuki", 
            "id": "sg:person.01047733534.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047733534.32"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/s41598-018-32686-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1107129442", 
              "https://doi.org/10.1038/s41598-018-32686-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/239500a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028194584", 
              "https://doi.org/10.1038/239500a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature01830", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000565071", 
              "https://doi.org/10.1038/nature01830"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/249073a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013527060", 
              "https://doi.org/10.1038/249073a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-018-29875-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105832167", 
              "https://doi.org/10.1038/s41598-018-29875-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmicrobiol.2016.148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008438133", 
              "https://doi.org/10.1038/nmicrobiol.2016.148"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-16428-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1093031902", 
              "https://doi.org/10.1038/s41598-017-16428-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41396-017-0010-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1099738979", 
              "https://doi.org/10.1038/s41396-017-0010-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41586-019-1733-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1122331185", 
              "https://doi.org/10.1038/s41586-019-1733-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41467-018-07802-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110559604", 
              "https://doi.org/10.1038/s41467-018-07802-w"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/255121a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016034428", 
              "https://doi.org/10.1038/255121a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41467-017-01075-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092152763", 
              "https://doi.org/10.1038/s41467-017-01075-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1774", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000526904", 
              "https://doi.org/10.1038/nsmb.1774"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep26147", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001227801", 
              "https://doi.org/10.1038/srep26147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nphys2676", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037938190", 
              "https://doi.org/10.1038/nphys2676"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms9776", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015996270", 
              "https://doi.org/10.1038/ncomms9776"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/245380a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006993053", 
              "https://doi.org/10.1038/245380a0"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2020-09-28", 
        "datePublishedReg": "2020-09-28", 
        "description": "Most motile bacteria are propelled by rigid, helical, flagellar filaments and display distinct swimming patterns to explore their favorable environments. Escherichia coli cells have a reversible rotary motor at the base of each filament. They exhibit a run-tumble swimming pattern, driven by switching of the rotational direction, which causes polymorphic flagellar transformation. Here we report a novel swimming mode in E. coli ATCC10798, which is one of the original K-12 clones. High-speed tracking of single ATCC10798 cells showed forward and backward swimming with an average turning angle of 150\u00b0. The flagellar helicity remained right-handed with a 1.3\u00a0\u03bcm pitch and 0.14\u00a0\u03bcm helix radius, which is consistent with the\u00a0feature of a curly type, regardless of motor switching; the flagella of ATCC10798 did not show polymorphic transformation. The torque and rotational switching of the motor was almost identical to the E. coli W3110 strain, which is a derivative of K-12 and a wild-type for chemotaxis. The single point mutation of N87K in FliC, one of the filament subunits, is critical to the change in flagellar morphology and swimming pattern, and lack of flagellar polymorphism. E. coli cells expressing FliC(N87K) sensed ascending a chemotactic gradient in liquid but did not spread on a semi-solid surface. Based on these results, we concluded that a flagellar polymorphism is essential for spreading in structured environments.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/s41598-020-72429-1", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.7522024", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.4576491", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.9454389", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5857619", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5863309", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5871626", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.7532989", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.9197552", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.8430349", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "10"
          }
        ], 
        "keywords": [
          "distinct chemotactic behavior", 
          "N87K", 
          "chemotactic gradient", 
          "distinct swimming patterns", 
          "cells", 
          "point mutations", 
          "flagellar morphology", 
          "polymorphism", 
          "swimming patterns", 
          "single point mutation", 
          "chemotaxis", 
          "curly types", 
          "patterns", 
          "chemotactic behavior", 
          "mutations", 
          "Most motile bacteria", 
          "reversible rotary motor", 
          "W3110 strain", 
          "FliC", 
          "clones", 
          "lack", 
          "swimming", 
          "backward swimming", 
          "strains", 
          "motor", 
          "changes", 
          "bacteria", 
          "subunits", 
          "motile bacteria", 
          "favorable environment", 
          "coli cells", 
          "E. coli cells", 
          "filament subunits", 
          "coli K", 
          "types", 
          "Escherichia coli K", 
          "flagellar filaments", 
          "features", 
          "movement", 
          "results", 
          "filaments", 
          "flagellar polymorphism", 
          "Escherichia coli cells", 
          "flagella", 
          "derivatives", 
          "base", 
          "morphology", 
          "motor switching", 
          "semi-solid surfaces", 
          "behavior", 
          "flagellar transformation", 
          "environment", 
          "rotational direction", 
          "gradient", 
          "torque", 
          "transformation", 
          "angle", 
          "direction", 
          "mode", 
          "surface", 
          "tracking", 
          "swimming modes", 
          "pitch", 
          "structured environment", 
          "switching", 
          "radius", 
          "liquid", 
          "high-speed tracking", 
          "rotary motor", 
          "rotational switching", 
          "helicity", 
          "polymorphic transformation", 
          "helix radius"
        ], 
        "name": "Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements", 
        "pagination": "15887", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1131231896"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-020-72429-1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "32985511"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-020-72429-1", 
          "https://app.dimensions.ai/details/publication/pub.1131231896"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-05-20T07:36", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_841.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/s41598-020-72429-1"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-020-72429-1'


     

    This table displays all metadata directly associated to this object as RDF triples.

    310 TRIPLES      22 PREDICATES      121 URIs      96 LITERALS      12 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-020-72429-1 schema:about N5d2a127c78684c69902450d695de9da4
    2 N6c1a7dd88f1c42238a66be196d9a320a
    3 N75564df295a849cab2d77d799bb9d3f4
    4 Na6455a3b18284d4ea468792fc351ccd5
    5 Nf01df1a1a41b4cf5a7b8450a5a7b3f16
    6 anzsrc-for:06
    7 anzsrc-for:0601
    8 schema:author N47cc1bece5da48fc856d3f9a3e800443
    9 schema:citation sg:pub.10.1038/239500a0
    10 sg:pub.10.1038/245380a0
    11 sg:pub.10.1038/249073a0
    12 sg:pub.10.1038/255121a0
    13 sg:pub.10.1038/nature01830
    14 sg:pub.10.1038/ncomms9776
    15 sg:pub.10.1038/nmicrobiol.2016.148
    16 sg:pub.10.1038/nphys2676
    17 sg:pub.10.1038/nsmb.1774
    18 sg:pub.10.1038/s41396-017-0010-z
    19 sg:pub.10.1038/s41467-017-01075-5
    20 sg:pub.10.1038/s41467-018-07802-w
    21 sg:pub.10.1038/s41586-019-1733-y
    22 sg:pub.10.1038/s41598-017-16428-9
    23 sg:pub.10.1038/s41598-018-29875-9
    24 sg:pub.10.1038/s41598-018-32686-7
    25 sg:pub.10.1038/srep26147
    26 schema:datePublished 2020-09-28
    27 schema:datePublishedReg 2020-09-28
    28 schema:description Most motile bacteria are propelled by rigid, helical, flagellar filaments and display distinct swimming patterns to explore their favorable environments. Escherichia coli cells have a reversible rotary motor at the base of each filament. They exhibit a run-tumble swimming pattern, driven by switching of the rotational direction, which causes polymorphic flagellar transformation. Here we report a novel swimming mode in E. coli ATCC10798, which is one of the original K-12 clones. High-speed tracking of single ATCC10798 cells showed forward and backward swimming with an average turning angle of 150°. The flagellar helicity remained right-handed with a 1.3 μm pitch and 0.14 μm helix radius, which is consistent with the feature of a curly type, regardless of motor switching; the flagella of ATCC10798 did not show polymorphic transformation. The torque and rotational switching of the motor was almost identical to the E. coli W3110 strain, which is a derivative of K-12 and a wild-type for chemotaxis. The single point mutation of N87K in FliC, one of the filament subunits, is critical to the change in flagellar morphology and swimming pattern, and lack of flagellar polymorphism. E. coli cells expressing FliC(N87K) sensed ascending a chemotactic gradient in liquid but did not spread on a semi-solid surface. Based on these results, we concluded that a flagellar polymorphism is essential for spreading in structured environments.
    29 schema:genre article
    30 schema:inLanguage en
    31 schema:isAccessibleForFree true
    32 schema:isPartOf N4ab6e03187484f0d9b6da9b475d57e29
    33 N678898b1631648e9a4f81d134b457384
    34 sg:journal.1045337
    35 schema:keywords E. coli cells
    36 Escherichia coli K
    37 Escherichia coli cells
    38 FliC
    39 Most motile bacteria
    40 N87K
    41 W3110 strain
    42 angle
    43 backward swimming
    44 bacteria
    45 base
    46 behavior
    47 cells
    48 changes
    49 chemotactic behavior
    50 chemotactic gradient
    51 chemotaxis
    52 clones
    53 coli K
    54 coli cells
    55 curly types
    56 derivatives
    57 direction
    58 distinct chemotactic behavior
    59 distinct swimming patterns
    60 environment
    61 favorable environment
    62 features
    63 filament subunits
    64 filaments
    65 flagella
    66 flagellar filaments
    67 flagellar morphology
    68 flagellar polymorphism
    69 flagellar transformation
    70 gradient
    71 helicity
    72 helix radius
    73 high-speed tracking
    74 lack
    75 liquid
    76 mode
    77 morphology
    78 motile bacteria
    79 motor
    80 motor switching
    81 movement
    82 mutations
    83 patterns
    84 pitch
    85 point mutations
    86 polymorphic transformation
    87 polymorphism
    88 radius
    89 results
    90 reversible rotary motor
    91 rotary motor
    92 rotational direction
    93 rotational switching
    94 semi-solid surfaces
    95 single point mutation
    96 strains
    97 structured environment
    98 subunits
    99 surface
    100 swimming
    101 swimming modes
    102 swimming patterns
    103 switching
    104 torque
    105 tracking
    106 transformation
    107 types
    108 schema:name Distinct chemotactic behavior in the original Escherichia coli K-12 depending on forward-and-backward swimming, not on run-tumble movements
    109 schema:pagination 15887
    110 schema:productId N050ba6a2e195431cb8ab3b0096d9cc67
    111 Nb1ca3d776d8c44219e26ed1361755369
    112 Nef7f3a9c33ef43d7810dc353a8ed18b7
    113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1131231896
    114 https://doi.org/10.1038/s41598-020-72429-1
    115 schema:sdDatePublished 2022-05-20T07:36
    116 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    117 schema:sdPublisher N61ffc7a1c3194357bce8f3bc987cfd11
    118 schema:url https://doi.org/10.1038/s41598-020-72429-1
    119 sgo:license sg:explorer/license/
    120 sgo:sdDataset articles
    121 rdf:type schema:ScholarlyArticle
    122 N050ba6a2e195431cb8ab3b0096d9cc67 schema:name dimensions_id
    123 schema:value pub.1131231896
    124 rdf:type schema:PropertyValue
    125 N40e1b200db4f4229b4880d799051b6f0 rdf:first sg:person.0727414150.29
    126 rdf:rest Ne198e1f617154f3986ca70ade53da2a8
    127 N40eca2b96f5a46b3bc7c03705fc82e24 rdf:first sg:person.014240554547.01
    128 rdf:rest N808b4fdb70864162ad9e17ba96052804
    129 N47cc1bece5da48fc856d3f9a3e800443 rdf:first sg:person.016370455126.53
    130 rdf:rest N40eca2b96f5a46b3bc7c03705fc82e24
    131 N4ab6e03187484f0d9b6da9b475d57e29 schema:issueNumber 1
    132 rdf:type schema:PublicationIssue
    133 N5d2a127c78684c69902450d695de9da4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Chemotaxis
    135 rdf:type schema:DefinedTerm
    136 N61ffc7a1c3194357bce8f3bc987cfd11 schema:name Springer Nature - SN SciGraph project
    137 rdf:type schema:Organization
    138 N678898b1631648e9a4f81d134b457384 schema:volumeNumber 10
    139 rdf:type schema:PublicationVolume
    140 N6c1a7dd88f1c42238a66be196d9a320a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Flagella
    142 rdf:type schema:DefinedTerm
    143 N75564df295a849cab2d77d799bb9d3f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Models, Biological
    145 rdf:type schema:DefinedTerm
    146 N7e73cd93129d470dad3c6c0e6482ac9f rdf:first sg:person.01372020626.32
    147 rdf:rest Nb1270941a7cb4bcda3b38703a505f567
    148 N808b4fdb70864162ad9e17ba96052804 rdf:first sg:person.01037221602.77
    149 rdf:rest N87492051a3de4de08f94f507721e7144
    150 N87492051a3de4de08f94f507721e7144 rdf:first sg:person.015036135147.61
    151 rdf:rest N40e1b200db4f4229b4880d799051b6f0
    152 Na6455a3b18284d4ea468792fc351ccd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Escherichia coli K12
    154 rdf:type schema:DefinedTerm
    155 Nb1270941a7cb4bcda3b38703a505f567 rdf:first sg:person.01047733534.32
    156 rdf:rest rdf:nil
    157 Nb1ca3d776d8c44219e26ed1361755369 schema:name pubmed_id
    158 schema:value 32985511
    159 rdf:type schema:PropertyValue
    160 Nde04686340f4440195027479cca60c3c rdf:first sg:person.01300410534.17
    161 rdf:rest N7e73cd93129d470dad3c6c0e6482ac9f
    162 Ne198e1f617154f3986ca70ade53da2a8 rdf:first sg:person.010235265061.68
    163 rdf:rest Nde04686340f4440195027479cca60c3c
    164 Nef7f3a9c33ef43d7810dc353a8ed18b7 schema:name doi
    165 schema:value 10.1038/s41598-020-72429-1
    166 rdf:type schema:PropertyValue
    167 Nf01df1a1a41b4cf5a7b8450a5a7b3f16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Mutation
    169 rdf:type schema:DefinedTerm
    170 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    171 schema:name Biological Sciences
    172 rdf:type schema:DefinedTerm
    173 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    174 schema:name Biochemistry and Cell Biology
    175 rdf:type schema:DefinedTerm
    176 sg:grant.4576491 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    177 rdf:type schema:MonetaryGrant
    178 sg:grant.5857619 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    179 rdf:type schema:MonetaryGrant
    180 sg:grant.5863309 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    181 rdf:type schema:MonetaryGrant
    182 sg:grant.5871626 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    183 rdf:type schema:MonetaryGrant
    184 sg:grant.7522024 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    185 rdf:type schema:MonetaryGrant
    186 sg:grant.7532989 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    187 rdf:type schema:MonetaryGrant
    188 sg:grant.8430349 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    189 rdf:type schema:MonetaryGrant
    190 sg:grant.9197552 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    191 rdf:type schema:MonetaryGrant
    192 sg:grant.9454389 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-020-72429-1
    193 rdf:type schema:MonetaryGrant
    194 sg:journal.1045337 schema:issn 2045-2322
    195 schema:name Scientific Reports
    196 schema:publisher Springer Nature
    197 rdf:type schema:Periodical
    198 sg:person.010235265061.68 schema:affiliation grid-institutes:grid.256169.f
    199 schema:familyName Goto
    200 schema:givenName Kazuki
    201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010235265061.68
    202 rdf:type schema:Person
    203 sg:person.01037221602.77 schema:affiliation grid-institutes:grid.257114.4
    204 schema:familyName Yoshida
    205 schema:givenName Myu
    206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037221602.77
    207 rdf:type schema:Person
    208 sg:person.01047733534.32 schema:affiliation grid-institutes:grid.257114.4
    209 schema:familyName Sowa
    210 schema:givenName Yoshiyuki
    211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047733534.32
    212 rdf:type schema:Person
    213 sg:person.01300410534.17 schema:affiliation grid-institutes:grid.4991.5
    214 schema:familyName Berry
    215 schema:givenName Richard M.
    216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300410534.17
    217 rdf:type schema:Person
    218 sg:person.01372020626.32 schema:affiliation grid-institutes:grid.256169.f
    219 schema:familyName Nishizaka
    220 schema:givenName Takayuki
    221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372020626.32
    222 rdf:type schema:Person
    223 sg:person.014240554547.01 schema:affiliation grid-institutes:grid.257114.4
    224 schema:familyName Ishida
    225 schema:givenName Tsubasa
    226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014240554547.01
    227 rdf:type schema:Person
    228 sg:person.015036135147.61 schema:affiliation grid-institutes:grid.257114.4
    229 schema:familyName Ito
    230 schema:givenName Rie
    231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015036135147.61
    232 rdf:type schema:Person
    233 sg:person.016370455126.53 schema:affiliation grid-institutes:grid.7597.c
    234 schema:familyName Kinosita
    235 schema:givenName Yoshiaki
    236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016370455126.53
    237 rdf:type schema:Person
    238 sg:person.0727414150.29 schema:affiliation grid-institutes:grid.258806.1
    239 schema:familyName Morimoto
    240 schema:givenName Yusuke V.
    241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727414150.29
    242 rdf:type schema:Person
    243 sg:pub.10.1038/239500a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028194584
    244 https://doi.org/10.1038/239500a0
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1038/245380a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006993053
    247 https://doi.org/10.1038/245380a0
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1038/249073a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013527060
    250 https://doi.org/10.1038/249073a0
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/255121a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016034428
    253 https://doi.org/10.1038/255121a0
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/nature01830 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000565071
    256 https://doi.org/10.1038/nature01830
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/ncomms9776 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015996270
    259 https://doi.org/10.1038/ncomms9776
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/nmicrobiol.2016.148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008438133
    262 https://doi.org/10.1038/nmicrobiol.2016.148
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1038/nphys2676 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037938190
    265 https://doi.org/10.1038/nphys2676
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/nsmb.1774 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000526904
    268 https://doi.org/10.1038/nsmb.1774
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/s41396-017-0010-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1099738979
    271 https://doi.org/10.1038/s41396-017-0010-z
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1038/s41467-017-01075-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092152763
    274 https://doi.org/10.1038/s41467-017-01075-5
    275 rdf:type schema:CreativeWork
    276 sg:pub.10.1038/s41467-018-07802-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1110559604
    277 https://doi.org/10.1038/s41467-018-07802-w
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1038/s41586-019-1733-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1122331185
    280 https://doi.org/10.1038/s41586-019-1733-y
    281 rdf:type schema:CreativeWork
    282 sg:pub.10.1038/s41598-017-16428-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1093031902
    283 https://doi.org/10.1038/s41598-017-16428-9
    284 rdf:type schema:CreativeWork
    285 sg:pub.10.1038/s41598-018-29875-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105832167
    286 https://doi.org/10.1038/s41598-018-29875-9
    287 rdf:type schema:CreativeWork
    288 sg:pub.10.1038/s41598-018-32686-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107129442
    289 https://doi.org/10.1038/s41598-018-32686-7
    290 rdf:type schema:CreativeWork
    291 sg:pub.10.1038/srep26147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001227801
    292 https://doi.org/10.1038/srep26147
    293 rdf:type schema:CreativeWork
    294 grid-institutes:grid.256169.f schema:alternateName Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan
    295 schema:name Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan
    296 rdf:type schema:Organization
    297 grid-institutes:grid.257114.4 schema:alternateName Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan
    298 schema:name Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University, 184-8584, Tokyo, Japan
    299 rdf:type schema:Organization
    300 grid-institutes:grid.258806.1 schema:alternateName Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan
    301 schema:name Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan
    302 rdf:type schema:Organization
    303 grid-institutes:grid.4991.5 schema:alternateName Department of Physics, University of Oxford, Park load, OX1 3PU, Oxford, UK
    304 schema:name Department of Physics, University of Oxford, Park load, OX1 3PU, Oxford, UK
    305 rdf:type schema:Organization
    306 grid-institutes:grid.7597.c schema:alternateName Molecular Physiology Laboratory, RIKEN, Wako, Japan
    307 schema:name Department of Physics, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, 171-8588, Tokyo, Japan
    308 Department of Physics, University of Oxford, Park load, OX1 3PU, Oxford, UK
    309 Molecular Physiology Laboratory, RIKEN, Wako, Japan
    310 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...