Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-11-19

AUTHORS

Neil T. Sullivan, Will Dampier, Cheng-Han Chung, Alexander G. Allen, Andrew Atkins, Vanessa Pirrone, Greg Homan, Shendra Passic, Jean Williams, Wen Zhong, Katherine Kercher, Mathew Desimone, Luna Li, Gregory C. Antell, Joshua Chang Mell, Garth D. Ehrlich, Zsofia Szep, Jeffrey M. Jacobson, Michael R. Nonnemacher, Brian Wigdahl

ABSTRACT

The CRISPR/Cas9 system has been proposed as a cure strategy for HIV. However, few published guide RNAs (gRNAs) are predicted to cleave the majority of HIV-1 viral quasispecies (vQS) observed within and among patients. We report the design of a novel pipeline to identify gRNAs that target HIV across a large number of infected individuals. Next generation sequencing (NGS) of LTRs from 269 HIV-1-infected samples in the Drexel CARES Cohort was used to select gRNAs with predicted broad-spectrum activity. In silico, D-LTR-P4-227913 (package of the top 4 gRNAs) accounted for all detectable genetic variation within the vQS of the 269 samples and the Los Alamos National Laboratory HIV database. In silico secondary structure analyses from NGS indicated extensive TAR stem-loop malformations predicted to inactivate proviral transcription, which was confirmed by reduced viral gene expression in TZM-bl or P4R5 cells. Similarly, a high sensitivity in vitro CRISPR/Cas9 cleavage assay showed that the top-ranked gRNA was the most effective at cleaving patient-derived HIV-1 LTRs from five patients. Furthermore, the D-LTR-P4-227913 was predicted to cleave a median of 96.1% of patient-derived sequences from other HIV subtypes. These results demonstrate that the gRNAs possess broad-spectrum cutting activity and could contribute to an HIV cure. More... »

PAGES

17088

References to SciGraph publications

  • 2016-07-12. Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9 in SCIENTIFIC REPORTS
  • 2016-04-07. Lentivirus pre-packed with Cas9 protein for safer gene editing in GENE THERAPY
  • 2017-10-31. Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants in SCIENTIFIC REPORTS
  • 2017-04-17. HIV persistence in tissue macrophages of humanized myeloid-only mice during antiretroviral therapy in NATURE MEDICINE
  • 2015-11-05. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs in SCIENTIFIC REPORTS
  • 2016-05-12. Exosome-associated release, uptake, and neurotoxicity of HIV-1 Tat protein in JOURNAL OF NEUROVIROLOGY
  • 1997-05. Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection in NATURE
  • 2018-07-11. Viral diversity is an obligate consideration in CRISPR/Cas9 designs for targeting the HIV reservoir in BMC BIOLOGY
  • 2016-05-19. Excision of HIV-1 DNA by gene editing: a proof-of-concept in vivo study in GENE THERAPY
  • 2017-09-09. Genome editing of the HIV co-receptors CCR5 and CXCR4 by CRISPR-Cas9 protects CD4+ T cells from HIV-1 infection in CELL & BIOSCIENCE
  • 2017-05-01. CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets in NATURE METHODS
  • 2017-07-21. Suppression of HBV replication by the expression of nickase- and nuclease dead-Cas9 in SCIENTIFIC REPORTS
  • 2011-01-12. Development of co-selected single nucleotide polymorphisms in the viral promoter precedes the onset of human immunodeficiency virus type 1-associated neurocognitive impairment in JOURNAL OF NEUROVIROLOGY
  • 2016-08-16. Negative Feedback Regulation of HIV-1 by Gene Editing Strategy in SCIENTIFIC REPORTS
  • 2017-07-20. A CRISPR/Cas9 guidance RNA screen platform for HIV provirus disruption and HIV/AIDS gene therapy in astrocytes in SCIENTIFIC REPORTS
  • 2017-06-01. Current application of CRISPR/Cas9 gene-editing technique to eradication of HIV/AIDS in GENE THERAPY
  • 2014-12-16. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases in NATURE BIOTECHNOLOGY
  • 1999-05-01. Latent infection of CD4+ T cells provides a mechanism for lifelong persistence of HIV-1, even in patients on effective combination therapy in NATURE MEDICINE
  • 2016-10-04. The variances of Sp1 and NF-κB elements correlate with the greater capacity of Chinese HIV-1 B′-LTR for driving gene expression in SCIENTIFIC REPORTS
  • 2015-03-10. Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells in NATURE COMMUNICATIONS
  • 2016-05-03. Utilization of HIV-1 envelope V3 to identify X4- and R5-specific Tat and LTR sequence signatures in RETROVIROLOGY
  • 2013-08-26. Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus in SCIENTIFIC REPORTS
  • 2013-07-21. DNA targeting specificity of RNA-guided Cas9 nucleases in NATURE BIOTECHNOLOGY
  • 2015-10-20. Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection in SCIENTIFIC REPORTS
  • 2017-07-31. Implications of human genetic variation in CRISPR-based therapeutic genome editing in NATURE MEDICINE
  • 2017-11-15. Genome modification of CXCR4 by Staphylococcus aureus Cas9 renders cells resistance to HIV-1 infection in RETROVIROLOGY
  • 2016-01-18. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 in NATURE BIOTECHNOLOGY
  • 2017-02-08. A combinational CRISPR/Cas9 gene-editing approach can halt HIV replication and prevent viral escape in SCIENTIFIC REPORTS
  • 2016-03-04. Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing in SCIENTIFIC REPORTS
  • 2015-02-27. The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA in RETROVIROLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-52353-9

    DOI

    http://dx.doi.org/10.1038/s41598-019-52353-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1122693634

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/31745112


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