Identification of key genes involved in the biosynthesis of triterpenic acids in the mint family View Full Text


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Article Info

DATE

2019-11-01

AUTHORS

Zahra Aminfar, Babak Rabiei, Masoud Tohidfar, Mohammad Hossein Mirjalili

ABSTRACT

Triterpenic acids (TAs), a large group of natural compounds with diverse biological activity, are produced by several plant taxa. Betulinic, oleanolic, and ursolic acids are the most medicinally important TAs and are mainly found in plants of the mint family. Metabolic engineering is strongly dependent on identifying the key genes in biosynthetic pathways toward the products of interest. In this study, gene expression tracking was performed by transcriptome mining, co-expression network analysis, and tissue-specific metabolite-expression analysis in order to identify possible key genes involved in TAs biosynthetic pathways. To this end, taxa-specific degenerate primers of six important genes were designed using an effective method based on the MEME algorithm in a phylogenetically related group of sequences and successfully applied in three members of the Lamiaceae (Rosmarinus officinalis, Salvia officinalis, and Thymus persicus). Based on the results of in-depth data analysis, genes encoding squalene epoxidase and oxido squalene cyclases are proposed as targets for boosting triterpene production. The results emphasize the importance of identifying key genes in triterpene biosynthesis, which may facilitate genetic manipulation or overexpression of target genes. More... »

PAGES

15826

References to SciGraph publications

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  • 2010-07-23. Simultaneous determination and quantification of three pentacyclic triterpenoids—betulinic acid, oleanolic acid, and ursolic acid—in cell cultures of Lantana camara L. in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2011-05-15. Full-length transcriptome assembly from RNA-Seq data without a reference genome in NATURE BIOTECHNOLOGY
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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-52090-z

    DOI

    http://dx.doi.org/10.1038/s41598-019-52090-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1122258634

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/31676750


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    76 network analysis
    77 order
    78 overexpression
    79 pathway
    80 plant taxa
    81 plants
    82 possible key genes
    83 primers
    84 product of interest
    85 production
    86 products
    87 results
    88 sequence
    89 squalene cyclases
    90 squalene epoxidase
    91 study
    92 target
    93 target genes
    94 taxa
    95 tracking
    96 transcriptome mining
    97 triterpene biosynthesis
    98 triterpene production
    99 triterpenic acids
    100 ursolic acid
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