Detailed Evaluation of Possible Ganglion Cell Loss in the Retina of Zucker Diabetic Fatty (ZDF) Rats View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-07-18

AUTHORS

Rozina I. Hajdú, Lenke K. Laurik, Klaudia Szabó, Bulcsú Dékány, Zsuzsanna Almási, Anna Énzsöly, Arnold Szabó, Tamás Radovits, Csaba Mátyás, Attila Oláh, Ágoston Szél, Gábor M. Somfai, Csaba Dávid, Ákos Lukáts

ABSTRACT

A thinning of the inner retina is one of the earliest potential markers of neuroretinal damage in diabetic subjects. The histological background is uncertain; retinal ganglion cell (RGC) loss and changes in the structure or thickness of the inner plexiform layer (IPL) have been suspected. Studies conducted on animal models on RGC pathology gave contradictory results. Hereby we present RGC numbers, distribution patterns and IPL thickness from Zucker Diabetic Fatty (ZDF) rats. After labelling RGCs on retinal whole mounts, isodensity maps were constructed, RGC numbers and distribution patterns analysed using a custom-built algorithm, enabling point-by-point comparison. There was no change in staining characteristics of the antibodies and no significant difference in average RGC densities was found compared to controls. The distribution patterns were also comparable and no significant difference was found in IPL thickness and stratification or in the number of apoptotic cells in the ganglion cell layer (GCL). Our results provide a detailed evaluation of the inner retina and exclude major RGC loss in ZDF rats and suggest that other factors could serve as a potential explanation for inner retinal thinning in clinical studies. Our custom-built method could be adopted for the assessment of other animal or human retinas. More... »

PAGES

10463

References to SciGraph publications

  • 2012-06-28. Fiji: an open-source platform for biological-image analysis in NATURE METHODS
  • 2003-07. Expression pattern of Glycoconjugates in Rat Retina as Analysed by Lectin Histochemistry in JOURNAL OF MOLECULAR HISTOLOGY
  • 2017-01-26. Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness in SCIENTIFIC REPORTS
  • 2017-09-24. Diabetic retinopathy: hyperglycaemia, oxidative stress and beyond in DIABETOLOGIA
  • 2013-05-03. Regulation and effects of GDF-15 in the retina following optic nerve crush in CELL AND TISSUE RESEARCH
  • 2014-11-20. Stratified organization and disorganization of inner plexiform layer revealed by TNAP activity in healthy and diabetic rat retina in CELL AND TISSUE RESEARCH
  • 2018-01-19. Animal models of obesity and diabetes mellitus in NATURE REVIEWS ENDOCRINOLOGY
  • 2001-03. Vertical interactions across ten parallel, stacked representations in the mammalian retina in NATURE
  • 2016-10-20. Presence and Risk Factors for Glaucoma in Patients with Diabetes in CURRENT DIABETES REPORTS
  • 2003-07-31. Apoptotic death of photoreceptors in the streptozotocin-induced diabetic rat retina in DIABETOLOGIA
  • 2017-03-03. Structure–function relationship in early diabetic retinopathy: a spatial correlation analysis with OCT and microperimetry in EYE
  • 2004-07-01. Analyzing Markers of Apoptosis In Vitro in CHECKPOINT CONTROLS AND CANCER
  • 2017-08-21. Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats in SCIENTIFIC REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-46879-1

    DOI

    http://dx.doi.org/10.1038/s41598-019-46879-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1118092357

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/31320684


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Clinical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Apoptosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blood Glucose", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Body Weight", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Diabetes Mellitus, Experimental", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Optic Nerve", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Rats", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Rats, Zucker", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Retinal Ganglion Cells", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Ophthalmology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
                "Department of Ophthalmology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hajd\u00fa", 
            "givenName": "Rozina I.", 
            "id": "sg:person.012216010747.36", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012216010747.36"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Ophthalmology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Ophthalmology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Laurik", 
            "givenName": "Lenke K.", 
            "id": "sg:person.0631267044.97", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631267044.97"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Szab\u00f3", 
            "givenName": "Klaudia", 
            "id": "sg:person.01346751160.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346751160.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "D\u00e9k\u00e1ny", 
            "givenName": "Bulcs\u00fa", 
            "id": "sg:person.010025467347.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010025467347.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Alm\u00e1si", 
            "givenName": "Zsuzsanna", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Ophthalmology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
                "Department of Ophthalmology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "\u00c9nzs\u00f6ly", 
            "givenName": "Anna", 
            "id": "sg:person.01010512720.51", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010512720.51"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Szab\u00f3", 
            "givenName": "Arnold", 
            "id": "sg:person.014543626542.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014543626542.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heart and Vascular Center, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Heart and Vascular Center, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Radovits", 
            "givenName": "Tam\u00e1s", 
            "id": "sg:person.01332471212.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332471212.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heart and Vascular Center, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Heart and Vascular Center, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "M\u00e1ty\u00e1s", 
            "givenName": "Csaba", 
            "id": "sg:person.01063426430.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063426430.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Heart and Vascular Center, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Heart and Vascular Center, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ol\u00e1h", 
            "givenName": "Attila", 
            "id": "sg:person.013565440475.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013565440475.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sz\u00e9l", 
            "givenName": "\u00c1goston", 
            "id": "sg:person.0576734502.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576734502.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Retinology Unit, Pallas Kliniken, Olten, Switzerland", 
              "id": "http://www.grid.ac/institutes/None", 
              "name": [
                "Department of Ophthalmology, Semmelweis University, Budapest, Hungary", 
                "Retinology Unit, Pallas Kliniken, Olten, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Somfai", 
            "givenName": "G\u00e1bor M.", 
            "id": "sg:person.0617553001.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617553001.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "D\u00e1vid", 
            "givenName": "Csaba", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary", 
              "id": "http://www.grid.ac/institutes/grid.11804.3c", 
              "name": [
                "Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Luk\u00e1ts", 
            "givenName": "\u00c1kos", 
            "id": "sg:person.0723452446.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723452446.75"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nmeth.2019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026706357", 
              "https://doi.org/10.1038/nmeth.2019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11892-016-0815-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026116413", 
              "https://doi.org/10.1007/s11892-016-0815-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-09068-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091255902", 
              "https://doi.org/10.1038/s41598-017-09068-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1385/1-59259-811-0:313", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018267824", 
              "https://doi.org/10.1385/1-59259-811-0:313"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1026032005521", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012967748", 
              "https://doi.org/10.1023/a:1026032005521"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00441-013-1634-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041167220", 
              "https://doi.org/10.1007/s00441-013-1634-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00125-017-4435-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091909657", 
              "https://doi.org/10.1007/s00125-017-4435-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-016-0028-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079403223", 
              "https://doi.org/10.1038/s41598-016-0028-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrendo.2017.161", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100474408", 
              "https://doi.org/10.1038/nrendo.2017.161"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00441-014-2047-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034140930", 
              "https://doi.org/10.1007/s00441-014-2047-x"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35069068", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004850591", 
              "https://doi.org/10.1038/35069068"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/eye.2017.27", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084128034", 
              "https://doi.org/10.1038/eye.2017.27"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00125-003-1177-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051885969", 
              "https://doi.org/10.1007/s00125-003-1177-6"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-07-18", 
        "datePublishedReg": "2019-07-18", 
        "description": "A thinning of the inner retina is one of the earliest potential markers of neuroretinal damage in diabetic subjects. The histological background is uncertain; retinal ganglion cell (RGC) loss and changes in the structure or thickness of the inner plexiform layer (IPL) have been suspected. Studies conducted on animal models on RGC pathology gave contradictory results. Hereby we present RGC numbers, distribution patterns and IPL thickness from Zucker Diabetic Fatty (ZDF) rats. After labelling RGCs on retinal whole mounts, isodensity maps were constructed, RGC numbers and distribution patterns analysed using a custom-built algorithm, enabling point-by-point comparison. There was no change in staining characteristics of the antibodies and no significant difference in average RGC densities was found compared to controls. The distribution patterns were also comparable and no significant difference was found in IPL thickness and stratification or in the number of apoptotic cells in the ganglion cell layer (GCL). Our results provide a detailed evaluation of the inner retina and exclude major RGC loss in ZDF rats and suggest that other factors could serve as a potential explanation for inner retinal thinning in clinical studies. Our custom-built method could be adopted for the assessment of other animal or human retinas.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/s41598-019-46879-1", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.4188749", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "keywords": [
          "Zucker diabetic fatty", 
          "ganglion cell loss", 
          "inner plexiform layer", 
          "ganglion cell layer", 
          "RGC number", 
          "IPL thickness", 
          "inner retina", 
          "cell loss", 
          "retinal ganglion cell loss", 
          "inner retinal thinning", 
          "retinal whole mounts", 
          "significant differences", 
          "neuroretinal damage", 
          "RGC pathology", 
          "retinal thinning", 
          "RGC loss", 
          "RGC density", 
          "diabetic subjects", 
          "ZDF rats", 
          "plexiform layer", 
          "clinical studies", 
          "animal models", 
          "histological background", 
          "potential marker", 
          "human retina", 
          "retina", 
          "apoptotic cells", 
          "cell layer", 
          "detailed evaluation", 
          "whole mounts", 
          "fatty", 
          "contradictory results", 
          "isodensity maps", 
          "rats", 
          "pathology", 
          "distribution patterns", 
          "RGCs", 
          "potential explanation", 
          "antibodies", 
          "differences", 
          "loss", 
          "evaluation", 
          "study", 
          "markers", 
          "subjects", 
          "animals", 
          "cells", 
          "patterns", 
          "changes", 
          "stratification", 
          "damage", 
          "number", 
          "assessment", 
          "thinning", 
          "factors", 
          "control", 
          "background", 
          "results", 
          "mount", 
          "point comparison", 
          "comparison", 
          "thickness", 
          "characteristics", 
          "method", 
          "point", 
          "model", 
          "explanation", 
          "density", 
          "layer", 
          "maps", 
          "structure", 
          "algorithm", 
          "earliest potential markers", 
          "Diabetic Fatty", 
          "average RGC densities", 
          "major RGC loss", 
          "custom-built method", 
          "Possible Ganglion Cell Loss"
        ], 
        "name": "Detailed Evaluation of Possible Ganglion Cell Loss in the Retina of Zucker Diabetic Fatty (ZDF) Rats", 
        "pagination": "10463", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1118092357"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-019-46879-1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "31320684"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-019-46879-1", 
          "https://app.dimensions.ai/details/publication/pub.1118092357"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-01-01T18:54", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_819.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/s41598-019-46879-1"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-46879-1'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-46879-1'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-46879-1'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-46879-1'


     

    This table displays all metadata directly associated to this object as RDF triples.

    330 TRIPLES      22 PREDICATES      127 URIs      106 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-019-46879-1 schema:about N14df0a0e6c614e9991b86c8722d653c8
    2 N4b862f1563b9426681c3b35ca8d0c1ca
    3 N72cd7defa17e4aefad739ea6b4996159
    4 N7650b4b9bfa54d8884108dc876734333
    5 N80989cc342db4d5c958199e3c39c8e8a
    6 N83640e5ecc81476ba921afa8cb5aea86
    7 Nab965cf116574aad964b8669075f673d
    8 Nba06edbc02414486b53b5e90778b5e55
    9 Nd3f4924b7805409cbaa408d7a7c128d1
    10 Nfed0b7758496464d82783c7a60c444cc
    11 anzsrc-for:11
    12 anzsrc-for:1103
    13 schema:author N405aaecc0cb34a2780e36e75dabf2aa9
    14 schema:citation sg:pub.10.1007/s00125-003-1177-6
    15 sg:pub.10.1007/s00125-017-4435-8
    16 sg:pub.10.1007/s00441-013-1634-6
    17 sg:pub.10.1007/s00441-014-2047-x
    18 sg:pub.10.1007/s11892-016-0815-6
    19 sg:pub.10.1023/a:1026032005521
    20 sg:pub.10.1038/35069068
    21 sg:pub.10.1038/eye.2017.27
    22 sg:pub.10.1038/nmeth.2019
    23 sg:pub.10.1038/nrendo.2017.161
    24 sg:pub.10.1038/s41598-016-0028-x
    25 sg:pub.10.1038/s41598-017-09068-6
    26 sg:pub.10.1385/1-59259-811-0:313
    27 schema:datePublished 2019-07-18
    28 schema:datePublishedReg 2019-07-18
    29 schema:description A thinning of the inner retina is one of the earliest potential markers of neuroretinal damage in diabetic subjects. The histological background is uncertain; retinal ganglion cell (RGC) loss and changes in the structure or thickness of the inner plexiform layer (IPL) have been suspected. Studies conducted on animal models on RGC pathology gave contradictory results. Hereby we present RGC numbers, distribution patterns and IPL thickness from Zucker Diabetic Fatty (ZDF) rats. After labelling RGCs on retinal whole mounts, isodensity maps were constructed, RGC numbers and distribution patterns analysed using a custom-built algorithm, enabling point-by-point comparison. There was no change in staining characteristics of the antibodies and no significant difference in average RGC densities was found compared to controls. The distribution patterns were also comparable and no significant difference was found in IPL thickness and stratification or in the number of apoptotic cells in the ganglion cell layer (GCL). Our results provide a detailed evaluation of the inner retina and exclude major RGC loss in ZDF rats and suggest that other factors could serve as a potential explanation for inner retinal thinning in clinical studies. Our custom-built method could be adopted for the assessment of other animal or human retinas.
    30 schema:genre article
    31 schema:inLanguage en
    32 schema:isAccessibleForFree true
    33 schema:isPartOf N3016269f5b814f4c818d9198d54ffaf2
    34 N714a5518a40b4c53921278b48b6a68f2
    35 sg:journal.1045337
    36 schema:keywords Diabetic Fatty
    37 IPL thickness
    38 Possible Ganglion Cell Loss
    39 RGC density
    40 RGC loss
    41 RGC number
    42 RGC pathology
    43 RGCs
    44 ZDF rats
    45 Zucker diabetic fatty
    46 algorithm
    47 animal models
    48 animals
    49 antibodies
    50 apoptotic cells
    51 assessment
    52 average RGC densities
    53 background
    54 cell layer
    55 cell loss
    56 cells
    57 changes
    58 characteristics
    59 clinical studies
    60 comparison
    61 contradictory results
    62 control
    63 custom-built method
    64 damage
    65 density
    66 detailed evaluation
    67 diabetic subjects
    68 differences
    69 distribution patterns
    70 earliest potential markers
    71 evaluation
    72 explanation
    73 factors
    74 fatty
    75 ganglion cell layer
    76 ganglion cell loss
    77 histological background
    78 human retina
    79 inner plexiform layer
    80 inner retina
    81 inner retinal thinning
    82 isodensity maps
    83 layer
    84 loss
    85 major RGC loss
    86 maps
    87 markers
    88 method
    89 model
    90 mount
    91 neuroretinal damage
    92 number
    93 pathology
    94 patterns
    95 plexiform layer
    96 point
    97 point comparison
    98 potential explanation
    99 potential marker
    100 rats
    101 results
    102 retina
    103 retinal ganglion cell loss
    104 retinal thinning
    105 retinal whole mounts
    106 significant differences
    107 stratification
    108 structure
    109 study
    110 subjects
    111 thickness
    112 thinning
    113 whole mounts
    114 schema:name Detailed Evaluation of Possible Ganglion Cell Loss in the Retina of Zucker Diabetic Fatty (ZDF) Rats
    115 schema:pagination 10463
    116 schema:productId N35845fc629ea4490ae072428c0a4442d
    117 N5804da472f36430889eabb66e24e55db
    118 Nd215133d3e194f8a83c6c63d6a24dd48
    119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1118092357
    120 https://doi.org/10.1038/s41598-019-46879-1
    121 schema:sdDatePublished 2022-01-01T18:54
    122 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    123 schema:sdPublisher N7fab28e4190049a49a43f72a7acf8ba4
    124 schema:url https://doi.org/10.1038/s41598-019-46879-1
    125 sgo:license sg:explorer/license/
    126 sgo:sdDataset articles
    127 rdf:type schema:ScholarlyArticle
    128 N07988d9b034947629927c71112059fa1 rdf:first sg:person.010025467347.19
    129 rdf:rest N6f522a6a39e04e2ca622fd457f67c53b
    130 N14df0a0e6c614e9991b86c8722d653c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    131 schema:name Animals
    132 rdf:type schema:DefinedTerm
    133 N2063b0bc71ce44a3b867313ee5390d3e rdf:first sg:person.0631267044.97
    134 rdf:rest N566d388fe50b46fbb91a5366ba1aa803
    135 N242b54e05a3045f5b62c2bed24eff795 rdf:first sg:person.014543626542.79
    136 rdf:rest N834412eeaece4bde98d71760ca358227
    137 N3016269f5b814f4c818d9198d54ffaf2 schema:issueNumber 1
    138 rdf:type schema:PublicationIssue
    139 N35845fc629ea4490ae072428c0a4442d schema:name doi
    140 schema:value 10.1038/s41598-019-46879-1
    141 rdf:type schema:PropertyValue
    142 N405aaecc0cb34a2780e36e75dabf2aa9 rdf:first sg:person.012216010747.36
    143 rdf:rest N2063b0bc71ce44a3b867313ee5390d3e
    144 N410e439cc41342c3a973d14373fa0d2a rdf:first sg:person.013565440475.20
    145 rdf:rest Ncccb91d193c447d79f62df1c4b7106b1
    146 N4b862f1563b9426681c3b35ca8d0c1ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Male
    148 rdf:type schema:DefinedTerm
    149 N566d388fe50b46fbb91a5366ba1aa803 rdf:first sg:person.01346751160.38
    150 rdf:rest N07988d9b034947629927c71112059fa1
    151 N5804da472f36430889eabb66e24e55db schema:name dimensions_id
    152 schema:value pub.1118092357
    153 rdf:type schema:PropertyValue
    154 N5f19da371bb943d9b2962f81ac2f5c32 schema:affiliation grid-institutes:grid.11804.3c
    155 schema:familyName Dávid
    156 schema:givenName Csaba
    157 rdf:type schema:Person
    158 N6bc2623626884d3baa984c25d526196d rdf:first N5f19da371bb943d9b2962f81ac2f5c32
    159 rdf:rest Nf43032216cf7468bb0fe94dde53a5509
    160 N6f522a6a39e04e2ca622fd457f67c53b rdf:first N715bf74a11ec48ec8a07d82ad048628e
    161 rdf:rest N87bb2eaf64594be080e4faec3816c25d
    162 N714a5518a40b4c53921278b48b6a68f2 schema:volumeNumber 9
    163 rdf:type schema:PublicationVolume
    164 N715bf74a11ec48ec8a07d82ad048628e schema:affiliation grid-institutes:grid.11804.3c
    165 schema:familyName Almási
    166 schema:givenName Zsuzsanna
    167 rdf:type schema:Person
    168 N72cd7defa17e4aefad739ea6b4996159 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Body Weight
    170 rdf:type schema:DefinedTerm
    171 N7650b4b9bfa54d8884108dc876734333 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Rats, Zucker
    173 rdf:type schema:DefinedTerm
    174 N7fab28e4190049a49a43f72a7acf8ba4 schema:name Springer Nature - SN SciGraph project
    175 rdf:type schema:Organization
    176 N80989cc342db4d5c958199e3c39c8e8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Diabetes Mellitus, Experimental
    178 rdf:type schema:DefinedTerm
    179 N834412eeaece4bde98d71760ca358227 rdf:first sg:person.01332471212.34
    180 rdf:rest N95d793176c6d422a8555458757d1570f
    181 N83640e5ecc81476ba921afa8cb5aea86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    182 schema:name Rats
    183 rdf:type schema:DefinedTerm
    184 N87bb2eaf64594be080e4faec3816c25d rdf:first sg:person.01010512720.51
    185 rdf:rest N242b54e05a3045f5b62c2bed24eff795
    186 N95d793176c6d422a8555458757d1570f rdf:first sg:person.01063426430.82
    187 rdf:rest N410e439cc41342c3a973d14373fa0d2a
    188 Naa945e79104d46aa95660d63dcd1b955 rdf:first sg:person.0617553001.22
    189 rdf:rest N6bc2623626884d3baa984c25d526196d
    190 Nab965cf116574aad964b8669075f673d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    191 schema:name Optic Nerve
    192 rdf:type schema:DefinedTerm
    193 Nba06edbc02414486b53b5e90778b5e55 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    194 schema:name Retinal Ganglion Cells
    195 rdf:type schema:DefinedTerm
    196 Ncccb91d193c447d79f62df1c4b7106b1 rdf:first sg:person.0576734502.27
    197 rdf:rest Naa945e79104d46aa95660d63dcd1b955
    198 Nd215133d3e194f8a83c6c63d6a24dd48 schema:name pubmed_id
    199 schema:value 31320684
    200 rdf:type schema:PropertyValue
    201 Nd3f4924b7805409cbaa408d7a7c128d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    202 schema:name Apoptosis
    203 rdf:type schema:DefinedTerm
    204 Nf43032216cf7468bb0fe94dde53a5509 rdf:first sg:person.0723452446.75
    205 rdf:rest rdf:nil
    206 Nfed0b7758496464d82783c7a60c444cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    207 schema:name Blood Glucose
    208 rdf:type schema:DefinedTerm
    209 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    210 schema:name Medical and Health Sciences
    211 rdf:type schema:DefinedTerm
    212 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
    213 schema:name Clinical Sciences
    214 rdf:type schema:DefinedTerm
    215 sg:grant.4188749 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-46879-1
    216 rdf:type schema:MonetaryGrant
    217 sg:journal.1045337 schema:issn 2045-2322
    218 schema:name Scientific Reports
    219 schema:publisher Springer Nature
    220 rdf:type schema:Periodical
    221 sg:person.010025467347.19 schema:affiliation grid-institutes:grid.11804.3c
    222 schema:familyName Dékány
    223 schema:givenName Bulcsú
    224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010025467347.19
    225 rdf:type schema:Person
    226 sg:person.01010512720.51 schema:affiliation grid-institutes:grid.11804.3c
    227 schema:familyName Énzsöly
    228 schema:givenName Anna
    229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010512720.51
    230 rdf:type schema:Person
    231 sg:person.01063426430.82 schema:affiliation grid-institutes:grid.11804.3c
    232 schema:familyName Mátyás
    233 schema:givenName Csaba
    234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063426430.82
    235 rdf:type schema:Person
    236 sg:person.012216010747.36 schema:affiliation grid-institutes:grid.11804.3c
    237 schema:familyName Hajdú
    238 schema:givenName Rozina I.
    239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012216010747.36
    240 rdf:type schema:Person
    241 sg:person.01332471212.34 schema:affiliation grid-institutes:grid.11804.3c
    242 schema:familyName Radovits
    243 schema:givenName Tamás
    244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01332471212.34
    245 rdf:type schema:Person
    246 sg:person.01346751160.38 schema:affiliation grid-institutes:grid.11804.3c
    247 schema:familyName Szabó
    248 schema:givenName Klaudia
    249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346751160.38
    250 rdf:type schema:Person
    251 sg:person.013565440475.20 schema:affiliation grid-institutes:grid.11804.3c
    252 schema:familyName Oláh
    253 schema:givenName Attila
    254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013565440475.20
    255 rdf:type schema:Person
    256 sg:person.014543626542.79 schema:affiliation grid-institutes:grid.11804.3c
    257 schema:familyName Szabó
    258 schema:givenName Arnold
    259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014543626542.79
    260 rdf:type schema:Person
    261 sg:person.0576734502.27 schema:affiliation grid-institutes:grid.11804.3c
    262 schema:familyName Szél
    263 schema:givenName Ágoston
    264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576734502.27
    265 rdf:type schema:Person
    266 sg:person.0617553001.22 schema:affiliation grid-institutes:None
    267 schema:familyName Somfai
    268 schema:givenName Gábor M.
    269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617553001.22
    270 rdf:type schema:Person
    271 sg:person.0631267044.97 schema:affiliation grid-institutes:grid.11804.3c
    272 schema:familyName Laurik
    273 schema:givenName Lenke K.
    274 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631267044.97
    275 rdf:type schema:Person
    276 sg:person.0723452446.75 schema:affiliation grid-institutes:grid.11804.3c
    277 schema:familyName Lukáts
    278 schema:givenName Ákos
    279 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0723452446.75
    280 rdf:type schema:Person
    281 sg:pub.10.1007/s00125-003-1177-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051885969
    282 https://doi.org/10.1007/s00125-003-1177-6
    283 rdf:type schema:CreativeWork
    284 sg:pub.10.1007/s00125-017-4435-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091909657
    285 https://doi.org/10.1007/s00125-017-4435-8
    286 rdf:type schema:CreativeWork
    287 sg:pub.10.1007/s00441-013-1634-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041167220
    288 https://doi.org/10.1007/s00441-013-1634-6
    289 rdf:type schema:CreativeWork
    290 sg:pub.10.1007/s00441-014-2047-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034140930
    291 https://doi.org/10.1007/s00441-014-2047-x
    292 rdf:type schema:CreativeWork
    293 sg:pub.10.1007/s11892-016-0815-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026116413
    294 https://doi.org/10.1007/s11892-016-0815-6
    295 rdf:type schema:CreativeWork
    296 sg:pub.10.1023/a:1026032005521 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012967748
    297 https://doi.org/10.1023/a:1026032005521
    298 rdf:type schema:CreativeWork
    299 sg:pub.10.1038/35069068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004850591
    300 https://doi.org/10.1038/35069068
    301 rdf:type schema:CreativeWork
    302 sg:pub.10.1038/eye.2017.27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084128034
    303 https://doi.org/10.1038/eye.2017.27
    304 rdf:type schema:CreativeWork
    305 sg:pub.10.1038/nmeth.2019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026706357
    306 https://doi.org/10.1038/nmeth.2019
    307 rdf:type schema:CreativeWork
    308 sg:pub.10.1038/nrendo.2017.161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100474408
    309 https://doi.org/10.1038/nrendo.2017.161
    310 rdf:type schema:CreativeWork
    311 sg:pub.10.1038/s41598-016-0028-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079403223
    312 https://doi.org/10.1038/s41598-016-0028-x
    313 rdf:type schema:CreativeWork
    314 sg:pub.10.1038/s41598-017-09068-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091255902
    315 https://doi.org/10.1038/s41598-017-09068-6
    316 rdf:type schema:CreativeWork
    317 sg:pub.10.1385/1-59259-811-0:313 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018267824
    318 https://doi.org/10.1385/1-59259-811-0:313
    319 rdf:type schema:CreativeWork
    320 grid-institutes:None schema:alternateName Retinology Unit, Pallas Kliniken, Olten, Switzerland
    321 schema:name Department of Ophthalmology, Semmelweis University, Budapest, Hungary
    322 Retinology Unit, Pallas Kliniken, Olten, Switzerland
    323 rdf:type schema:Organization
    324 grid-institutes:grid.11804.3c schema:alternateName Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary
    325 Department of Ophthalmology, Semmelweis University, Budapest, Hungary
    326 Heart and Vascular Center, Semmelweis University, Budapest, Hungary
    327 schema:name Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary
    328 Department of Ophthalmology, Semmelweis University, Budapest, Hungary
    329 Heart and Vascular Center, Semmelweis University, Budapest, Hungary
    330 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...