Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink bollworm from China View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-05-16

AUTHORS

Ling Wang, Jintao Wang, Yuemin Ma, Peng Wan, Kaiyu Liu, Shengbo Cong, Yutao Xiao, Dong Xu, Kongming Wu, Jeffrey A. Fabrick, Xianchun Li, Bruce E. Tabashnik

ABSTRACT

Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) are cultivated extensively, but rapid evolution of resistance by pests reduces their efficacy. We report a 3,370-bp insertion in a cadherin gene associated with resistance to Bt toxin Cry1Ac in the pink bollworm (Pectinophora gossypiella), a devastating global cotton pest. We found the allele (r15) harboring this insertion in a field population from China. The insertion is a miniature inverted repeat transposable element (MITE) that contains two additional transposons and produces two mis-spliced transcript variants (r15A and r15B). A strain homozygous for r15 had 290-fold resistance to Cry1Ac, little or no cross-resistance to Cry2Ab, and completed its life cycle on Bt cotton producing Cry1Ac. Inheritance of resistance was recessive and tightly linked with r15. For transformed insect cells, susceptibility to Cry1Ac was greater for cells producing the wild-type cadherin than for cells producing the r15 mutant proteins. Recombinant cadherin protein occurred on the cell surface in cells transformed with the wild-type or r15A sequences, but not in cells transformed with the r15B sequence. The similar resistance of pink bollworm to Cry1Ac in laboratory- and field-selected insects from China, India and the U.S. provides a basis for developing international resistance management practices. More... »

PAGES

7479

Journal

TITLE

Scientific Reports

ISSUE

1

VOLUME

9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-43889-x

DOI

http://dx.doi.org/10.1038/s41598-019-43889-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1114459942

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31097777


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31 schema:description Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) are cultivated extensively, but rapid evolution of resistance by pests reduces their efficacy. We report a 3,370-bp insertion in a cadherin gene associated with resistance to Bt toxin Cry1Ac in the pink bollworm (Pectinophora gossypiella), a devastating global cotton pest. We found the allele (r15) harboring this insertion in a field population from China. The insertion is a miniature inverted repeat transposable element (MITE) that contains two additional transposons and produces two mis-spliced transcript variants (r15A and r15B). A strain homozygous for r15 had 290-fold resistance to Cry1Ac, little or no cross-resistance to Cry2Ab, and completed its life cycle on Bt cotton producing Cry1Ac. Inheritance of resistance was recessive and tightly linked with r15. For transformed insect cells, susceptibility to Cry1Ac was greater for cells producing the wild-type cadherin than for cells producing the r15 mutant proteins. Recombinant cadherin protein occurred on the cell surface in cells transformed with the wild-type or r15A sequences, but not in cells transformed with the r15B sequence. The similar resistance of pink bollworm to Cry1Ac in laboratory- and field-selected insects from China, India and the U.S. provides a basis for developing international resistance management practices.
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39 Bt toxin Cry1Ac
40 China
41 Cry1Ac
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43 India
44 R15
45 U.S.
46 additional transposons
47 alleles
48 basis
49 bollworm
50 bp insertion
51 cadherin
52 cadherin gene
53 cadherin protein
54 cell surface
55 cells
56 cotton
57 cotton pests
58 crops
59 cycle
60 efficacy
61 elements
62 evolution
63 field populations
64 genes
65 inheritance
66 inheritance of resistance
67 insect cells
68 insecticidal proteins
69 insects
70 insertion
71 laboratory
72 life cycle
73 management practices
74 miniature inverted repeat transposable elements
75 mis-splicing
76 mutant proteins
77 pests
78 pink bollworm
79 population
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81 protein
82 rapid evolution
83 repeat transposable elements
84 resistance
85 resistance management practices
86 sequence
87 similar resistance
88 strains
89 surface
90 susceptibility
91 thuringiensis
92 toxin Cry1Ac
93 transcript variants
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