Incomplete cell disruption of resistant microbes. View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Robert Starke, Nico Jehmlich, Trinidad Alfaro, Alice Dohnalkova, Petr Capek, Sheryl L Bell, Kirsten S Hofmockel

ABSTRACT

Biomolecules for OMIC analysis of microbial communities are commonly extracted by bead-beating or ultra-sonication, but both showed varying yields. In addition to that, different disruption pressures are necessary to lyse bacteria and fungi. However, the disruption efficiency and yields comparing bead-beating and ultra-sonication of different biological material have not yet been demonstrated. Here, we show that ultra-sonication in a bath transfers three times more energy than bead-beating over 10 min. TEM imaging revealed intact gram-positive bacterial and fungal cells whereas the gram-negative bacterial cells were destroyed beyond recognition after 10 min of ultra-sonication. DNA extraction using 10 min of bead-beating revealed higher yields for fungi but the extraction efficiency was at least three-fold lower considering its larger genome. By our critical viewpoint, we encourage the review of the commonly used extraction techniques as we provide evidence for a potential underrepresentation of resistant microbes, particularly fungi, in ecological studies. More... »

PAGES

5618

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-42188-9

DOI

http://dx.doi.org/10.1038/s41598-019-42188-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113185016

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30948770


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