Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance. View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Bruna H Marcon, Patrícia Shigunov, Lucia Spangenberg, Isabela Tiemy Pereira, Alessandra Melo de Aguiar, Rocío Amorín, Carmen K Rebelatto, Alejandro Correa, Bruno Dallagiovanna

ABSTRACT

The adipogenic process is characterized by the expression of adipocyte differentiation markers that lead to changes in cell metabolism and to the accumulation of lipid droplets. Moreover, during early adipogenesis, cells undergo a strong downregulation of translational activity with a decrease in cell size, proliferation and migration. In the present study, we identified that after 24 hours of adipogenic induction, human adipose tissue-derived stem cells (hASCs) undergo a G1-cell cycle arrest consistent with reduced proliferation, and this effect was correlated with a shift in polysome profile with an enrichment of the monosomal fraction and a reduction of the polysomal fraction. Polysome profiling analysis also revealed that this change in the monosomal/polysomal ratio was related to a strong downregulation of cell cycle and proliferation genes, such as cyclins and cyclin-dependent kinases (CDKs). Comparing total and polysome-associated mRNA sequencing, we also observed that this downregulation was mostly due to a reduction of cell cycle and proliferation transcripts via control of total mRNA abundance, rather than by translational control. More... »

PAGES

5611

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-42005-3

DOI

http://dx.doi.org/10.1038/s41598-019-42005-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1113184996

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30948750


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