Ontology type: schema:ScholarlyArticle Open Access: True
2019-12
AUTHORSSara A. Zahran, Marwa Ali-Tammam, Abdelgawad M. Hashem, Ramy K. Aziz, Amal E. Ali
ABSTRACTThe gut microbiota enriches the human gene pool and contributes to xenobiotic metabolism. Microbial azoreductases modulate the reduction of azo-bonds, activating produgs and azo polymer-coated dosage forms, or degrading food additives. Here, we aimed to screen the healthy human gut microbiota for food colorant-reducing activity and to characterize factors modulating it. Four representative isolates from screened fecal samples were identified as E. coli (AZO-Ec), E. faecalis (AZO-Ef), E. avium (AZO-Ev) and B. cereus (AZO-Bc). Both AZO-Ef and AZO-Ev decolorized amaranth aerobically and microaerophilically while AZO-Ec and AZO-Bc had higher aerobic reduction rates. The isolates varied in their activities against different dyes, and the azo-reduction activity mostly followed zero-order reaction kinetics, with a few exceptions. Additionally, the isolates had different pH dependence, e.g., AZO-Ec was not affected by pH variation while AZO-Bc exhibited variable degradation kinetics at different pH levels. Cell-free extracts showed NADH-dependent enzymatic activities 14-19 times higher than extracellular fractions. FMN did not affect the reducing activity of AZO-Ef cell-free extract, whereas AZO-Ec, AZO-Ev and AZO-Bc had significantly higher reduction rates in its presence (P values = 0.02, 0.0001 and 0.02, respectively). Using Degenerate primers allowed the amplification of azoreductase genes, whose sequences were 98-99% similar to genes encoding FMN-dependent-NADH azoreductases. More... »
PAGES5508
http://scigraph.springernature.com/pub.10.1038/s41598-019-41894-8
DOIhttp://dx.doi.org/10.1038/s41598-019-41894-8
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1113172350
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30940826
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Microbiology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Future University in Egypt",
"id": "https://www.grid.ac/institutes/grid.440865.b",
"name": [
"Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, 12311, New Cairo, Egypt"
],
"type": "Organization"
},
"familyName": "Zahran",
"givenName": "Sara A.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Future University in Egypt",
"id": "https://www.grid.ac/institutes/grid.440865.b",
"name": [
"Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, 12311, New Cairo, Egypt"
],
"type": "Organization"
},
"familyName": "Ali-Tammam",
"givenName": "Marwa",
"type": "Person"
},
{
"affiliation": {
"alternateName": "British University in Egypt",
"id": "https://www.grid.ac/institutes/grid.440862.c",
"name": [
"Department of Microbiology and Immunology, Faculty of Pharmacy, The British University in Egypt, Shorouk City, Egypt"
],
"type": "Organization"
},
"familyName": "Hashem",
"givenName": "Abdelgawad M.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Cairo University",
"id": "https://www.grid.ac/institutes/grid.7776.1",
"name": [
"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt"
],
"type": "Organization"
},
"familyName": "Aziz",
"givenName": "Ramy K.",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Cairo University",
"id": "https://www.grid.ac/institutes/grid.7776.1",
"name": [
"Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, 12311, New Cairo, Egypt",
"Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt"
],
"type": "Organization"
},
"familyName": "Ali",
"givenName": "Amal E.",
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.4172/2155-6199.1000183",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000298175"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/npjbiofilms.2016.3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000475133",
"https://doi.org/10.1038/npjbiofilms.2016.3"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ijpharm.2008.07.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002311951"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.anaerobe.2011.12.006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002673741"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1550-7408.1999.tb04612.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002950465"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3402/mehd.v26.26050",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004118224"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature11552",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004952443",
"https://doi.org/10.1038/nature11552"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bjm.2015.11.013",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007135662"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1471-2091-11-13",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007202118",
"https://doi.org/10.1186/1471-2091-11-13"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m710070200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007412399"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4172/2155-6199.1000199",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008464798"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-2836(05)80360-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013618994"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.phrs.2012.07.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013896613"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1080/10717540802518157",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014799289"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1574-6976.2008.00107.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014835876"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2903/j.efsa.2009.1331",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017062400"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1432-1033.1970.tb00919.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018623733"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.procbio.2011.12.013",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018907590"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-61779-501-5_39",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019042951",
"https://doi.org/10.1007/978-1-61779-501-5_39"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1136/gut.29.8.1035",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019597180"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0165-6147(84)90532-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019830356"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0165-6147(84)90532-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019830356"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.17179/excli2014-642",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020163840"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.1307797110",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022041461"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2903/j.efsa.2010.1540",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022952675"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-015-6798-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023396262",
"https://doi.org/10.1007/s00253-015-6798-4"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.pep.2003.12.016",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026909006"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s40201-014-0151-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028253287",
"https://doi.org/10.1186/s40201-014-0151-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s40201-014-0151-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028253287",
"https://doi.org/10.1186/s40201-014-0151-1"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m008083200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029722528"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.68.8.3948-3955.2002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034061998"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/jam.12294",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035044619"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1017/s0007114512005235",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038473146"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1017/s0007114512005235",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038473146"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m513345200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041284313"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.66.4.1429-1434.2000",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041800734"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4103/0971-6580.139797",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042051046"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkv1189",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042394201"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.jim.2900734",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043475182",
"https://doi.org/10.1038/sj.jim.2900734"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1757-4749-4-16",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044990740",
"https://doi.org/10.1186/1757-4749-4-16"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0609073103",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045281309"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2672.2010.04675.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047181280"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2672.2010.04675.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047181280"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-006-0836-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048273076",
"https://doi.org/10.1007/s00253-006-0836-1"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-006-0836-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048273076",
"https://doi.org/10.1007/s00253-006-0836-1"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jcm.39.11.4052-4057.2001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049458408"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2903/j.efsa.2014.3765",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049995705"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0006-2952(70)90338-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050169547"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0006-2952(70)90338-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050169547"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/aem.02289-10",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051272574"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.abb.2007.03.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051344978"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3109/10408419209114557",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053036747"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/acs.est.5b02003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055087812"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1080/08910600802169630",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1058345963"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/omi.2014.0018",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059304065"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/jmm.0.05062-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060391890"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/mic.0.2008/019877-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060396469"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1099/mic.0.27805-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060397382"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.18773/austprescr.1994.046",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1068688635"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/187569210792246326",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069238349"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/187569212802510030",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069238538"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.21775/cimb.011.059",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069253017"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.21775/cimb.012.043",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069253034"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1077202556",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078182523",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078466598",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082656509",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jfda.2017.01.005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083820890"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.eng.2017.01.008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084041555"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bcj20160510",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1085469420"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bcj20160510",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1085469420"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.aag2770",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1086134447"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.btre.2017.06.007",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090358537"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s40643-018-0206-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1103666772",
"https://doi.org/10.1186/s40643-018-0206-8"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/s40643-018-0206-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1103666772",
"https://doi.org/10.1186/s40643-018-0206-8"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1080/17425255.2018.1530216",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1107330518"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-12",
"datePublishedReg": "2019-12-01",
"description": "The gut microbiota enriches the human gene pool and contributes to xenobiotic metabolism. Microbial azoreductases modulate the reduction of azo-bonds, activating produgs and azo polymer-coated dosage forms, or degrading food additives. Here, we aimed to screen the healthy human gut microbiota for food colorant-reducing activity and to characterize factors modulating it. Four representative isolates from screened fecal samples were identified as E. coli (AZO-Ec), E. faecalis (AZO-Ef), E. avium (AZO-Ev) and B. cereus (AZO-Bc). Both AZO-Ef and AZO-Ev decolorized amaranth aerobically and microaerophilically while AZO-Ec and AZO-Bc had higher aerobic reduction rates. The isolates varied in their activities against different dyes, and the azo-reduction activity mostly followed zero-order reaction kinetics, with a few exceptions. Additionally, the isolates had different pH dependence, e.g., AZO-Ec was not affected by pH variation while AZO-Bc exhibited variable degradation kinetics at different pH levels. Cell-free extracts showed NADH-dependent enzymatic activities 14-19 times higher than extracellular fractions. FMN did not affect the reducing activity of AZO-Ef cell-free extract, whereas AZO-Ec, AZO-Ev and AZO-Bc had significantly higher reduction rates in its presence (P\u2009values\u2009=\u20090.02, 0.0001 and 0.02, respectively). Using Degenerate primers allowed the amplification of azoreductase genes, whose sequences were 98-99% similar to genes encoding FMN-dependent-NADH azoreductases.",
"genre": "research_article",
"id": "sg:pub.10.1038/s41598-019-41894-8",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1045337",
"issn": [
"2045-2322"
],
"name": "Scientific Reports",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "9"
}
],
"name": "Azoreductase activity of dye-decolorizing bacteria isolated from the human gut microbiota",
"pagination": "5508",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41598-019-41894-8"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"4abaea51a3c1b0c387763306722e6fa3b9eac57755c77f5e084f68f03af93235"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1113172350"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101563288"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30940826"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41598-019-41894-8",
"https://app.dimensions.ai/details/publication/pub.1113172350"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T09:10",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56155_00000006.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/s41598-019-41894-8"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41894-8'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41894-8'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41894-8'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41894-8'
This table displays all metadata directly associated to this object as RDF triples.
308 TRIPLES
21 PREDICATES
97 URIs
21 LITERALS
9 BLANK NODES