PMCA-replicated PrPD in urine of vCJD patients maintains infectivity and strain characteristics of brain PrPD: Transmission study View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Ignazio Cali, Jody Lavrich, Fabio Moda, Diane Kofskey, Satish Kumar Nemani, Brian Appleby, Fabrizio Tagliavini, Claudio Soto, Pierluigi Gambetti, Silvio Notari

ABSTRACT

The presence of abnormal, disease-related prion protein (PrPD) has recently been demonstrated by protein misfolding cyclic amplification (PMCA) in urine of patients affected with variant Creutzfeldt-Jakob disease (vCJD), a prion disease typically acquired from consumption of prion contaminated bovine meat. The complexity and multistage process of urine excretion along with the obligatory use of PMCA raise the issue of whether strain characteristics of the PrPD present in vCJD brains, such as infectivity and phenotype determination, are maintained in urine excreted PrPD and following amplification by PMCA. We inoculated transgenic mice expressing normal human PrP with amplified urine and brain homogenate achieving the same 100% attack rate, similar incubation periods (in both cases extremely long) and histopathological features as for type and severity of the lesions. Furthermore, PrPD characteristics analyzed by immunoblot and conformational stability immunoassay were indistinguishable. Inoculation of raw vCJD urine caused no disease, confirming the extremely low concentration of PrPD in vCJD urine. These findings show that strain characteristics of vCJD brain PrPD, including infectivity, are preserved in PrPD present in urine and are faithfully amplified by means of PMCA; moreover, they suggest that the PrPD urine test might allow for the diagnosis and identification of disease subtype also in sporadic CJD. More... »

PAGES

5191

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-41694-0

DOI

http://dx.doi.org/10.1038/s41598-019-41694-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112985347

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30914754


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Case Western Reserve University, Department of Pathology, Cleveland, Ohio, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cali", 
        "givenName": "Ignazio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Case Western Reserve University, Department of Pathology, Cleveland, Ohio, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lavrich", 
        "givenName": "Jody", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Neurologico Carlo Besta", 
          "id": "https://www.grid.ac/institutes/grid.417894.7", 
          "name": [
            "Fondazione IRCCS Istituto Neurologico Carlo Besta, Neurology 5 and Neuropathology Unit, Milano, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moda", 
        "givenName": "Fabio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Case Western Reserve University, Department of Pathology, Cleveland, Ohio, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kofskey", 
        "givenName": "Diane", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Case Western Reserve University, Department of Pathology, Cleveland, Ohio, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nemani", 
        "givenName": "Satish Kumar", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Case Western Reserve University, National Prion Disease Pathology Surveillance Center and Departments of Neurology and Psychiatry, Cleveland, Ohio, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Appleby", 
        "givenName": "Brian", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Istituto Neurologico Carlo Besta", 
          "id": "https://www.grid.ac/institutes/grid.417894.7", 
          "name": [
            "Fondazione IRCCS Istituto Neurologico Carlo Besta, Neurology 5 and Neuropathology Unit, Milano, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tagliavini", 
        "givenName": "Fabrizio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas Health Science Center at Houston", 
          "id": "https://www.grid.ac/institutes/grid.267308.8", 
          "name": [
            "University of Texas Health Science Center, Department of Neurology, Houston, Texas, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Soto", 
        "givenName": "Claudio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Case Western Reserve University, Department of Pathology, Cleveland, Ohio, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gambetti", 
        "givenName": "Pierluigi", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Case Western Reserve University, Department of Pathology, Cleveland, Ohio, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Notari", 
        "givenName": "Silvio", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.cell.2005.02.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000151064"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/brain/awp196", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001056335"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.32.19173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001830564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m610051200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004744496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri3553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009880767", 
          "https://doi.org/10.1038/nri3553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)63105-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013215860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/38925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013318654", 
          "https://doi.org/10.1038/38925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/38925", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013318654", 
          "https://doi.org/10.1038/38925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0307825100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014959706"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0002-9440(10)61254-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017146762"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1801.110589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019361504"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.161794", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020003733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.01544-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022365860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1001/jamaneurol.2016.3733", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023830539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1109.050371", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027331931"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2008.08.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031968140"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.26.15457", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034207954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00401-010-0761-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034715115", 
          "https://doi.org/10.1007/s00401-010-0761-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00401-010-0761-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034715115", 
          "https://doi.org/10.1007/s00401-010-0761-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/39057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037079750", 
          "https://doi.org/10.1038/39057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/39057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037079750", 
          "https://doi.org/10.1038/39057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.20.9678", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037360856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.2467-05.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038193081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2012.140548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040778276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1502.081173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045856525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0008765", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049300911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.22.10940", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050592984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1404401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053072878"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1118829", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062452678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078847176", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2306.161734", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084953056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/bs.pmbts.2017.06.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1090954662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4939-7244-9_18", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091408903", 
          "https://doi.org/10.1007/978-1-4939-7244-9_18"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/pathogens6040060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1093007208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s40478-017-0503-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100258856", 
          "https://doi.org/10.1186/s40478-017-0503-z"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "The presence of abnormal, disease-related prion protein (PrPD) has recently been demonstrated by protein misfolding cyclic amplification (PMCA) in urine of patients affected with variant Creutzfeldt-Jakob disease (vCJD), a prion disease typically acquired from consumption of prion contaminated bovine meat. The complexity and multistage process of urine excretion along with the obligatory use of PMCA raise the issue of whether strain characteristics of the PrPD present in vCJD brains, such as infectivity and phenotype determination, are maintained in urine excreted PrPD and following amplification by PMCA. We inoculated transgenic mice expressing normal human PrP with amplified urine and brain homogenate achieving the same 100% attack rate, similar incubation periods (in both cases extremely long) and histopathological features as for type and severity of the lesions. Furthermore, PrPD characteristics analyzed by immunoblot and conformational stability immunoassay were indistinguishable. Inoculation of raw vCJD urine caused no disease, confirming the extremely low concentration of PrPD in vCJD urine. These findings show that strain characteristics of vCJD brain PrPD, including infectivity, are preserved in PrPD present in urine and are faithfully amplified by means of PMCA; moreover, they suggest that the PrPD urine test might allow for the diagnosis and identification of disease subtype also in sporadic CJD.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-019-41694-0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.3624337", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3803321", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "PMCA-replicated PrPD in urine of vCJD patients maintains infectivity and strain characteristics of brain PrPD: Transmission study", 
    "pagination": "5191", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "581be0ad9b8d1023f44bda5d0e158b47c5ed82c55e0469ecc3524678e63fb520"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30914754"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-019-41694-0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112985347"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-019-41694-0", 
      "https://app.dimensions.ai/details/publication/pub.1112985347"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:48", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130792_00000006.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-019-41694-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41694-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41694-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41694-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41694-0'


 

This table displays all metadata directly associated to this object as RDF triples.

233 TRIPLES      21 PREDICATES      61 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-019-41694-0 schema:about anzsrc-for:11
2 anzsrc-for:1109
3 schema:author Nd4e61c10e0c4452d8f045323a8f337c8
4 schema:citation sg:pub.10.1007/978-1-4939-7244-9_18
5 sg:pub.10.1007/s00401-010-0761-3
6 sg:pub.10.1038/38925
7 sg:pub.10.1038/39057
8 sg:pub.10.1038/nri3553
9 sg:pub.10.1186/s40478-017-0503-z
10 https://app.dimensions.ai/details/publication/pub.1078847176
11 https://doi.org/10.1001/jamaneurol.2016.3733
12 https://doi.org/10.1016/bs.pmbts.2017.06.011
13 https://doi.org/10.1016/j.cell.2005.02.011
14 https://doi.org/10.1016/j.febslet.2008.08.003
15 https://doi.org/10.1016/s0002-9440(10)61254-0
16 https://doi.org/10.1016/s0002-9440(10)63105-7
17 https://doi.org/10.1056/nejmoa1404401
18 https://doi.org/10.1073/pnas.0307825100
19 https://doi.org/10.1073/pnas.89.22.10940
20 https://doi.org/10.1073/pnas.90.20.9678
21 https://doi.org/10.1073/pnas.93.26.15457
22 https://doi.org/10.1074/jbc.270.32.19173
23 https://doi.org/10.1074/jbc.m110.161794
24 https://doi.org/10.1074/jbc.m610051200
25 https://doi.org/10.1093/brain/awp196
26 https://doi.org/10.1126/science.1118829
27 https://doi.org/10.1128/jvi.01544-15
28 https://doi.org/10.1371/journal.pone.0008765
29 https://doi.org/10.1523/jneurosci.2467-05.2005
30 https://doi.org/10.3201/eid1109.050371
31 https://doi.org/10.3201/eid1502.081173
32 https://doi.org/10.3201/eid1801.110589
33 https://doi.org/10.3201/eid2012.140548
34 https://doi.org/10.3201/eid2306.161734
35 https://doi.org/10.3390/pathogens6040060
36 schema:datePublished 2019-12
37 schema:datePublishedReg 2019-12-01
38 schema:description The presence of abnormal, disease-related prion protein (PrP<sup>D</sup>) has recently been demonstrated by protein misfolding cyclic amplification (PMCA) in urine of patients affected with variant Creutzfeldt-Jakob disease (vCJD), a prion disease typically acquired from consumption of prion contaminated bovine meat. The complexity and multistage process of urine excretion along with the obligatory use of PMCA raise the issue of whether strain characteristics of the PrP<sup>D</sup> present in vCJD brains, such as infectivity and phenotype determination, are maintained in urine excreted PrP<sup>D</sup> and following amplification by PMCA. We inoculated transgenic mice expressing normal human PrP with amplified urine and brain homogenate achieving the same 100% attack rate, similar incubation periods (in both cases extremely long) and histopathological features as for type and severity of the lesions. Furthermore, PrP<sup>D</sup> characteristics analyzed by immunoblot and conformational stability immunoassay were indistinguishable. Inoculation of raw vCJD urine caused no disease, confirming the extremely low concentration of PrP<sup>D</sup> in vCJD urine. These findings show that strain characteristics of vCJD brain PrP<sup>D</sup>, including infectivity, are preserved in PrP<sup>D</sup> present in urine and are faithfully amplified by means of PMCA; moreover, they suggest that the PrP<sup>D</sup> urine test might allow for the diagnosis and identification of disease subtype also in sporadic CJD.
39 schema:genre research_article
40 schema:inLanguage en
41 schema:isAccessibleForFree true
42 schema:isPartOf N254c02d51def49cd9f88e7b8d1f147b1
43 N7feeddb41e344715bd99b57ffbbf8c09
44 sg:journal.1045337
45 schema:name PMCA-replicated PrPD in urine of vCJD patients maintains infectivity and strain characteristics of brain PrPD: Transmission study
46 schema:pagination 5191
47 schema:productId N28536a6d428141bea64ae18807c9cd7e
48 N8ce22314d39d4b92a3f516fdf0538d0a
49 N9e73349d6c5a4432be4edd6e5ad8b249
50 Nb3f0a2ace1304452a6886683e4007f0d
51 Ncf835098a0bb42efa333a9be4d9e3ea1
52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112985347
53 https://doi.org/10.1038/s41598-019-41694-0
54 schema:sdDatePublished 2019-04-11T13:48
55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
56 schema:sdPublisher N8a246f22dc414769aa241867e5947c70
57 schema:url https://www.nature.com/articles/s41598-019-41694-0
58 sgo:license sg:explorer/license/
59 sgo:sdDataset articles
60 rdf:type schema:ScholarlyArticle
61 N06d9b91a36a54b45ad1758c5bfc962ff rdf:first N967a950fd54e4fcead89103ec977140b
62 rdf:rest Nbc257bbe878443139e0c123a6aeb39ae
63 N09114ef5616f49019cf43d31c9d7f6ac schema:affiliation https://www.grid.ac/institutes/grid.267308.8
64 schema:familyName Soto
65 schema:givenName Claudio
66 rdf:type schema:Person
67 N0e136e7733d048f0a68aa4fbf923ca08 schema:affiliation https://www.grid.ac/institutes/grid.67105.35
68 schema:familyName Appleby
69 schema:givenName Brian
70 rdf:type schema:Person
71 N254c02d51def49cd9f88e7b8d1f147b1 schema:volumeNumber 9
72 rdf:type schema:PublicationVolume
73 N27abd15a9269453b9edd5c39112f7f5d schema:affiliation https://www.grid.ac/institutes/grid.67105.35
74 schema:familyName Notari
75 schema:givenName Silvio
76 rdf:type schema:Person
77 N27c275cd194242e693ebad9998bd8ca1 rdf:first N9054f38d93014be5b101a4acb3e7241c
78 rdf:rest Na21cc3a7f4c44d208b328487004eb82f
79 N28536a6d428141bea64ae18807c9cd7e schema:name readcube_id
80 schema:value 581be0ad9b8d1023f44bda5d0e158b47c5ed82c55e0469ecc3524678e63fb520
81 rdf:type schema:PropertyValue
82 N35cd34fd3f8a4dc9be857c788c13d170 rdf:first Na3966bef1c714f66a793d5f1996a5172
83 rdf:rest Nd5920ca70025463a8dd187afea6f33fc
84 N7c80ee3292034325a611ebaffd30ce24 rdf:first Na0f8bd65ee4c4f85af2f01a07b918c56
85 rdf:rest N7f2d959c0e3344cbb1d2c958e8c612eb
86 N7f2d959c0e3344cbb1d2c958e8c612eb rdf:first N09114ef5616f49019cf43d31c9d7f6ac
87 rdf:rest N27c275cd194242e693ebad9998bd8ca1
88 N7feeddb41e344715bd99b57ffbbf8c09 schema:issueNumber 1
89 rdf:type schema:PublicationIssue
90 N83b9f00f50c045faa42959aa5eeee090 rdf:first N0e136e7733d048f0a68aa4fbf923ca08
91 rdf:rest N7c80ee3292034325a611ebaffd30ce24
92 N8a246f22dc414769aa241867e5947c70 schema:name Springer Nature - SN SciGraph project
93 rdf:type schema:Organization
94 N8ce22314d39d4b92a3f516fdf0538d0a schema:name pubmed_id
95 schema:value 30914754
96 rdf:type schema:PropertyValue
97 N9054f38d93014be5b101a4acb3e7241c schema:affiliation https://www.grid.ac/institutes/grid.67105.35
98 schema:familyName Gambetti
99 schema:givenName Pierluigi
100 rdf:type schema:Person
101 N907db415487a4a4d861e2cf43cad93b7 schema:affiliation https://www.grid.ac/institutes/grid.67105.35
102 schema:familyName Cali
103 schema:givenName Ignazio
104 rdf:type schema:Person
105 N967a950fd54e4fcead89103ec977140b schema:affiliation https://www.grid.ac/institutes/grid.67105.35
106 schema:familyName Kofskey
107 schema:givenName Diane
108 rdf:type schema:Person
109 N9e73349d6c5a4432be4edd6e5ad8b249 schema:name doi
110 schema:value 10.1038/s41598-019-41694-0
111 rdf:type schema:PropertyValue
112 Na0a4ff9b803149e4b8a7a7fab8bf38b3 schema:affiliation https://www.grid.ac/institutes/grid.67105.35
113 schema:familyName Nemani
114 schema:givenName Satish Kumar
115 rdf:type schema:Person
116 Na0f8bd65ee4c4f85af2f01a07b918c56 schema:affiliation https://www.grid.ac/institutes/grid.417894.7
117 schema:familyName Tagliavini
118 schema:givenName Fabrizio
119 rdf:type schema:Person
120 Na21cc3a7f4c44d208b328487004eb82f rdf:first N27abd15a9269453b9edd5c39112f7f5d
121 rdf:rest rdf:nil
122 Na3966bef1c714f66a793d5f1996a5172 schema:affiliation https://www.grid.ac/institutes/grid.67105.35
123 schema:familyName Lavrich
124 schema:givenName Jody
125 rdf:type schema:Person
126 Nb3f0a2ace1304452a6886683e4007f0d schema:name nlm_unique_id
127 schema:value 101563288
128 rdf:type schema:PropertyValue
129 Nbc257bbe878443139e0c123a6aeb39ae rdf:first Na0a4ff9b803149e4b8a7a7fab8bf38b3
130 rdf:rest N83b9f00f50c045faa42959aa5eeee090
131 Nccb0c4c6db4f4306b01ad73eabe26292 schema:affiliation https://www.grid.ac/institutes/grid.417894.7
132 schema:familyName Moda
133 schema:givenName Fabio
134 rdf:type schema:Person
135 Ncf835098a0bb42efa333a9be4d9e3ea1 schema:name dimensions_id
136 schema:value pub.1112985347
137 rdf:type schema:PropertyValue
138 Nd4e61c10e0c4452d8f045323a8f337c8 rdf:first N907db415487a4a4d861e2cf43cad93b7
139 rdf:rest N35cd34fd3f8a4dc9be857c788c13d170
140 Nd5920ca70025463a8dd187afea6f33fc rdf:first Nccb0c4c6db4f4306b01ad73eabe26292
141 rdf:rest N06d9b91a36a54b45ad1758c5bfc962ff
142 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
143 schema:name Medical and Health Sciences
144 rdf:type schema:DefinedTerm
145 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
146 schema:name Neurosciences
147 rdf:type schema:DefinedTerm
148 sg:grant.3624337 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41694-0
149 rdf:type schema:MonetaryGrant
150 sg:grant.3803321 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41694-0
151 rdf:type schema:MonetaryGrant
152 sg:journal.1045337 schema:issn 2045-2322
153 schema:name Scientific Reports
154 rdf:type schema:Periodical
155 sg:pub.10.1007/978-1-4939-7244-9_18 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091408903
156 https://doi.org/10.1007/978-1-4939-7244-9_18
157 rdf:type schema:CreativeWork
158 sg:pub.10.1007/s00401-010-0761-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034715115
159 https://doi.org/10.1007/s00401-010-0761-3
160 rdf:type schema:CreativeWork
161 sg:pub.10.1038/38925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013318654
162 https://doi.org/10.1038/38925
163 rdf:type schema:CreativeWork
164 sg:pub.10.1038/39057 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037079750
165 https://doi.org/10.1038/39057
166 rdf:type schema:CreativeWork
167 sg:pub.10.1038/nri3553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009880767
168 https://doi.org/10.1038/nri3553
169 rdf:type schema:CreativeWork
170 sg:pub.10.1186/s40478-017-0503-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1100258856
171 https://doi.org/10.1186/s40478-017-0503-z
172 rdf:type schema:CreativeWork
173 https://app.dimensions.ai/details/publication/pub.1078847176 schema:CreativeWork
174 https://doi.org/10.1001/jamaneurol.2016.3733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023830539
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/bs.pmbts.2017.06.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090954662
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.cell.2005.02.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000151064
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.febslet.2008.08.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031968140
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/s0002-9440(10)61254-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017146762
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/s0002-9440(10)63105-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013215860
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1056/nejmoa1404401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053072878
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1073/pnas.0307825100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014959706
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1073/pnas.89.22.10940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050592984
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1073/pnas.90.20.9678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037360856
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1073/pnas.93.26.15457 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034207954
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1074/jbc.270.32.19173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001830564
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1074/jbc.m110.161794 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020003733
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1074/jbc.m610051200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004744496
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1093/brain/awp196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001056335
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1126/science.1118829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062452678
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1128/jvi.01544-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022365860
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1371/journal.pone.0008765 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049300911
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1523/jneurosci.2467-05.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038193081
211 rdf:type schema:CreativeWork
212 https://doi.org/10.3201/eid1109.050371 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027331931
213 rdf:type schema:CreativeWork
214 https://doi.org/10.3201/eid1502.081173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045856525
215 rdf:type schema:CreativeWork
216 https://doi.org/10.3201/eid1801.110589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019361504
217 rdf:type schema:CreativeWork
218 https://doi.org/10.3201/eid2012.140548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040778276
219 rdf:type schema:CreativeWork
220 https://doi.org/10.3201/eid2306.161734 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084953056
221 rdf:type schema:CreativeWork
222 https://doi.org/10.3390/pathogens6040060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1093007208
223 rdf:type schema:CreativeWork
224 https://www.grid.ac/institutes/grid.267308.8 schema:alternateName The University of Texas Health Science Center at Houston
225 schema:name University of Texas Health Science Center, Department of Neurology, Houston, Texas, USA
226 rdf:type schema:Organization
227 https://www.grid.ac/institutes/grid.417894.7 schema:alternateName Istituto Neurologico Carlo Besta
228 schema:name Fondazione IRCCS Istituto Neurologico Carlo Besta, Neurology 5 and Neuropathology Unit, Milano, Italy
229 rdf:type schema:Organization
230 https://www.grid.ac/institutes/grid.67105.35 schema:alternateName Case Western Reserve University
231 schema:name Case Western Reserve University, Department of Pathology, Cleveland, Ohio, USA
232 Case Western Reserve University, National Prion Disease Pathology Surveillance Center and Departments of Neurology and Psychiatry, Cleveland, Ohio, USA
233 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...