Leishmania donovani 90 kD Heat Shock Protein – Impact of Phosphosites on Parasite Fitness, Infectivity and Casein Kinase Affinity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Antje Hombach-Barrigah, Katharina Bartsch, Despina Smirlis, Heidi Rosenqvist, Andrea MacDonald, Florent Dingli, Damarys Loew, Gerald F. Späth, Najma Rachidi, Martin Wiese, Joachim Clos

ABSTRACT

Leishmania parasites are thought to control protein activity at the post-translational level, e.g. by protein phosphorylation. In the pathogenic amastigote, the mammalian stage of Leishmania parasites, heat shock proteins show increased phosphorylation, indicating a role in stage-specific signal transduction. Here we investigate the impact of phosphosites in the L. donovani heat shock protein 90. Using a chemical knock-down/genetic complementation approach, we mutated 11 confirmed or presumed phosphorylation sites and assessed the impact on overall fitness, morphology and in vitro infectivity. Most phosphosite mutations affected the growth and morphology of promastigotes in vitro, but with one exception, none of the phosphorylation site mutants had a selective impact on the in vitro infection of macrophages. Surprisingly, aspartate replacements mimicking the negative charge of phosphorylated serines or threonines had mostly negative impacts on viability and infectivity. HSP90 is a substrate for casein kinase 1.2-catalysed phosphorylation in vitro. While several putative phosphosite mutations abrogated casein kinase 1.2 activity on HSP90, only Ser289 could be identified as casein kinase target by mass spectrometry. In summary, our data show HSP90 as a downstream client of phosphorylation-mediated signalling in an organism that depends on post-transcriptional gene regulation. More... »

PAGES

5074

References to SciGraph publications

  • 1998. Antibiotic radicicol binds to the N-terminal domain of Hsp90 and shares important biologic activities with geldanamycin. in CELL STRESS AND CHAPERONES
  • 2016-10. A versatile qPCR assay to quantify trypanosomatidic infections of host cells and tissues in MEDICAL MICROBIOLOGY AND IMMUNOLOGY
  • 2005-12. Stage-specific expression of the mitochondrial co-chaperonin of Leishmania donovani, CPN10 in KINETOPLASTID BIOLOGY AND DISEASE
  • 2011-01. Mixed Hsp90–cochaperone complexes are important for the progression of the reaction cycle in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 1991-07. Post-transcriptional regulation by light of the biosynthesis ofEuglena ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit in PLANT MOLECULAR BIOLOGY
  • 2017-12. MAPK1 of Leishmania donovani interacts and phosphorylates HSP70 and HSP90 subunits of foldosome complex in SCIENTIFIC REPORTS
  • 2009-01. Plasticity of the Hsp90 chaperone machine in divergent eukaryotic organisms in CELL STRESS AND CHAPERONES
  • 2017-09. Hsp90 inhibitors radicicol and geldanamycin have opposing effects on Leishmania Aha1-dependent proliferation in CELL STRESS AND CHAPERONES
  • 2005-10. HSP90 and the chaperoning of cancer in NATURE REVIEWS CANCER
  • 2014-12. An inhibitor-driven study for enhancing the selectivity of indirubin derivatives towards leishmanial Glycogen Synthase Kinase-3 over leishmanial cdc2-related protein kinase 3 in FILARIA JOURNAL
  • 1998-11. Hsp90 as a capacitor for morphological evolution in NATURE
  • 2013-06. C-terminal phosphorylation of Hsp70 and Hsp90 regulates alternate binding to co-chaperones CHIP and HOP to determine cellular protein folding/degradation balances in ONCOGENE
  • 2008-02. Proteomic analysis of the secretome of Leishmania donovani in GENOME BIOLOGY
  • 2015-07. Leishmania donovani P23 protects parasites against HSP90 inhibitor-mediated growth arrest in CELL STRESS AND CHAPERONES
  • 2010-07. The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability in CELL STRESS AND CHAPERONES
  • 2006-10. A probability-based approach for high-throughput protein phosphorylation analysis and site localization in NATURE BIOTECHNOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 2017-05-24. An Hsp90 co-chaperone protein in yeast is functionally replaced by site-specific posttranslational modification in humans in NATURE COMMUNICATIONS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-41640-0

    DOI

    http://dx.doi.org/10.1038/s41598-019-41640-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112971430

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30911045


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