Leishmania donovani 90 kD Heat Shock Protein – Impact of Phosphosites on Parasite Fitness, Infectivity and Casein Kinase Affinity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Antje Hombach-Barrigah, Katharina Bartsch, Despina Smirlis, Heidi Rosenqvist, Andrea MacDonald, Florent Dingli, Damarys Loew, Gerald F. Späth, Najma Rachidi, Martin Wiese, Joachim Clos

ABSTRACT

Leishmania parasites are thought to control protein activity at the post-translational level, e.g. by protein phosphorylation. In the pathogenic amastigote, the mammalian stage of Leishmania parasites, heat shock proteins show increased phosphorylation, indicating a role in stage-specific signal transduction. Here we investigate the impact of phosphosites in the L. donovani heat shock protein 90. Using a chemical knock-down/genetic complementation approach, we mutated 11 confirmed or presumed phosphorylation sites and assessed the impact on overall fitness, morphology and in vitro infectivity. Most phosphosite mutations affected the growth and morphology of promastigotes in vitro, but with one exception, none of the phosphorylation site mutants had a selective impact on the in vitro infection of macrophages. Surprisingly, aspartate replacements mimicking the negative charge of phosphorylated serines or threonines had mostly negative impacts on viability and infectivity. HSP90 is a substrate for casein kinase 1.2-catalysed phosphorylation in vitro. While several putative phosphosite mutations abrogated casein kinase 1.2 activity on HSP90, only Ser289 could be identified as casein kinase target by mass spectrometry. In summary, our data show HSP90 as a downstream client of phosphorylation-mediated signalling in an organism that depends on post-transcriptional gene regulation. More... »

PAGES

5074

References to SciGraph publications

  • 1998. Antibiotic radicicol binds to the N-terminal domain of Hsp90 and shares important biologic activities with geldanamycin. in CELL STRESS AND CHAPERONES
  • 2016-10. A versatile qPCR assay to quantify trypanosomatidic infections of host cells and tissues in MEDICAL MICROBIOLOGY AND IMMUNOLOGY
  • 2005-12. Stage-specific expression of the mitochondrial co-chaperonin of Leishmania donovani, CPN10 in KINETOPLASTID BIOLOGY AND DISEASE
  • 2011-01. Mixed Hsp90–cochaperone complexes are important for the progression of the reaction cycle in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 1991-07. Post-transcriptional regulation by light of the biosynthesis ofEuglena ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit in PLANT MOLECULAR BIOLOGY
  • 2017-12. MAPK1 of Leishmania donovani interacts and phosphorylates HSP70 and HSP90 subunits of foldosome complex in SCIENTIFIC REPORTS
  • 2009-01. Plasticity of the Hsp90 chaperone machine in divergent eukaryotic organisms in CELL STRESS AND CHAPERONES
  • 2017-09. Hsp90 inhibitors radicicol and geldanamycin have opposing effects on Leishmania Aha1-dependent proliferation in CELL STRESS AND CHAPERONES
  • 2005-10. HSP90 and the chaperoning of cancer in NATURE REVIEWS CANCER
  • 2014-12. An inhibitor-driven study for enhancing the selectivity of indirubin derivatives towards leishmanial Glycogen Synthase Kinase-3 over leishmanial cdc2-related protein kinase 3 in FILARIA JOURNAL
  • 1998-11. Hsp90 as a capacitor for morphological evolution in NATURE
  • 2013-06. C-terminal phosphorylation of Hsp70 and Hsp90 regulates alternate binding to co-chaperones CHIP and HOP to determine cellular protein folding/degradation balances in ONCOGENE
  • 2008-02. Proteomic analysis of the secretome of Leishmania donovani in GENOME BIOLOGY
  • 2015-07. Leishmania donovani P23 protects parasites against HSP90 inhibitor-mediated growth arrest in CELL STRESS AND CHAPERONES
  • 2010-07. The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability in CELL STRESS AND CHAPERONES
  • 2006-10. A probability-based approach for high-throughput protein phosphorylation analysis and site localization in NATURE BIOTECHNOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 2017-05-24. An Hsp90 co-chaperone protein in yeast is functionally replaced by site-specific posttranslational modification in humans in NATURE COMMUNICATIONS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-41640-0

    DOI

    http://dx.doi.org/10.1038/s41598-019-41640-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112971430

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30911045


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Bernhard Nocht Institute for Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.424065.1", 
              "name": [
                "Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hombach-Barrigah", 
            "givenName": "Antje", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Bernhard Nocht Institute for Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.424065.1", 
              "name": [
                "Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bartsch", 
            "givenName": "Katharina", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Pasteur Hellenic Institute", 
              "id": "https://www.grid.ac/institutes/grid.418497.7", 
              "name": [
                "Institut Pasteur and Institut National de Sant\u00e9 et Recherche M\u00e9dicale INSERM U1201, Unit\u00e9 de Parasitologie Mol\u00e9culaire et Signalisation, Paris, France", 
                "Hellenic Pasteur Institute, Athens, Greece"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Smirlis", 
            "givenName": "Despina", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Novo Nordisk (Denmark)", 
              "id": "https://www.grid.ac/institutes/grid.425956.9", 
              "name": [
                "Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS) University of Strathclyde, Glasgow, Scotland, UK", 
                "Novo Nordisk A/S, Gentofte, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rosenqvist", 
            "givenName": "Heidi", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Bernhard Nocht Institute for Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.424065.1", 
              "name": [
                "Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "MacDonald", 
            "givenName": "Andrea", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute Curie", 
              "id": "https://www.grid.ac/institutes/grid.418596.7", 
              "name": [
                "Laboratoire de Spectrom\u00e9trie de Masse Prot\u00e9omique, Centre de Recherche, Institut Curie, PSL Research University, Paris, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dingli", 
            "givenName": "Florent", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute Curie", 
              "id": "https://www.grid.ac/institutes/grid.418596.7", 
              "name": [
                "Laboratoire de Spectrom\u00e9trie de Masse Prot\u00e9omique, Centre de Recherche, Institut Curie, PSL Research University, Paris, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Loew", 
            "givenName": "Damarys", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institut Pasteur", 
              "id": "https://www.grid.ac/institutes/grid.428999.7", 
              "name": [
                "Institut Pasteur and Institut National de Sant\u00e9 et Recherche M\u00e9dicale INSERM U1201, Unit\u00e9 de Parasitologie Mol\u00e9culaire et Signalisation, Paris, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sp\u00e4th", 
            "givenName": "Gerald F.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institut Pasteur", 
              "id": "https://www.grid.ac/institutes/grid.428999.7", 
              "name": [
                "Institut Pasteur and Institut National de Sant\u00e9 et Recherche M\u00e9dicale INSERM U1201, Unit\u00e9 de Parasitologie Mol\u00e9culaire et Signalisation, Paris, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rachidi", 
            "givenName": "Najma", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Strathclyde", 
              "id": "https://www.grid.ac/institutes/grid.11984.35", 
              "name": [
                "Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS) University of Strathclyde, Glasgow, Scotland, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wiese", 
            "givenName": "Martin", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Bernhard Nocht Institute for Tropical Medicine", 
              "id": "https://www.grid.ac/institutes/grid.424065.1", 
              "name": [
                "Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Clos", 
            "givenName": "Joachim", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.18632/oncotarget.272", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000288053"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0914768107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000919812"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0166-6851(97)00115-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000970383"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0166-6851(95)00012-p", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001509947"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/cmi.12057", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002585099"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-4894(87)90138-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003880820"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/emboj/21.8.1881", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003976707"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/rcm.3254", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004142951"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12192-008-0058-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004456064", 
              "https://doi.org/10.1007/s12192-008-0058-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2011.02.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004557204"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/emboj/18.3.754", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004649322"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.18.8324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004811461"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mbio.00861-15", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006175019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/cmi.12456", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007167507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.00478-09", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008761234"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/cmi.12440", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009160834"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00036807", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009374520", 
              "https://doi.org/10.1007/bf00036807"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00036807", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009374520", 
              "https://doi.org/10.1007/bf00036807"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1716", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009936643", 
              "https://doi.org/10.1038/nrc1716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1716", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009936643", 
              "https://doi.org/10.1038/nrc1716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc1716", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009936643", 
              "https://doi.org/10.1038/nrc1716"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/713803539", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009971385"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m211309200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010209929"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/227680a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010419937", 
              "https://doi.org/10.1038/227680a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1096/fj.07-9254com", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010960467"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1214/aoms/1177730491", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011120195"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1240", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011188595", 
              "https://doi.org/10.1038/nbt1240"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1240", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011188595", 
              "https://doi.org/10.1038/nbt1240"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/prep.1994.1020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012331039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0167-4781(00)00028-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012766847"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jsb.2011.05.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014339311"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12192-015-0595-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014672812", 
              "https://doi.org/10.1007/s12192-015-0595-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pmic.200600784", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014894973"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamcr.2011.07.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015056583"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-5793(91)81320-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015323857"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.02188-05", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016574544"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.ge.22.120188.003215", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016656710"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj3100225", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017004345"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj3100225", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017004345"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-2952(98)00120-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017870116"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12192-009-0160-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019645921", 
              "https://doi.org/10.1007/s12192-009-0160-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12192-009-0160-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019645921", 
              "https://doi.org/10.1007/s12192-009-0160-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1475-9292-4-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019789908", 
              "https://doi.org/10.1186/1475-9292-4-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pmic.201100120", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020516957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aac.02022-13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020797501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.96.1.109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021542959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj3550609", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021636971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj3550609", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021636971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/ec.1.6.936-943.2002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021784811"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1096/fj.10-157529", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022583769"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/jcs.056465", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022596813"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijpara.2008.03.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022634439"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0968-0004(00)01712-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024082635"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3389/fonc.2014.00096", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025200282"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pmic.200700697", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026744406"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/mcp.m114.037705", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028231606"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00430-016-0460-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028469012", 
              "https://doi.org/10.1007/s00430-016-0460-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00430-016-0460-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028469012", 
              "https://doi.org/10.1007/s00430-016-0460-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.biochem.75.103004.142738", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029045805"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pmic.200300462", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030125534"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4049/jimmunol.1000541", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030785838"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.12.11.3307", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031552621"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.18.9.4949", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032214543"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/24550", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032477985", 
              "https://doi.org/10.1038/24550"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1756-3305-7-234", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032867046", 
              "https://doi.org/10.1186/1756-3305-7-234"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0035671", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033992319"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijpara.2008.10.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034655707"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molbiopara.2010.12.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036473150"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcb.22019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036490866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/emboj/17.9.2619", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036916870"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/jeu.12329", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037168633"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m105562200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037780394"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/gb-2008-9-2-r35", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037866253", 
              "https://doi.org/10.1186/gb-2008-9-2-r35"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.15.7.3917", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038524546"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2013.12.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038564406"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.gene.2015.06.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038799659"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0020-7519(02)00067-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039316245"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1733909100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041212811"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.473902", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042166472"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aac.48.8.3033-3042.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043107432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/ec.2.5.830-840.2003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043320976"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molbiopara.2005.02.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044347005"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2012.05.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044595837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/jcs.00905", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045542395"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijpara.2007.04.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046864901"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/mmi.12639", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047731695"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1965", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048382613", 
              "https://doi.org/10.1038/nsmb.1965"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1965", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048382613", 
              "https://doi.org/10.1038/nsmb.1965"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/onc.2012.314", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049708287", 
              "https://doi.org/10.1038/onc.2012.314"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(94)90003-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050306074"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijpara.2009.04.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050682505"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1155/2010/525241", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050860695"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1242/jcs.157297", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051668721"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/mmi.13375", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051854341"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1248/bpb.31.193", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052804686"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/pr900721e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056295100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.273.11.6488", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1058213295"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.6701528", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062640007"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1210/mend.10.6.8776730", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064331305"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1210/mend.13.9.0339", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1064331877"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1379/1466-1268(1998)003<0100:arbttn>2.3.co;2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1067173635", 
              "https://doi.org/10.1379/1466-1268(1998)003<0100:arbttn>2.3.co;2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4161/cc.9.12.12054", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072300863"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.4172/jpb.1000163", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1072344138"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074677057", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080266452", 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12192-017-0800-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085090649", 
              "https://doi.org/10.1007/s12192-017-0800-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12192-017-0800-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085090649", 
              "https://doi.org/10.1007/s12192-017-0800-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncomms15328", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085577811", 
              "https://doi.org/10.1038/ncomms15328"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-09725-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091356638", 
              "https://doi.org/10.1038/s41598-017-09725-w"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-09725-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091356638", 
              "https://doi.org/10.1038/s41598-017-09725-w"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/msystems.00214-18", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110043934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/msystems.00214-18", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110043934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/msystems.00214-18", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110043934"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-12", 
        "datePublishedReg": "2019-12-01", 
        "description": "Leishmania parasites are thought to control protein activity at the post-translational level, e.g. by protein phosphorylation. In the pathogenic amastigote, the mammalian stage of Leishmania parasites, heat shock proteins show increased phosphorylation, indicating a role in stage-specific signal transduction. Here we investigate the impact of phosphosites in the L. donovani heat shock protein 90. Using a chemical knock-down/genetic complementation approach, we mutated 11 confirmed or presumed phosphorylation sites and assessed the impact on overall fitness, morphology and in vitro infectivity. Most phosphosite mutations affected the growth and morphology of promastigotes in vitro, but with one exception, none of the phosphorylation site mutants had a selective impact on the in vitro infection of macrophages. Surprisingly, aspartate replacements mimicking the negative charge of phosphorylated serines or threonines had mostly negative impacts on viability and infectivity. HSP90 is a substrate for casein kinase 1.2-catalysed phosphorylation in vitro. While several putative phosphosite mutations abrogated casein kinase 1.2 activity on HSP90, only Ser289 could be identified as casein kinase target by mass spectrometry. In summary, our data show HSP90 as a downstream client of phosphorylation-mediated signalling in an organism that depends on post-transcriptional gene regulation.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/s41598-019-41640-0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.4528983", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "name": "Leishmania donovani 90 kD Heat Shock Protein \u2013 Impact of Phosphosites on Parasite Fitness, Infectivity and Casein Kinase Affinity", 
        "pagination": "5074", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "eb6728c628e2d8a5573c763db9e80958c3a898ee006587cd52199d97b65b4ed9"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30911045"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101563288"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-019-41640-0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1112971430"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-019-41640-0", 
          "https://app.dimensions.ai/details/publication/pub.1112971430"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:59", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130823_00000006.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/s41598-019-41640-0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41640-0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41640-0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41640-0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41640-0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    462 TRIPLES      21 PREDICATES      129 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-019-41640-0 schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author Na7a0a9f8ac944fba95e3115e78bdc317
    4 schema:citation sg:pub.10.1007/bf00036807
    5 sg:pub.10.1007/s00430-016-0460-3
    6 sg:pub.10.1007/s12192-008-0058-9
    7 sg:pub.10.1007/s12192-009-0160-7
    8 sg:pub.10.1007/s12192-015-0595-y
    9 sg:pub.10.1007/s12192-017-0800-2
    10 sg:pub.10.1038/227680a0
    11 sg:pub.10.1038/24550
    12 sg:pub.10.1038/nbt1240
    13 sg:pub.10.1038/ncomms15328
    14 sg:pub.10.1038/nrc1716
    15 sg:pub.10.1038/nsmb.1965
    16 sg:pub.10.1038/onc.2012.314
    17 sg:pub.10.1038/s41598-017-09725-w
    18 sg:pub.10.1186/1475-9292-4-3
    19 sg:pub.10.1186/1756-3305-7-234
    20 sg:pub.10.1186/gb-2008-9-2-r35
    21 sg:pub.10.1379/1466-1268(1998)003<0100:arbttn>2.3.co;2
    22 https://app.dimensions.ai/details/publication/pub.1074677057
    23 https://app.dimensions.ai/details/publication/pub.1080266452
    24 https://doi.org/10.1002/jcb.22019
    25 https://doi.org/10.1002/pmic.200300462
    26 https://doi.org/10.1002/pmic.200600784
    27 https://doi.org/10.1002/pmic.200700697
    28 https://doi.org/10.1002/pmic.201100120
    29 https://doi.org/10.1002/rcm.3254
    30 https://doi.org/10.1006/prep.1994.1020
    31 https://doi.org/10.1016/0014-4894(87)90138-x
    32 https://doi.org/10.1016/0014-5793(91)81320-8
    33 https://doi.org/10.1016/0092-8674(94)90003-5
    34 https://doi.org/10.1016/0166-6851(95)00012-p
    35 https://doi.org/10.1016/j.bbamcr.2011.07.018
    36 https://doi.org/10.1016/j.gene.2015.06.018
    37 https://doi.org/10.1016/j.ijpara.2007.04.008
    38 https://doi.org/10.1016/j.ijpara.2008.03.005
    39 https://doi.org/10.1016/j.ijpara.2008.10.009
    40 https://doi.org/10.1016/j.ijpara.2009.04.005
    41 https://doi.org/10.1016/j.jsb.2011.05.009
    42 https://doi.org/10.1016/j.molbiopara.2005.02.004
    43 https://doi.org/10.1016/j.molbiopara.2010.12.009
    44 https://doi.org/10.1016/j.molcel.2011.02.011
    45 https://doi.org/10.1016/j.molcel.2012.05.015
    46 https://doi.org/10.1016/j.molcel.2013.12.007
    47 https://doi.org/10.1016/s0006-2952(98)00120-8
    48 https://doi.org/10.1016/s0020-7519(02)00067-x
    49 https://doi.org/10.1016/s0166-6851(97)00115-1
    50 https://doi.org/10.1016/s0167-4781(00)00028-2
    51 https://doi.org/10.1016/s0968-0004(00)01712-6
    52 https://doi.org/10.1021/pr900721e
    53 https://doi.org/10.1042/bj3100225
    54 https://doi.org/10.1042/bj3550609
    55 https://doi.org/10.1073/pnas.0914768107
    56 https://doi.org/10.1073/pnas.1733909100
    57 https://doi.org/10.1073/pnas.91.18.8324
    58 https://doi.org/10.1073/pnas.96.1.109
    59 https://doi.org/10.1074/jbc.273.11.6488
    60 https://doi.org/10.1074/jbc.m105562200
    61 https://doi.org/10.1074/jbc.m211309200
    62 https://doi.org/10.1074/mcp.m114.037705
    63 https://doi.org/10.1080/713803539
    64 https://doi.org/10.1091/mbc.12.11.3307
    65 https://doi.org/10.1093/emboj/17.9.2619
    66 https://doi.org/10.1093/emboj/18.3.754
    67 https://doi.org/10.1093/emboj/21.8.1881
    68 https://doi.org/10.1096/fj.07-9254com
    69 https://doi.org/10.1096/fj.10-157529
    70 https://doi.org/10.1101/gr.473902
    71 https://doi.org/10.1111/cmi.12057
    72 https://doi.org/10.1111/cmi.12440
    73 https://doi.org/10.1111/cmi.12456
    74 https://doi.org/10.1111/jeu.12329
    75 https://doi.org/10.1111/mmi.12639
    76 https://doi.org/10.1111/mmi.13375
    77 https://doi.org/10.1126/science.6701528
    78 https://doi.org/10.1128/aac.02022-13
    79 https://doi.org/10.1128/aac.48.8.3033-3042.2004
    80 https://doi.org/10.1128/ec.1.6.936-943.2002
    81 https://doi.org/10.1128/ec.2.5.830-840.2003
    82 https://doi.org/10.1128/mbio.00861-15
    83 https://doi.org/10.1128/mcb.00478-09
    84 https://doi.org/10.1128/mcb.02188-05
    85 https://doi.org/10.1128/mcb.15.7.3917
    86 https://doi.org/10.1128/mcb.18.9.4949
    87 https://doi.org/10.1128/msystems.00214-18
    88 https://doi.org/10.1146/annurev.biochem.75.103004.142738
    89 https://doi.org/10.1146/annurev.ge.22.120188.003215
    90 https://doi.org/10.1155/2010/525241
    91 https://doi.org/10.1210/mend.10.6.8776730
    92 https://doi.org/10.1210/mend.13.9.0339
    93 https://doi.org/10.1214/aoms/1177730491
    94 https://doi.org/10.1242/jcs.00905
    95 https://doi.org/10.1242/jcs.056465
    96 https://doi.org/10.1242/jcs.157297
    97 https://doi.org/10.1248/bpb.31.193
    98 https://doi.org/10.1371/journal.pone.0035671
    99 https://doi.org/10.18632/oncotarget.272
    100 https://doi.org/10.3389/fonc.2014.00096
    101 https://doi.org/10.4049/jimmunol.1000541
    102 https://doi.org/10.4161/cc.9.12.12054
    103 https://doi.org/10.4172/jpb.1000163
    104 schema:datePublished 2019-12
    105 schema:datePublishedReg 2019-12-01
    106 schema:description Leishmania parasites are thought to control protein activity at the post-translational level, e.g. by protein phosphorylation. In the pathogenic amastigote, the mammalian stage of Leishmania parasites, heat shock proteins show increased phosphorylation, indicating a role in stage-specific signal transduction. Here we investigate the impact of phosphosites in the L. donovani heat shock protein 90. Using a chemical knock-down/genetic complementation approach, we mutated 11 confirmed or presumed phosphorylation sites and assessed the impact on overall fitness, morphology and in vitro infectivity. Most phosphosite mutations affected the growth and morphology of promastigotes in vitro, but with one exception, none of the phosphorylation site mutants had a selective impact on the in vitro infection of macrophages. Surprisingly, aspartate replacements mimicking the negative charge of phosphorylated serines or threonines had mostly negative impacts on viability and infectivity. HSP90 is a substrate for casein kinase 1.2-catalysed phosphorylation in vitro. While several putative phosphosite mutations abrogated casein kinase 1.2 activity on HSP90, only Ser<sub>289</sub> could be identified as casein kinase target by mass spectrometry. In summary, our data show HSP90 as a downstream client of phosphorylation-mediated signalling in an organism that depends on post-transcriptional gene regulation.
    107 schema:genre research_article
    108 schema:inLanguage en
    109 schema:isAccessibleForFree true
    110 schema:isPartOf N8a92346ba7bf4309b913a5efce132ba7
    111 Nd573c72e21f24510bdd827fd7bd7bdaa
    112 sg:journal.1045337
    113 schema:name Leishmania donovani 90 kD Heat Shock Protein – Impact of Phosphosites on Parasite Fitness, Infectivity and Casein Kinase Affinity
    114 schema:pagination 5074
    115 schema:productId N34a9268bbff4421c9d5da11ffb44f903
    116 N3f89ce56f5ec43878cd77bb95fd470f9
    117 N8144c76c61bf40feb06ad3605497b267
    118 Nd913f4905bf941d3830f37db858de2f6
    119 Nfb83eccd1df14772ad59a44456107acc
    120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112971430
    121 https://doi.org/10.1038/s41598-019-41640-0
    122 schema:sdDatePublished 2019-04-11T13:59
    123 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    124 schema:sdPublisher N4da975f4fdac423b936f23e69d9416c2
    125 schema:url https://www.nature.com/articles/s41598-019-41640-0
    126 sgo:license sg:explorer/license/
    127 sgo:sdDataset articles
    128 rdf:type schema:ScholarlyArticle
    129 N0fd19bf7a2b74ad68d7b9b5ae584ab3d schema:affiliation https://www.grid.ac/institutes/grid.425956.9
    130 schema:familyName Rosenqvist
    131 schema:givenName Heidi
    132 rdf:type schema:Person
    133 N2731918eb4374d35b4fdafa21818e19e rdf:first Nca662ef28b09437b9bdb07259f92406d
    134 rdf:rest Ne2cb07a1eb9c4ca9b58737a5f720dbc9
    135 N2e3ac3b14eb04f1e8339773b83cccc4f schema:affiliation https://www.grid.ac/institutes/grid.418596.7
    136 schema:familyName Dingli
    137 schema:givenName Florent
    138 rdf:type schema:Person
    139 N2e6319efb21e4c4287533b826d24cc87 rdf:first N525f5c553222485cb0d22b40a9f5ba87
    140 rdf:rest Nf95f38637ba64dae8c4029b1f38c5865
    141 N30d6fbc028594e8399b81f3b04d88d56 schema:affiliation https://www.grid.ac/institutes/grid.11984.35
    142 schema:familyName Wiese
    143 schema:givenName Martin
    144 rdf:type schema:Person
    145 N34a9268bbff4421c9d5da11ffb44f903 schema:name readcube_id
    146 schema:value eb6728c628e2d8a5573c763db9e80958c3a898ee006587cd52199d97b65b4ed9
    147 rdf:type schema:PropertyValue
    148 N3f89ce56f5ec43878cd77bb95fd470f9 schema:name doi
    149 schema:value 10.1038/s41598-019-41640-0
    150 rdf:type schema:PropertyValue
    151 N4da975f4fdac423b936f23e69d9416c2 schema:name Springer Nature - SN SciGraph project
    152 rdf:type schema:Organization
    153 N525f5c553222485cb0d22b40a9f5ba87 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
    154 schema:familyName Rachidi
    155 schema:givenName Najma
    156 rdf:type schema:Person
    157 N53ce146deb7f4fa08e2fb9c7b7ae651f rdf:first N0fd19bf7a2b74ad68d7b9b5ae584ab3d
    158 rdf:rest Nfa5c5600f0224d84b0f1954b378881dc
    159 N563ff17a2603459c9f038090d4ae94aa schema:affiliation https://www.grid.ac/institutes/grid.424065.1
    160 schema:familyName Hombach-Barrigah
    161 schema:givenName Antje
    162 rdf:type schema:Person
    163 N569e429f45014d9994099b9b633a2eb4 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
    164 schema:familyName Späth
    165 schema:givenName Gerald F.
    166 rdf:type schema:Person
    167 N5de926f9b72d49efa1f455f5acd16179 schema:affiliation https://www.grid.ac/institutes/grid.424065.1
    168 schema:familyName Clos
    169 schema:givenName Joachim
    170 rdf:type schema:Person
    171 N671d11c6748c4390ace350ff41f200c1 rdf:first Nd4b9cf1565e646f595d3654e47251c21
    172 rdf:rest Neb30be562b3049558f3063124e3ff433
    173 N8144c76c61bf40feb06ad3605497b267 schema:name nlm_unique_id
    174 schema:value 101563288
    175 rdf:type schema:PropertyValue
    176 N8a92346ba7bf4309b913a5efce132ba7 schema:issueNumber 1
    177 rdf:type schema:PublicationIssue
    178 Na7a0a9f8ac944fba95e3115e78bdc317 rdf:first N563ff17a2603459c9f038090d4ae94aa
    179 rdf:rest N2731918eb4374d35b4fdafa21818e19e
    180 Nb8f84c998ec64b85ada57b61fc1ddb9d schema:affiliation https://www.grid.ac/institutes/grid.424065.1
    181 schema:familyName MacDonald
    182 schema:givenName Andrea
    183 rdf:type schema:Person
    184 Nc846907253a149caa162c751acefbf00 rdf:first N2e3ac3b14eb04f1e8339773b83cccc4f
    185 rdf:rest N671d11c6748c4390ace350ff41f200c1
    186 Nca662ef28b09437b9bdb07259f92406d schema:affiliation https://www.grid.ac/institutes/grid.424065.1
    187 schema:familyName Bartsch
    188 schema:givenName Katharina
    189 rdf:type schema:Person
    190 Nd4b9cf1565e646f595d3654e47251c21 schema:affiliation https://www.grid.ac/institutes/grid.418596.7
    191 schema:familyName Loew
    192 schema:givenName Damarys
    193 rdf:type schema:Person
    194 Nd573c72e21f24510bdd827fd7bd7bdaa schema:volumeNumber 9
    195 rdf:type schema:PublicationVolume
    196 Nd746658a14534ec9997a292327cf3c3d schema:affiliation https://www.grid.ac/institutes/grid.418497.7
    197 schema:familyName Smirlis
    198 schema:givenName Despina
    199 rdf:type schema:Person
    200 Nd913f4905bf941d3830f37db858de2f6 schema:name pubmed_id
    201 schema:value 30911045
    202 rdf:type schema:PropertyValue
    203 Ne2cb07a1eb9c4ca9b58737a5f720dbc9 rdf:first Nd746658a14534ec9997a292327cf3c3d
    204 rdf:rest N53ce146deb7f4fa08e2fb9c7b7ae651f
    205 Ne3ca1ac6a27a4dab80ccf2d92278357d rdf:first N5de926f9b72d49efa1f455f5acd16179
    206 rdf:rest rdf:nil
    207 Neb30be562b3049558f3063124e3ff433 rdf:first N569e429f45014d9994099b9b633a2eb4
    208 rdf:rest N2e6319efb21e4c4287533b826d24cc87
    209 Nf95f38637ba64dae8c4029b1f38c5865 rdf:first N30d6fbc028594e8399b81f3b04d88d56
    210 rdf:rest Ne3ca1ac6a27a4dab80ccf2d92278357d
    211 Nfa5c5600f0224d84b0f1954b378881dc rdf:first Nb8f84c998ec64b85ada57b61fc1ddb9d
    212 rdf:rest Nc846907253a149caa162c751acefbf00
    213 Nfb83eccd1df14772ad59a44456107acc schema:name dimensions_id
    214 schema:value pub.1112971430
    215 rdf:type schema:PropertyValue
    216 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    217 schema:name Biological Sciences
    218 rdf:type schema:DefinedTerm
    219 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    220 schema:name Biochemistry and Cell Biology
    221 rdf:type schema:DefinedTerm
    222 sg:grant.4528983 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41640-0
    223 rdf:type schema:MonetaryGrant
    224 sg:journal.1045337 schema:issn 2045-2322
    225 schema:name Scientific Reports
    226 rdf:type schema:Periodical
    227 sg:pub.10.1007/bf00036807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009374520
    228 https://doi.org/10.1007/bf00036807
    229 rdf:type schema:CreativeWork
    230 sg:pub.10.1007/s00430-016-0460-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028469012
    231 https://doi.org/10.1007/s00430-016-0460-3
    232 rdf:type schema:CreativeWork
    233 sg:pub.10.1007/s12192-008-0058-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004456064
    234 https://doi.org/10.1007/s12192-008-0058-9
    235 rdf:type schema:CreativeWork
    236 sg:pub.10.1007/s12192-009-0160-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019645921
    237 https://doi.org/10.1007/s12192-009-0160-7
    238 rdf:type schema:CreativeWork
    239 sg:pub.10.1007/s12192-015-0595-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1014672812
    240 https://doi.org/10.1007/s12192-015-0595-y
    241 rdf:type schema:CreativeWork
    242 sg:pub.10.1007/s12192-017-0800-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085090649
    243 https://doi.org/10.1007/s12192-017-0800-2
    244 rdf:type schema:CreativeWork
    245 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
    246 https://doi.org/10.1038/227680a0
    247 rdf:type schema:CreativeWork
    248 sg:pub.10.1038/24550 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032477985
    249 https://doi.org/10.1038/24550
    250 rdf:type schema:CreativeWork
    251 sg:pub.10.1038/nbt1240 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011188595
    252 https://doi.org/10.1038/nbt1240
    253 rdf:type schema:CreativeWork
    254 sg:pub.10.1038/ncomms15328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085577811
    255 https://doi.org/10.1038/ncomms15328
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1038/nrc1716 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009936643
    258 https://doi.org/10.1038/nrc1716
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1038/nsmb.1965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048382613
    261 https://doi.org/10.1038/nsmb.1965
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1038/onc.2012.314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049708287
    264 https://doi.org/10.1038/onc.2012.314
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.1038/s41598-017-09725-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1091356638
    267 https://doi.org/10.1038/s41598-017-09725-w
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1186/1475-9292-4-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019789908
    270 https://doi.org/10.1186/1475-9292-4-3
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1186/1756-3305-7-234 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032867046
    273 https://doi.org/10.1186/1756-3305-7-234
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1186/gb-2008-9-2-r35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037866253
    276 https://doi.org/10.1186/gb-2008-9-2-r35
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1379/1466-1268(1998)003<0100:arbttn>2.3.co;2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1067173635
    279 https://doi.org/10.1379/1466-1268(1998)003<0100:arbttn>2.3.co;2
    280 rdf:type schema:CreativeWork
    281 https://app.dimensions.ai/details/publication/pub.1074677057 schema:CreativeWork
    282 https://app.dimensions.ai/details/publication/pub.1080266452 schema:CreativeWork
    283 https://doi.org/10.1002/jcb.22019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036490866
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1002/pmic.200300462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030125534
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1002/pmic.200600784 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014894973
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1002/pmic.200700697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026744406
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1002/pmic.201100120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020516957
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1002/rcm.3254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004142951
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1006/prep.1994.1020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012331039
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1016/0014-4894(87)90138-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003880820
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1016/0014-5793(91)81320-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015323857
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1016/0092-8674(94)90003-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050306074
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1016/0166-6851(95)00012-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1001509947
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1016/j.bbamcr.2011.07.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015056583
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.1016/j.gene.2015.06.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038799659
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.1016/j.ijpara.2007.04.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046864901
    310 rdf:type schema:CreativeWork
    311 https://doi.org/10.1016/j.ijpara.2008.03.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022634439
    312 rdf:type schema:CreativeWork
    313 https://doi.org/10.1016/j.ijpara.2008.10.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034655707
    314 rdf:type schema:CreativeWork
    315 https://doi.org/10.1016/j.ijpara.2009.04.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050682505
    316 rdf:type schema:CreativeWork
    317 https://doi.org/10.1016/j.jsb.2011.05.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014339311
    318 rdf:type schema:CreativeWork
    319 https://doi.org/10.1016/j.molbiopara.2005.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044347005
    320 rdf:type schema:CreativeWork
    321 https://doi.org/10.1016/j.molbiopara.2010.12.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036473150
    322 rdf:type schema:CreativeWork
    323 https://doi.org/10.1016/j.molcel.2011.02.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004557204
    324 rdf:type schema:CreativeWork
    325 https://doi.org/10.1016/j.molcel.2012.05.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044595837
    326 rdf:type schema:CreativeWork
    327 https://doi.org/10.1016/j.molcel.2013.12.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038564406
    328 rdf:type schema:CreativeWork
    329 https://doi.org/10.1016/s0006-2952(98)00120-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017870116
    330 rdf:type schema:CreativeWork
    331 https://doi.org/10.1016/s0020-7519(02)00067-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1039316245
    332 rdf:type schema:CreativeWork
    333 https://doi.org/10.1016/s0166-6851(97)00115-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000970383
    334 rdf:type schema:CreativeWork
    335 https://doi.org/10.1016/s0167-4781(00)00028-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012766847
    336 rdf:type schema:CreativeWork
    337 https://doi.org/10.1016/s0968-0004(00)01712-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024082635
    338 rdf:type schema:CreativeWork
    339 https://doi.org/10.1021/pr900721e schema:sameAs https://app.dimensions.ai/details/publication/pub.1056295100
    340 rdf:type schema:CreativeWork
    341 https://doi.org/10.1042/bj3100225 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017004345
    342 rdf:type schema:CreativeWork
    343 https://doi.org/10.1042/bj3550609 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021636971
    344 rdf:type schema:CreativeWork
    345 https://doi.org/10.1073/pnas.0914768107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000919812
    346 rdf:type schema:CreativeWork
    347 https://doi.org/10.1073/pnas.1733909100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041212811
    348 rdf:type schema:CreativeWork
    349 https://doi.org/10.1073/pnas.91.18.8324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004811461
    350 rdf:type schema:CreativeWork
    351 https://doi.org/10.1073/pnas.96.1.109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021542959
    352 rdf:type schema:CreativeWork
    353 https://doi.org/10.1074/jbc.273.11.6488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058213295
    354 rdf:type schema:CreativeWork
    355 https://doi.org/10.1074/jbc.m105562200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037780394
    356 rdf:type schema:CreativeWork
    357 https://doi.org/10.1074/jbc.m211309200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010209929
    358 rdf:type schema:CreativeWork
    359 https://doi.org/10.1074/mcp.m114.037705 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028231606
    360 rdf:type schema:CreativeWork
    361 https://doi.org/10.1080/713803539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009971385
    362 rdf:type schema:CreativeWork
    363 https://doi.org/10.1091/mbc.12.11.3307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031552621
    364 rdf:type schema:CreativeWork
    365 https://doi.org/10.1093/emboj/17.9.2619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036916870
    366 rdf:type schema:CreativeWork
    367 https://doi.org/10.1093/emboj/18.3.754 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004649322
    368 rdf:type schema:CreativeWork
    369 https://doi.org/10.1093/emboj/21.8.1881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003976707
    370 rdf:type schema:CreativeWork
    371 https://doi.org/10.1096/fj.07-9254com schema:sameAs https://app.dimensions.ai/details/publication/pub.1010960467
    372 rdf:type schema:CreativeWork
    373 https://doi.org/10.1096/fj.10-157529 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022583769
    374 rdf:type schema:CreativeWork
    375 https://doi.org/10.1101/gr.473902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042166472
    376 rdf:type schema:CreativeWork
    377 https://doi.org/10.1111/cmi.12057 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002585099
    378 rdf:type schema:CreativeWork
    379 https://doi.org/10.1111/cmi.12440 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009160834
    380 rdf:type schema:CreativeWork
    381 https://doi.org/10.1111/cmi.12456 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007167507
    382 rdf:type schema:CreativeWork
    383 https://doi.org/10.1111/jeu.12329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037168633
    384 rdf:type schema:CreativeWork
    385 https://doi.org/10.1111/mmi.12639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047731695
    386 rdf:type schema:CreativeWork
    387 https://doi.org/10.1111/mmi.13375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051854341
    388 rdf:type schema:CreativeWork
    389 https://doi.org/10.1126/science.6701528 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062640007
    390 rdf:type schema:CreativeWork
    391 https://doi.org/10.1128/aac.02022-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020797501
    392 rdf:type schema:CreativeWork
    393 https://doi.org/10.1128/aac.48.8.3033-3042.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043107432
    394 rdf:type schema:CreativeWork
    395 https://doi.org/10.1128/ec.1.6.936-943.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021784811
    396 rdf:type schema:CreativeWork
    397 https://doi.org/10.1128/ec.2.5.830-840.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043320976
    398 rdf:type schema:CreativeWork
    399 https://doi.org/10.1128/mbio.00861-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006175019
    400 rdf:type schema:CreativeWork
    401 https://doi.org/10.1128/mcb.00478-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008761234
    402 rdf:type schema:CreativeWork
    403 https://doi.org/10.1128/mcb.02188-05 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016574544
    404 rdf:type schema:CreativeWork
    405 https://doi.org/10.1128/mcb.15.7.3917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038524546
    406 rdf:type schema:CreativeWork
    407 https://doi.org/10.1128/mcb.18.9.4949 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032214543
    408 rdf:type schema:CreativeWork
    409 https://doi.org/10.1128/msystems.00214-18 schema:sameAs https://app.dimensions.ai/details/publication/pub.1110043934
    410 rdf:type schema:CreativeWork
    411 https://doi.org/10.1146/annurev.biochem.75.103004.142738 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029045805
    412 rdf:type schema:CreativeWork
    413 https://doi.org/10.1146/annurev.ge.22.120188.003215 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016656710
    414 rdf:type schema:CreativeWork
    415 https://doi.org/10.1155/2010/525241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050860695
    416 rdf:type schema:CreativeWork
    417 https://doi.org/10.1210/mend.10.6.8776730 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064331305
    418 rdf:type schema:CreativeWork
    419 https://doi.org/10.1210/mend.13.9.0339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064331877
    420 rdf:type schema:CreativeWork
    421 https://doi.org/10.1214/aoms/1177730491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011120195
    422 rdf:type schema:CreativeWork
    423 https://doi.org/10.1242/jcs.00905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045542395
    424 rdf:type schema:CreativeWork
    425 https://doi.org/10.1242/jcs.056465 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022596813
    426 rdf:type schema:CreativeWork
    427 https://doi.org/10.1242/jcs.157297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051668721
    428 rdf:type schema:CreativeWork
    429 https://doi.org/10.1248/bpb.31.193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052804686
    430 rdf:type schema:CreativeWork
    431 https://doi.org/10.1371/journal.pone.0035671 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033992319
    432 rdf:type schema:CreativeWork
    433 https://doi.org/10.18632/oncotarget.272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000288053
    434 rdf:type schema:CreativeWork
    435 https://doi.org/10.3389/fonc.2014.00096 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025200282
    436 rdf:type schema:CreativeWork
    437 https://doi.org/10.4049/jimmunol.1000541 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030785838
    438 rdf:type schema:CreativeWork
    439 https://doi.org/10.4161/cc.9.12.12054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072300863
    440 rdf:type schema:CreativeWork
    441 https://doi.org/10.4172/jpb.1000163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072344138
    442 rdf:type schema:CreativeWork
    443 https://www.grid.ac/institutes/grid.11984.35 schema:alternateName University of Strathclyde
    444 schema:name Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS) University of Strathclyde, Glasgow, Scotland, UK
    445 rdf:type schema:Organization
    446 https://www.grid.ac/institutes/grid.418497.7 schema:alternateName Pasteur Hellenic Institute
    447 schema:name Hellenic Pasteur Institute, Athens, Greece
    448 Institut Pasteur and Institut National de Santé et Recherche Médicale INSERM U1201, Unité de Parasitologie Moléculaire et Signalisation, Paris, France
    449 rdf:type schema:Organization
    450 https://www.grid.ac/institutes/grid.418596.7 schema:alternateName Institute Curie
    451 schema:name Laboratoire de Spectrométrie de Masse Protéomique, Centre de Recherche, Institut Curie, PSL Research University, Paris, France
    452 rdf:type schema:Organization
    453 https://www.grid.ac/institutes/grid.424065.1 schema:alternateName Bernhard Nocht Institute for Tropical Medicine
    454 schema:name Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany
    455 rdf:type schema:Organization
    456 https://www.grid.ac/institutes/grid.425956.9 schema:alternateName Novo Nordisk (Denmark)
    457 schema:name Novo Nordisk A/S, Gentofte, Denmark
    458 Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS) University of Strathclyde, Glasgow, Scotland, UK
    459 rdf:type schema:Organization
    460 https://www.grid.ac/institutes/grid.428999.7 schema:alternateName Institut Pasteur
    461 schema:name Institut Pasteur and Institut National de Santé et Recherche Médicale INSERM U1201, Unité de Parasitologie Moléculaire et Signalisation, Paris, France
    462 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...