An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Hui Li, Ye Huang, Zewen Wei, Wei Wang, Zhenjun Yang, Zicai Liang, Zhihong Li

ABSTRACT

Synthetic oligonucleotides (oligos) are important tools in the fields of molecular biology and genetic engineering. For applications requiring a large number of oligos with high concentration, it is critical to perform high throughput oligo synthesis and achieve high yield of each oligo. This study reports a microreactor chip for oligo synthesis. By incorporating silica beads in the microreactors, the surface area of the solid substrate for oligo synthesis increases significantly in each microreactor. These beads are fixed in the microreactors to withstand the flushing step in oligo synthesis. Compared to conventional synthesis methods, this design is able to avoid protocols to hold the beads and integrate more microreactors on a chip. An inkjet printer is utilized to deliver chemical reagents in the microreactors. To evaluate the feasibility of oligo synthesis using this proof-of-concept synthesizer, an oligo with six nucleotide units is successfully synthesized. More... »

PAGES

5058

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-41519-0

DOI

http://dx.doi.org/10.1038/s41598-019-41519-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112968718

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30911034


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