The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Sarah Waldie, Martine Moulin, Lionel Porcar, Harald Pichler, Gernot A. Strohmeier, Maximilian Skoda, V. Trevor Forsyth, Michael Haertlein, Selma Maric, Marité Cárdenas

ABSTRACT

The deuteration of biomolecules provides advanced opportunities for neutron scattering studies. For low resolution studies using techniques such as small-angle neutron scattering and neutron reflection, the level of deuteration of a sample can be varied to match the scattering length density of a specific D2O/H2O solvent mixture. This can be of major value in structural studies where specific regions of a complex system can be highlighted, and others rendered invisible. This is especially useful in analyses of the structure and dynamics of membrane components. In mammalian membranes, the presence of cholesterol is crucial in modulating the properties of lipids and in their interaction with proteins. Here, a protocol is described for the production of partially deuterated cholesterol which has a neutron scattering length density that matches that of 100% D2O solvent (hereby named matchout cholesterol). The level of deuteration was determined by mass spectrometry and nuclear magnetic resonance. The cholesterol match-point was verified experimentally using small angle neutron scattering. The matchout cholesterol was used to investigate the incorporation of cholesterol in various phosphatidylcholine supported lipid bilayers by neutron reflectometry. The study included both saturated and unsaturated lipids, as well as lipids with varying chain lengths. It was found that cholesterol is distributed asymmetrically within the bilayer, positioned closer to the headgroups of the lipids than to the middle of the tail core, regardless of the phosphatidylcholine species. More... »

PAGES

5118

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-41439-z

DOI

http://dx.doi.org/10.1038/s41598-019-41439-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112988342

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30914734


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Other Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Malm\u00f6 University", 
          "id": "https://www.grid.ac/institutes/grid.32995.34", 
          "name": [
            "Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France", 
            "Biofilm-Research Centre for Biointerfaces and Biomedical Science Department, Faculty of Health and Society, Malmo University, 20506, Malmo, Sweden"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Waldie", 
        "givenName": "Sarah", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Laue-Langevin", 
          "id": "https://www.grid.ac/institutes/grid.156520.5", 
          "name": [
            "Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moulin", 
        "givenName": "Martine", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Laue-Langevin", 
          "id": "https://www.grid.ac/institutes/grid.156520.5", 
          "name": [
            "Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Porcar", 
        "givenName": "Lionel", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graz University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.410413.3", 
          "name": [
            "Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria", 
            "Graz University of Technology, Institute of Molecular Biotechnology, NAWI Graz, BioTechMed Graz, Petersgasse 14, 8010, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pichler", 
        "givenName": "Harald", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graz University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.410413.3", 
          "name": [
            "Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria", 
            "Graz University of Technology, Institute of Organic Chemistry, NAWI Graz, Stremayrgasse 9, 8010, Graz, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Strohmeier", 
        "givenName": "Gernot A.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Rutherford Appleton Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.76978.37", 
          "name": [
            "Rutherford Appleton Laboratory, OX11 0QX, Harwell, Didcot, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Skoda", 
        "givenName": "Maximilian", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Keele University", 
          "id": "https://www.grid.ac/institutes/grid.9757.c", 
          "name": [
            "Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France", 
            "Life Sciences Department, Faculty of Natural Sciences, Keele University, ST5 5BG, Staffordshire, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Forsyth", 
        "givenName": "V. Trevor", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Laue-Langevin", 
          "id": "https://www.grid.ac/institutes/grid.156520.5", 
          "name": [
            "Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Haertlein", 
        "givenName": "Michael", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lund University", 
          "id": "https://www.grid.ac/institutes/grid.4514.4", 
          "name": [
            "Biofilm-Research Centre for Biointerfaces and Biomedical Science Department, Faculty of Health and Society, Malmo University, 20506, Malmo, Sweden", 
            "MAX IV Laboratory, Fotongatan 2, 225 92, Lund, Sweden"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Maric", 
        "givenName": "Selma", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Malm\u00f6 University", 
          "id": "https://www.grid.ac/institutes/grid.32995.34", 
          "name": [
            "Biofilm-Research Centre for Biointerfaces and Biomedical Science Department, Faculty of Health and Society, Malmo University, 20506, Malmo, Sweden"
          ], 
          "type": "Organization"
        }, 
        "familyName": "C\u00e1rdenas", 
        "givenName": "Marit\u00e9", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-3-642-54640-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003564516", 
          "https://doi.org/10.1007/978-3-642-54640-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-54640-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003564516", 
          "https://doi.org/10.1007/978-3-642-54640-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(02)75394-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004153623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamem.2011.07.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004720892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1529/biophysj.104.044438", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009929611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c5sm01490e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010784054"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c2sm00013j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015105633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889806005073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016142863"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(67)90003-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026381114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(67)90003-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026381114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/bs.mie.2015.11.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027129092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja507832e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027799413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-013-5156-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028653523", 
          "https://doi.org/10.1007/s00253-013-5156-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-014-6082-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030640777", 
          "https://doi.org/10.1007/s00253-014-6082-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00249-012-0826-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034275717", 
          "https://doi.org/10.1007/s00249-012-0826-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00249-016-1186-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034578617", 
          "https://doi.org/10.1007/s00249-016-1186-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00249-016-1186-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034578617", 
          "https://doi.org/10.1007/s00249-016-1186-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00249-008-0280-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034729241", 
          "https://doi.org/10.1007/s00249-008-0280-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00249-008-0280-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034729241", 
          "https://doi.org/10.1007/s00249-008-0280-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00249-010-0635-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035156253", 
          "https://doi.org/10.1007/s00249-010-0635-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamem.2015.06.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035457517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c6sm01777k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037394635"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.physb.2006.05.400", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038490895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/42408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040731787", 
          "https://doi.org/10.1038/42408"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fpls.2014.00072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040943293"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0049-3848(80)90218-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042031094"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi100891z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044815992"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi100891z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044815992"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c6sm00710d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046933357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1140/epjst/e2012-01680-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048561574", 
          "https://doi.org/10.1140/epjst/e2012-01680-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(03)74664-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049595617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00144a013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055162510"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0520840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0520840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201290"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi800123b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055208348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi800123b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055208348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi953057k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi953057k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055211502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/j100255a054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055661283"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la001382p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056142013"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la001382p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056142013"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la047389e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056147068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/la047389e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056147068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physreve.71.061924", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060733058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physreve.71.061924", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060733058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1116/1.4944830", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062178004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-07505-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091015371", 
          "https://doi.org/10.1038/s41598-017-07505-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41598-017-07505-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091015371", 
          "https://doi.org/10.1038/s41598-017-07505-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2017.10.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092535654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2017.10.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092535654"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.langmuir.7b02716", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1099646194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chemphyslip.2018.01.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100565640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2018.02.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100836812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2018.05.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105051018"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2018.09.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107157597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2018.09.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1107157597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.colsurfb.2019.01.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1111830057"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "The deuteration of biomolecules provides advanced opportunities for neutron scattering studies. For low resolution studies using techniques such as small-angle neutron scattering and neutron reflection, the level of deuteration of a sample can be varied to match the scattering length density of a specific D2O/H2O solvent mixture. This can be of major value in structural studies where specific regions of a complex system can be highlighted, and others rendered invisible. This is especially useful in analyses of the structure and dynamics of membrane components. In mammalian membranes, the presence of cholesterol is crucial in modulating the properties of lipids and in their interaction with proteins. Here, a protocol is described for the production of partially deuterated cholesterol which has a neutron scattering length density that matches that of 100% D2O solvent\u00a0(hereby named matchout cholesterol). The level of deuteration was determined by mass spectrometry and nuclear magnetic resonance. The cholesterol match-point was verified experimentally using small angle neutron scattering. The matchout cholesterol was used to investigate the incorporation of cholesterol in various phosphatidylcholine supported lipid bilayers by neutron reflectometry. The study included both saturated and unsaturated lipids, as well as lipids with varying chain lengths. It was found that cholesterol is distributed asymmetrically within the bilayer, positioned closer to the headgroups of the lipids than to the middle of the tail core, regardless of the phosphatidylcholine species.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-019-41439-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2774027", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions", 
    "pagination": "5118", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a045d60727dba233a19a4c8828c97a5da4fb306e1a4a75acabd4252103294fa2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30914734"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-019-41439-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112988342"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-019-41439-z", 
      "https://app.dimensions.ai/details/publication/pub.1112988342"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:02", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130830_00000006.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-019-41439-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41439-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41439-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41439-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41439-z'


 

This table displays all metadata directly associated to this object as RDF triples.

282 TRIPLES      21 PREDICATES      72 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-019-41439-z schema:about anzsrc-for:02
2 anzsrc-for:0299
3 schema:author N21553516556f4ff7a9296d95a9e8cc9c
4 schema:citation sg:pub.10.1007/978-3-642-54640-2
5 sg:pub.10.1007/s00249-008-0280-5
6 sg:pub.10.1007/s00249-010-0635-6
7 sg:pub.10.1007/s00249-012-0826-4
8 sg:pub.10.1007/s00249-016-1186-2
9 sg:pub.10.1007/s00253-013-5156-7
10 sg:pub.10.1007/s00253-014-6082-z
11 sg:pub.10.1038/42408
12 sg:pub.10.1038/s41598-017-07505-0
13 sg:pub.10.1140/epjst/e2012-01680-5
14 https://doi.org/10.1016/0005-2736(67)90003-x
15 https://doi.org/10.1016/0049-3848(80)90218-2
16 https://doi.org/10.1016/bs.mie.2015.11.001
17 https://doi.org/10.1016/j.bbamem.2011.07.022
18 https://doi.org/10.1016/j.bbamem.2015.06.006
19 https://doi.org/10.1016/j.bpj.2017.10.017
20 https://doi.org/10.1016/j.chemphyslip.2018.01.006
21 https://doi.org/10.1016/j.colsurfb.2018.02.009
22 https://doi.org/10.1016/j.colsurfb.2018.09.031
23 https://doi.org/10.1016/j.colsurfb.2019.01.040
24 https://doi.org/10.1016/j.physb.2006.05.400
25 https://doi.org/10.1016/j.str.2018.05.007
26 https://doi.org/10.1016/s0006-3495(02)75394-0
27 https://doi.org/10.1016/s0006-3495(03)74664-5
28 https://doi.org/10.1021/acs.langmuir.7b02716
29 https://doi.org/10.1021/bi00144a013
30 https://doi.org/10.1021/bi0520840
31 https://doi.org/10.1021/bi100891z
32 https://doi.org/10.1021/bi800123b
33 https://doi.org/10.1021/bi953057k
34 https://doi.org/10.1021/j100255a054
35 https://doi.org/10.1021/ja507832e
36 https://doi.org/10.1021/la001382p
37 https://doi.org/10.1021/la047389e
38 https://doi.org/10.1039/c2sm00013j
39 https://doi.org/10.1039/c5sm01490e
40 https://doi.org/10.1039/c6sm00710d
41 https://doi.org/10.1039/c6sm01777k
42 https://doi.org/10.1103/physreve.71.061924
43 https://doi.org/10.1107/s0021889806005073
44 https://doi.org/10.1116/1.4944830
45 https://doi.org/10.1529/biophysj.104.044438
46 https://doi.org/10.3389/fpls.2014.00072
47 schema:datePublished 2019-12
48 schema:datePublishedReg 2019-12-01
49 schema:description The deuteration of biomolecules provides advanced opportunities for neutron scattering studies. For low resolution studies using techniques such as small-angle neutron scattering and neutron reflection, the level of deuteration of a sample can be varied to match the scattering length density of a specific D<sub>2</sub>O/H<sub>2</sub>O solvent mixture. This can be of major value in structural studies where specific regions of a complex system can be highlighted, and others rendered invisible. This is especially useful in analyses of the structure and dynamics of membrane components. In mammalian membranes, the presence of cholesterol is crucial in modulating the properties of lipids and in their interaction with proteins. Here, a protocol is described for the production of partially deuterated cholesterol which has a neutron scattering length density that matches that of 100% D<sub>2</sub>O solvent (hereby named matchout cholesterol). The level of deuteration was determined by mass spectrometry and nuclear magnetic resonance. The cholesterol match-point was verified experimentally using small angle neutron scattering. The matchout cholesterol was used to investigate the incorporation of cholesterol in various phosphatidylcholine supported lipid bilayers by neutron reflectometry. The study included both saturated and unsaturated lipids, as well as lipids with varying chain lengths. It was found that cholesterol is distributed asymmetrically within the bilayer, positioned closer to the headgroups of the lipids than to the middle of the tail core, regardless of the phosphatidylcholine species.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree true
53 schema:isPartOf N9f015f75b97d430b890f541a2c6efa01
54 Nd3532e921514451a8469bb65ec45ac4e
55 sg:journal.1045337
56 schema:name The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions
57 schema:pagination 5118
58 schema:productId N3589e58d21e94bb797a430e09ef5e004
59 N538d77af1cd0402eb16e31d2d44963e9
60 Na98d21ea670640608b50d5bf701ac122
61 Nc40b5a9500d642a48102cdf87b6f616a
62 Nf96a982ff7204bd09c5d56d8c85919f8
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112988342
64 https://doi.org/10.1038/s41598-019-41439-z
65 schema:sdDatePublished 2019-04-11T14:02
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher N86187668f76c485daa2417c02929c56c
68 schema:url https://www.nature.com/articles/s41598-019-41439-z
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N21553516556f4ff7a9296d95a9e8cc9c rdf:first N955001b95af0497e821df0e30e09fb0a
73 rdf:rest Ne58b4829b0974810bc0c21ba463ecab8
74 N27740d8e205d4b678cda7f5790b19305 rdf:first N9d5909aead0b42308ed2060e61586b56
75 rdf:rest N2d052b2c500842bcb10763feb790ecc2
76 N2823adb8ea6c4871a5dfb081225f2771 schema:affiliation https://www.grid.ac/institutes/grid.156520.5
77 schema:familyName Moulin
78 schema:givenName Martine
79 rdf:type schema:Person
80 N299f845fb0ce4b23a0568efd539f8d6c schema:affiliation https://www.grid.ac/institutes/grid.4514.4
81 schema:familyName Maric
82 schema:givenName Selma
83 rdf:type schema:Person
84 N2d052b2c500842bcb10763feb790ecc2 rdf:first N881ff72bdd5242939e79e569b53ff503
85 rdf:rest N99b5686a2f1c41289fe6d558c84f12aa
86 N302b6acb12474cf4840fd471ca2a75c7 schema:affiliation https://www.grid.ac/institutes/grid.410413.3
87 schema:familyName Strohmeier
88 schema:givenName Gernot A.
89 rdf:type schema:Person
90 N3589e58d21e94bb797a430e09ef5e004 schema:name doi
91 schema:value 10.1038/s41598-019-41439-z
92 rdf:type schema:PropertyValue
93 N476038eea55c46288c5d9dd74951b945 rdf:first N299f845fb0ce4b23a0568efd539f8d6c
94 rdf:rest Nead6477eff814e76b98d00368f730768
95 N478b2fecf3f04d149a64ff74ae570c9f schema:affiliation https://www.grid.ac/institutes/grid.9757.c
96 schema:familyName Forsyth
97 schema:givenName V. Trevor
98 rdf:type schema:Person
99 N538d77af1cd0402eb16e31d2d44963e9 schema:name nlm_unique_id
100 schema:value 101563288
101 rdf:type schema:PropertyValue
102 N7b0209c79d1a44c1bacf526e0cacc1b5 schema:affiliation https://www.grid.ac/institutes/grid.76978.37
103 schema:familyName Skoda
104 schema:givenName Maximilian
105 rdf:type schema:Person
106 N86187668f76c485daa2417c02929c56c schema:name Springer Nature - SN SciGraph project
107 rdf:type schema:Organization
108 N881ff72bdd5242939e79e569b53ff503 schema:affiliation https://www.grid.ac/institutes/grid.410413.3
109 schema:familyName Pichler
110 schema:givenName Harald
111 rdf:type schema:Person
112 N955001b95af0497e821df0e30e09fb0a schema:affiliation https://www.grid.ac/institutes/grid.32995.34
113 schema:familyName Waldie
114 schema:givenName Sarah
115 rdf:type schema:Person
116 N99b5686a2f1c41289fe6d558c84f12aa rdf:first N302b6acb12474cf4840fd471ca2a75c7
117 rdf:rest Nb3c2ad2fe84b4d148b75ff596659daf1
118 N9d5909aead0b42308ed2060e61586b56 schema:affiliation https://www.grid.ac/institutes/grid.156520.5
119 schema:familyName Porcar
120 schema:givenName Lionel
121 rdf:type schema:Person
122 N9f015f75b97d430b890f541a2c6efa01 schema:issueNumber 1
123 rdf:type schema:PublicationIssue
124 Na98d21ea670640608b50d5bf701ac122 schema:name dimensions_id
125 schema:value pub.1112988342
126 rdf:type schema:PropertyValue
127 Nb3c2ad2fe84b4d148b75ff596659daf1 rdf:first N7b0209c79d1a44c1bacf526e0cacc1b5
128 rdf:rest Nd775021773874d84a31e779939b1a164
129 Nb88dc60dc7b64035ba770da4350b7d38 schema:affiliation https://www.grid.ac/institutes/grid.156520.5
130 schema:familyName Haertlein
131 schema:givenName Michael
132 rdf:type schema:Person
133 Nc40b5a9500d642a48102cdf87b6f616a schema:name readcube_id
134 schema:value a045d60727dba233a19a4c8828c97a5da4fb306e1a4a75acabd4252103294fa2
135 rdf:type schema:PropertyValue
136 Nd3532e921514451a8469bb65ec45ac4e schema:volumeNumber 9
137 rdf:type schema:PublicationVolume
138 Nd775021773874d84a31e779939b1a164 rdf:first N478b2fecf3f04d149a64ff74ae570c9f
139 rdf:rest Ne6ea78c7991a42cc8d5b5b768445413d
140 Ne524ed8f080e4151a80831220daff149 schema:affiliation https://www.grid.ac/institutes/grid.32995.34
141 schema:familyName Cárdenas
142 schema:givenName Marité
143 rdf:type schema:Person
144 Ne58b4829b0974810bc0c21ba463ecab8 rdf:first N2823adb8ea6c4871a5dfb081225f2771
145 rdf:rest N27740d8e205d4b678cda7f5790b19305
146 Ne6ea78c7991a42cc8d5b5b768445413d rdf:first Nb88dc60dc7b64035ba770da4350b7d38
147 rdf:rest N476038eea55c46288c5d9dd74951b945
148 Nead6477eff814e76b98d00368f730768 rdf:first Ne524ed8f080e4151a80831220daff149
149 rdf:rest rdf:nil
150 Nf96a982ff7204bd09c5d56d8c85919f8 schema:name pubmed_id
151 schema:value 30914734
152 rdf:type schema:PropertyValue
153 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
154 schema:name Physical Sciences
155 rdf:type schema:DefinedTerm
156 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
157 schema:name Other Physical Sciences
158 rdf:type schema:DefinedTerm
159 sg:grant.2774027 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41439-z
160 rdf:type schema:MonetaryGrant
161 sg:journal.1045337 schema:issn 2045-2322
162 schema:name Scientific Reports
163 rdf:type schema:Periodical
164 sg:pub.10.1007/978-3-642-54640-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003564516
165 https://doi.org/10.1007/978-3-642-54640-2
166 rdf:type schema:CreativeWork
167 sg:pub.10.1007/s00249-008-0280-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034729241
168 https://doi.org/10.1007/s00249-008-0280-5
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/s00249-010-0635-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035156253
171 https://doi.org/10.1007/s00249-010-0635-6
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/s00249-012-0826-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034275717
174 https://doi.org/10.1007/s00249-012-0826-4
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s00249-016-1186-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034578617
177 https://doi.org/10.1007/s00249-016-1186-2
178 rdf:type schema:CreativeWork
179 sg:pub.10.1007/s00253-013-5156-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028653523
180 https://doi.org/10.1007/s00253-013-5156-7
181 rdf:type schema:CreativeWork
182 sg:pub.10.1007/s00253-014-6082-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1030640777
183 https://doi.org/10.1007/s00253-014-6082-z
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/42408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040731787
186 https://doi.org/10.1038/42408
187 rdf:type schema:CreativeWork
188 sg:pub.10.1038/s41598-017-07505-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091015371
189 https://doi.org/10.1038/s41598-017-07505-0
190 rdf:type schema:CreativeWork
191 sg:pub.10.1140/epjst/e2012-01680-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048561574
192 https://doi.org/10.1140/epjst/e2012-01680-5
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/0005-2736(67)90003-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026381114
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/0049-3848(80)90218-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042031094
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/bs.mie.2015.11.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027129092
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.bbamem.2011.07.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004720892
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.bbamem.2015.06.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035457517
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/j.bpj.2017.10.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092535654
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.chemphyslip.2018.01.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100565640
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.colsurfb.2018.02.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100836812
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.colsurfb.2018.09.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1107157597
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/j.colsurfb.2019.01.040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111830057
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/j.physb.2006.05.400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038490895
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/j.str.2018.05.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105051018
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/s0006-3495(02)75394-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004153623
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/s0006-3495(03)74664-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049595617
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1021/acs.langmuir.7b02716 schema:sameAs https://app.dimensions.ai/details/publication/pub.1099646194
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1021/bi00144a013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055162510
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1021/bi0520840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055201290
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1021/bi100891z schema:sameAs https://app.dimensions.ai/details/publication/pub.1044815992
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1021/bi800123b schema:sameAs https://app.dimensions.ai/details/publication/pub.1055208348
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1021/bi953057k schema:sameAs https://app.dimensions.ai/details/publication/pub.1055211502
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1021/j100255a054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055661283
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1021/ja507832e schema:sameAs https://app.dimensions.ai/details/publication/pub.1027799413
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1021/la001382p schema:sameAs https://app.dimensions.ai/details/publication/pub.1056142013
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1021/la047389e schema:sameAs https://app.dimensions.ai/details/publication/pub.1056147068
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1039/c2sm00013j schema:sameAs https://app.dimensions.ai/details/publication/pub.1015105633
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1039/c5sm01490e schema:sameAs https://app.dimensions.ai/details/publication/pub.1010784054
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1039/c6sm00710d schema:sameAs https://app.dimensions.ai/details/publication/pub.1046933357
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1039/c6sm01777k schema:sameAs https://app.dimensions.ai/details/publication/pub.1037394635
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1103/physreve.71.061924 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060733058
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1107/s0021889806005073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016142863
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1116/1.4944830 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062178004
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1529/biophysj.104.044438 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009929611
257 rdf:type schema:CreativeWork
258 https://doi.org/10.3389/fpls.2014.00072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040943293
259 rdf:type schema:CreativeWork
260 https://www.grid.ac/institutes/grid.156520.5 schema:alternateName Institut Laue-Langevin
261 schema:name Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France
262 rdf:type schema:Organization
263 https://www.grid.ac/institutes/grid.32995.34 schema:alternateName Malmö University
264 schema:name Biofilm-Research Centre for Biointerfaces and Biomedical Science Department, Faculty of Health and Society, Malmo University, 20506, Malmo, Sweden
265 Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France
266 rdf:type schema:Organization
267 https://www.grid.ac/institutes/grid.410413.3 schema:alternateName Graz University of Technology
268 schema:name Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria
269 Graz University of Technology, Institute of Molecular Biotechnology, NAWI Graz, BioTechMed Graz, Petersgasse 14, 8010, Graz, Austria
270 Graz University of Technology, Institute of Organic Chemistry, NAWI Graz, Stremayrgasse 9, 8010, Graz, Austria
271 rdf:type schema:Organization
272 https://www.grid.ac/institutes/grid.4514.4 schema:alternateName Lund University
273 schema:name Biofilm-Research Centre for Biointerfaces and Biomedical Science Department, Faculty of Health and Society, Malmo University, 20506, Malmo, Sweden
274 MAX IV Laboratory, Fotongatan 2, 225 92, Lund, Sweden
275 rdf:type schema:Organization
276 https://www.grid.ac/institutes/grid.76978.37 schema:alternateName Rutherford Appleton Laboratory
277 schema:name Rutherford Appleton Laboratory, OX11 0QX, Harwell, Didcot, UK
278 rdf:type schema:Organization
279 https://www.grid.ac/institutes/grid.9757.c schema:alternateName Keele University
280 schema:name Institut Laue-Langevin, 71 Avenue des Martyrs, 38042, Grenoble, Cedex 9, France
281 Life Sciences Department, Faculty of Natural Sciences, Keele University, ST5 5BG, Staffordshire, UK
282 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...