Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Takahiro Nakatani, Norihisa Yasui, Issei Tamura, Atsuko Yamashita

ABSTRACT

Green fluorescent protein (GFP) is amenable to recombinant expression in various kinds of cells and is widely used in life science research. We found that the recombinant expression of GFPuv, a commonly-used mutant of GFP, in E. coli produced two distinct molecular species as judged by in-gel fluorescence SDS-PAGE. These molecular species, namely form I and II, could be separately purified by anion-exchange chromatography without any remarkable differences in the fluorescence spectra. Mass spectrometric analyses revealed that the molecular mass of form I is almost the same as the calculated value, while that of form II is approximately 1 Da larger than that of form I. Further mass spectrometric top-down sequencing pinpointed the modification in GFPuv form II, where the ε-amino group of the C-terminal Lys238 residue is converted into the hydroxyl group. No equivalent modification was observed in the native GFP in jellyfish Aequorea victoria, suggesting that this modification is not physiologically relevant. Crystal structure analysis of the two species verified the structural identity of the backbone and the vicinity of the chromophore. The modification found in this study may also be generated in other GFP variants as well as in other recombinant expression systems. More... »

PAGES

4722

Journal

TITLE

Scientific Reports

ISSUE

1

VOLUME

9

Author Affiliations

From Grant

  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-41309-8

    DOI

    http://dx.doi.org/10.1038/s41598-019-41309-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112855866

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30886277


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Okayama University", 
              "id": "https://www.grid.ac/institutes/grid.261356.5", 
              "name": [
                "Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-naka, Kita-ku, 700-8530, Okayama, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nakatani", 
            "givenName": "Takahiro", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Okayama University", 
              "id": "https://www.grid.ac/institutes/grid.261356.5", 
              "name": [
                "Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-naka, Kita-ku, 700-8530, Okayama, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yasui", 
            "givenName": "Norihisa", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Okayama University", 
              "id": "https://www.grid.ac/institutes/grid.261356.5", 
              "name": [
                "Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-naka, Kita-ku, 700-8530, Okayama, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tamura", 
            "givenName": "Issei", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Okayama University", 
              "id": "https://www.grid.ac/institutes/grid.261356.5", 
              "name": [
                "Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-naka, Kita-ku, 700-8530, Okayama, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yamashita", 
            "givenName": "Atsuko", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1107/s0907444910007493", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444910007493", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/mcp.m700076-mcp200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000996052"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1068539", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002422087"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ab.2011.01.038", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004540925"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(97)76066-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004907170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444912001308", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005539810"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/1097-0134(20001201)41:4<429::aid-prot10>3.0.co;2-d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006478292"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0396-315", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007123993", 
              "https://doi.org/10.1038/nbt0396-315"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.str.2012.06.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009108816"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1044", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009347902", 
              "https://doi.org/10.1038/nbt1044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1044", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009347902", 
              "https://doi.org/10.1038/nbt1044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2008.44", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011300232", 
              "https://doi.org/10.1038/nprot.2008.44"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2005.08.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014794069"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2005.08.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014794069"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/369400a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016931928", 
              "https://doi.org/10.1038/369400a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444909042073", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018458004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444909042073", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018458004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.98.1.69", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018690200"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.26.12501", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020397958"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444996012255", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020405374"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0014-5793(99)01044-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023277959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.94.6.2306", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023824800"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pmic.200300744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025026586"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0968-0004(00)89099-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030803272"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.052018199", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032618341"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jcp.1030590302", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037056216"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.str.2006.01.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040537865"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth0406-303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047687178", 
              "https://doi.org/10.1038/nmeth0406-303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth0406-303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047687178", 
              "https://doi.org/10.1038/nmeth0406-303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0704546104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048108080"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.biochem.67.1.509", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050836823"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444909042589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051254013"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1107/s0907444909042589", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051254013"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/pro.707", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053052609"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00838a015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055190285"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi970281w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055213618"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi970281w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055213618"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.8303295", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062654568"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.redox.2017.03.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084105512"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.redox.2017.03.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084105512"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0181186", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090577589"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-12", 
        "datePublishedReg": "2019-12-01", 
        "description": "Green fluorescent protein (GFP) is amenable to recombinant expression in various kinds of cells and is widely used in life science research. We found that the recombinant expression of GFPuv, a commonly-used mutant of GFP, in E. coli produced two distinct molecular species as judged by in-gel fluorescence SDS-PAGE. These molecular species, namely form I and II, could be separately purified by anion-exchange chromatography without any remarkable differences in the fluorescence spectra. Mass spectrometric analyses revealed that the molecular mass of form I is almost the same as the calculated value, while that of form II is approximately 1\u2009Da larger than that of form I. Further mass spectrometric top-down sequencing pinpointed the modification in GFPuv form II, where the \u03b5-amino group of the C-terminal Lys238 residue is converted into the hydroxyl group. No equivalent modification was observed in the native GFP in jellyfish Aequorea victoria, suggesting that this modification is not physiologically relevant. Crystal structure analysis of the two species verified the structural identity of the backbone and the vicinity of the chromophore. The modification found in this study may also be generated in other GFP variants as well as in other recombinant expression systems.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/s41598-019-41309-8", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.5864476", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6821638", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6131049", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "name": "Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli", 
        "pagination": "4722", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "a8e28bf3f64675fbde30c0a004a231904cb4fc87c132844291af085092d49650"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30886277"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101563288"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-019-41309-8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1112855866"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-019-41309-8", 
          "https://app.dimensions.ai/details/publication/pub.1112855866"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:19", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78950_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/s41598-019-41309-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41309-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41309-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41309-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41309-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    198 TRIPLES      21 PREDICATES      63 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-019-41309-8 schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author N9e078565e5be4e4082e2d88cfc47898a
    4 schema:citation sg:pub.10.1038/369400a0
    5 sg:pub.10.1038/nbt0396-315
    6 sg:pub.10.1038/nbt1044
    7 sg:pub.10.1038/nmeth0406-303
    8 sg:pub.10.1038/nprot.2008.44
    9 https://doi.org/10.1002/1097-0134(20001201)41:4<429::aid-prot10>3.0.co;2-d
    10 https://doi.org/10.1002/jcp.1030590302
    11 https://doi.org/10.1002/pmic.200300744
    12 https://doi.org/10.1002/pro.707
    13 https://doi.org/10.1016/j.ab.2011.01.038
    14 https://doi.org/10.1016/j.jbiotec.2005.08.006
    15 https://doi.org/10.1016/j.redox.2017.03.002
    16 https://doi.org/10.1016/j.str.2006.01.013
    17 https://doi.org/10.1016/j.str.2012.06.009
    18 https://doi.org/10.1016/s0014-5793(99)01044-3
    19 https://doi.org/10.1016/s0076-6879(97)76066-x
    20 https://doi.org/10.1016/s0968-0004(00)89099-4
    21 https://doi.org/10.1021/bi00838a015
    22 https://doi.org/10.1021/bi970281w
    23 https://doi.org/10.1073/pnas.052018199
    24 https://doi.org/10.1073/pnas.0704546104
    25 https://doi.org/10.1073/pnas.91.26.12501
    26 https://doi.org/10.1073/pnas.94.6.2306
    27 https://doi.org/10.1073/pnas.98.1.69
    28 https://doi.org/10.1074/mcp.m700076-mcp200
    29 https://doi.org/10.1107/s0907444909042073
    30 https://doi.org/10.1107/s0907444909042589
    31 https://doi.org/10.1107/s0907444910007493
    32 https://doi.org/10.1107/s0907444912001308
    33 https://doi.org/10.1107/s0907444996012255
    34 https://doi.org/10.1126/science.1068539
    35 https://doi.org/10.1126/science.8303295
    36 https://doi.org/10.1146/annurev.biochem.67.1.509
    37 https://doi.org/10.1371/journal.pone.0181186
    38 schema:datePublished 2019-12
    39 schema:datePublishedReg 2019-12-01
    40 schema:description Green fluorescent protein (GFP) is amenable to recombinant expression in various kinds of cells and is widely used in life science research. We found that the recombinant expression of GFPuv, a commonly-used mutant of GFP, in E. coli produced two distinct molecular species as judged by in-gel fluorescence SDS-PAGE. These molecular species, namely form I and II, could be separately purified by anion-exchange chromatography without any remarkable differences in the fluorescence spectra. Mass spectrometric analyses revealed that the molecular mass of form I is almost the same as the calculated value, while that of form II is approximately 1 Da larger than that of form I. Further mass spectrometric top-down sequencing pinpointed the modification in GFPuv form II, where the ε-amino group of the C-terminal Lys238 residue is converted into the hydroxyl group. No equivalent modification was observed in the native GFP in jellyfish Aequorea victoria, suggesting that this modification is not physiologically relevant. Crystal structure analysis of the two species verified the structural identity of the backbone and the vicinity of the chromophore. The modification found in this study may also be generated in other GFP variants as well as in other recombinant expression systems.
    41 schema:genre research_article
    42 schema:inLanguage en
    43 schema:isAccessibleForFree true
    44 schema:isPartOf N8348a419e0cf4c429aee717e7ae2bb79
    45 Nb05c24158d114420b55e6c45846afc2a
    46 sg:journal.1045337
    47 schema:name Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli
    48 schema:pagination 4722
    49 schema:productId N0b01661e6c2f46c5b00e752068540c4b
    50 N5134112992034980897f94a6aa2547ae
    51 N5897603237f746ccb216c916c594f9df
    52 N75c8859acd8c42799da91057848904cc
    53 Ne4916a99e0204bc49ecec880204d8dd3
    54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112855866
    55 https://doi.org/10.1038/s41598-019-41309-8
    56 schema:sdDatePublished 2019-04-11T13:19
    57 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    58 schema:sdPublisher Ne61a0b45badb42a4adcace0b57bb918b
    59 schema:url https://www.nature.com/articles/s41598-019-41309-8
    60 sgo:license sg:explorer/license/
    61 sgo:sdDataset articles
    62 rdf:type schema:ScholarlyArticle
    63 N0b01661e6c2f46c5b00e752068540c4b schema:name dimensions_id
    64 schema:value pub.1112855866
    65 rdf:type schema:PropertyValue
    66 N507169d5fa0c44f786f6e1d702c414bf schema:affiliation https://www.grid.ac/institutes/grid.261356.5
    67 schema:familyName Tamura
    68 schema:givenName Issei
    69 rdf:type schema:Person
    70 N5134112992034980897f94a6aa2547ae schema:name nlm_unique_id
    71 schema:value 101563288
    72 rdf:type schema:PropertyValue
    73 N5897603237f746ccb216c916c594f9df schema:name pubmed_id
    74 schema:value 30886277
    75 rdf:type schema:PropertyValue
    76 N5d8776def7ed408d989a1d7c61ede755 schema:affiliation https://www.grid.ac/institutes/grid.261356.5
    77 schema:familyName Nakatani
    78 schema:givenName Takahiro
    79 rdf:type schema:Person
    80 N6a779fdf640645f59a071d48b1d3babb rdf:first N7c7cb4ff99714bf29e2e5d338aaa3d1a
    81 rdf:rest rdf:nil
    82 N75c8859acd8c42799da91057848904cc schema:name doi
    83 schema:value 10.1038/s41598-019-41309-8
    84 rdf:type schema:PropertyValue
    85 N7c7cb4ff99714bf29e2e5d338aaa3d1a schema:affiliation https://www.grid.ac/institutes/grid.261356.5
    86 schema:familyName Yamashita
    87 schema:givenName Atsuko
    88 rdf:type schema:Person
    89 N8348a419e0cf4c429aee717e7ae2bb79 schema:issueNumber 1
    90 rdf:type schema:PublicationIssue
    91 N8aaefbf892204e33bef13fc457aad42d rdf:first N507169d5fa0c44f786f6e1d702c414bf
    92 rdf:rest N6a779fdf640645f59a071d48b1d3babb
    93 N9cd89edb6d344575b92cfe75f43dad7e schema:affiliation https://www.grid.ac/institutes/grid.261356.5
    94 schema:familyName Yasui
    95 schema:givenName Norihisa
    96 rdf:type schema:Person
    97 N9e078565e5be4e4082e2d88cfc47898a rdf:first N5d8776def7ed408d989a1d7c61ede755
    98 rdf:rest Nc6d03a4a479844a99db2f08c687f8bbe
    99 Nb05c24158d114420b55e6c45846afc2a schema:volumeNumber 9
    100 rdf:type schema:PublicationVolume
    101 Nc6d03a4a479844a99db2f08c687f8bbe rdf:first N9cd89edb6d344575b92cfe75f43dad7e
    102 rdf:rest N8aaefbf892204e33bef13fc457aad42d
    103 Ne4916a99e0204bc49ecec880204d8dd3 schema:name readcube_id
    104 schema:value a8e28bf3f64675fbde30c0a004a231904cb4fc87c132844291af085092d49650
    105 rdf:type schema:PropertyValue
    106 Ne61a0b45badb42a4adcace0b57bb918b schema:name Springer Nature - SN SciGraph project
    107 rdf:type schema:Organization
    108 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    109 schema:name Biological Sciences
    110 rdf:type schema:DefinedTerm
    111 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    112 schema:name Biochemistry and Cell Biology
    113 rdf:type schema:DefinedTerm
    114 sg:grant.5864476 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41309-8
    115 rdf:type schema:MonetaryGrant
    116 sg:grant.6131049 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41309-8
    117 rdf:type schema:MonetaryGrant
    118 sg:grant.6821638 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41309-8
    119 rdf:type schema:MonetaryGrant
    120 sg:journal.1045337 schema:issn 2045-2322
    121 schema:name Scientific Reports
    122 rdf:type schema:Periodical
    123 sg:pub.10.1038/369400a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016931928
    124 https://doi.org/10.1038/369400a0
    125 rdf:type schema:CreativeWork
    126 sg:pub.10.1038/nbt0396-315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007123993
    127 https://doi.org/10.1038/nbt0396-315
    128 rdf:type schema:CreativeWork
    129 sg:pub.10.1038/nbt1044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009347902
    130 https://doi.org/10.1038/nbt1044
    131 rdf:type schema:CreativeWork
    132 sg:pub.10.1038/nmeth0406-303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047687178
    133 https://doi.org/10.1038/nmeth0406-303
    134 rdf:type schema:CreativeWork
    135 sg:pub.10.1038/nprot.2008.44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011300232
    136 https://doi.org/10.1038/nprot.2008.44
    137 rdf:type schema:CreativeWork
    138 https://doi.org/10.1002/1097-0134(20001201)41:4<429::aid-prot10>3.0.co;2-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1006478292
    139 rdf:type schema:CreativeWork
    140 https://doi.org/10.1002/jcp.1030590302 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037056216
    141 rdf:type schema:CreativeWork
    142 https://doi.org/10.1002/pmic.200300744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025026586
    143 rdf:type schema:CreativeWork
    144 https://doi.org/10.1002/pro.707 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053052609
    145 rdf:type schema:CreativeWork
    146 https://doi.org/10.1016/j.ab.2011.01.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004540925
    147 rdf:type schema:CreativeWork
    148 https://doi.org/10.1016/j.jbiotec.2005.08.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014794069
    149 rdf:type schema:CreativeWork
    150 https://doi.org/10.1016/j.redox.2017.03.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084105512
    151 rdf:type schema:CreativeWork
    152 https://doi.org/10.1016/j.str.2006.01.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040537865
    153 rdf:type schema:CreativeWork
    154 https://doi.org/10.1016/j.str.2012.06.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009108816
    155 rdf:type schema:CreativeWork
    156 https://doi.org/10.1016/s0014-5793(99)01044-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023277959
    157 rdf:type schema:CreativeWork
    158 https://doi.org/10.1016/s0076-6879(97)76066-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004907170
    159 rdf:type schema:CreativeWork
    160 https://doi.org/10.1016/s0968-0004(00)89099-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030803272
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1021/bi00838a015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055190285
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1021/bi970281w schema:sameAs https://app.dimensions.ai/details/publication/pub.1055213618
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1073/pnas.052018199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032618341
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1073/pnas.0704546104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048108080
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1073/pnas.91.26.12501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020397958
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1073/pnas.94.6.2306 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023824800
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1073/pnas.98.1.69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018690200
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1074/mcp.m700076-mcp200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000996052
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1107/s0907444909042073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018458004
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1107/s0907444909042589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051254013
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1107/s0907444910007493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235100
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1107/s0907444912001308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005539810
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1107/s0907444996012255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020405374
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1126/science.1068539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002422087
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1126/science.8303295 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062654568
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1146/annurev.biochem.67.1.509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050836823
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1371/journal.pone.0181186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090577589
    195 rdf:type schema:CreativeWork
    196 https://www.grid.ac/institutes/grid.261356.5 schema:alternateName Okayama University
    197 schema:name Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1, Tsushima-naka, Kita-ku, 700-8530, Okayama, Japan
    198 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...