Evolutionary diversification of the RomR protein of the invasive deltaproteobacterium, Bdellovibrio bacteriovorus View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Rebecca C. Lowry, David S. Milner, Asmaa M. S. Al-Bayati, Carey Lambert, Vanessa I. Francis, Steven L. Porter, R. E. Sockett

ABSTRACT

Bdellovibrio bacteriovorus is a predatory deltaproteobacterium that encounters individual Gram-negative prey bacteria with gliding or swimming motility, and then is able to invade such prey cells via type IVa pilus-dependent mechanisms. Movement control (pili or gliding) in other deltaproteobacteria, such as the pack hunting Myxococcus xanthus, uses a response regulator protein, RomRMx (which dynamically relocalises between the cell poles) and a GTPase, MglAMx, previously postulated as an interface between the FrzMx chemosensory system and gliding or pilus-motility apparatus, to produce regulated bidirectional motility. In contrast, B. bacteriovorus predation is a more singular encounter between a lone predator and prey; contact is always via the piliated, non-flagellar pole of the predator, involving MglABd, but no Frz system. In this new study, tracking fluorescent RomRBd microscopically during predatory growth shows that it does not dynamically relocalise, in contrast to the M. xanthus protein; instead having possible roles in growth events. Furthermore, transcriptional start analysis, site-directed mutagenesis and bacterial two-hybrid interaction studies, indicate an evolutionary loss of RomRBd activation (via receiver domain phosphorylation) in this lone hunting bacterium, demonstrating divergence from its bipolar role in motility in pack-hunting M. xanthus and further evolution that may differentiate lone from pack predators. More... »

PAGES

5007

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-41263-5

DOI

http://dx.doi.org/10.1038/s41598-019-41263-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112895644

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30899045


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Nottingham", 
          "id": "https://www.grid.ac/institutes/grid.4563.4", 
          "name": [
            "School of Life Sciences, University of Nottingham, Medical School, Nottingham, United Kingdom"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lowry", 
        "givenName": "Rebecca C.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Exeter", 
          "id": "https://www.grid.ac/institutes/grid.8391.3", 
          "name": [
            "School of Life Sciences, University of Nottingham, Medical School, Nottingham, United Kingdom", 
            "Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, United Kingdom"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Milner", 
        "givenName": "David S.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Nottingham", 
          "id": "https://www.grid.ac/institutes/grid.4563.4", 
          "name": [
            "School of Life Sciences, University of Nottingham, Medical School, Nottingham, United Kingdom", 
            "Northern Technical University, Mosul, Iraq"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Al-Bayati", 
        "givenName": "Asmaa M. S.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Nottingham", 
          "id": "https://www.grid.ac/institutes/grid.4563.4", 
          "name": [
            "School of Life Sciences, University of Nottingham, Medical School, Nottingham, United Kingdom"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lambert", 
        "givenName": "Carey", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Exeter", 
          "id": "https://www.grid.ac/institutes/grid.8391.3", 
          "name": [
            "Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, United Kingdom"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Francis", 
        "givenName": "Vanessa I.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Exeter", 
          "id": "https://www.grid.ac/institutes/grid.8391.3", 
          "name": [
            "Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, United Kingdom"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Porter", 
        "givenName": "Steven L.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Nottingham", 
          "id": "https://www.grid.ac/institutes/grid.4563.4", 
          "name": [
            "School of Life Sciences, University of Nottingham, Medical School, Nottingham, United Kingdom"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sockett", 
        "givenName": "R. E.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.0609567103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000562234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2008.02.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003339222"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(02)01031-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004417516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(02)01031-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004417516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2010.114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005616601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2010.114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005616601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1002951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006605306"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.02095-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008403597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0079759", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008597050"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.1000306", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010887892"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/9780471729259.mc07b02s9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016659008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0015628", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016770500"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2006.05081.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017001124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2006.05081.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017001124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1004253", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018384224"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.micro.091208.073214", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019793505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2008.06238.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019917502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.01942-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020614923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.10.5752", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025735342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-micro-092611-150039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026512129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2008.10.027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028195096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2008.06426.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028925250"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m408855200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030331288"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00224-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032623020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mib.2012.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035716056"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/1758-2229.12297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036658810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(10)71001-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037340173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.1000430", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037672662"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-13-670", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041430742", 
          "https://doi.org/10.1186/1471-2164-13-670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.69.1.183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042694911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.micro.091208.073346", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042950995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1001220", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044040081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00914-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044438575"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1093027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046192024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1002493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046858275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/msb4100203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049071602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/msb4100203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049071602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmicrobiol.2016.77", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049117060", 
          "https://doi.org/10.1038/nmicrobiol.2016.77"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmicrobiol.2016.77", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049117060", 
          "https://doi.org/10.1038/nmicrobiol.2016.77"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601877", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049206759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00640-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049436764"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1002872", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052379954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2008.04.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052593185"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/mic.0.000092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060393999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/mic.0.036137-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060394781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/mic.0.036137-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060394781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41564-018-0203-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105584555", 
          "https://doi.org/10.1038/s41564-018-0203-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41564-018-0203-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105584555", 
          "https://doi.org/10.1038/s41564-018-0203-x"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Bdellovibrio bacteriovorus is a predatory deltaproteobacterium that encounters individual Gram-negative prey bacteria with gliding or swimming motility, and then is able to invade such prey cells via type IVa pilus-dependent mechanisms. Movement control (pili or gliding) in other deltaproteobacteria, such as the pack hunting Myxococcus xanthus, uses a response regulator protein, RomRMx (which dynamically relocalises between the cell poles) and a GTPase, MglAMx, previously postulated as an interface between the FrzMx chemosensory system and gliding or pilus-motility apparatus, to produce regulated bidirectional motility. In contrast, B. bacteriovorus predation is a more singular encounter between a lone predator and prey; contact is always via the piliated, non-flagellar pole of the predator, involving MglABd, but no Frz system. In this new study, tracking fluorescent RomRBd microscopically during predatory growth shows that it does not dynamically relocalise, in contrast to the M. xanthus protein; instead having possible roles in growth events. Furthermore, transcriptional start analysis, site-directed mutagenesis and bacterial two-hybrid interaction studies, indicate an evolutionary loss of RomRBd activation (via receiver domain phosphorylation) in this lone hunting bacterium, demonstrating divergence from its bipolar role in motility in pack-hunting M. xanthus and further evolution that may differentiate lone from pack predators.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-019-41263-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4850315", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3496621", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.3956893", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Evolutionary diversification of the RomR protein of the invasive deltaproteobacterium, Bdellovibrio bacteriovorus", 
    "pagination": "5007", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0ae0f4513760f63ac6db4b3dbdfbc3e49b994cfb04da5f0b9c750a6e65eadf73"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30899045"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-019-41263-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112895644"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-019-41263-5", 
      "https://app.dimensions.ai/details/publication/pub.1112895644"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78947_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-019-41263-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41263-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41263-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41263-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-41263-5'


 

This table displays all metadata directly associated to this object as RDF triples.

240 TRIPLES      21 PREDICATES      70 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-019-41263-5 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Ne4aa84952f2242fd9d57ad04ccee8434
4 schema:citation sg:pub.10.1038/nmicrobiol.2016.77
5 sg:pub.10.1038/s41564-018-0203-x
6 sg:pub.10.1186/1471-2164-13-670
7 https://doi.org/10.1002/9780471729259.mc07b02s9
8 https://doi.org/10.1016/j.cell.2008.04.040
9 https://doi.org/10.1016/j.jmb.2008.10.027
10 https://doi.org/10.1016/j.mib.2012.10.005
11 https://doi.org/10.1016/j.tim.2008.02.006
12 https://doi.org/10.1016/s0022-2836(02)01031-8
13 https://doi.org/10.1016/s0076-6879(10)71001-6
14 https://doi.org/10.1038/emboj.2010.114
15 https://doi.org/10.1038/msb4100203
16 https://doi.org/10.1038/sj.emboj.7601877
17 https://doi.org/10.1073/pnas.0609567103
18 https://doi.org/10.1073/pnas.95.10.5752
19 https://doi.org/10.1074/jbc.m408855200
20 https://doi.org/10.1099/mic.0.000092
21 https://doi.org/10.1099/mic.0.036137-0
22 https://doi.org/10.1111/1758-2229.12297
23 https://doi.org/10.1111/j.1365-2958.2006.05081.x
24 https://doi.org/10.1111/j.1365-2958.2008.06238.x
25 https://doi.org/10.1111/j.1365-2958.2008.06426.x
26 https://doi.org/10.1126/science.1093027
27 https://doi.org/10.1128/aem.00640-07
28 https://doi.org/10.1128/jb.00224-11
29 https://doi.org/10.1128/jb.00914-10
30 https://doi.org/10.1128/jb.01942-06
31 https://doi.org/10.1128/jb.02095-14
32 https://doi.org/10.1146/annurev-micro-092611-150039
33 https://doi.org/10.1146/annurev.biochem.69.1.183
34 https://doi.org/10.1146/annurev.micro.091208.073214
35 https://doi.org/10.1146/annurev.micro.091208.073346
36 https://doi.org/10.1371/journal.pbio.1000306
37 https://doi.org/10.1371/journal.pbio.1000430
38 https://doi.org/10.1371/journal.pgen.1001220
39 https://doi.org/10.1371/journal.pgen.1002872
40 https://doi.org/10.1371/journal.pgen.1002951
41 https://doi.org/10.1371/journal.pgen.1004253
42 https://doi.org/10.1371/journal.pone.0015628
43 https://doi.org/10.1371/journal.pone.0079759
44 https://doi.org/10.1371/journal.ppat.1002493
45 schema:datePublished 2019-12
46 schema:datePublishedReg 2019-12-01
47 schema:description Bdellovibrio bacteriovorus is a predatory deltaproteobacterium that encounters individual Gram-negative prey bacteria with gliding or swimming motility, and then is able to invade such prey cells via type IVa pilus-dependent mechanisms. Movement control (pili or gliding) in other deltaproteobacteria, such as the pack hunting Myxococcus xanthus, uses a response regulator protein, RomR<sub>Mx</sub> (which dynamically relocalises between the cell poles) and a GTPase, MglA<sub>Mx</sub>, previously postulated as an interface between the Frz<sub>Mx</sub> chemosensory system and gliding or pilus-motility apparatus, to produce regulated bidirectional motility. In contrast, B. bacteriovorus predation is a more singular encounter between a lone predator and prey; contact is always via the piliated, non-flagellar pole of the predator, involving MglA<sub>Bd</sub>, but no Frz system. In this new study, tracking fluorescent RomR<sub>Bd</sub> microscopically during predatory growth shows that it does not dynamically relocalise, in contrast to the M. xanthus protein; instead having possible roles in growth events. Furthermore, transcriptional start analysis, site-directed mutagenesis and bacterial two-hybrid interaction studies, indicate an evolutionary loss of RomR<sub>Bd</sub> activation (via receiver domain phosphorylation) in this lone hunting bacterium, demonstrating divergence from its bipolar role in motility in pack-hunting M. xanthus and further evolution that may differentiate lone from pack predators.
48 schema:genre research_article
49 schema:inLanguage en
50 schema:isAccessibleForFree true
51 schema:isPartOf Nd372d9fd3e5c44dc80a02da23223c0eb
52 Nd8c59685465645d2bbf675b28ab07ac9
53 sg:journal.1045337
54 schema:name Evolutionary diversification of the RomR protein of the invasive deltaproteobacterium, Bdellovibrio bacteriovorus
55 schema:pagination 5007
56 schema:productId N362495f1f3084c9c8d3daedf33c81018
57 N477acd9513734e5b8d9b64d7f875fa49
58 N4a9d296a260e411ca5fd97b11cdf7a0d
59 Na0738393076a48f980464e18b4c1615e
60 Nc4acbfada00b4f5da2196e7261530609
61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112895644
62 https://doi.org/10.1038/s41598-019-41263-5
63 schema:sdDatePublished 2019-04-11T13:18
64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
65 schema:sdPublisher N8cd300618a5e46de96368671917f18c3
66 schema:url https://www.nature.com/articles/s41598-019-41263-5
67 sgo:license sg:explorer/license/
68 sgo:sdDataset articles
69 rdf:type schema:ScholarlyArticle
70 N0453bba009c6415fba9630e4ee66b8e7 schema:affiliation https://www.grid.ac/institutes/grid.8391.3
71 schema:familyName Milner
72 schema:givenName David S.
73 rdf:type schema:Person
74 N0b3a29a6f9dc4d7b9bb64b58c267ace2 rdf:first N89a97453e9944b70934913902100c909
75 rdf:rest rdf:nil
76 N2f2216abca694963b98f377f645fba03 schema:affiliation https://www.grid.ac/institutes/grid.4563.4
77 schema:familyName Lowry
78 schema:givenName Rebecca C.
79 rdf:type schema:Person
80 N362495f1f3084c9c8d3daedf33c81018 schema:name readcube_id
81 schema:value 0ae0f4513760f63ac6db4b3dbdfbc3e49b994cfb04da5f0b9c750a6e65eadf73
82 rdf:type schema:PropertyValue
83 N3feb928943a2438d826b21464383e5ed rdf:first N622999024a794912ba7957835737516a
84 rdf:rest N4faf673309c34860ab6fbd5f2f0d8b3a
85 N477acd9513734e5b8d9b64d7f875fa49 schema:name pubmed_id
86 schema:value 30899045
87 rdf:type schema:PropertyValue
88 N4901eaa51a95498e915b6e8e0550f4c6 schema:affiliation https://www.grid.ac/institutes/grid.4563.4
89 schema:familyName Al-Bayati
90 schema:givenName Asmaa M. S.
91 rdf:type schema:Person
92 N4a9d296a260e411ca5fd97b11cdf7a0d schema:name dimensions_id
93 schema:value pub.1112895644
94 rdf:type schema:PropertyValue
95 N4faf673309c34860ab6fbd5f2f0d8b3a rdf:first N7a3f7df092944ac285026c2df16799be
96 rdf:rest N5b57af8ea2864c04a21a6ae5f834b79e
97 N5b57af8ea2864c04a21a6ae5f834b79e rdf:first Nbe33cd29f0d448098c895cdd5fdb3ae6
98 rdf:rest N0b3a29a6f9dc4d7b9bb64b58c267ace2
99 N622999024a794912ba7957835737516a schema:affiliation https://www.grid.ac/institutes/grid.4563.4
100 schema:familyName Lambert
101 schema:givenName Carey
102 rdf:type schema:Person
103 N6be53d4f1ae04f4db12889f35153dcc7 rdf:first N0453bba009c6415fba9630e4ee66b8e7
104 rdf:rest Nd5c2bdde5f1a4c5084f8833cf3a167c4
105 N7a3f7df092944ac285026c2df16799be schema:affiliation https://www.grid.ac/institutes/grid.8391.3
106 schema:familyName Francis
107 schema:givenName Vanessa I.
108 rdf:type schema:Person
109 N89a97453e9944b70934913902100c909 schema:affiliation https://www.grid.ac/institutes/grid.4563.4
110 schema:familyName Sockett
111 schema:givenName R. E.
112 rdf:type schema:Person
113 N8cd300618a5e46de96368671917f18c3 schema:name Springer Nature - SN SciGraph project
114 rdf:type schema:Organization
115 Na0738393076a48f980464e18b4c1615e schema:name nlm_unique_id
116 schema:value 101563288
117 rdf:type schema:PropertyValue
118 Nbe33cd29f0d448098c895cdd5fdb3ae6 schema:affiliation https://www.grid.ac/institutes/grid.8391.3
119 schema:familyName Porter
120 schema:givenName Steven L.
121 rdf:type schema:Person
122 Nc4acbfada00b4f5da2196e7261530609 schema:name doi
123 schema:value 10.1038/s41598-019-41263-5
124 rdf:type schema:PropertyValue
125 Nd372d9fd3e5c44dc80a02da23223c0eb schema:issueNumber 1
126 rdf:type schema:PublicationIssue
127 Nd5c2bdde5f1a4c5084f8833cf3a167c4 rdf:first N4901eaa51a95498e915b6e8e0550f4c6
128 rdf:rest N3feb928943a2438d826b21464383e5ed
129 Nd8c59685465645d2bbf675b28ab07ac9 schema:volumeNumber 9
130 rdf:type schema:PublicationVolume
131 Ne4aa84952f2242fd9d57ad04ccee8434 rdf:first N2f2216abca694963b98f377f645fba03
132 rdf:rest N6be53d4f1ae04f4db12889f35153dcc7
133 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
134 schema:name Biological Sciences
135 rdf:type schema:DefinedTerm
136 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
137 schema:name Biochemistry and Cell Biology
138 rdf:type schema:DefinedTerm
139 sg:grant.3496621 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41263-5
140 rdf:type schema:MonetaryGrant
141 sg:grant.3956893 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41263-5
142 rdf:type schema:MonetaryGrant
143 sg:grant.4850315 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-41263-5
144 rdf:type schema:MonetaryGrant
145 sg:journal.1045337 schema:issn 2045-2322
146 schema:name Scientific Reports
147 rdf:type schema:Periodical
148 sg:pub.10.1038/nmicrobiol.2016.77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049117060
149 https://doi.org/10.1038/nmicrobiol.2016.77
150 rdf:type schema:CreativeWork
151 sg:pub.10.1038/s41564-018-0203-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1105584555
152 https://doi.org/10.1038/s41564-018-0203-x
153 rdf:type schema:CreativeWork
154 sg:pub.10.1186/1471-2164-13-670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041430742
155 https://doi.org/10.1186/1471-2164-13-670
156 rdf:type schema:CreativeWork
157 https://doi.org/10.1002/9780471729259.mc07b02s9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016659008
158 rdf:type schema:CreativeWork
159 https://doi.org/10.1016/j.cell.2008.04.040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052593185
160 rdf:type schema:CreativeWork
161 https://doi.org/10.1016/j.jmb.2008.10.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028195096
162 rdf:type schema:CreativeWork
163 https://doi.org/10.1016/j.mib.2012.10.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035716056
164 rdf:type schema:CreativeWork
165 https://doi.org/10.1016/j.tim.2008.02.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003339222
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1016/s0022-2836(02)01031-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004417516
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1016/s0076-6879(10)71001-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037340173
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1038/emboj.2010.114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005616601
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1038/msb4100203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049071602
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1038/sj.emboj.7601877 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049206759
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1073/pnas.0609567103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000562234
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1073/pnas.95.10.5752 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025735342
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1074/jbc.m408855200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030331288
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1099/mic.0.000092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060393999
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1099/mic.0.036137-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060394781
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1111/1758-2229.12297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036658810
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1111/j.1365-2958.2006.05081.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017001124
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1111/j.1365-2958.2008.06238.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019917502
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1111/j.1365-2958.2008.06426.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028925250
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1126/science.1093027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046192024
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1128/aem.00640-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049436764
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1128/jb.00224-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032623020
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1128/jb.00914-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044438575
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1128/jb.01942-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020614923
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1128/jb.02095-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008403597
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1146/annurev-micro-092611-150039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026512129
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1146/annurev.biochem.69.1.183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042694911
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1146/annurev.micro.091208.073214 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019793505
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1146/annurev.micro.091208.073346 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042950995
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1371/journal.pbio.1000306 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010887892
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1371/journal.pbio.1000430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037672662
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1371/journal.pgen.1001220 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044040081
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1371/journal.pgen.1002872 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052379954
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1371/journal.pgen.1002951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006605306
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1371/journal.pgen.1004253 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018384224
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1371/journal.pone.0015628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016770500
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1371/journal.pone.0079759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008597050
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1371/journal.ppat.1002493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046858275
232 rdf:type schema:CreativeWork
233 https://www.grid.ac/institutes/grid.4563.4 schema:alternateName University of Nottingham
234 schema:name Northern Technical University, Mosul, Iraq
235 School of Life Sciences, University of Nottingham, Medical School, Nottingham, United Kingdom
236 rdf:type schema:Organization
237 https://www.grid.ac/institutes/grid.8391.3 schema:alternateName University of Exeter
238 schema:name Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, United Kingdom
239 School of Life Sciences, University of Nottingham, Medical School, Nottingham, United Kingdom
240 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...