Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Pascal Fenske, M. Katharina Grauel, Marisa M. Brockmann, Anja L. Dorrn, Thorsten Trimbuch, Christian Rosenmund

ABSTRACT

Recently developed technology to differentiate induced pluripotent stem cells (iPSCs) into human induced neurons (iNs) provides an exciting opportunity to study the function of human neurons. However, functional characterisations of iNs have been hampered by the reliance on mass culturing protocols which do not allow assessment of synaptic release characteristics and neuronal morphology at the individual cell level with quantitative precision. Here, we have developed for the first time a protocol to generate autaptic cultures of iPSC-derived iNs. We show that our method efficiently generates mature, autaptic iNs with robust spontaneous and action potential-driven synaptic transmission. The synaptic responses are sensitive to modulation by metabotropic receptor agonists as well as potentiation by acute phorbol ester application. Finally, we demonstrate loss of evoked and spontaneous release by Unc13A knockdown. This culture system provides a versatile platform allowing for quantitative and integrative assessment of morphophysiological and molecular parameters underlying human synaptic transmission. More... »

PAGES

4890

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-41259-1

DOI

http://dx.doi.org/10.1038/s41598-019-41259-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112880856

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30894602


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