Evidence for Escherichia coli DcuD carrier dependent FOF1-ATPase activity during fermentation of glycerol View Full Text


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Article Info

DATE

2019-12

AUTHORS

L. Karapetyan, A. Valle, J. Bolivar, A. Trchounian, K. Trchounian

ABSTRACT

During fermentation Escherichia coli excrete succinate mainly via Dcu family carriers. Current work reveals the total and N,N'-dicyclohexylcarbodiimide (DCCD) inhibited ATPase activity at pH 7.5 and 5.5 in E. coli wild type and dcu mutants upon glycerol fermentation. The overall ATPase activity was highest at pH 7.5 in dcuABCD mutant. In wild type cells 50% of the activity came from the FOF1-ATPase but in dcuD mutant it reached ~80%. K+ (100 mM) stimulate total but not DCCD inhibited ATPase activity 40% and 20% in wild type and dcuD mutant, respectively. 90% of overall ATPase activity was inhibited by DCCD at pH 5.5 only in dcuABC mutant. At pH 7.5 the H+ fluxes in E. coli wild type, dcuD and dcuABCD mutants was similar but in dcuABC triple mutant the H+ flux decreased 1.4 fold reaching 1.15 mM/min when glycerol was supplemented. In succinate assays the H+ flux was higher in the strains where DcuD is absent. No significant differences were determined in wild type and mutants specific growth rate except dcuD strain. Taken together it is suggested that during glycerol fermentation DcuD has impact on H+ fluxes, FOF1-ATPase activity and depends on potassium ions. More... »

PAGES

4279

References to SciGraph publications

  • 2013-06. Hydrogenase activity and proton-motive force generation by Escherichia coli during glycerol fermentation in JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
  • 1991-12. Foundations of vectorial metabolism and osmochemistry in BIOSCIENCE REPORTS
  • 1994-10. Relationship between the F0F1-ATPase and the K+-transport system within the membrane of anaerobically grownEscherichia coli. N,N′-dicyclohexylcarbodiimide-sensitive ATPase activity in mutants with defects in K+-transport in JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
  • 2014-03. Activating C4-dicarboxylate transporters DcuB and DcuC for improving succinate production in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1998-06. Relationship of the Escherichia coli TrkA System of Potassium Ion Uptake with the F0F1-ATPase Under Growth Conditions Without Anaerobic or Aerobic Respiration in BIOSCIENCE REPORTS
  • 1999-09. The dcuD (former yhcL) gene product of Escherichia coli as a member of the DcuC family of C4-dicarboxylate carriers: lack of evident expression in ARCHIVES OF MICROBIOLOGY
  • 2010-09. Hydrogen production by recombinant strains of Rhodobacter sphaeroides using a modified photosynthetic apparatus in APPLIED BIOCHEMISTRY AND MICROBIOLOGY
  • 2002-08. F0 Cysteine, bCys21, in the Escherichia coli ATP Synthase Is Involved in Regulation of Potassium Uptake and Molecular Hydrogen Production in Anaerobic Conditions in BIOSCIENCE REPORTS
  • 2011-12. Dependence on the F0F1-ATP synthase for the activities of the hydrogen-oxidizing hydrogenases 1 and 2 during glucose and glycerol fermentation at high and low pH in Escherichia coli in JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-41044-0

    DOI

    http://dx.doi.org/10.1038/s41598-019-41044-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112706577

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30862913


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