Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Toru Matsu-ura, Hideki Shirakawa, Kenichi G. N. Suzuki, Akitoshi Miyamoto, Kotomi Sugiura, Takayuki Michikawa, Akihiro Kusumi, Katsuhiko Mikoshiba

ABSTRACT

In most species, fertilization induces Ca2+ transients in the egg. In mammals, the Ca2+ rises are triggered by phospholipase Cζ (PLCζ) released from the sperm; IP3 generated by PLCζ induces Ca2+ release from the intracellular Ca2+ store through IP3 receptor, termed IP3-induced Ca2+ release. Here, we developed new fluorescent IP3 sensors (IRIS-2s) with the wider dynamic range and higher sensitivity (Kd = 0.047-1.7 μM) than that we developed previously. IRIS-2s employed green fluorescent protein and Halo-protein conjugated with the tetramethylrhodamine ligand as fluorescence resonance energy transfer (FRET) donor and acceptor, respectively. For simultaneous imaging of Ca2+ and IP3, using IRIS-2s as the IP3 sensor, we developed a new single fluorophore Ca2+ sensor protein, DYC3.60. With IRIS-2s and DYC3.60, we found that, right after fertilization, IP3 concentration ([IP3]) starts to increase before the onset of the first Ca2+ wave. [IP3] stayed at the elevated level with small peaks followed after Ca2+ spikes through Ca2+ oscillations. We detected delays in the peak of [IP3] compared to the peak of each Ca2+ spike, suggesting that Ca2+-induced regenerative IP3 production through PLC produces small [IP3] rises to maintain [IP3] over the basal level, which results in long lasting Ca2+ oscillations in fertilized eggs. More... »

PAGES

4829

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-40931-w

DOI

http://dx.doi.org/10.1038/s41598-019-40931-w

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112855306

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30886280


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-40931-w'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-40931-w'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-40931-w'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-40931-w'


 

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289 rdf:type schema:Organization
290 https://www.grid.ac/institutes/grid.440637.2 schema:alternateName ShanghaiTech University
291 schema:name Department of Pharmacology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, Shnjukuku, Tokyo, Japan
292 Laboratory for Developmental Neurobiology, Center for Brain Sciences, RIKEN, 2-1 Hirosawa, 351-0198, Wako, Saitama, Japan
293 Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 201210, Shanghai, China
294 rdf:type schema:Organization
295 https://www.grid.ac/institutes/grid.7597.c schema:alternateName RIKEN
296 schema:name Laboratory for Biotechnological Optics Research, Center for Advanced Photonics, RIKEN, 2-1 Hirosawa, 351-0198, Wako, Saitama, Japan
297 Laboratory for Developmental Neurobiology, Center for Brain Sciences, RIKEN, 2-1 Hirosawa, 351-0198, Wako, Saitama, Japan
298 rdf:type schema:Organization
 




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