Genome-wide Identification of Tebufenozide Resistant Genes in the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Miwa Uchibori-Asano, Akiya Jouraku, Toru Uchiyama, Kakeru Yokoi, Gaku Akiduki, Yoshitaka Suetsugu, Tetsuya Kobayashi, Akihito Ozawa, Saki Minami, Chiharu Ishizuka, Yoshiaki Nakagawa, Takaaki Daimon, Tetsuro Shinoda

ABSTRACT

The smaller tea tortrix, Adoxophyes honmai, has developed strong resistance to tebufenozide, a diacylhydrazine-type (DAH) insecticide. Here, we investigated its mechanism by identifying genes responsible for the tebufenozide resistance using various next generation sequencing techniques. First, double-digest restriction site-associated DNA sequencing (ddRAD-seq) identified two candidate loci. Then, synteny analyses using A. honmai draft genome sequences revealed that one locus contained the ecdysone receptor gene (EcR) and the other multiple CYP9A subfamily P450 genes. RNA-seq and direct sequencing of EcR cDNAs found a single nucleotide polymorphism (SNP), which was tightly linked to tebufenozide resistance and generated an amino acid substitution in the ligand-binding domain. The binding affinity to tebufenozide was about 4 times lower in in vitro translated EcR of the resistant strain than in the susceptible strain. RNA-seq analyses identified commonly up-regulated genes in resistant strains, including CYP9A and choline/carboxylesterase (CCE) genes. RT-qPCR analysis and bioassays showed that the expression levels of several CYP9A and CCE genes were moderately correlated with tebufenozide resistance. Collectively, these results suggest that the reduced binding affinity of EcR is the main factor and the enhanced detoxification activity by some CYP9As and CCEs plays a supplementary role in tebufenozide resistance in A. honmai. More... »

PAGES

4203

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-40863-5

DOI

http://dx.doi.org/10.1038/s41598-019-40863-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112706537

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30862839


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