Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Roby P. Bhattacharyya, Mark Walker, Rich Boykin, Sophie S. Son, Jamin Liu, Austin C. Hachey, Peijun Ma, Lidan Wu, Kyungyong Choi, Kaelyn C. Cummins, Maura Benson, Jennifer Skerry, Hyunryul Ryu, Sharon Y. Wong, Marcia B. Goldberg, Jongyoon Han, Virginia M. Pierce, Lisa A. Cosimi, Noam Shoresh, Jonathan Livny, Joseph Beechem, Deborah T. Hung

ABSTRACT

Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phylogenetic content of ribosomal RNA in a rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this assay identified bacteria with >89% accuracy at the species level and 100% accuracy at the family level, enabling all critical clinical distinctions. In pilot studies on primary clinical specimens, including sputum, blood cultures, and pus, bacteria from 5 different phyla were identified. More... »

PAGES

4516

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-40792-3

DOI

http://dx.doi.org/10.1038/s41598-019-40792-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112759325

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30872641


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