Poly-L-Lysine functionalised MWCNT-rGO nanosheets based 3-d hybrid structure for femtomolar level cholesterol detection using cantilever based sensing platform View Full Text


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Article Info

DATE

2019-12

AUTHORS

Aviru Kumar Basu, Amar Nath Sah, Asima Pradhan, Shantanu Bhattacharya

ABSTRACT

In this work we have developed a novel rGO-MWCNT (reduced graphene oxide-multiwalled carbon nanotube) nanocomposite material with Poly-L-Lysine functionalization which can be used for detection of biomolecules with enhanced sensitivity. The reduced GO sheets are found to play a major role as a connector and helps in the assembly of bundles of carbon nanotubes (CNTs) which may sometime play a role of upstanding nanostructures. The overall composite structure is further fully functionalized resulting in an overall high density of amino groups that can be used to capture biomolecules. The sensitivity of the as synthesized film is tested by the oxidation of cholesterol through cholesterol oxidase enzyme that is biochemically immobilized over these composite films. The test for the immobilization density of the novel films are carried out by mounting these films on sensitive thin section static micro/nano-cantilever platforms. The platforms have capability to measure cholesterol traces in blood upto an extent of 100 femto molar through deflection /bending of the cantilevers due to surface reaction. The films developed show a promise of high immobilization density which is further confirmed through fluorescence studies using FITC labeling of functionalized MWCNT-PLL and rGO-PLL films respectively. More... »

PAGES

3686

References to SciGraph publications

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  • 2007-03. The rise of graphene in NATURE MATERIALS
  • Journal

    TITLE

    Scientific Reports

    ISSUE

    1

    VOLUME

    9

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-40259-5

    DOI

    http://dx.doi.org/10.1038/s41598-019-40259-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112579002

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30842576


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