Metabolomic study of saxitoxin analogues and biosynthetic intermediates in dinoflagellates using 15N-labelled sodium nitrate as a nitrogen source View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Yuko Cho, Shigeki Tsuchiya, Takuo Omura, Kazuhiko Koike, Hiroshi Oikawa, Keiichi Konoki, Yasukatsu Oshima, Mari Yotsu-Yamashita

ABSTRACT

A stable-isotope-labelling method using 15N-labelled sodium nitrate as a nitrogen source was developed for the toxic dinoflagellate Alexandrium catenella. The labelled saxitoxin analogues (STXs), their precursor, and the biosynthetic intermediates were analyzed by column-switching high-resolution hydrophilic interaction liquid chromatography with mass spectrometry. The low contents on Day 0, high 15N incorporation % of Int-C'2 and Int-E' suggested that their turn-over rates are high and that the sizes of the pool of these compounds are smaller than those of the other intermediates. The experimentally determined isotopomer distributions showed that arginine, Int-C'2, 11-hydroxy-Int-C'2, Int-E', GTX5, GTX4, C1, and C2, each existed as a combination of three populations that consisted of the non-labelled molecules and the labelled isotopomers representing molecules newly synthesized by incorporation of 15N assimilated from the medium with two different incorporation rates. The order of 15N incorporation % values of the labelled populations predicted by this model largely agreed with the proposed biosynthetic route. The stable-isotope-labelling method will be useful for understanding the complex mechanism of nitrogen flux in STX-producing dinoflagellates. More... »

PAGES

3460

References to SciGraph publications

Journal

TITLE

Scientific Reports

ISSUE

1

VOLUME

9

From Grant

  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-39708-y

    DOI

    http://dx.doi.org/10.1038/s41598-019-39708-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112543988

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30837523


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