Vangl2 interaction plays a role in the proteasomal degradation of Prickle2 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Tadahiro Nagaoka, Mikio Furuse, Toshihisa Ohtsuka, Kunihiro Tsuchida, Masashi Kishi

ABSTRACT

The PET and LIM domain-containing protein, Prickle, plays a key role in planar cell polarity (PCP) in Drosophila. It has been reported that mutations in the PRICKLE2 gene, which encodes one of the human orthologues of Prickle, are associated with human diseases such as epilepsy and autism spectrum disorder. To develop preventive and therapeutic strategies for these intractable diseases, we studied the regulation of Prickle2 protein levels in transfected HEK293T cells. Prickle2 levels were negatively regulated by a physical interaction with another PCP protein, Van Gogh-like 2 (Vangl2). The Vangl2-mediated reduction in Prickle2 levels was, at least in part, relieved by proteasome inhibitors or by functional inhibition of the Cullin-1 E3 ubiquitin ligase. Furthermore, the expression of Vangl2 enhanced the polyubiquitination of Prickle2. This ubiquitination was partially blocked by co-expression of a ubiquitin mutant, which cannot be polymerised through their Lys48 residue to induce target proteins toward proteasomal degradation. Together, these results suggest that Prickle2 is polyubiquitinated by the Vangl2 interaction in a Cullin-1-dependent manner to limit its expression levels. This regulation may play a role in the local and temporal fine-tuning of Prickle protein levels during PCP signal-dependent cellular behaviours. More... »

PAGES

2912

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-39642-z

DOI

http://dx.doi.org/10.1038/s41598-019-39642-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112396773

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30814664


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Fujita Health University", 
          "id": "https://www.grid.ac/institutes/grid.256115.4", 
          "name": [
            "Division for Therapies Against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, 470-1192, Toyoake, Aichi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nagaoka", 
        "givenName": "Tadahiro", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute for Physiological Sciences", 
          "id": "https://www.grid.ac/institutes/grid.467811.d", 
          "name": [
            "Division of Cell Structure, National Institute for Physiological Sciences, 444-8787, Okazaki, Aichi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Furuse", 
        "givenName": "Mikio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Yamanashi", 
          "id": "https://www.grid.ac/institutes/grid.267500.6", 
          "name": [
            "Department of Biochemistry, Faculty of Medicine, University of Yamanashi, 409-3898, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ohtsuka", 
        "givenName": "Toshihisa", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Fujita Health University", 
          "id": "https://www.grid.ac/institutes/grid.256115.4", 
          "name": [
            "Division for Therapies Against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, 470-1192, Toyoake, Aichi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsuchida", 
        "givenName": "Kunihiro", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Osaka Medical Center and Research Institute for Maternal and Child Health", 
          "id": "https://www.grid.ac/institutes/grid.416629.e", 
          "name": [
            "Neuroscience Laboratory, Research Institute, Nozaki Tokushukai Hospital, 574-0074, Daito, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kishi", 
        "givenName": "Masashi", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nature07884", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000149768", 
          "https://doi.org/10.1038/nature07884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07884", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000149768", 
          "https://doi.org/10.1038/nature07884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.45.28557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000304689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1146221", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000632466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.089656", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001301902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep12916", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001972093", 
          "https://doi.org/10.1038/srep12916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0610155104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002273494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.13.17.2315", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003317242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ajhg.2010.12.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003674245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2013.03.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006612651"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-genet-112414-055224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008221427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/geno.2000.6493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008584422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep06940", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010651609", 
          "https://doi.org/10.1038/srep06940"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/mp.2013.71", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010865039", 
          "https://doi.org/10.1038/mp.2013.71"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/12175", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013676929", 
          "https://doi.org/10.1038/12175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/12175", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013676929", 
          "https://doi.org/10.1038/12175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.02.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014247560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-cellbio-100814-125315", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015912129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13041-014-0079-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017534751", 
          "https://doi.org/10.1186/s13041-014-0079-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13041-014-0079-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017534751", 
          "https://doi.org/10.1186/s13041-014-0079-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1003654", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018307558"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80046-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021801973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)80284-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024335450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jeb.024067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025932479"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1460-9568.2006.04596.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027446244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1460-9568.2006.04596.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027446244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0031988", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031666836"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/cshperspect.a002964", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032722259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1999.0289", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033185696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.00526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036383308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdg424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038111704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/humu.21589", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041398099"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1005259", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043234730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/90081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043286296", 
          "https://doi.org/10.1038/90081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/90081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043286296", 
          "https://doi.org/10.1038/90081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2014.01.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044838648"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(05)99020-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045594563"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm.2015.11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045794129", 
          "https://doi.org/10.1038/nrm.2015.11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(03)00084-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048940170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2005.09.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049522015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2005.09.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049522015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neulet.2015.12.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051121929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.14.8.5501", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052972215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi101286d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055203577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi101286d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055203577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi902180m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055210593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi902180m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055210593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jb/mvr023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059746379"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.277.5331.1511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062557898"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7569905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062647535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrclinonc.2016.206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074199044", 
          "https://doi.org/10.1038/nrclinonc.2016.206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082865612", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083211132", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm.2017.11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084129730", 
          "https://doi.org/10.1038/nrm.2017.11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41467-018-05127-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105359781", 
          "https://doi.org/10.1038/s41467-018-05127-2"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "The PET and LIM domain-containing protein, Prickle, plays a key role in planar cell polarity (PCP) in Drosophila. It has been reported that mutations in the PRICKLE2 gene, which encodes one of the human orthologues of Prickle, are associated with human diseases such as epilepsy and autism spectrum disorder. To develop preventive and therapeutic strategies for these intractable diseases, we studied the regulation of Prickle2 protein levels in transfected HEK293T cells. Prickle2 levels were negatively regulated by a physical interaction with another PCP protein, Van Gogh-like 2 (Vangl2). The Vangl2-mediated reduction in Prickle2 levels was, at least in part, relieved by proteasome inhibitors or by functional inhibition of the Cullin-1 E3 ubiquitin ligase. Furthermore, the expression of Vangl2 enhanced the polyubiquitination of Prickle2. This ubiquitination was partially blocked by co-expression of a ubiquitin mutant, which cannot be polymerised through their Lys48 residue to induce target proteins toward proteasomal degradation. Together, these results suggest that Prickle2 is polyubiquitinated by the Vangl2 interaction in a Cullin-1-dependent manner to limit its expression levels. This regulation may play a role in the local and temporal fine-tuning of Prickle protein levels during PCP signal-dependent cellular behaviours.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-019-39642-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6823559", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Vangl2 interaction plays a role in the proteasomal degradation of Prickle2", 
    "pagination": "2912", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d62008543fee90d4658ce39ec8e366bb9a649be4982e0d2b05c788dba293a55e"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30814664"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-019-39642-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112396773"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-019-39642-z", 
      "https://app.dimensions.ai/details/publication/pub.1112396773"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000350_0000000350/records_77583_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-019-39642-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39642-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39642-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39642-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39642-z'


 

This table displays all metadata directly associated to this object as RDF triples.

252 TRIPLES      21 PREDICATES      76 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-019-39642-z schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Naa2ae49229c6447da7d357f117dfffae
4 schema:citation sg:pub.10.1038/12175
5 sg:pub.10.1038/90081
6 sg:pub.10.1038/mp.2013.71
7 sg:pub.10.1038/nature07884
8 sg:pub.10.1038/nrclinonc.2016.206
9 sg:pub.10.1038/nrm.2015.11
10 sg:pub.10.1038/nrm.2017.11
11 sg:pub.10.1038/s41467-018-05127-2
12 sg:pub.10.1038/srep06940
13 sg:pub.10.1038/srep12916
14 sg:pub.10.1186/s13041-014-0079-5
15 https://app.dimensions.ai/details/publication/pub.1082865612
16 https://app.dimensions.ai/details/publication/pub.1083211132
17 https://doi.org/10.1002/humu.21589
18 https://doi.org/10.1006/bbrc.1999.0289
19 https://doi.org/10.1006/geno.2000.6493
20 https://doi.org/10.1016/j.ajhg.2010.12.012
21 https://doi.org/10.1016/j.cell.2009.02.025
22 https://doi.org/10.1016/j.celrep.2014.01.044
23 https://doi.org/10.1016/j.febslet.2013.03.030
24 https://doi.org/10.1016/j.molcel.2005.09.014
25 https://doi.org/10.1016/j.neulet.2015.12.009
26 https://doi.org/10.1016/s0076-6879(05)99020-4
27 https://doi.org/10.1016/s0092-8674(00)80046-x
28 https://doi.org/10.1016/s0896-6273(00)80284-6
29 https://doi.org/10.1016/s0896-6273(03)00084-9
30 https://doi.org/10.1021/bi101286d
31 https://doi.org/10.1021/bi902180m
32 https://doi.org/10.1073/pnas.0610155104
33 https://doi.org/10.1074/jbc.272.45.28557
34 https://doi.org/10.1093/emboj/cdg424
35 https://doi.org/10.1093/jb/mvr023
36 https://doi.org/10.1101/cshperspect.a002964
37 https://doi.org/10.1101/gad.13.17.2315
38 https://doi.org/10.1111/j.1460-9568.2006.04596.x
39 https://doi.org/10.1126/science.1146221
40 https://doi.org/10.1126/science.277.5331.1511
41 https://doi.org/10.1126/science.7569905
42 https://doi.org/10.1128/mcb.14.8.5501
43 https://doi.org/10.1146/annurev-cellbio-100814-125315
44 https://doi.org/10.1146/annurev-genet-112414-055224
45 https://doi.org/10.1242/dev.00526
46 https://doi.org/10.1242/dev.089656
47 https://doi.org/10.1242/jeb.024067
48 https://doi.org/10.1371/journal.pgen.1003654
49 https://doi.org/10.1371/journal.pgen.1005259
50 https://doi.org/10.1371/journal.pone.0031988
51 schema:datePublished 2019-12
52 schema:datePublishedReg 2019-12-01
53 schema:description The PET and LIM domain-containing protein, Prickle, plays a key role in planar cell polarity (PCP) in Drosophila. It has been reported that mutations in the PRICKLE2 gene, which encodes one of the human orthologues of Prickle, are associated with human diseases such as epilepsy and autism spectrum disorder. To develop preventive and therapeutic strategies for these intractable diseases, we studied the regulation of Prickle2 protein levels in transfected HEK293T cells. Prickle2 levels were negatively regulated by a physical interaction with another PCP protein, Van Gogh-like 2 (Vangl2). The Vangl2-mediated reduction in Prickle2 levels was, at least in part, relieved by proteasome inhibitors or by functional inhibition of the Cullin-1 E3 ubiquitin ligase. Furthermore, the expression of Vangl2 enhanced the polyubiquitination of Prickle2. This ubiquitination was partially blocked by co-expression of a ubiquitin mutant, which cannot be polymerised through their Lys48 residue to induce target proteins toward proteasomal degradation. Together, these results suggest that Prickle2 is polyubiquitinated by the Vangl2 interaction in a Cullin-1-dependent manner to limit its expression levels. This regulation may play a role in the local and temporal fine-tuning of Prickle protein levels during PCP signal-dependent cellular behaviours.
54 schema:genre research_article
55 schema:inLanguage en
56 schema:isAccessibleForFree true
57 schema:isPartOf N73efd23c671647b0b999ddf27fe88b05
58 N9ccd1fcca7e9460cb1a9e4dcad2eee76
59 sg:journal.1045337
60 schema:name Vangl2 interaction plays a role in the proteasomal degradation of Prickle2
61 schema:pagination 2912
62 schema:productId N2dfdc55b0d9447aabd1deb92afc76fbc
63 N58d6f83b0a534006b8342a8fd9bbfc3f
64 N87812cbba03d4cc4b5a10d57de88ced0
65 Nfc0fe5bc2dc74c948958f47bb540d6c2
66 Nfedf0537623747ee99279f091ff7f439
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112396773
68 https://doi.org/10.1038/s41598-019-39642-z
69 schema:sdDatePublished 2019-04-11T10:50
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher N0337cbbbbf2c45a182b4e28ebf77dc85
72 schema:url https://www.nature.com/articles/s41598-019-39642-z
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N00380c7c1c1b442d8c85fa3cee19946a schema:affiliation https://www.grid.ac/institutes/grid.256115.4
77 schema:familyName Nagaoka
78 schema:givenName Tadahiro
79 rdf:type schema:Person
80 N0337cbbbbf2c45a182b4e28ebf77dc85 schema:name Springer Nature - SN SciGraph project
81 rdf:type schema:Organization
82 N19b0c67140b6420580b68df59bb73bd3 schema:affiliation https://www.grid.ac/institutes/grid.416629.e
83 schema:familyName Kishi
84 schema:givenName Masashi
85 rdf:type schema:Person
86 N2dfdc55b0d9447aabd1deb92afc76fbc schema:name nlm_unique_id
87 schema:value 101563288
88 rdf:type schema:PropertyValue
89 N3e755242df9e418885732a064fe2380c rdf:first Ncbab7eef1dcd4b108aa76d6597eca58c
90 rdf:rest Nacaf2e4c6a7c4f5e86330013191c4cac
91 N58d6f83b0a534006b8342a8fd9bbfc3f schema:name readcube_id
92 schema:value d62008543fee90d4658ce39ec8e366bb9a649be4982e0d2b05c788dba293a55e
93 rdf:type schema:PropertyValue
94 N6807068eec8a49bfa6f7fff76667593e rdf:first N19b0c67140b6420580b68df59bb73bd3
95 rdf:rest rdf:nil
96 N6f003f15b1c74ce98b049b551d1f26bc schema:affiliation https://www.grid.ac/institutes/grid.467811.d
97 schema:familyName Furuse
98 schema:givenName Mikio
99 rdf:type schema:Person
100 N73efd23c671647b0b999ddf27fe88b05 schema:issueNumber 1
101 rdf:type schema:PublicationIssue
102 N87812cbba03d4cc4b5a10d57de88ced0 schema:name pubmed_id
103 schema:value 30814664
104 rdf:type schema:PropertyValue
105 N9ccd1fcca7e9460cb1a9e4dcad2eee76 schema:volumeNumber 9
106 rdf:type schema:PublicationVolume
107 Na0307ec29e6a44ccb3553ca3c159e597 schema:affiliation https://www.grid.ac/institutes/grid.256115.4
108 schema:familyName Tsuchida
109 schema:givenName Kunihiro
110 rdf:type schema:Person
111 Na69dc3e5c6dd4558a087cc59faa0e501 rdf:first N6f003f15b1c74ce98b049b551d1f26bc
112 rdf:rest N3e755242df9e418885732a064fe2380c
113 Naa2ae49229c6447da7d357f117dfffae rdf:first N00380c7c1c1b442d8c85fa3cee19946a
114 rdf:rest Na69dc3e5c6dd4558a087cc59faa0e501
115 Nacaf2e4c6a7c4f5e86330013191c4cac rdf:first Na0307ec29e6a44ccb3553ca3c159e597
116 rdf:rest N6807068eec8a49bfa6f7fff76667593e
117 Ncbab7eef1dcd4b108aa76d6597eca58c schema:affiliation https://www.grid.ac/institutes/grid.267500.6
118 schema:familyName Ohtsuka
119 schema:givenName Toshihisa
120 rdf:type schema:Person
121 Nfc0fe5bc2dc74c948958f47bb540d6c2 schema:name doi
122 schema:value 10.1038/s41598-019-39642-z
123 rdf:type schema:PropertyValue
124 Nfedf0537623747ee99279f091ff7f439 schema:name dimensions_id
125 schema:value pub.1112396773
126 rdf:type schema:PropertyValue
127 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
128 schema:name Biological Sciences
129 rdf:type schema:DefinedTerm
130 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
131 schema:name Biochemistry and Cell Biology
132 rdf:type schema:DefinedTerm
133 sg:grant.6823559 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-39642-z
134 rdf:type schema:MonetaryGrant
135 sg:journal.1045337 schema:issn 2045-2322
136 schema:name Scientific Reports
137 rdf:type schema:Periodical
138 sg:pub.10.1038/12175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013676929
139 https://doi.org/10.1038/12175
140 rdf:type schema:CreativeWork
141 sg:pub.10.1038/90081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043286296
142 https://doi.org/10.1038/90081
143 rdf:type schema:CreativeWork
144 sg:pub.10.1038/mp.2013.71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010865039
145 https://doi.org/10.1038/mp.2013.71
146 rdf:type schema:CreativeWork
147 sg:pub.10.1038/nature07884 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000149768
148 https://doi.org/10.1038/nature07884
149 rdf:type schema:CreativeWork
150 sg:pub.10.1038/nrclinonc.2016.206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074199044
151 https://doi.org/10.1038/nrclinonc.2016.206
152 rdf:type schema:CreativeWork
153 sg:pub.10.1038/nrm.2015.11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045794129
154 https://doi.org/10.1038/nrm.2015.11
155 rdf:type schema:CreativeWork
156 sg:pub.10.1038/nrm.2017.11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084129730
157 https://doi.org/10.1038/nrm.2017.11
158 rdf:type schema:CreativeWork
159 sg:pub.10.1038/s41467-018-05127-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105359781
160 https://doi.org/10.1038/s41467-018-05127-2
161 rdf:type schema:CreativeWork
162 sg:pub.10.1038/srep06940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010651609
163 https://doi.org/10.1038/srep06940
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/srep12916 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001972093
166 https://doi.org/10.1038/srep12916
167 rdf:type schema:CreativeWork
168 sg:pub.10.1186/s13041-014-0079-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017534751
169 https://doi.org/10.1186/s13041-014-0079-5
170 rdf:type schema:CreativeWork
171 https://app.dimensions.ai/details/publication/pub.1082865612 schema:CreativeWork
172 https://app.dimensions.ai/details/publication/pub.1083211132 schema:CreativeWork
173 https://doi.org/10.1002/humu.21589 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041398099
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1006/bbrc.1999.0289 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033185696
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1006/geno.2000.6493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008584422
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/j.ajhg.2010.12.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003674245
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/j.cell.2009.02.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014247560
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/j.celrep.2014.01.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044838648
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.febslet.2013.03.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006612651
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.molcel.2005.09.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049522015
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.neulet.2015.12.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051121929
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/s0076-6879(05)99020-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045594563
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/s0092-8674(00)80046-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1021801973
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/s0896-6273(00)80284-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024335450
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/s0896-6273(03)00084-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048940170
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1021/bi101286d schema:sameAs https://app.dimensions.ai/details/publication/pub.1055203577
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1021/bi902180m schema:sameAs https://app.dimensions.ai/details/publication/pub.1055210593
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1073/pnas.0610155104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002273494
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1074/jbc.272.45.28557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000304689
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1093/emboj/cdg424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038111704
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1093/jb/mvr023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059746379
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1101/cshperspect.a002964 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032722259
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1101/gad.13.17.2315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003317242
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1111/j.1460-9568.2006.04596.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027446244
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1126/science.1146221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000632466
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1126/science.277.5331.1511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062557898
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1126/science.7569905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062647535
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1128/mcb.14.8.5501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052972215
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1146/annurev-cellbio-100814-125315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015912129
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1146/annurev-genet-112414-055224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008221427
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1242/dev.00526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036383308
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1242/dev.089656 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001301902
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1242/jeb.024067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025932479
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1371/journal.pgen.1003654 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018307558
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1371/journal.pgen.1005259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043234730
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1371/journal.pone.0031988 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031666836
240 rdf:type schema:CreativeWork
241 https://www.grid.ac/institutes/grid.256115.4 schema:alternateName Fujita Health University
242 schema:name Division for Therapies Against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, 470-1192, Toyoake, Aichi, Japan
243 rdf:type schema:Organization
244 https://www.grid.ac/institutes/grid.267500.6 schema:alternateName University of Yamanashi
245 schema:name Department of Biochemistry, Faculty of Medicine, University of Yamanashi, 409-3898, Yamanashi, Japan
246 rdf:type schema:Organization
247 https://www.grid.ac/institutes/grid.416629.e schema:alternateName Osaka Medical Center and Research Institute for Maternal and Child Health
248 schema:name Neuroscience Laboratory, Research Institute, Nozaki Tokushukai Hospital, 574-0074, Daito, Osaka, Japan
249 rdf:type schema:Organization
250 https://www.grid.ac/institutes/grid.467811.d schema:alternateName National Institute for Physiological Sciences
251 schema:name Division of Cell Structure, National Institute for Physiological Sciences, 444-8787, Okazaki, Aichi, Japan
252 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...