Viscosin-like lipopeptides from frog skin bacteria inhibit Aspergillus fumigatus and Batrachochytrium dendrobatidis detected by imaging mass spectrometry and molecular networking View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-02-28

AUTHORS

Christian Martin H., Roberto Ibáñez, Louis-Félix Nothias, Cristopher A. Boya P., Laura K. Reinert, Louise A. Rollins-Smith, Pieter C. Dorrestein, Marcelino Gutiérrez

ABSTRACT

Amphibian populations worldwide have declined and in some cases become extinct due to chytridiomycosis, a pandemic disease caused by the fungus Batrachochytrium dendrobatidis; however, some species have survived these fungal epidemics. Previous studies have suggested that the resistance of these species is due to the presence of cutaneous bacteria producing antifungal metabolites. As our understanding of these metabolites is still limited, we assessed the potential of such compounds against human-relevant fungi such as Aspergillus. In this work we isolated 201 bacterial strains from fifteen samples belonging to seven frog species collected in the highlands of Panama and tested them against Aspergillus fumigatus. Among the 29 bacterial isolates that exhibited antifungal activity, Pseudomonas cichorii showed the greatest inhibition. To visualize the distribution of compounds and identify them in the inhibition zone produced by P. cichorii, we employed MALDI imaging mass spectrometry (MALDI IMS) and MS/MS molecular networking. We identified viscosin and massetolides A, F, G and H in the inhibition zone. Furthermore, viscosin was isolated and evaluated in vitro against A. fumigatus and B. dendrobatidis showing MIC values of 62.50 µg/mL and 31.25 µg/mL, respectively. This is the first report of cyclic depsipeptides with antifungal activity isolated from frog cutaneous bacteria. More... »

PAGES

3019

References to SciGraph publications

  • 2007-09-27. MALDI imaging mass spectrometry: molecular snapshots of biochemical systems in NATURE METHODS
  • 2011-04-28. Pharmacology and metabolism of anidulafungin, caspofungin and micafungin in the treatment of invasive candidosis - review of the literature in EUROPEAN JOURNAL OF MEDICAL RESEARCH
  • 2008-10-24. Amphibian Chemical Defense: Antifungal Metabolites of the Microsymbiont Janthinobacterium lividum on the Salamander Plethodon cinereus in JOURNAL OF CHEMICAL ECOLOGY
  • 2014-05-23. Amphibian skin may select for rare environmental microbes in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2016-08-09. Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking in NATURE BIOTECHNOLOGY
  • 2017-11-09. Prodigiosin, Violacein, and Volatile Organic Compounds Produced by Widespread Cutaneous Bacteria of Amphibians Can Inhibit Two Batrachochytrium Fungal Pathogens in MICROBIAL ECOLOGY
  • 2006-01-21. Amphibian Pathogen Batrachochytrium dendrobatidis Is Inhibited by the Cutaneous Bacteria of Amphibian Species in ECOHEALTH
  • 2013-07-11. Sequence similarity search, Multiple Sequence Alignment, Model Selection, Distance Matrix and Phylogeny Reconstruction in PROTOCOL EXCHANGE
  • 2012-08-23. Synergistic Inhibition of the Lethal Fungal Pathogen Batrachochytrium dendrobatidis: The Combined Effect of Symbiotic Bacterial Metabolites and Antimicrobial Peptides of the Frog Rana muscosa in JOURNAL OF CHEMICAL ECOLOGY
  • 1990-02. Surface active properties of viscosin: a peptidolipid antibiotic in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2011-09-21. The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia in LIPIDS IN HEALTH AND DISEASE
  • 2002-01. Antimicrobial peptides of multicellular organisms in NATURE
  • 1976-08. Cell wall formation in zoospores of Allomyces arbuscula in ARCHIVES OF MICROBIOLOGY
  • 2016-01-08. Skin bacterial diversity of Panamanian frogs is associated with host susceptibility and presence of Batrachochytrium dendrobatidis in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2016-10-31. Dereplication of peptidic natural products through database search of mass spectra in NATURE CHEMICAL BIOLOGY
  • 2017-07-17. Imaging mass spectrometry and MS/MS molecular networking reveals chemical interactions among cuticular bacteria and pathogenic fungi associated with fungus-growing ants in SCIENTIFIC REPORTS
  • 2016-10-31. Indexing the Pseudomonas specialized metabolome enabled the discovery of poaeamide B and the bananamides in NATURE MICROBIOLOGY
  • 2011-03. Motile Zoospores of Batrachochytrium dendrobatidis Move Away from Antifungal Metabolites Produced by Amphibian Skin Bacteria in ECOHEALTH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-019-39583-7

    DOI

    http://dx.doi.org/10.1038/s41598-019-39583-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112437048

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30816229


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Anura", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Aspergillus fumigatus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chytridiomycota", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lipopeptides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Peptides, Cyclic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pseudomonas", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Skin", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Symbiosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tandem Mass Spectrometry", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Biotechnology, Acharya Nagarjuna University, Guntur, India", 
              "id": "http://www.grid.ac/institutes/grid.411114.0", 
              "name": [
                "Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Cient\u00edficas y Servicios de Alta Tecnolog\u00eda (INDICASAT AIP), Panama, Republic of Panama", 
                "Department of Biotechnology, Acharya Nagarjuna University, Guntur, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Martin H.", 
            "givenName": "Christian", 
            "id": "sg:person.016510002125.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016510002125.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Departamento de Zoolog\u00eda, Universidad de Panam\u00e1, Panama, Republic of Panama", 
              "id": "http://www.grid.ac/institutes/grid.10984.34", 
              "name": [
                "Smithsonian Tropical Research Institute, Balboa, Ancon, Republic of Panama", 
                "Departamento de Zoolog\u00eda, Universidad de Panam\u00e1, Panama, Republic of Panama"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ib\u00e1\u00f1ez", 
            "givenName": "Roberto", 
            "id": "sg:person.01076314573.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076314573.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.266100.3", 
              "name": [
                "Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nothias", 
            "givenName": "Louis-F\u00e9lix", 
            "id": "sg:person.011720002155.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011720002155.41"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biotechnology, Acharya Nagarjuna University, Guntur, India", 
              "id": "http://www.grid.ac/institutes/grid.411114.0", 
              "name": [
                "Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Cient\u00edficas y Servicios de Alta Tecnolog\u00eda (INDICASAT AIP), Panama, Republic of Panama", 
                "Department of Biotechnology, Acharya Nagarjuna University, Guntur, India"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Boya P.", 
            "givenName": "Cristopher A.", 
            "id": "sg:person.011124333505.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011124333505.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology, Microbiology, and Immunology, and Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA", 
              "id": "http://www.grid.ac/institutes/grid.152326.1", 
              "name": [
                "Department of Pathology, Microbiology, and Immunology, and Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Reinert", 
            "givenName": "Laura K.", 
            "id": "sg:person.01243435636.86", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243435636.86"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA", 
              "id": "http://www.grid.ac/institutes/grid.152326.1", 
              "name": [
                "Department of Pathology, Microbiology, and Immunology, and Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA", 
                "Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rollins-Smith", 
            "givenName": "Louise A.", 
            "id": "sg:person.01031063540.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031063540.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA", 
              "id": "http://www.grid.ac/institutes/grid.266100.3", 
              "name": [
                "Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dorrestein", 
            "givenName": "Pieter C.", 
            "id": "sg:person.01023217043.95", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023217043.95"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Cient\u00edficas y Servicios de Alta Tecnolog\u00eda (INDICASAT AIP), Panama, Republic of Panama", 
              "id": "http://www.grid.ac/institutes/grid.452535.0", 
              "name": [
                "Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Cient\u00edficas y Servicios de Alta Tecnolog\u00eda (INDICASAT AIP), Panama, Republic of Panama"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guti\u00e9rrez", 
            "givenName": "Marcelino", 
            "id": "sg:person.01172553364.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172553364.19"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1186/2047-783x-16-4-159", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008492922", 
              "https://doi.org/10.1186/2047-783x-16-4-159"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10393-005-0009-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025177570", 
              "https://doi.org/10.1007/s10393-005-0009-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10886-012-0170-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011320538", 
              "https://doi.org/10.1007/s10886-012-0170-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2014.77", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039437053", 
              "https://doi.org/10.1038/ismej.2014.77"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1476-511x-10-161", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036346344", 
              "https://doi.org/10.1186/1476-511x-10-161"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmicrobiol.2016.197", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004804034", 
              "https://doi.org/10.1038/nmicrobiol.2016.197"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00425110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031083358", 
              "https://doi.org/10.1007/bf00425110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-05515-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1090637361", 
              "https://doi.org/10.1038/s41598-017-05515-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10886-008-9555-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043720487", 
              "https://doi.org/10.1007/s10886-008-9555-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00173720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047945096", 
              "https://doi.org/10.1007/bf00173720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.3597", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045197127", 
              "https://doi.org/10.1038/nbt.3597"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2015.234", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037667453", 
              "https://doi.org/10.1038/ismej.2015.234"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10393-011-0689-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016673834", 
              "https://doi.org/10.1007/s10393-011-0689-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1094", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026462539", 
              "https://doi.org/10.1038/nmeth1094"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/415389a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028850603", 
              "https://doi.org/10.1038/415389a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nchembio.2219", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043661254", 
              "https://doi.org/10.1038/nchembio.2219"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00248-017-1095-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092591735", 
              "https://doi.org/10.1007/s00248-017-1095-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/protex.2013.065", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056467170", 
              "https://doi.org/10.1038/protex.2013.065"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-02-28", 
        "datePublishedReg": "2019-02-28", 
        "description": "Amphibian populations worldwide have declined and in some cases become extinct due to chytridiomycosis, a pandemic disease caused by the fungus Batrachochytrium dendrobatidis; however, some species have survived these fungal epidemics. Previous studies have suggested that the resistance of these species is due to the presence of cutaneous bacteria producing antifungal metabolites. As our understanding of these metabolites is still limited, we assessed the potential of such compounds against human-relevant fungi such as Aspergillus. In this work we isolated 201 bacterial strains from fifteen samples belonging to seven frog species collected in the highlands of Panama and tested them against Aspergillus fumigatus. Among the 29 bacterial isolates that exhibited antifungal activity, Pseudomonas cichorii showed the greatest inhibition. To visualize the distribution of compounds and identify them in the inhibition zone produced by P. cichorii, we employed MALDI imaging mass spectrometry (MALDI IMS) and MS/MS molecular networking. We identified viscosin and massetolides A, F, G and H in the inhibition zone. Furthermore, viscosin was isolated and evaluated in vitro against A. fumigatus and B. dendrobatidis showing MIC values of 62.50\u2009\u00b5g/mL and 31.25\u2009\u00b5g/mL, respectively. This is the first report of cyclic depsipeptides with antifungal activity isolated from frog cutaneous bacteria.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/s41598-019-39583-7", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2349384", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.5540489", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "keywords": [
          "Batrachochytrium dendrobatidis", 
          "cutaneous bacteria", 
          "molecular networking", 
          "MS/MS molecular networking", 
          "fungus Batrachochytrium dendrobatidis", 
          "amphibian populations", 
          "fungal epidemics", 
          "frog species", 
          "P. cichorii", 
          "Pseudomonas cichorii", 
          "B. dendrobatidis", 
          "antifungal metabolites", 
          "dendrobatidis", 
          "antifungal activity", 
          "mass spectrometry", 
          "species", 
          "bacterial strains", 
          "cichorii", 
          "bacterial isolates", 
          "bacteria", 
          "cyclic depsipeptide", 
          "viscosin", 
          "A. fumigatus", 
          "skin bacteria", 
          "Aspergillus fumigatus", 
          "first report", 
          "fumigatus", 
          "chytridiomycosis", 
          "such compounds", 
          "fungi", 
          "metabolites", 
          "Panama", 
          "inhibition zone", 
          "lipopeptides", 
          "distribution of compounds", 
          "pandemic disease", 
          "previous studies", 
          "activity", 
          "greater inhibition", 
          "depsipeptide", 
          "Aspergillus", 
          "isolates", 
          "spectrometry", 
          "MALDI", 
          "inhibition", 
          "strains", 
          "MIC values", 
          "population", 
          "highlands", 
          "compounds", 
          "resistance", 
          "disease", 
          "understanding", 
          "zone", 
          "epidemic", 
          "presence", 
          "report", 
          "potential", 
          "mL", 
          "distribution", 
          "cases", 
          "study", 
          "samples", 
          "work", 
          "values", 
          "networking"
        ], 
        "name": "Viscosin-like lipopeptides from frog skin bacteria inhibit Aspergillus fumigatus and Batrachochytrium dendrobatidis detected by imaging mass spectrometry and molecular networking", 
        "pagination": "3019", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1112437048"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-019-39583-7"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30816229"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-019-39583-7", 
          "https://app.dimensions.ai/details/publication/pub.1112437048"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T21:05", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_815.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/s41598-019-39583-7"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39583-7'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39583-7'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39583-7'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39583-7'


     

    This table displays all metadata directly associated to this object as RDF triples.

    311 TRIPLES      21 PREDICATES      120 URIs      94 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-019-39583-7 schema:about N2bdf7e56323649c4bffc67d0c679763e
    2 N2c9c77e22c9f4274ae69270048ce2f14
    3 N8e7bfb212d784e46997031efe632627a
    4 N96ec3736b4364324a02d4e57c6c41c5a
    5 N9c9b1d016254485b8e26443a7a3474bf
    6 Nb99360ff4fc64bf8be09d8e369c4d5f3
    7 Nbe0d6cefa36344f49f409121c2b72e08
    8 Nc2fe9b56a2c9443a836065ba174ee9cc
    9 Ncad0584eaf5a45a591b30a3e761270b2
    10 Nd554f1f948544c85a3b0516d5671c568
    11 Nf9047be11e74481cb661efea3e808990
    12 anzsrc-for:06
    13 anzsrc-for:0605
    14 schema:author N86654525777a4c78a9b4b5f82f41c79b
    15 schema:citation sg:pub.10.1007/bf00173720
    16 sg:pub.10.1007/bf00425110
    17 sg:pub.10.1007/s00248-017-1095-7
    18 sg:pub.10.1007/s10393-005-0009-1
    19 sg:pub.10.1007/s10393-011-0689-7
    20 sg:pub.10.1007/s10886-008-9555-7
    21 sg:pub.10.1007/s10886-012-0170-2
    22 sg:pub.10.1038/415389a
    23 sg:pub.10.1038/ismej.2014.77
    24 sg:pub.10.1038/ismej.2015.234
    25 sg:pub.10.1038/nbt.3597
    26 sg:pub.10.1038/nchembio.2219
    27 sg:pub.10.1038/nmeth1094
    28 sg:pub.10.1038/nmicrobiol.2016.197
    29 sg:pub.10.1038/protex.2013.065
    30 sg:pub.10.1038/s41598-017-05515-6
    31 sg:pub.10.1186/1476-511x-10-161
    32 sg:pub.10.1186/2047-783x-16-4-159
    33 schema:datePublished 2019-02-28
    34 schema:datePublishedReg 2019-02-28
    35 schema:description Amphibian populations worldwide have declined and in some cases become extinct due to chytridiomycosis, a pandemic disease caused by the fungus Batrachochytrium dendrobatidis; however, some species have survived these fungal epidemics. Previous studies have suggested that the resistance of these species is due to the presence of cutaneous bacteria producing antifungal metabolites. As our understanding of these metabolites is still limited, we assessed the potential of such compounds against human-relevant fungi such as Aspergillus. In this work we isolated 201 bacterial strains from fifteen samples belonging to seven frog species collected in the highlands of Panama and tested them against Aspergillus fumigatus. Among the 29 bacterial isolates that exhibited antifungal activity, Pseudomonas cichorii showed the greatest inhibition. To visualize the distribution of compounds and identify them in the inhibition zone produced by P. cichorii, we employed MALDI imaging mass spectrometry (MALDI IMS) and MS/MS molecular networking. We identified viscosin and massetolides A, F, G and H in the inhibition zone. Furthermore, viscosin was isolated and evaluated in vitro against A. fumigatus and B. dendrobatidis showing MIC values of 62.50 µg/mL and 31.25 µg/mL, respectively. This is the first report of cyclic depsipeptides with antifungal activity isolated from frog cutaneous bacteria.
    36 schema:genre article
    37 schema:isAccessibleForFree true
    38 schema:isPartOf N867790ab5ead456b9658493cb476c5d9
    39 N9a50247873d748f89f0d92634a45a36f
    40 sg:journal.1045337
    41 schema:keywords A. fumigatus
    42 Aspergillus
    43 Aspergillus fumigatus
    44 B. dendrobatidis
    45 Batrachochytrium dendrobatidis
    46 MALDI
    47 MIC values
    48 MS/MS molecular networking
    49 P. cichorii
    50 Panama
    51 Pseudomonas cichorii
    52 activity
    53 amphibian populations
    54 antifungal activity
    55 antifungal metabolites
    56 bacteria
    57 bacterial isolates
    58 bacterial strains
    59 cases
    60 chytridiomycosis
    61 cichorii
    62 compounds
    63 cutaneous bacteria
    64 cyclic depsipeptide
    65 dendrobatidis
    66 depsipeptide
    67 disease
    68 distribution
    69 distribution of compounds
    70 epidemic
    71 first report
    72 frog species
    73 fumigatus
    74 fungal epidemics
    75 fungi
    76 fungus Batrachochytrium dendrobatidis
    77 greater inhibition
    78 highlands
    79 inhibition
    80 inhibition zone
    81 isolates
    82 lipopeptides
    83 mL
    84 mass spectrometry
    85 metabolites
    86 molecular networking
    87 networking
    88 pandemic disease
    89 population
    90 potential
    91 presence
    92 previous studies
    93 report
    94 resistance
    95 samples
    96 skin bacteria
    97 species
    98 spectrometry
    99 strains
    100 study
    101 such compounds
    102 understanding
    103 values
    104 viscosin
    105 work
    106 zone
    107 schema:name Viscosin-like lipopeptides from frog skin bacteria inhibit Aspergillus fumigatus and Batrachochytrium dendrobatidis detected by imaging mass spectrometry and molecular networking
    108 schema:pagination 3019
    109 schema:productId N2336727e1e7c403c8a639b61596e2754
    110 N95f2b5ee96ea45b2a9d428677fe8c5a4
    111 Nf763d86b460a477997100e277cabebe6
    112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112437048
    113 https://doi.org/10.1038/s41598-019-39583-7
    114 schema:sdDatePublished 2022-11-24T21:05
    115 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    116 schema:sdPublisher N8a117f0f056645c9b1c7a9da2756ece6
    117 schema:url https://doi.org/10.1038/s41598-019-39583-7
    118 sgo:license sg:explorer/license/
    119 sgo:sdDataset articles
    120 rdf:type schema:ScholarlyArticle
    121 N0a3320edc9ef4e8692d92683b59c3a4c rdf:first sg:person.01243435636.86
    122 rdf:rest Ne7a2c3f5056645b29aebfd4eed46bf37
    123 N2336727e1e7c403c8a639b61596e2754 schema:name dimensions_id
    124 schema:value pub.1112437048
    125 rdf:type schema:PropertyValue
    126 N2bdf7e56323649c4bffc67d0c679763e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Animals
    128 rdf:type schema:DefinedTerm
    129 N2c9c77e22c9f4274ae69270048ce2f14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Skin
    131 rdf:type schema:DefinedTerm
    132 N2ca04a289072420497dcc124de8cee0f rdf:first sg:person.01172553364.19
    133 rdf:rest rdf:nil
    134 N4f2f3cc676574ed08374783269a45e99 rdf:first sg:person.01023217043.95
    135 rdf:rest N2ca04a289072420497dcc124de8cee0f
    136 N86654525777a4c78a9b4b5f82f41c79b rdf:first sg:person.016510002125.57
    137 rdf:rest Nc4a44be702304974a3b4e4330d03be89
    138 N867790ab5ead456b9658493cb476c5d9 schema:issueNumber 1
    139 rdf:type schema:PublicationIssue
    140 N8a117f0f056645c9b1c7a9da2756ece6 schema:name Springer Nature - SN SciGraph project
    141 rdf:type schema:Organization
    142 N8e7bfb212d784e46997031efe632627a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Chytridiomycota
    144 rdf:type schema:DefinedTerm
    145 N95f2b5ee96ea45b2a9d428677fe8c5a4 schema:name pubmed_id
    146 schema:value 30816229
    147 rdf:type schema:PropertyValue
    148 N96ec3736b4364324a02d4e57c6c41c5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Aspergillus fumigatus
    150 rdf:type schema:DefinedTerm
    151 N9a50247873d748f89f0d92634a45a36f schema:volumeNumber 9
    152 rdf:type schema:PublicationVolume
    153 N9c9b1d016254485b8e26443a7a3474bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Lipopeptides
    155 rdf:type schema:DefinedTerm
    156 Nb99360ff4fc64bf8be09d8e369c4d5f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    157 schema:name Anura
    158 rdf:type schema:DefinedTerm
    159 Nbe0d6cefa36344f49f409121c2b72e08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
    161 rdf:type schema:DefinedTerm
    162 Nc2fe9b56a2c9443a836065ba174ee9cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    163 schema:name Symbiosis
    164 rdf:type schema:DefinedTerm
    165 Nc4a44be702304974a3b4e4330d03be89 rdf:first sg:person.01076314573.40
    166 rdf:rest Nd3591f7fcd954118bb85793991ae6e03
    167 Ncad0584eaf5a45a591b30a3e761270b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Pseudomonas
    169 rdf:type schema:DefinedTerm
    170 Nd3591f7fcd954118bb85793991ae6e03 rdf:first sg:person.011720002155.41
    171 rdf:rest Ndd29c94c86a44ef3ad842a4f0724fee3
    172 Nd554f1f948544c85a3b0516d5671c568 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    173 schema:name Tandem Mass Spectrometry
    174 rdf:type schema:DefinedTerm
    175 Ndd29c94c86a44ef3ad842a4f0724fee3 rdf:first sg:person.011124333505.52
    176 rdf:rest N0a3320edc9ef4e8692d92683b59c3a4c
    177 Ne7a2c3f5056645b29aebfd4eed46bf37 rdf:first sg:person.01031063540.75
    178 rdf:rest N4f2f3cc676574ed08374783269a45e99
    179 Nf763d86b460a477997100e277cabebe6 schema:name doi
    180 schema:value 10.1038/s41598-019-39583-7
    181 rdf:type schema:PropertyValue
    182 Nf9047be11e74481cb661efea3e808990 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Peptides, Cyclic
    184 rdf:type schema:DefinedTerm
    185 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    186 schema:name Biological Sciences
    187 rdf:type schema:DefinedTerm
    188 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
    189 schema:name Microbiology
    190 rdf:type schema:DefinedTerm
    191 sg:grant.2349384 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-39583-7
    192 rdf:type schema:MonetaryGrant
    193 sg:grant.5540489 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-019-39583-7
    194 rdf:type schema:MonetaryGrant
    195 sg:journal.1045337 schema:issn 2045-2322
    196 schema:name Scientific Reports
    197 schema:publisher Springer Nature
    198 rdf:type schema:Periodical
    199 sg:person.01023217043.95 schema:affiliation grid-institutes:grid.266100.3
    200 schema:familyName Dorrestein
    201 schema:givenName Pieter C.
    202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023217043.95
    203 rdf:type schema:Person
    204 sg:person.01031063540.75 schema:affiliation grid-institutes:grid.152326.1
    205 schema:familyName Rollins-Smith
    206 schema:givenName Louise A.
    207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01031063540.75
    208 rdf:type schema:Person
    209 sg:person.01076314573.40 schema:affiliation grid-institutes:grid.10984.34
    210 schema:familyName Ibáñez
    211 schema:givenName Roberto
    212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076314573.40
    213 rdf:type schema:Person
    214 sg:person.011124333505.52 schema:affiliation grid-institutes:grid.411114.0
    215 schema:familyName Boya P.
    216 schema:givenName Cristopher A.
    217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011124333505.52
    218 rdf:type schema:Person
    219 sg:person.011720002155.41 schema:affiliation grid-institutes:grid.266100.3
    220 schema:familyName Nothias
    221 schema:givenName Louis-Félix
    222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011720002155.41
    223 rdf:type schema:Person
    224 sg:person.01172553364.19 schema:affiliation grid-institutes:grid.452535.0
    225 schema:familyName Gutiérrez
    226 schema:givenName Marcelino
    227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172553364.19
    228 rdf:type schema:Person
    229 sg:person.01243435636.86 schema:affiliation grid-institutes:grid.152326.1
    230 schema:familyName Reinert
    231 schema:givenName Laura K.
    232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243435636.86
    233 rdf:type schema:Person
    234 sg:person.016510002125.57 schema:affiliation grid-institutes:grid.411114.0
    235 schema:familyName Martin H.
    236 schema:givenName Christian
    237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016510002125.57
    238 rdf:type schema:Person
    239 sg:pub.10.1007/bf00173720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047945096
    240 https://doi.org/10.1007/bf00173720
    241 rdf:type schema:CreativeWork
    242 sg:pub.10.1007/bf00425110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031083358
    243 https://doi.org/10.1007/bf00425110
    244 rdf:type schema:CreativeWork
    245 sg:pub.10.1007/s00248-017-1095-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092591735
    246 https://doi.org/10.1007/s00248-017-1095-7
    247 rdf:type schema:CreativeWork
    248 sg:pub.10.1007/s10393-005-0009-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025177570
    249 https://doi.org/10.1007/s10393-005-0009-1
    250 rdf:type schema:CreativeWork
    251 sg:pub.10.1007/s10393-011-0689-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016673834
    252 https://doi.org/10.1007/s10393-011-0689-7
    253 rdf:type schema:CreativeWork
    254 sg:pub.10.1007/s10886-008-9555-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043720487
    255 https://doi.org/10.1007/s10886-008-9555-7
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1007/s10886-012-0170-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011320538
    258 https://doi.org/10.1007/s10886-012-0170-2
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1038/415389a schema:sameAs https://app.dimensions.ai/details/publication/pub.1028850603
    261 https://doi.org/10.1038/415389a
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1038/ismej.2014.77 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039437053
    264 https://doi.org/10.1038/ismej.2014.77
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.1038/ismej.2015.234 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037667453
    267 https://doi.org/10.1038/ismej.2015.234
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1038/nbt.3597 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045197127
    270 https://doi.org/10.1038/nbt.3597
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1038/nchembio.2219 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043661254
    273 https://doi.org/10.1038/nchembio.2219
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1038/nmeth1094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026462539
    276 https://doi.org/10.1038/nmeth1094
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1038/nmicrobiol.2016.197 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004804034
    279 https://doi.org/10.1038/nmicrobiol.2016.197
    280 rdf:type schema:CreativeWork
    281 sg:pub.10.1038/protex.2013.065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056467170
    282 https://doi.org/10.1038/protex.2013.065
    283 rdf:type schema:CreativeWork
    284 sg:pub.10.1038/s41598-017-05515-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090637361
    285 https://doi.org/10.1038/s41598-017-05515-6
    286 rdf:type schema:CreativeWork
    287 sg:pub.10.1186/1476-511x-10-161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036346344
    288 https://doi.org/10.1186/1476-511x-10-161
    289 rdf:type schema:CreativeWork
    290 sg:pub.10.1186/2047-783x-16-4-159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008492922
    291 https://doi.org/10.1186/2047-783x-16-4-159
    292 rdf:type schema:CreativeWork
    293 grid-institutes:grid.10984.34 schema:alternateName Departamento de Zoología, Universidad de Panamá, Panama, Republic of Panama
    294 schema:name Departamento de Zoología, Universidad de Panamá, Panama, Republic of Panama
    295 Smithsonian Tropical Research Institute, Balboa, Ancon, Republic of Panama
    296 rdf:type schema:Organization
    297 grid-institutes:grid.152326.1 schema:alternateName Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA
    298 Department of Pathology, Microbiology, and Immunology, and Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    299 schema:name Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA
    300 Department of Pathology, Microbiology, and Immunology, and Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    301 rdf:type schema:Organization
    302 grid-institutes:grid.266100.3 schema:alternateName Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA
    303 schema:name Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA
    304 rdf:type schema:Organization
    305 grid-institutes:grid.411114.0 schema:alternateName Department of Biotechnology, Acharya Nagarjuna University, Guntur, India
    306 schema:name Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama
    307 Department of Biotechnology, Acharya Nagarjuna University, Guntur, India
    308 rdf:type schema:Organization
    309 grid-institutes:grid.452535.0 schema:alternateName Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama
    310 schema:name Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Panama, Republic of Panama
    311 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...