Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Magdalena C. E. Jochner, Junfeng An, Gisela Lättig-Tünnemann, Marieluise Kirchner, Alina Dagane, Gunnar Dittmar, Ulrich Dirnagl, Britta J. Eickholt, Christoph Harms

ABSTRACT

Phosphatase and tensin homolog (PTEN) signalling might influence neuronal survival after brain ischemia. However, the influence of the less studied longer variant termed PTEN-L (or PTENα) has not been studied to date. Therefore, we examined the translational variant PTEN-L in the context of neuronal survival. We identified PTEN-L by proteomics in murine neuronal cultures and brain lysates and established a novel model to analyse PTEN or PTEN-L variants independently in vitro while avoiding overexpression. We found that PTEN-L, unlike PTEN, localises predominantly in the cytosol and translocates to the nucleus 10-20 minutes after glutamate stress. Genomic ablation of PTEN and PTEN-L increased neuronal susceptibility to oxygen-glucose deprivation. This effect was rescued by expression of either PTEN-L indicating that both PTEN isoforms might contribute to a neuroprotective response. However, in direct comparison, PTEN-L replaced neurons were protected against ischemic-like stress compared to neurons expressing PTEN. Neurons expressing strictly nuclear PTEN-L NLS showed increased vulnerability, indicating that nuclear PTEN-L alone is not sufficient in protecting against stress. We identified mutually exclusive binding partners of PTEN-L or PTEN in cytosolic or nuclear fractions, which were regulated after ischemic-like stress. GRB2-associated-binding protein 2, which is known to interact with phosphoinositol-3-kinase, was enriched specifically with PTEN-L in the cytosol in proximity to the plasma membrane and their interaction was lost after glutamate exposure. The present study revealed that PTEN and PTEN-L have distinct functions in response to stress and might be involved in different mechanisms of neuroprotection. More... »

PAGES

3183

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-39438-1

DOI

http://dx.doi.org/10.1038/s41598-019-39438-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112441681

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30816308


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Berlin Institute of Health", 
          "id": "https://www.grid.ac/institutes/grid.484013.a", 
          "name": [
            "Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt Universit\u00e4t zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany", 
            "Center for Stroke Research Berlin, Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Berlin, Germany", 
            "Berlin Institute of Health (BIH), QUEST\u2013Centre for Transforming Biomedical Research, 10178 Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jochner", 
        "givenName": "Magdalena C. E.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "First Affiliated Hospital of Zhengzhou University", 
          "id": "https://www.grid.ac/institutes/grid.412633.1", 
          "name": [
            "Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt Universit\u00e4t zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany", 
            "Center for Stroke Research Berlin, Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Berlin, Germany", 
            "Medical Research Centre, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "An", 
        "givenName": "Junfeng", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charit\u00e9", 
          "id": "https://www.grid.ac/institutes/grid.6363.0", 
          "name": [
            "Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt Universit\u00e4t zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany", 
            "Center for Stroke Research Berlin, Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "L\u00e4ttig-T\u00fcnnemann", 
        "givenName": "Gisela", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Berlin Institute of Health", 
          "id": "https://www.grid.ac/institutes/grid.484013.a", 
          "name": [
            "Max Delbr\u00fcck Centre for Molecular Medicine (MDC), Proteomics Platform, Robert-R\u00f6ssle-Stra\u00dfe 10, 13125, Berlin, Germany", 
            "Berlin Institute of Health (BIH), Proteomics Platform, 10178 Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kirchner", 
        "givenName": "Marieluise", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine", 
          "id": "https://www.grid.ac/institutes/grid.419491.0", 
          "name": [
            "Max Delbr\u00fcck Centre for Molecular Medicine (MDC), Proteomics Platform, Robert-R\u00f6ssle-Stra\u00dfe 10, 13125, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dagane", 
        "givenName": "Alina", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Luxembourg Institute of Health", 
          "id": "https://www.grid.ac/institutes/grid.451012.3", 
          "name": [
            "Max Delbr\u00fcck Centre for Molecular Medicine (MDC), Proteomics Platform, Robert-R\u00f6ssle-Stra\u00dfe 10, 13125, Berlin, Germany", 
            "Proteome and Genome Research Laboratory, Luxembourg institute of Health, 1a Rue Thomas Edison, 1224, Strassen, Luxembourg"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dittmar", 
        "givenName": "Gunnar", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charit\u00e9", 
          "id": "https://www.grid.ac/institutes/grid.6363.0", 
          "name": [
            "Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt Universit\u00e4t zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany", 
            "Center for Stroke Research Berlin, Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Berlin, Germany", 
            "Berlin Institute of Health (BIH), QUEST\u2013Centre for Transforming Biomedical Research, 10178 Berlin, Germany", 
            "German Centre for Neurodegenerative Diseases (DZNE), Berlin, Germany", 
            "Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Institute of Biochemistry, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dirnagl", 
        "givenName": "Ulrich", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Charit\u00e9", 
          "id": "https://www.grid.ac/institutes/grid.6363.0", 
          "name": [
            "Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Institute of Biochemistry, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Eickholt", 
        "givenName": "Britta J.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Berlin Institute of Health", 
          "id": "https://www.grid.ac/institutes/grid.484013.a", 
          "name": [
            "Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt Universit\u00e4t zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany", 
            "Center for Stroke Research Berlin, Charit\u00e9\u2013Universit\u00e4tsmedizin Berlin, Berlin, Germany", 
            "Berlin Institute of Health (BIH), QUEST\u2013Centre for Transforming Biomedical Research, 10178 Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Harms", 
        "givenName": "Christoph", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.ymeth.2014.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000595513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2014.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000595513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cmet.2014.03.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001891527"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-05-0124", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002605958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jhep.2012.10.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006728619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1236188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008527759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1400737111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010078560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12031-014-0406-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011809083", 
          "https://doi.org/10.1007/s12031-014-0406-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0064802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012176157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.expneurol.2013.11.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013164568"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-015-8595-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013543171", 
          "https://doi.org/10.1007/s00216-015-8595-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng782", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013909587", 
          "https://doi.org/10.1038/ng782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng782", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013909587", 
          "https://doi.org/10.1038/ng782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1234907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016046009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1541-0420.2007.00905_13.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017185619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200505061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017615669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200505061", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017615669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-biochem-082411-113907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018149668"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1067081", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018333078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2006-01-029918", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023167929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mcn.2003.09.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025342841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpregu.00697.2009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026313050"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.00310-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026613453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026706357", 
          "https://doi.org/10.1038/nmeth.2019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.4428-15.2016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033147327"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/hep.25697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043130609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.11.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043484844"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuron.2007.05.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043623259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/syn.21725", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043669009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep20471", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044927205", 
          "https://doi.org/10.1038/srep20471"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045173751", 
          "https://doi.org/10.1038/nmeth.2075"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2011.03.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048419606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2007.261", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048442956", 
          "https://doi.org/10.1038/nprot.2007.261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049469421", 
          "https://doi.org/10.1038/nbt.1511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.5661-12.2013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050143984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(84)90457-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050880395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0000059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051280025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20150931", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052164466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20150931", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052164466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201105009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053353110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15548627.2015.1009767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1058406494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.21-14-05045.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074844441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.00357-16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079394190"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.13-08-03510.1993", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082651646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.21769/bioprotoc.2308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085903660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2017.05.088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086110974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41586-018-0139-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104171955", 
          "https://doi.org/10.1038/s41586-018-0139-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41586-018-0139-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104171955", 
          "https://doi.org/10.1038/s41586-018-0139-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41422-018-0056-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105038063", 
          "https://doi.org/10.1038/s41422-018-0056-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41422-018-0056-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105038063", 
          "https://doi.org/10.1038/s41422-018-0056-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/15548627.2018.1489477", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105306670"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Phosphatase and tensin homolog (PTEN) signalling might influence neuronal survival after brain ischemia. However, the influence of the less studied longer variant termed PTEN-L (or PTEN\u03b1) has not been studied to date. Therefore, we examined the translational variant PTEN-L in the context of neuronal survival. We identified PTEN-L by proteomics in murine neuronal cultures and brain lysates and established a novel model to analyse PTEN or PTEN-L variants independently in vitro while avoiding overexpression. We found that PTEN-L, unlike PTEN, localises predominantly in the cytosol and translocates to the nucleus 10-20\u2009minutes after glutamate stress. Genomic ablation of PTEN and PTEN-L increased neuronal susceptibility to oxygen-glucose deprivation. This effect was rescued by expression of either PTEN-L indicating that both PTEN isoforms might contribute to a neuroprotective response. However, in direct comparison, PTEN-L replaced neurons were protected against ischemic-like stress\u00a0compared to neurons expressing PTEN. Neurons expressing strictly nuclear PTEN-L NLS showed increased vulnerability, indicating that nuclear PTEN-L alone is not sufficient in protecting against stress. We identified mutually exclusive binding partners of PTEN-L or PTEN in cytosolic or nuclear fractions, which were regulated after ischemic-like stress. GRB2-associated-binding protein 2, which is known to interact with phosphoinositol-3-kinase, was enriched specifically with PTEN-L in the cytosol in proximity to the plasma membrane and their interaction was lost after glutamate exposure. The present study revealed\u00a0that PTEN and PTEN-L have distinct functions in response to stress and might be involved in different mechanisms of neuroprotection.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-019-39438-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress", 
    "pagination": "3183", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "efb9ae2ad98312ae88113cae6b8f040abb51798eeb4b9107c8e241a86044550f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30816308"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-019-39438-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112441681"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-019-39438-1", 
      "https://app.dimensions.ai/details/publication/pub.1112441681"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T11:05", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000352_0000000352/records_60367_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-019-39438-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39438-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39438-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39438-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-019-39438-1'


 

This table displays all metadata directly associated to this object as RDF triples.

283 TRIPLES      21 PREDICATES      74 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-019-39438-1 schema:about anzsrc-for:11
2 anzsrc-for:1109
3 schema:author Nc64f52da8f5443079e9d8e740f8d8f50
4 schema:citation sg:pub.10.1007/s00216-015-8595-4
5 sg:pub.10.1007/s12031-014-0406-4
6 sg:pub.10.1038/nbt.1511
7 sg:pub.10.1038/ng782
8 sg:pub.10.1038/nmeth.2019
9 sg:pub.10.1038/nmeth.2075
10 sg:pub.10.1038/nprot.2007.261
11 sg:pub.10.1038/s41422-018-0056-0
12 sg:pub.10.1038/s41586-018-0139-6
13 sg:pub.10.1038/srep20471
14 https://doi.org/10.1002/hep.25697
15 https://doi.org/10.1002/syn.21725
16 https://doi.org/10.1016/0092-8674(84)90457-4
17 https://doi.org/10.1016/j.cell.2006.11.040
18 https://doi.org/10.1016/j.celrep.2017.05.088
19 https://doi.org/10.1016/j.cmet.2014.03.023
20 https://doi.org/10.1016/j.expneurol.2013.11.017
21 https://doi.org/10.1016/j.jhep.2012.10.002
22 https://doi.org/10.1016/j.mcn.2003.09.012
23 https://doi.org/10.1016/j.neuron.2007.05.022
24 https://doi.org/10.1016/j.sbi.2011.03.011
25 https://doi.org/10.1016/j.ymeth.2014.10.005
26 https://doi.org/10.1042/bj20150931
27 https://doi.org/10.1073/pnas.1400737111
28 https://doi.org/10.1080/15548627.2015.1009767
29 https://doi.org/10.1080/15548627.2018.1489477
30 https://doi.org/10.1083/jcb.200505061
31 https://doi.org/10.1083/jcb.201105009
32 https://doi.org/10.1111/j.1541-0420.2007.00905_13.x
33 https://doi.org/10.1126/science.1067081
34 https://doi.org/10.1126/science.1234907
35 https://doi.org/10.1126/science.1236188
36 https://doi.org/10.1128/mcb.00310-08
37 https://doi.org/10.1128/mcb.00357-16
38 https://doi.org/10.1146/annurev-biochem-082411-113907
39 https://doi.org/10.1152/ajpregu.00697.2009
40 https://doi.org/10.1158/0008-5472.can-05-0124
41 https://doi.org/10.1182/blood-2006-01-029918
42 https://doi.org/10.1371/journal.pbio.0000059
43 https://doi.org/10.1371/journal.pone.0064802
44 https://doi.org/10.1523/jneurosci.13-08-03510.1993
45 https://doi.org/10.1523/jneurosci.21-14-05045.2001
46 https://doi.org/10.1523/jneurosci.4428-15.2016
47 https://doi.org/10.1523/jneurosci.5661-12.2013
48 https://doi.org/10.21769/bioprotoc.2308
49 schema:datePublished 2019-12
50 schema:datePublishedReg 2019-12-01
51 schema:description Phosphatase and tensin homolog (PTEN) signalling might influence neuronal survival after brain ischemia. However, the influence of the less studied longer variant termed PTEN-L (or PTENα) has not been studied to date. Therefore, we examined the translational variant PTEN-L in the context of neuronal survival. We identified PTEN-L by proteomics in murine neuronal cultures and brain lysates and established a novel model to analyse PTEN or PTEN-L variants independently in vitro while avoiding overexpression. We found that PTEN-L, unlike PTEN, localises predominantly in the cytosol and translocates to the nucleus 10-20 minutes after glutamate stress. Genomic ablation of PTEN and PTEN-L increased neuronal susceptibility to oxygen-glucose deprivation. This effect was rescued by expression of either PTEN-L indicating that both PTEN isoforms might contribute to a neuroprotective response. However, in direct comparison, PTEN-L replaced neurons were protected against ischemic-like stress compared to neurons expressing PTEN. Neurons expressing strictly nuclear PTEN-L NLS showed increased vulnerability, indicating that nuclear PTEN-L alone is not sufficient in protecting against stress. We identified mutually exclusive binding partners of PTEN-L or PTEN in cytosolic or nuclear fractions, which were regulated after ischemic-like stress. GRB2-associated-binding protein 2, which is known to interact with phosphoinositol-3-kinase, was enriched specifically with PTEN-L in the cytosol in proximity to the plasma membrane and their interaction was lost after glutamate exposure. The present study revealed that PTEN and PTEN-L have distinct functions in response to stress and might be involved in different mechanisms of neuroprotection.
52 schema:genre research_article
53 schema:inLanguage en
54 schema:isAccessibleForFree true
55 schema:isPartOf N86f015adefc64871af0c12e11ca32afc
56 Ne0d23bd724b8481c8a88644f09d60bd1
57 sg:journal.1045337
58 schema:name Unique properties of PTEN-L contribute to neuroprotection in response to ischemic-like stress
59 schema:pagination 3183
60 schema:productId N1d2a5422e7fe4d7884af493367445890
61 N69c7beb6095a403cb8afb7e90005b6eb
62 N83859a3fa1df416a85a53094ce0b3886
63 N9eee0802425242e58d7dafbbfcd322d8
64 Nb848ef8ba8c84058afd56ac3a6525362
65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112441681
66 https://doi.org/10.1038/s41598-019-39438-1
67 schema:sdDatePublished 2019-04-11T11:05
68 schema:sdLicense https://scigraph.springernature.com/explorer/license/
69 schema:sdPublisher N379cf7202aa249b8af96cee12512a427
70 schema:url https://www.nature.com/articles/s41598-019-39438-1
71 sgo:license sg:explorer/license/
72 sgo:sdDataset articles
73 rdf:type schema:ScholarlyArticle
74 N008166c9ad68464795b52b5e74773696 schema:affiliation https://www.grid.ac/institutes/grid.6363.0
75 schema:familyName Dirnagl
76 schema:givenName Ulrich
77 rdf:type schema:Person
78 N10aedfef98194dfa927679f0a782b9ae schema:affiliation https://www.grid.ac/institutes/grid.6363.0
79 schema:familyName Lättig-Tünnemann
80 schema:givenName Gisela
81 rdf:type schema:Person
82 N10bca2cee7754d81a5d01cccb3d1ac21 rdf:first N533e435f58744307a5aa36d97e63b735
83 rdf:rest N6d0cc0abdf25457eb4136605b849c69c
84 N1677c5a7831e45de84adff4c3af9e0d3 rdf:first N33f314c8782743eeb73d61c3c5e42481
85 rdf:rest rdf:nil
86 N1d2a5422e7fe4d7884af493367445890 schema:name readcube_id
87 schema:value efb9ae2ad98312ae88113cae6b8f040abb51798eeb4b9107c8e241a86044550f
88 rdf:type schema:PropertyValue
89 N2dee9d30758b4b949bedfbd54947cb4e rdf:first Ndaf10c2756aa4c6aa5a0e0c0c7b349d9
90 rdf:rest N1677c5a7831e45de84adff4c3af9e0d3
91 N33f314c8782743eeb73d61c3c5e42481 schema:affiliation https://www.grid.ac/institutes/grid.484013.a
92 schema:familyName Harms
93 schema:givenName Christoph
94 rdf:type schema:Person
95 N379cf7202aa249b8af96cee12512a427 schema:name Springer Nature - SN SciGraph project
96 rdf:type schema:Organization
97 N533e435f58744307a5aa36d97e63b735 schema:affiliation https://www.grid.ac/institutes/grid.419491.0
98 schema:familyName Dagane
99 schema:givenName Alina
100 rdf:type schema:Person
101 N69c7beb6095a403cb8afb7e90005b6eb schema:name pubmed_id
102 schema:value 30816308
103 rdf:type schema:PropertyValue
104 N6d0cc0abdf25457eb4136605b849c69c rdf:first Nb02ed99ee3b747b182c9cc04e06a300c
105 rdf:rest Nac18ee0e519743f5ae15b1f21a844932
106 N782032bb64b24e89a932c05a3ccb1e20 schema:affiliation https://www.grid.ac/institutes/grid.412633.1
107 schema:familyName An
108 schema:givenName Junfeng
109 rdf:type schema:Person
110 N83859a3fa1df416a85a53094ce0b3886 schema:name dimensions_id
111 schema:value pub.1112441681
112 rdf:type schema:PropertyValue
113 N86f015adefc64871af0c12e11ca32afc schema:issueNumber 1
114 rdf:type schema:PublicationIssue
115 N87ba93b0c9974151b2ca606566a789ed rdf:first N10aedfef98194dfa927679f0a782b9ae
116 rdf:rest N998a8354d15c43f5ac53ed21b28a3767
117 N998a8354d15c43f5ac53ed21b28a3767 rdf:first Na60d775bbf654b8da6636895575fc6ad
118 rdf:rest N10bca2cee7754d81a5d01cccb3d1ac21
119 N9993342f24294eab8db8f54a730db009 schema:affiliation https://www.grid.ac/institutes/grid.484013.a
120 schema:familyName Jochner
121 schema:givenName Magdalena C. E.
122 rdf:type schema:Person
123 N9eee0802425242e58d7dafbbfcd322d8 schema:name nlm_unique_id
124 schema:value 101563288
125 rdf:type schema:PropertyValue
126 Na60d775bbf654b8da6636895575fc6ad schema:affiliation https://www.grid.ac/institutes/grid.484013.a
127 schema:familyName Kirchner
128 schema:givenName Marieluise
129 rdf:type schema:Person
130 Nac18ee0e519743f5ae15b1f21a844932 rdf:first N008166c9ad68464795b52b5e74773696
131 rdf:rest N2dee9d30758b4b949bedfbd54947cb4e
132 Nb02ed99ee3b747b182c9cc04e06a300c schema:affiliation https://www.grid.ac/institutes/grid.451012.3
133 schema:familyName Dittmar
134 schema:givenName Gunnar
135 rdf:type schema:Person
136 Nb848ef8ba8c84058afd56ac3a6525362 schema:name doi
137 schema:value 10.1038/s41598-019-39438-1
138 rdf:type schema:PropertyValue
139 Nbffc110ebe734db4a4747c6dc6b4f5d0 rdf:first N782032bb64b24e89a932c05a3ccb1e20
140 rdf:rest N87ba93b0c9974151b2ca606566a789ed
141 Nc64f52da8f5443079e9d8e740f8d8f50 rdf:first N9993342f24294eab8db8f54a730db009
142 rdf:rest Nbffc110ebe734db4a4747c6dc6b4f5d0
143 Ndaf10c2756aa4c6aa5a0e0c0c7b349d9 schema:affiliation https://www.grid.ac/institutes/grid.6363.0
144 schema:familyName Eickholt
145 schema:givenName Britta J.
146 rdf:type schema:Person
147 Ne0d23bd724b8481c8a88644f09d60bd1 schema:volumeNumber 9
148 rdf:type schema:PublicationVolume
149 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
150 schema:name Medical and Health Sciences
151 rdf:type schema:DefinedTerm
152 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
153 schema:name Neurosciences
154 rdf:type schema:DefinedTerm
155 sg:journal.1045337 schema:issn 2045-2322
156 schema:name Scientific Reports
157 rdf:type schema:Periodical
158 sg:pub.10.1007/s00216-015-8595-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013543171
159 https://doi.org/10.1007/s00216-015-8595-4
160 rdf:type schema:CreativeWork
161 sg:pub.10.1007/s12031-014-0406-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011809083
162 https://doi.org/10.1007/s12031-014-0406-4
163 rdf:type schema:CreativeWork
164 sg:pub.10.1038/nbt.1511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049469421
165 https://doi.org/10.1038/nbt.1511
166 rdf:type schema:CreativeWork
167 sg:pub.10.1038/ng782 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013909587
168 https://doi.org/10.1038/ng782
169 rdf:type schema:CreativeWork
170 sg:pub.10.1038/nmeth.2019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026706357
171 https://doi.org/10.1038/nmeth.2019
172 rdf:type schema:CreativeWork
173 sg:pub.10.1038/nmeth.2075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045173751
174 https://doi.org/10.1038/nmeth.2075
175 rdf:type schema:CreativeWork
176 sg:pub.10.1038/nprot.2007.261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048442956
177 https://doi.org/10.1038/nprot.2007.261
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/s41422-018-0056-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105038063
180 https://doi.org/10.1038/s41422-018-0056-0
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/s41586-018-0139-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104171955
183 https://doi.org/10.1038/s41586-018-0139-6
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/srep20471 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044927205
186 https://doi.org/10.1038/srep20471
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1002/hep.25697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043130609
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1002/syn.21725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043669009
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/0092-8674(84)90457-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050880395
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.cell.2006.11.040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043484844
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.celrep.2017.05.088 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086110974
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.cmet.2014.03.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001891527
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.expneurol.2013.11.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013164568
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/j.jhep.2012.10.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006728619
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/j.mcn.2003.09.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025342841
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.neuron.2007.05.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043623259
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.sbi.2011.03.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048419606
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.ymeth.2014.10.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000595513
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1042/bj20150931 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052164466
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1073/pnas.1400737111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010078560
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1080/15548627.2015.1009767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058406494
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1080/15548627.2018.1489477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105306670
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1083/jcb.200505061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017615669
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1083/jcb.201105009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053353110
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1111/j.1541-0420.2007.00905_13.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017185619
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1126/science.1067081 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018333078
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1126/science.1234907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016046009
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1126/science.1236188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008527759
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1128/mcb.00310-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026613453
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1128/mcb.00357-16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079394190
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1146/annurev-biochem-082411-113907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018149668
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1152/ajpregu.00697.2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026313050
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1158/0008-5472.can-05-0124 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002605958
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1182/blood-2006-01-029918 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023167929
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1371/journal.pbio.0000059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051280025
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1371/journal.pone.0064802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012176157
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1523/jneurosci.13-08-03510.1993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082651646
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1523/jneurosci.21-14-05045.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074844441
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1523/jneurosci.4428-15.2016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033147327
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1523/jneurosci.5661-12.2013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050143984
255 rdf:type schema:CreativeWork
256 https://doi.org/10.21769/bioprotoc.2308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085903660
257 rdf:type schema:CreativeWork
258 https://www.grid.ac/institutes/grid.412633.1 schema:alternateName First Affiliated Hospital of Zhengzhou University
259 schema:name Center for Stroke Research Berlin, Charité–Universitätsmedizin Berlin, Berlin, Germany
260 Charité–Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany
261 Medical Research Centre, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
262 rdf:type schema:Organization
263 https://www.grid.ac/institutes/grid.419491.0 schema:alternateName Max Delbrück Center for Molecular Medicine
264 schema:name Max Delbrück Centre for Molecular Medicine (MDC), Proteomics Platform, Robert-Rössle-Straße 10, 13125, Berlin, Germany
265 rdf:type schema:Organization
266 https://www.grid.ac/institutes/grid.451012.3 schema:alternateName Luxembourg Institute of Health
267 schema:name Max Delbrück Centre for Molecular Medicine (MDC), Proteomics Platform, Robert-Rössle-Straße 10, 13125, Berlin, Germany
268 Proteome and Genome Research Laboratory, Luxembourg institute of Health, 1a Rue Thomas Edison, 1224, Strassen, Luxembourg
269 rdf:type schema:Organization
270 https://www.grid.ac/institutes/grid.484013.a schema:alternateName Berlin Institute of Health
271 schema:name Berlin Institute of Health (BIH), Proteomics Platform, 10178 Berlin, Germany
272 Berlin Institute of Health (BIH), QUEST–Centre for Transforming Biomedical Research, 10178 Berlin, Germany
273 Center for Stroke Research Berlin, Charité–Universitätsmedizin Berlin, Berlin, Germany
274 Charité–Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany
275 Max Delbrück Centre for Molecular Medicine (MDC), Proteomics Platform, Robert-Rössle-Straße 10, 13125, Berlin, Germany
276 rdf:type schema:Organization
277 https://www.grid.ac/institutes/grid.6363.0 schema:alternateName Charité
278 schema:name Berlin Institute of Health (BIH), QUEST–Centre for Transforming Biomedical Research, 10178 Berlin, Germany
279 Center for Stroke Research Berlin, Charité–Universitätsmedizin Berlin, Berlin, Germany
280 Charité–Universitätsmedizin Berlin, Institute of Biochemistry, Berlin, Germany
281 Charité–Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt Universität zu Berlin, and Berlin Institute of Health, Neurocure Cluster of Excellence, Department of Experimental Neurology, Berlin, Germany
282 German Centre for Neurodegenerative Diseases (DZNE), Berlin, Germany
283 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...