Functional expression of a peritrophin A-like SfPER protein is required for larval development in Spodoptera frugiperda (Lepidoptera: Noctuidae) View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Claudia Rodríguez-de la Noval, Lianet Rodríguez-Cabrera, Laurent Izquierdo, Luis A. Espinosa, Daily Hernandez, Milagro Ponce, Ivis Moran-Bertot, Pilar Tellez-Rodríguez, Orlando Borras-Hidalgo, Siliang Huang, Yunchao Kan, Denis J. Wright, Camilo Ayra-Pardo

ABSTRACT

Peritrophins are associated with structural and functional integrity of peritrophic membranes (PM), structures composed of chitin and proteins. PM lines the insect midgut and has roles in digestion and protection from toxins. We report the full-length cDNA cloning, molecular characterization and functional analysis of SfPER, a novel PM peritrophin A protein, in Spodoptera frugiperda. The predicted amino acid sequence indicated SfPER's domain structure as a CMCMC-type, consisting of a signal peptide and three chitin-binding (C) domains with two intervening mucin-like (M) domains. Phylogenetic analysis determined a close relationship between SfPER and another S. frugiperda PM peritrophin partial sequence. SfPER transcripts were found in larvae and adults but were absent from eggs and pupae. Chitin affinity studies with a recombinant SfPER-C1 peritrophin A-type domain fused to SUMO/His-tag confirmed that SfPER binds to chitin. Western blots of S. frugiperda larval proteins detected different sized variants of SfPER along the PM, with larger variants found towards the posterior PM. In vivo suppression of SfPER expression did not affect susceptibility of larvae to Bacillus thuringiensis toxin, but significantly decreased pupal weight and adult emergence, possibly due to PM structural alterations impairing digestion. Our results suggest SfPER could be a novel target for insect control. More... »

PAGES

2630

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-019-38734-0

DOI

http://dx.doi.org/10.1038/s41598-019-38734-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112308319

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30796291


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