Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Martina Raudenska, Monika Kratochvilova, Tomas Vicar, Jaromir Gumulec, Jan Balvan, Hana Polanska, Jan Pribyl, Michal Masarik

ABSTRACT

We focused on the biomechanical and morphological characteristics of prostate cancer cells and their changes resulting from the effect of docetaxel, cisplatin, and long-term zinc supplementation. Cell population surviving the treatment was characterized as follows: cell stiffness was assessed by atomic force microscopy, cell motility and invasion capacity were determined by colony forming assay, wound healing assay, coherence-controlled holographic microscopy, and real-time cell analysis. Cells of metastatic origin exhibited lower height than cells derived from the primary tumour. Cell dry mass and CAV1 gene expression followed similar trends as cell stiffness. Docetaxel- and cisplatin-surviving cells had higher stiffness, and decreased motility and invasive potential as compared to non-treated cells. This effect was not observed in zinc(II)-treated cells. We presume that cell stiffness changes may represent an important overlooked effect of cisplatin-based anti-cancer drugs. Atomic force microscopy and confocal microscopy data images used in our study are available for download in the Zenodo repository ( https://zenodo.org/ , Digital Object Identifiers:10.5281/zenodo.1494935). More... »

PAGES

1660

References to SciGraph publications

  • 2014. Nuclear Mechanics in Cancer in CANCER BIOLOGY AND THE NUCLEAR ENVELOPE
  • 2005-06. Chemistry in living systems in NATURE CHEMICAL BIOLOGY
  • 2007-12. Nanomechanical analysis of cells from cancer patients in NATURE NANOTECHNOLOGY
  • 1999-05. Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy in EUROPEAN BIOPHYSICS JOURNAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-38199-7

    DOI

    http://dx.doi.org/10.1038/s41598-018-38199-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1111978718

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30733487


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