Multifunctional Paper-Based Analytical Device for In Situ Cultivation and Screening of Escherichia coli Infections View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Julaluk Noiphung, Wanida Laiwattanapaisal

ABSTRACT

Point-of-care testing (POCT) for uropathogen detection and chemical screening has great benefits for the diagnosis of urinary tract infections (UTIs). The goal of this study was to develop a portable and inexpensive paper-based analytical device (PAD) for cultivating bacteria in situ and rapidly testing for nitrite on the same device. The PAD was fabricated using a wax printing technique to create a pattern on Whatman No. 1 filter paper, which was then combined with a cotton sheet to support bacterial growth. Nitrite detection was based on the principle of the Griess reaction, and a linear detection range of 0-1.6 mg/dL (R2 = 0.989) was obtained. Scanning electron microscopy (SEM) analysis demonstrated that the bacteria were able to grow and formed a cluster on the cellulose fibres within 2 hours. The enzyme β-glucuronidase, which is specifically produced by Escherichia coli, was able to convert the pre-immobilized 5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colourless substrate, generating a blue colour. Under optimum conditions, the proposed device allowed bacterial concentrations in the range of 104-107 colony forming units (CFU)/mL to be quantified within 6 hours. Moreover, the use of this device enables the identification of E. coli pathogens with selectivity in real urine samples. In conclusion, the PAD developed in this study for UTI screening provides a rapid, cost-effective diagnostic method for use in remote areas. More... »

PAGES

1555

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-38159-1

DOI

http://dx.doi.org/10.1038/s41598-018-38159-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111979635

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30733495


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chulalongkorn University", 
          "id": "https://www.grid.ac/institutes/grid.7922.e", 
          "name": [
            "Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Patumwan, Bangkok, Thailand"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Noiphung", 
        "givenName": "Julaluk", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chulalongkorn University", 
          "id": "https://www.grid.ac/institutes/grid.7922.e", 
          "name": [
            "Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Patumwan, Bangkok, Thailand", 
            "Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Chulalongkorn University, 10330, Bangkok, Thailand"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Laiwattanapaisal", 
        "givenName": "Wanida", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/nrmicro3432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001049530", 
          "https://doi.org/10.1038/nrmicro3432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bios.2015.07.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003110384"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.snb.2013.10.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004286201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mimet.2010.04.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005547358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep21342", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010148166", 
          "https://doi.org/10.1038/srep21342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mattod.2016.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012362872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/molbev/mst187", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012651735"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c6lc00895j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014972725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c2lc40391a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014976531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/00365540110080601", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015941065"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bios.2011.04.035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019619182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/383029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020621768"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro841", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024064458", 
          "https://doi.org/10.1038/nrmicro841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro841", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024064458", 
          "https://doi.org/10.1038/nrmicro841"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bios.2016.10.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024840598"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mpmed.2011.04.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025942418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c5lc01348h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026827333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c3lc50887k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026877477"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2013.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028083053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2013.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028083053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac203466y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030978411"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/2211068216680207", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034740601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/2211068216680207", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034740601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.talanta.2015.07.051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037109824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/ijerph7103657", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040035044"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mpmed.2007.05.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040066544"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1473-3099(13)70250-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040879612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.44.2.561-570.2006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041554033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/421997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042899633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-012-5975-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043970511", 
          "https://doi.org/10.1007/s00216-012-5975-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep18570", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044397192", 
          "https://doi.org/10.1038/srep18570"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep38171", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046125544", 
          "https://doi.org/10.1038/srep38171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.aca.2013.11.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046552458"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.juro.2010.09.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048607999"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00216-014-7985-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049479107", 
          "https://doi.org/10.1007/s00216-014-7985-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0018940", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049821957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c2lc21299d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050599888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.200603817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051066186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jchromb.2006.07.054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052384331"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c1lc20764d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053308701"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac5013249", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055016689"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac5019205", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055019475"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3238/arztebl.2010.0361", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078173606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078466592", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079179552", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14737159.2017.1285228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079396356"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080029689", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c6lc01450j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083844484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201702776", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085140525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/anie.201702776", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085140525"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bios.2017.05.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085180171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2471/blt.16.187468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091438015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(17)32130-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091764595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acs.analchem.7b04628", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1099725509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.aca.2018.01.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100810514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.aca.2018.01.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100810514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c8an00632f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104375240"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7150/thno.24941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1105066321"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Point-of-care testing (POCT) for uropathogen detection and chemical screening has great benefits for the diagnosis of urinary tract infections (UTIs). The goal of this study was to develop a portable and inexpensive paper-based analytical device (PAD) for cultivating bacteria in situ and rapidly testing for nitrite on the same device. The PAD was fabricated using a wax printing technique to create a pattern on Whatman No. 1 filter paper, which was then combined with a cotton sheet to support bacterial growth. Nitrite detection was based on the principle of the Griess reaction, and a linear detection range of 0-1.6\u2009mg/dL (R2\u2009=\u20090.989) was obtained. Scanning electron microscopy (SEM) analysis demonstrated that the bacteria were able to grow and formed a cluster on the cellulose fibres within 2\u2009hours. The enzyme \u03b2-glucuronidase, which is specifically produced by Escherichia coli, was able to convert the pre-immobilized 5-bromo-4-chloro-3-indolyl-\u03b2-D-glucuronide sodium salt (X-GlcA), a colourless substrate, generating a blue colour. Under optimum conditions, the proposed device allowed bacterial concentrations in the range of 104-107 colony forming units (CFU)/mL to be quantified within 6\u2009hours. Moreover, the use of this device enables the identification of E. coli pathogens with selectivity in real urine samples. In conclusion, the PAD developed in this study for UTI screening provides a rapid, cost-effective diagnostic method for use in remote areas.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-38159-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Multifunctional Paper-Based Analytical Device for In Situ Cultivation and Screening of Escherichia coli Infections", 
    "pagination": "1555", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "cb24b8fbbb8d972afe08a4187aa73dcf688364b30052704941d4262e728ae308"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30733495"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-38159-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111979635"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-38159-1", 
      "https://app.dimensions.ai/details/publication/pub.1111979635"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000334_0000000334/records_127800_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-38159-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38159-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38159-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38159-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38159-1'


 

This table displays all metadata directly associated to this object as RDF triples.

238 TRIPLES      21 PREDICATES      82 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-38159-1 schema:about anzsrc-for:11
2 anzsrc-for:1108
3 schema:author Ndda03b4e1d9148bebd11626d7c991683
4 schema:citation sg:pub.10.1007/s00216-012-5975-x
5 sg:pub.10.1007/s00216-014-7985-3
6 sg:pub.10.1038/nrmicro3432
7 sg:pub.10.1038/nrmicro841
8 sg:pub.10.1038/srep18570
9 sg:pub.10.1038/srep21342
10 sg:pub.10.1038/srep38171
11 https://app.dimensions.ai/details/publication/pub.1078466592
12 https://app.dimensions.ai/details/publication/pub.1079179552
13 https://app.dimensions.ai/details/publication/pub.1080029689
14 https://doi.org/10.1002/anie.200603817
15 https://doi.org/10.1002/anie.201702776
16 https://doi.org/10.1016/j.aca.2013.11.044
17 https://doi.org/10.1016/j.aca.2018.01.036
18 https://doi.org/10.1016/j.bios.2011.04.035
19 https://doi.org/10.1016/j.bios.2015.07.014
20 https://doi.org/10.1016/j.bios.2016.10.023
21 https://doi.org/10.1016/j.bios.2017.05.001
22 https://doi.org/10.1016/j.jchromb.2006.07.054
23 https://doi.org/10.1016/j.juro.2010.09.022
24 https://doi.org/10.1016/j.mattod.2016.07.001
25 https://doi.org/10.1016/j.mimet.2010.04.008
26 https://doi.org/10.1016/j.mpmed.2007.05.009
27 https://doi.org/10.1016/j.mpmed.2011.04.003
28 https://doi.org/10.1016/j.snb.2013.10.023
29 https://doi.org/10.1016/j.talanta.2015.07.051
30 https://doi.org/10.1016/j.ymeth.2013.07.001
31 https://doi.org/10.1016/s0140-6736(17)32130-x
32 https://doi.org/10.1016/s1473-3099(13)70250-0
33 https://doi.org/10.1021/ac203466y
34 https://doi.org/10.1021/ac5013249
35 https://doi.org/10.1021/ac5019205
36 https://doi.org/10.1021/acs.analchem.7b04628
37 https://doi.org/10.1039/c1lc20764d
38 https://doi.org/10.1039/c2lc21299d
39 https://doi.org/10.1039/c2lc40391a
40 https://doi.org/10.1039/c3lc50887k
41 https://doi.org/10.1039/c5lc01348h
42 https://doi.org/10.1039/c6lc00895j
43 https://doi.org/10.1039/c6lc01450j
44 https://doi.org/10.1039/c8an00632f
45 https://doi.org/10.1080/00365540110080601
46 https://doi.org/10.1080/14737159.2017.1285228
47 https://doi.org/10.1086/383029
48 https://doi.org/10.1086/421997
49 https://doi.org/10.1093/molbev/mst187
50 https://doi.org/10.1128/jcm.44.2.561-570.2006
51 https://doi.org/10.1177/2211068216680207
52 https://doi.org/10.1371/journal.pone.0018940
53 https://doi.org/10.2471/blt.16.187468
54 https://doi.org/10.3238/arztebl.2010.0361
55 https://doi.org/10.3390/ijerph7103657
56 https://doi.org/10.7150/thno.24941
57 schema:datePublished 2019-12
58 schema:datePublishedReg 2019-12-01
59 schema:description Point-of-care testing (POCT) for uropathogen detection and chemical screening has great benefits for the diagnosis of urinary tract infections (UTIs). The goal of this study was to develop a portable and inexpensive paper-based analytical device (PAD) for cultivating bacteria in situ and rapidly testing for nitrite on the same device. The PAD was fabricated using a wax printing technique to create a pattern on Whatman No. 1 filter paper, which was then combined with a cotton sheet to support bacterial growth. Nitrite detection was based on the principle of the Griess reaction, and a linear detection range of 0-1.6 mg/dL (R<sup>2</sup> = 0.989) was obtained. Scanning electron microscopy (SEM) analysis demonstrated that the bacteria were able to grow and formed a cluster on the cellulose fibres within 2 hours. The enzyme β-glucuronidase, which is specifically produced by Escherichia coli, was able to convert the pre-immobilized 5-bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt (X-GlcA), a colourless substrate, generating a blue colour. Under optimum conditions, the proposed device allowed bacterial concentrations in the range of 10<sup>4</sup>-10<sup>7</sup> colony forming units (CFU)/mL to be quantified within 6 hours. Moreover, the use of this device enables the identification of E. coli pathogens with selectivity in real urine samples. In conclusion, the PAD developed in this study for UTI screening provides a rapid, cost-effective diagnostic method for use in remote areas.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree true
63 schema:isPartOf N14c57779c1c94518b37571f404e39e9f
64 N23c809db5bf74cde813e1a117c9d9271
65 sg:journal.1045337
66 schema:name Multifunctional Paper-Based Analytical Device for In Situ Cultivation and Screening of Escherichia coli Infections
67 schema:pagination 1555
68 schema:productId N70004eeb82444348b8b3393256630c2c
69 N76eaefd1675541489f402923c1ff96af
70 N8f8746c83c214401a1b1e5764159c05c
71 Ncba900e358b945378a31d07f50aefe54
72 Nf0b67d2805de4b76a79126ee28667832
73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111979635
74 https://doi.org/10.1038/s41598-018-38159-1
75 schema:sdDatePublished 2019-04-11T09:04
76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
77 schema:sdPublisher Nc7a1c7e961874825b0bfacb311707cb0
78 schema:url https://www.nature.com/articles/s41598-018-38159-1
79 sgo:license sg:explorer/license/
80 sgo:sdDataset articles
81 rdf:type schema:ScholarlyArticle
82 N14c57779c1c94518b37571f404e39e9f schema:volumeNumber 9
83 rdf:type schema:PublicationVolume
84 N23c809db5bf74cde813e1a117c9d9271 schema:issueNumber 1
85 rdf:type schema:PublicationIssue
86 N34c83a05db3d4040bae7df8e5b75ebe0 rdf:first Nd6b2dfaf962b476aae74fbfdd443e2d1
87 rdf:rest rdf:nil
88 N636e720feaec405988618c1c67f5a446 schema:affiliation https://www.grid.ac/institutes/grid.7922.e
89 schema:familyName Noiphung
90 schema:givenName Julaluk
91 rdf:type schema:Person
92 N70004eeb82444348b8b3393256630c2c schema:name nlm_unique_id
93 schema:value 101563288
94 rdf:type schema:PropertyValue
95 N76eaefd1675541489f402923c1ff96af schema:name readcube_id
96 schema:value cb24b8fbbb8d972afe08a4187aa73dcf688364b30052704941d4262e728ae308
97 rdf:type schema:PropertyValue
98 N8f8746c83c214401a1b1e5764159c05c schema:name doi
99 schema:value 10.1038/s41598-018-38159-1
100 rdf:type schema:PropertyValue
101 Nc7a1c7e961874825b0bfacb311707cb0 schema:name Springer Nature - SN SciGraph project
102 rdf:type schema:Organization
103 Ncba900e358b945378a31d07f50aefe54 schema:name pubmed_id
104 schema:value 30733495
105 rdf:type schema:PropertyValue
106 Nd6b2dfaf962b476aae74fbfdd443e2d1 schema:affiliation https://www.grid.ac/institutes/grid.7922.e
107 schema:familyName Laiwattanapaisal
108 schema:givenName Wanida
109 rdf:type schema:Person
110 Ndda03b4e1d9148bebd11626d7c991683 rdf:first N636e720feaec405988618c1c67f5a446
111 rdf:rest N34c83a05db3d4040bae7df8e5b75ebe0
112 Nf0b67d2805de4b76a79126ee28667832 schema:name dimensions_id
113 schema:value pub.1111979635
114 rdf:type schema:PropertyValue
115 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
116 schema:name Medical and Health Sciences
117 rdf:type schema:DefinedTerm
118 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
119 schema:name Medical Microbiology
120 rdf:type schema:DefinedTerm
121 sg:journal.1045337 schema:issn 2045-2322
122 schema:name Scientific Reports
123 rdf:type schema:Periodical
124 sg:pub.10.1007/s00216-012-5975-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043970511
125 https://doi.org/10.1007/s00216-012-5975-x
126 rdf:type schema:CreativeWork
127 sg:pub.10.1007/s00216-014-7985-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049479107
128 https://doi.org/10.1007/s00216-014-7985-3
129 rdf:type schema:CreativeWork
130 sg:pub.10.1038/nrmicro3432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001049530
131 https://doi.org/10.1038/nrmicro3432
132 rdf:type schema:CreativeWork
133 sg:pub.10.1038/nrmicro841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024064458
134 https://doi.org/10.1038/nrmicro841
135 rdf:type schema:CreativeWork
136 sg:pub.10.1038/srep18570 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044397192
137 https://doi.org/10.1038/srep18570
138 rdf:type schema:CreativeWork
139 sg:pub.10.1038/srep21342 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010148166
140 https://doi.org/10.1038/srep21342
141 rdf:type schema:CreativeWork
142 sg:pub.10.1038/srep38171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046125544
143 https://doi.org/10.1038/srep38171
144 rdf:type schema:CreativeWork
145 https://app.dimensions.ai/details/publication/pub.1078466592 schema:CreativeWork
146 https://app.dimensions.ai/details/publication/pub.1079179552 schema:CreativeWork
147 https://app.dimensions.ai/details/publication/pub.1080029689 schema:CreativeWork
148 https://doi.org/10.1002/anie.200603817 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051066186
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1002/anie.201702776 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085140525
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1016/j.aca.2013.11.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046552458
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1016/j.aca.2018.01.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100810514
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1016/j.bios.2011.04.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019619182
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1016/j.bios.2015.07.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003110384
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1016/j.bios.2016.10.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024840598
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1016/j.bios.2017.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085180171
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1016/j.jchromb.2006.07.054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052384331
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1016/j.juro.2010.09.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048607999
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1016/j.mattod.2016.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012362872
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1016/j.mimet.2010.04.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005547358
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1016/j.mpmed.2007.05.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040066544
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/j.mpmed.2011.04.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025942418
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/j.snb.2013.10.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004286201
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.talanta.2015.07.051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037109824
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.ymeth.2013.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028083053
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/s0140-6736(17)32130-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1091764595
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/s1473-3099(13)70250-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040879612
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1021/ac203466y schema:sameAs https://app.dimensions.ai/details/publication/pub.1030978411
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1021/ac5013249 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055016689
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1021/ac5019205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055019475
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1021/acs.analchem.7b04628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1099725509
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1039/c1lc20764d schema:sameAs https://app.dimensions.ai/details/publication/pub.1053308701
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1039/c2lc21299d schema:sameAs https://app.dimensions.ai/details/publication/pub.1050599888
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1039/c2lc40391a schema:sameAs https://app.dimensions.ai/details/publication/pub.1014976531
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1039/c3lc50887k schema:sameAs https://app.dimensions.ai/details/publication/pub.1026877477
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1039/c5lc01348h schema:sameAs https://app.dimensions.ai/details/publication/pub.1026827333
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1039/c6lc00895j schema:sameAs https://app.dimensions.ai/details/publication/pub.1014972725
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1039/c6lc01450j schema:sameAs https://app.dimensions.ai/details/publication/pub.1083844484
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1039/c8an00632f schema:sameAs https://app.dimensions.ai/details/publication/pub.1104375240
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1080/00365540110080601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015941065
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1080/14737159.2017.1285228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079396356
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1086/383029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020621768
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1086/421997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042899633
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1093/molbev/mst187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012651735
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1128/jcm.44.2.561-570.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041554033
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1177/2211068216680207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034740601
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1371/journal.pone.0018940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049821957
225 rdf:type schema:CreativeWork
226 https://doi.org/10.2471/blt.16.187468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091438015
227 rdf:type schema:CreativeWork
228 https://doi.org/10.3238/arztebl.2010.0361 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078173606
229 rdf:type schema:CreativeWork
230 https://doi.org/10.3390/ijerph7103657 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040035044
231 rdf:type schema:CreativeWork
232 https://doi.org/10.7150/thno.24941 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105066321
233 rdf:type schema:CreativeWork
234 https://www.grid.ac/institutes/grid.7922.e schema:alternateName Chulalongkorn University
235 schema:name Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Patumwan, Bangkok, Thailand
236 Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Chulalongkorn University, 10330, Bangkok, Thailand
237 Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, 10330, Patumwan, Bangkok, Thailand
238 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...