Glatiramer Acetate modulates ion channels expression and calcium homeostasis in B cell of patients with relapsing-remitting multiple sclerosis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

C. Criscuolo, A. Cianflone, R. Lanzillo, D. Carrella, A. Carissimo, F. Napolitano, R. de Cegli, P. de Candia, C. La Rocca, T. Petrozziello, G. Matarese, F. Boscia, A. Secondo, D. Di Bernardo, V. Brescia Morra

ABSTRACT

To investigate the effects of Glatiramer Acetate (GA) on B cells by an integrated computational and experimental approach. GA is an immunomodulatory drug approved for the treatment of multiple sclerosis (MS). GA effect on B cells is yet to be fully elucidated. We compared transcriptional profiles of B cells from treatment-naïve relapsing remitting MS patients, treated or not with GA for 6 hours in vitro, and of B cells before and after six months of GA administration in vivo. Microarrays were analyzed with two different computational approaches, one for functional analysis of pathways (Gene Set Enrichment Analysis) and one for the identification of new drug targets (Mode-of-action by Network Analysis). GA modulates the expression of genes involved in immune response and apoptosis. A differential expression of genes encoding ion channels, mostly regulating Ca2+ homeostasis in endoplasmic reticulum (ER) was also observed. Microfluorimetric analysis confirmed this finding, showing a specific GA effect on ER Ca2+ concentration. Our findings unveils a GA regulatory effect on the immune response by influencing B cell phenotype and function. In particular, our results highlight a new functional role for GA in modulating Ca2+ homeostasis in these cells. More... »

PAGES

4208

References to SciGraph publications

  • 2004-05. Mitoxantrone in CNS DRUGS
  • 2012-07. Ion channels and transporters in lymphocyte function and immunity in NATURE REVIEWS IMMUNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-38152-8

    DOI

    http://dx.doi.org/10.1038/s41598-018-38152-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112706449

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30862866


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Immunology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Department of Neurosciences, Reproductive and Odontostomatological Sciences, \u201cFederico II\u201d University of Naples, 80131, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Criscuolo", 
            "givenName": "C.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "SDN Istituto di Ricerca Diagnostica e Nucleare", 
              "id": "https://www.grid.ac/institutes/grid.482882.c", 
              "name": [
                "IRCCS SDN, Napoli, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cianflone", 
            "givenName": "A.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Department of Neurosciences, Reproductive and Odontostomatological Sciences, \u201cFederico II\u201d University of Naples, 80131, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lanzillo", 
            "givenName": "R.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Telethon Institute Of Genetics And Medicine", 
              "id": "https://www.grid.ac/institutes/grid.410439.b", 
              "name": [
                "Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Carrella", 
            "givenName": "D.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Research Council", 
              "id": "https://www.grid.ac/institutes/grid.5326.2", 
              "name": [
                "Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy", 
                "Institute for Applied Mathematics \u2018Mauro Picone\u2019, National Research Council, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Carissimo", 
            "givenName": "A.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Telethon Institute Of Genetics And Medicine", 
              "id": "https://www.grid.ac/institutes/grid.410439.b", 
              "name": [
                "Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Napolitano", 
            "givenName": "F.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Telethon Institute Of Genetics And Medicine", 
              "id": "https://www.grid.ac/institutes/grid.410439.b", 
              "name": [
                "Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Cegli", 
            "givenName": "R.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MultiMedica", 
              "id": "https://www.grid.ac/institutes/grid.420421.1", 
              "name": [
                "IRCCS MultiMedica, 20138, Milan, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Candia", 
            "givenName": "P.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Research Council", 
              "id": "https://www.grid.ac/institutes/grid.5326.2", 
              "name": [
                "Istituto per l\u2019Endocrinologia e l\u2019Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), 80131, Napoli, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "La Rocca", 
            "givenName": "C.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, \u201cFederico II\u201d University of Naples, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Petrozziello", 
            "givenName": "T.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Istituto per l\u2019Endocrinologia e l\u2019Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), 80131, Napoli, Italy", 
                "Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universit\u00e0 di Napoli Federico II, 80131, Napoli, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Matarese", 
            "givenName": "G.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, \u201cFederico II\u201d University of Naples, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Boscia", 
            "givenName": "F.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, \u201cFederico II\u201d University of Naples, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Secondo", 
            "givenName": "A.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy", 
                "Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, 80125, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Di Bernardo", 
            "givenName": "D.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Naples Federico II", 
              "id": "https://www.grid.ac/institutes/grid.4691.a", 
              "name": [
                "Department of Neurosciences, Reproductive and Odontostomatological Sciences, \u201cFederico II\u201d University of Naples, 80131, Naples, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Morra", 
            "givenName": "V. Brescia", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.3389/fimmu.2015.00642", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000863315"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2478/v10039-009-0045-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003612139"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2478/v10039-009-0045-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003612139"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btg405", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003878496"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btu058", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005220171"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/30.1.207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005297170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.2165/00023210-200418060-00010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006602268", 
              "https://doi.org/10.2165/00023210-200418060-00010"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.2165/00023210-200418060-00010", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006602268", 
              "https://doi.org/10.2165/00023210-200418060-00010"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1586/ern.12.25", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006982839"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.mehy.2004.09.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007110328"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.neuron.2016.02.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009467992"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ejphar.2009.12.027", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010559912"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1132939", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013321903"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev-immunol-032414-112212", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013735131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1352458508095828", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015176126"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1352458508095828", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015176126"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1352458508095828", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015176126"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1001/jamaneurol.2014.1472", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015992473"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m114.555516", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017414476"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m213271200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017728518"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ceca.2007.01.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020120426"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/17.6.509", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024143031"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cels.2015.12.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024709936"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3390/ijms160716576", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029765915"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/glia.22985", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029974905"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nri3233", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031019487", 
              "https://doi.org/10.1038/nri3233"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.20011758", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033010555"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/eji.200838792", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034685238"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2010.06.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036327574"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1001/jamaneurol.2015.3977", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038408127"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.20050687", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042453481"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1084/jem.20050687", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042453481"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.autrev.2016.02.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048332477"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0096256", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048353650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.270.43.25960", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048353943"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/ana.22366", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052372006"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1000138107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053118853"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1001/archneur.61.10.1613", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1054116976"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077821668", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079604630", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.neurobiolaging.2017.03.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084098731"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkx1037", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1092538203"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.celrep.2018.04.053", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103995936"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.celrep.2018.04.053", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103995936"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.celrep.2018.04.053", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1103995936"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-12", 
        "datePublishedReg": "2019-12-01", 
        "description": "To investigate the effects of Glatiramer Acetate (GA) on B cells by an integrated computational and experimental approach. GA is an immunomodulatory drug approved for the treatment of multiple sclerosis (MS). GA effect on B cells is yet to be fully elucidated. We compared transcriptional profiles of B cells from treatment-na\u00efve relapsing remitting MS patients, treated or not with GA for 6\u2009hours in vitro, and of B cells before and after six months of GA administration in vivo. Microarrays were analyzed with two different computational approaches, one for functional analysis of pathways (Gene Set Enrichment Analysis) and one for the identification of new drug targets (Mode-of-action by Network Analysis). GA modulates the expression of genes involved in immune response and apoptosis. A differential expression of genes encoding ion channels, mostly regulating Ca2+ homeostasis in endoplasmic reticulum (ER) was also observed. Microfluorimetric analysis confirmed this finding, showing a specific GA effect on ER Ca2+ concentration. Our findings unveils a GA regulatory effect on the immune response by influencing B cell phenotype and function. In particular, our results highlight a new functional role for GA in modulating Ca2+ homeostasis in these cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/s41598-018-38152-8", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.3798866", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "name": "Glatiramer Acetate modulates ion channels expression and calcium homeostasis in B cell of patients with relapsing-remitting multiple sclerosis", 
        "pagination": "4208", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "cd63ec2f88a0ab6f7bd24dbe2d3f658716e509e5c2e19c602e64c3fe6127e478"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30862866"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101563288"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-018-38152-8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1112706449"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-018-38152-8", 
          "https://app.dimensions.ai/details/publication/pub.1112706449"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:18", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000368_0000000368/records_78938_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/s41598-018-38152-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38152-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38152-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38152-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-38152-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    289 TRIPLES      21 PREDICATES      68 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-018-38152-8 schema:about anzsrc-for:11
    2 anzsrc-for:1107
    3 schema:author Na676de162dd94cc8b47417e1db1e8ca6
    4 schema:citation sg:pub.10.1038/nri3233
    5 sg:pub.10.2165/00023210-200418060-00010
    6 https://app.dimensions.ai/details/publication/pub.1077821668
    7 https://app.dimensions.ai/details/publication/pub.1079604630
    8 https://doi.org/10.1001/archneur.61.10.1613
    9 https://doi.org/10.1001/jamaneurol.2014.1472
    10 https://doi.org/10.1001/jamaneurol.2015.3977
    11 https://doi.org/10.1002/ana.22366
    12 https://doi.org/10.1002/eji.200838792
    13 https://doi.org/10.1002/glia.22985
    14 https://doi.org/10.1016/j.autrev.2016.02.004
    15 https://doi.org/10.1016/j.ceca.2007.01.013
    16 https://doi.org/10.1016/j.cell.2010.06.011
    17 https://doi.org/10.1016/j.celrep.2018.04.053
    18 https://doi.org/10.1016/j.cels.2015.12.004
    19 https://doi.org/10.1016/j.ejphar.2009.12.027
    20 https://doi.org/10.1016/j.mehy.2004.09.024
    21 https://doi.org/10.1016/j.neurobiolaging.2017.03.008
    22 https://doi.org/10.1016/j.neuron.2016.02.022
    23 https://doi.org/10.1073/pnas.0506580102
    24 https://doi.org/10.1073/pnas.1000138107
    25 https://doi.org/10.1074/jbc.270.43.25960
    26 https://doi.org/10.1074/jbc.m114.555516
    27 https://doi.org/10.1074/jbc.m213271200
    28 https://doi.org/10.1084/jem.20011758
    29 https://doi.org/10.1084/jem.20050687
    30 https://doi.org/10.1093/bioinformatics/17.6.509
    31 https://doi.org/10.1093/bioinformatics/btg405
    32 https://doi.org/10.1093/bioinformatics/btu058
    33 https://doi.org/10.1093/nar/30.1.207
    34 https://doi.org/10.1093/nar/gkx1037
    35 https://doi.org/10.1126/science.1132939
    36 https://doi.org/10.1146/annurev-immunol-032414-112212
    37 https://doi.org/10.1177/1352458508095828
    38 https://doi.org/10.1371/journal.pone.0096256
    39 https://doi.org/10.1586/ern.12.25
    40 https://doi.org/10.2478/v10039-009-0045-0
    41 https://doi.org/10.3389/fimmu.2015.00642
    42 https://doi.org/10.3390/ijms160716576
    43 schema:datePublished 2019-12
    44 schema:datePublishedReg 2019-12-01
    45 schema:description To investigate the effects of Glatiramer Acetate (GA) on B cells by an integrated computational and experimental approach. GA is an immunomodulatory drug approved for the treatment of multiple sclerosis (MS). GA effect on B cells is yet to be fully elucidated. We compared transcriptional profiles of B cells from treatment-naïve relapsing remitting MS patients, treated or not with GA for 6 hours in vitro, and of B cells before and after six months of GA administration in vivo. Microarrays were analyzed with two different computational approaches, one for functional analysis of pathways (Gene Set Enrichment Analysis) and one for the identification of new drug targets (Mode-of-action by Network Analysis). GA modulates the expression of genes involved in immune response and apoptosis. A differential expression of genes encoding ion channels, mostly regulating Ca<sup>2+</sup> homeostasis in endoplasmic reticulum (ER) was also observed. Microfluorimetric analysis confirmed this finding, showing a specific GA effect on ER Ca<sup>2+</sup> concentration. Our findings unveils a GA regulatory effect on the immune response by influencing B cell phenotype and function. In particular, our results highlight a new functional role for GA in modulating Ca<sup>2+</sup> homeostasis in these cells.
    46 schema:genre research_article
    47 schema:inLanguage en
    48 schema:isAccessibleForFree true
    49 schema:isPartOf Na428c5c5bf914ca591576c9ec318c803
    50 Nb1b38edceb344ddaa54367df26a7f281
    51 sg:journal.1045337
    52 schema:name Glatiramer Acetate modulates ion channels expression and calcium homeostasis in B cell of patients with relapsing-remitting multiple sclerosis
    53 schema:pagination 4208
    54 schema:productId N14cef99de0034dc3a0650fd53ea85bd4
    55 N3e92c23bbf504356a90117060e7228ed
    56 N472fbdf547a84ae2a244e707c730f00b
    57 N7ea2c78999534c09bcdecefa71b949ac
    58 Nd0c927b1c5154acfa75de97c228b8e05
    59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112706449
    60 https://doi.org/10.1038/s41598-018-38152-8
    61 schema:sdDatePublished 2019-04-11T13:18
    62 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    63 schema:sdPublisher N5e518cff4456495fa3a77678aab24798
    64 schema:url https://www.nature.com/articles/s41598-018-38152-8
    65 sgo:license sg:explorer/license/
    66 sgo:sdDataset articles
    67 rdf:type schema:ScholarlyArticle
    68 N01746537c9cc4fd68f078527f90d2a0c rdf:first Ndd301cd342e54a7c84ce3be9b94688af
    69 rdf:rest N51cfa382e2dc4eb6957b366cbeab21ba
    70 N114eddef412341af8b21458d9d26d292 schema:affiliation https://www.grid.ac/institutes/grid.410439.b
    71 schema:familyName Carrella
    72 schema:givenName D.
    73 rdf:type schema:Person
    74 N14cef99de0034dc3a0650fd53ea85bd4 schema:name readcube_id
    75 schema:value cd63ec2f88a0ab6f7bd24dbe2d3f658716e509e5c2e19c602e64c3fe6127e478
    76 rdf:type schema:PropertyValue
    77 N22270e200dbb465dbd94f1013968c1bd schema:affiliation https://www.grid.ac/institutes/grid.5326.2
    78 schema:familyName Carissimo
    79 schema:givenName A.
    80 rdf:type schema:Person
    81 N3126cb2838f94783930211cbb262ed81 rdf:first N114eddef412341af8b21458d9d26d292
    82 rdf:rest Nd18f793934bb4112b2dba465be9d1023
    83 N31d0f0db694b4922a1c3f022d8c4f840 rdf:first Ne12c75f91826413b91310a656549d694
    84 rdf:rest Ncc5c8bbbad5e454e81a2ccaa9a19f2bf
    85 N3e92c23bbf504356a90117060e7228ed schema:name dimensions_id
    86 schema:value pub.1112706449
    87 rdf:type schema:PropertyValue
    88 N472fbdf547a84ae2a244e707c730f00b schema:name doi
    89 schema:value 10.1038/s41598-018-38152-8
    90 rdf:type schema:PropertyValue
    91 N4c01116085b542c3894e196651faf422 rdf:first Ncd1bb81db0b04168a2ebd2f3b2c4c393
    92 rdf:rest Na0522291c90247b082f152a9b75b88ec
    93 N51cfa382e2dc4eb6957b366cbeab21ba rdf:first N8d9d65c1197a46d8a46078192818b83a
    94 rdf:rest Na96d745e8de74d6d90d52cc15c8fba49
    95 N53060d1836ce4dd0b35db6b229da458f rdf:first N7867104e30df412b9f0e53e2576a7601
    96 rdf:rest N5da088ef5f1c4508b96ab6a7537b808b
    97 N5da088ef5f1c4508b96ab6a7537b808b rdf:first Nb6015e1091874c4da76a0ad044f78624
    98 rdf:rest Nc92bf07b00744ecdb0d68cce8e84dd22
    99 N5e518cff4456495fa3a77678aab24798 schema:name Springer Nature - SN SciGraph project
    100 rdf:type schema:Organization
    101 N7867104e30df412b9f0e53e2576a7601 schema:affiliation https://www.grid.ac/institutes/grid.410439.b
    102 schema:familyName Napolitano
    103 schema:givenName F.
    104 rdf:type schema:Person
    105 N7ea2c78999534c09bcdecefa71b949ac schema:name pubmed_id
    106 schema:value 30862866
    107 rdf:type schema:PropertyValue
    108 N8108b715bd4147ae9beef64e69f7df42 rdf:first Ndf0ae6ad09a146d2a7dc728e0762d84c
    109 rdf:rest N9e91323d1dd249aaac1858b0f1065e1e
    110 N8d9d65c1197a46d8a46078192818b83a schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    111 schema:familyName Petrozziello
    112 schema:givenName T.
    113 rdf:type schema:Person
    114 N986eae4b9c394c7881016b03f27fea53 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    115 schema:familyName Lanzillo
    116 schema:givenName R.
    117 rdf:type schema:Person
    118 N9e91323d1dd249aaac1858b0f1065e1e rdf:first Nc341e2e2156a4573a0e399060237f9ff
    119 rdf:rest rdf:nil
    120 Na0522291c90247b082f152a9b75b88ec rdf:first N986eae4b9c394c7881016b03f27fea53
    121 rdf:rest N3126cb2838f94783930211cbb262ed81
    122 Na428c5c5bf914ca591576c9ec318c803 schema:volumeNumber 9
    123 rdf:type schema:PublicationVolume
    124 Na676de162dd94cc8b47417e1db1e8ca6 rdf:first Nca0515f6e69043bd8b05d7384ef2cd1f
    125 rdf:rest N4c01116085b542c3894e196651faf422
    126 Na70e4a5428ba40b0b13bc5faca9fe0e6 schema:affiliation https://www.grid.ac/institutes/grid.420421.1
    127 schema:familyName de Candia
    128 schema:givenName P.
    129 rdf:type schema:Person
    130 Na96d745e8de74d6d90d52cc15c8fba49 rdf:first Nc6f7f8d1b56144deaf80505daeddfcb8
    131 rdf:rest N31d0f0db694b4922a1c3f022d8c4f840
    132 Naa4c6a933f7f40deb701c82a9da2ee93 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    133 schema:familyName Secondo
    134 schema:givenName A.
    135 rdf:type schema:Person
    136 Nb1b38edceb344ddaa54367df26a7f281 schema:issueNumber 1
    137 rdf:type schema:PublicationIssue
    138 Nb6015e1091874c4da76a0ad044f78624 schema:affiliation https://www.grid.ac/institutes/grid.410439.b
    139 schema:familyName de Cegli
    140 schema:givenName R.
    141 rdf:type schema:Person
    142 Nc341e2e2156a4573a0e399060237f9ff schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    143 schema:familyName Morra
    144 schema:givenName V. Brescia
    145 rdf:type schema:Person
    146 Nc6f7f8d1b56144deaf80505daeddfcb8 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    147 schema:familyName Matarese
    148 schema:givenName G.
    149 rdf:type schema:Person
    150 Nc92bf07b00744ecdb0d68cce8e84dd22 rdf:first Na70e4a5428ba40b0b13bc5faca9fe0e6
    151 rdf:rest N01746537c9cc4fd68f078527f90d2a0c
    152 Nca0515f6e69043bd8b05d7384ef2cd1f schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    153 schema:familyName Criscuolo
    154 schema:givenName C.
    155 rdf:type schema:Person
    156 Ncc5c8bbbad5e454e81a2ccaa9a19f2bf rdf:first Naa4c6a933f7f40deb701c82a9da2ee93
    157 rdf:rest N8108b715bd4147ae9beef64e69f7df42
    158 Ncd1bb81db0b04168a2ebd2f3b2c4c393 schema:affiliation https://www.grid.ac/institutes/grid.482882.c
    159 schema:familyName Cianflone
    160 schema:givenName A.
    161 rdf:type schema:Person
    162 Nd0c927b1c5154acfa75de97c228b8e05 schema:name nlm_unique_id
    163 schema:value 101563288
    164 rdf:type schema:PropertyValue
    165 Nd18f793934bb4112b2dba465be9d1023 rdf:first N22270e200dbb465dbd94f1013968c1bd
    166 rdf:rest N53060d1836ce4dd0b35db6b229da458f
    167 Ndd301cd342e54a7c84ce3be9b94688af schema:affiliation https://www.grid.ac/institutes/grid.5326.2
    168 schema:familyName La Rocca
    169 schema:givenName C.
    170 rdf:type schema:Person
    171 Ndf0ae6ad09a146d2a7dc728e0762d84c schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    172 schema:familyName Di Bernardo
    173 schema:givenName D.
    174 rdf:type schema:Person
    175 Ne12c75f91826413b91310a656549d694 schema:affiliation https://www.grid.ac/institutes/grid.4691.a
    176 schema:familyName Boscia
    177 schema:givenName F.
    178 rdf:type schema:Person
    179 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    180 schema:name Medical and Health Sciences
    181 rdf:type schema:DefinedTerm
    182 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
    183 schema:name Immunology
    184 rdf:type schema:DefinedTerm
    185 sg:grant.3798866 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-018-38152-8
    186 rdf:type schema:MonetaryGrant
    187 sg:journal.1045337 schema:issn 2045-2322
    188 schema:name Scientific Reports
    189 rdf:type schema:Periodical
    190 sg:pub.10.1038/nri3233 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031019487
    191 https://doi.org/10.1038/nri3233
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.2165/00023210-200418060-00010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006602268
    194 https://doi.org/10.2165/00023210-200418060-00010
    195 rdf:type schema:CreativeWork
    196 https://app.dimensions.ai/details/publication/pub.1077821668 schema:CreativeWork
    197 https://app.dimensions.ai/details/publication/pub.1079604630 schema:CreativeWork
    198 https://doi.org/10.1001/archneur.61.10.1613 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054116976
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1001/jamaneurol.2014.1472 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015992473
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1001/jamaneurol.2015.3977 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038408127
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1002/ana.22366 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052372006
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1002/eji.200838792 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034685238
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1002/glia.22985 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029974905
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1016/j.autrev.2016.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048332477
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1016/j.ceca.2007.01.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020120426
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/j.cell.2010.06.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036327574
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1016/j.celrep.2018.04.053 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103995936
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1016/j.cels.2015.12.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024709936
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1016/j.ejphar.2009.12.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010559912
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1016/j.mehy.2004.09.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007110328
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1016/j.neurobiolaging.2017.03.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084098731
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1016/j.neuron.2016.02.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009467992
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1073/pnas.0506580102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037705714
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1073/pnas.1000138107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053118853
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1074/jbc.270.43.25960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048353943
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1074/jbc.m114.555516 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017414476
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1074/jbc.m213271200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017728518
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1084/jem.20011758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033010555
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1084/jem.20050687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042453481
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1093/bioinformatics/17.6.509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024143031
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1093/bioinformatics/btg405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003878496
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1093/bioinformatics/btu058 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005220171
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1093/nar/30.1.207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005297170
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1093/nar/gkx1037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092538203
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1126/science.1132939 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013321903
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1146/annurev-immunol-032414-112212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013735131
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1177/1352458508095828 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015176126
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1371/journal.pone.0096256 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048353650
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1586/ern.12.25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006982839
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.2478/v10039-009-0045-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003612139
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.3389/fimmu.2015.00642 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000863315
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.3390/ijms160716576 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029765915
    267 rdf:type schema:CreativeWork
    268 https://www.grid.ac/institutes/grid.410439.b schema:alternateName Telethon Institute Of Genetics And Medicine
    269 schema:name Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy
    270 rdf:type schema:Organization
    271 https://www.grid.ac/institutes/grid.420421.1 schema:alternateName MultiMedica
    272 schema:name IRCCS MultiMedica, 20138, Milan, Italy
    273 rdf:type schema:Organization
    274 https://www.grid.ac/institutes/grid.4691.a schema:alternateName University of Naples Federico II
    275 schema:name Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, 80125, Naples, Italy
    276 Department of Neurosciences, Reproductive and Odontostomatological Sciences, “Federico II” University of Naples, 80131, Naples, Italy
    277 Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, 80131, Napoli, Italy
    278 Division of Pharmacology, Department of Neuroscience, Reproductive and Odontostomatological Sciences, School of Medicine, “Federico II” University of Naples, Naples, Italy
    279 Istituto per l’Endocrinologia e l’Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), 80131, Napoli, Italy
    280 Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy
    281 rdf:type schema:Organization
    282 https://www.grid.ac/institutes/grid.482882.c schema:alternateName SDN Istituto di Ricerca Diagnostica e Nucleare
    283 schema:name IRCCS SDN, Napoli, Italy
    284 rdf:type schema:Organization
    285 https://www.grid.ac/institutes/grid.5326.2 schema:alternateName National Research Council
    286 schema:name Institute for Applied Mathematics ‘Mauro Picone’, National Research Council, Naples, Italy
    287 Istituto per l’Endocrinologia e l’Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), 80131, Napoli, Italy
    288 Telethon Institute of Genetics and Medicine (TIGEM), 80078, Pozzuoli, (NA), Italy
    289 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...