Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks View Full Text


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Article Info

DATE

2019-02-06

AUTHORS

Valentina Mastrantonio, Maria Stefania Latrofa, Daniele Porretta, Riccardo Paolo Lia, Antonio Parisi, Roberta Iatta, Filipe Dantas-Torres, Domenico Otranto, Sandra Urbanelli

ABSTRACT

Paternal leakage of mitochondrial DNA (mtDNA) and heteroplasmy have been recently described in several animal species. In arthropods, by searching in the Scopus database, we found only 23 documented cases of paternal leakage. Therefore, although arthropods represent a large fraction of animal biodiversity, this phenomenon has been investigated only in a paucity of species in this phylum, thus preventing a reliable estimate of its frequency. Here, we investigated the occurrence of paternal leakage and mtDNA heteroplasmy in ticks belonging to one of the most significant tick species complexes, the so-called Rhipicephalus sanguineussensu lato. By developing a multiplex allele-specific PCR assay targeting a fragment of the 12S rRNA ribosomal region of the mtDNA, we showed the occurrence of paternal leakage and mtDNA heteroplasmy in R. sanguineuss.l. ticks originated from experimental crosses, as well as in individuals collected from the field. Our results add a new evidence of paternal leakage in arthropods and document for the first time this phenomenon in ticks. Furthermore, they suggest the importance of using allele-specific assays when searching for paternal leakage and/or heteroplasmy, as standard sequencing methods may fail to detect the rare mtDNA molecules. More... »

PAGES

1460

References to SciGraph publications

  • 2014-12-11. Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi in SCIENTIFIC REPORTS
  • 2018-07-09. Biological compatibility between two temperate lineages of brown dog ticks, Rhipicephalus sanguineus (sensu lato) in PARASITES & VECTORS
  • 2016-07-27. Dynamics of mtDNA introgression during species range expansion: insights from an experimental longitudinal study in SCIENTIFIC REPORTS
  • 2016-12-12. Exon-intron structure and sequence variation of the calreticulin gene among Rhipicephalus sanguineus group ticks in PARASITES & VECTORS
  • 1993-12. Transfer of paternal mitochondrial DNA during fertilization of honeybee (Apis mellifera L.) eggs in CURRENT GENETICS
  • 2017-09-13. Admixture on the northern front: population genomics of range expansion in the white-footed mouse (Peromyscus leucopus) and secondary contact with the deer mouse (Peromyscus maniculatus) in HEREDITY
  • 2013-05-17. DNA abandonment and the mechanisms of uniparental inheritance of mitochondria and chloroplasts in CHROMOSOME RESEARCH
  • 2017-01-23. East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin in PARASITES & VECTORS
  • 2013-07-23. Morphological and genetic diversity of Rhipicephalus sanguineus sensu lato from the New and Old Worlds in PARASITES & VECTORS
  • <error retrieving object. in <ERROR RETRIEVING OBJECT
  • 2017-01-31. Evolution and inheritance of animal mitochondrial DNA: rules and exceptions in JOURNAL OF BIOLOGICAL RESEARCH-THESSALONIKI
  • 2016-01-04. Abundance and seasonal activity of Haemaphysalis concinna (Acari: Ixodidae) at the border between China and Russia in Northern Inner Mongolia, China in PARASITES & VECTORS
  • 2018-09-04. Hybridization and extensive mitochondrial introgression among fire salamanders in peninsular Italy in SCIENTIFIC REPORTS
  • 2007-09-29. Structure and Evolution of the Atypical Mitochondrial Genome of Armadillidium vulgare (Isopoda, Crustacea) in JOURNAL OF MOLECULAR EVOLUTION
  • 2017-03-13. Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal in PARASITES & VECTORS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-38001-8

    DOI

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    31 schema:description Paternal leakage of mitochondrial DNA (mtDNA) and heteroplasmy have been recently described in several animal species. In arthropods, by searching in the Scopus database, we found only 23 documented cases of paternal leakage. Therefore, although arthropods represent a large fraction of animal biodiversity, this phenomenon has been investigated only in a paucity of species in this phylum, thus preventing a reliable estimate of its frequency. Here, we investigated the occurrence of paternal leakage and mtDNA heteroplasmy in ticks belonging to one of the most significant tick species complexes, the so-called Rhipicephalus sanguineussensu lato. By developing a multiplex allele-specific PCR assay targeting a fragment of the 12S rRNA ribosomal region of the mtDNA, we showed the occurrence of paternal leakage and mtDNA heteroplasmy in R. sanguineuss.l. ticks originated from experimental crosses, as well as in individuals collected from the field. Our results add a new evidence of paternal leakage in arthropods and document for the first time this phenomenon in ticks. Furthermore, they suggest the importance of using allele-specific assays when searching for paternal leakage and/or heteroplasmy, as standard sequencing methods may fail to detect the rare mtDNA molecules.
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    38 PCR
    39 R. sanguineus
    40 Rhipicephalus spp
    41 Scopus databases
    42 allele-specific PCR
    43 allele-specific assays
    44 animal biodiversity
    45 animal species
    46 arthropods
    47 assays
    48 biodiversity
    49 cases
    50 complexes
    51 cross
    52 database
    53 documents
    54 estimates
    55 evidence
    56 experimental crosses
    57 field
    58 first time
    59 fraction
    60 fragments
    61 frequency
    62 heteroplasmy
    63 importance
    64 individuals
    65 large fraction
    66 lato
    67 leakage
    68 method
    69 mitochondrial DNA
    70 molecules
    71 mtDNA
    72 mtDNA heteroplasmy
    73 mtDNA molecules
    74 multiplex allele-specific PCR
    75 new evidence
    76 occurrence
    77 paternal leakage
    78 paucity
    79 paucity of species
    80 phenomenon
    81 phyla
    82 region
    83 reliable estimates
    84 results
    85 ribosomal region
    86 sanguineus
    87 sequencing methods
    88 species
    89 species complex
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