Cu Transport by the Extended Family of CcoA-like Transporters (CalT) in Proteobacteria View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Yang Zhang, Crysten E. Blaby-Haas, Stefan Steimle, Andreia F. Verissimo, Victor A. Garcia-Angulo, Hans-Georg Koch, Fevzi Daldal, Bahia Khalfaoui-Hassani

ABSTRACT

Comparative genomic studies of the bacterial MFS-type copper importer CcoA, required for cbb3-type cytochrome c oxidase (cbb3-Cox) biogenesis, revealed a widespread CcoA-like transporters (CalT) family, containing the conserved CcoA Cu-binding MxxxM and HxxxM motifs. Surprisingly, this family also included the RfnT-like proteins, earlier suggested to transport riboflavin. However, presence of the Cu-binding motifs in these proteins raised the possibility that they might be Cu transporters. To test this hypothesis, the genomic context of the corresponding genes was examined, and three of such genes from Ochrobactrum anthropi, Rhodopseudomonas palustris and Agrobacterium tumefaciens were expressed in Escherichia coli (ΔribB) and Rhodobacter capsulatus (ΔccoA) mutants. Copper and riboflavin uptake abilities of these strains were compared with those expressing R. capsulatus CcoA and Rhizobium leguminosarum RibN as bona fide copper and riboflavin importers, respectively. Overall data demonstrated that the "RfnT-like" CalT proteins are unable to efficiently transport riboflavin, but they import copper like CcoA. Nevertheless, even though expressed and membrane-localized in a R. capsulatus mutant lacking CcoA, these transporters were unable to accumulate Cu or complement for cbb3-Cox defect. This lack of functional exchangeability between the different subfamilies of CalT homologs suggests that MFS-type bacterial copper importers might be species-specific. More... »

PAGES

1208

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-37988-4

DOI

http://dx.doi.org/10.1038/s41598-018-37988-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111919287

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30718766


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37988-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37988-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37988-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37988-4'


 

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296 https://www.grid.ac/institutes/grid.462187.e schema:alternateName Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux
297 schema:name Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA
298 IPREM, UMR CNRS 5254, and Université de Pau et des Pays de l’Adour, BP1155, Pau, France
299 rdf:type schema:Organization
300 https://www.grid.ac/institutes/grid.5963.9 schema:alternateName University of Freiburg
301 schema:name Institut für Biochemie und Molekularbiologie, ZBMZ, Faculty of Medicine, Stefan-Meier-Strasse 17, Albert-Ludwigs-Universität Freiburg, 79104, Freiburg, Germany
302 rdf:type schema:Organization
 




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