A Surface Plasmon Resonance-based assay to measure serum concentrations of therapeutic antibodies and anti-drug antibodies View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Marten Beeg, Alessandro Nobili, Barbara Orsini, Francesca Rogai, Daniela Gilardi, Gionata Fiorino, Silvio Danese, Mario Salmona, Silvio Garattini, Marco Gobbi

ABSTRACT

Therapeutic drug and immunogenicity monitoring (TDIM) is increasingly proposed to guide therapy with biologics, characterised by high inter-individual variability of their blood levels, to permit objective decisions for the management of non-responders and reduce unnecessary interventions with these expensive treatments. However, TDIM has not yet entered clinical practice partly because of uncertainties regarding the accuracy and precision of enzyme-linked immunosorbent assays (ELISA). Here we report the characterisation of a novel surface plasmon resonance (SPR)-based TDIM, applied to the measurement of serum concentrations of infliximab, an antibody against tumour necrosis factor α (anti-TNFα), and anti-infliximab antibodies. SPR has the obvious advantages of directly detecting and measuring serum antibodies in minutes, avoiding the long incubation/separation/washing/detection steps of the methods proposed so far, reducing complexity and variability. Moreover, drug and anti-drug antibodies can be measured simultaneously. This new method was validated for sensitivity and reproducibility, and showed cost-effectiveness over commercial ELISA kits. This method may be applied to other biotherapeutics. These data pave the way for the development of SPR-based point-of-care devices for rapid on-site analysis. More... »

PAGES

2064

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-37950-4

    DOI

    http://dx.doi.org/10.1038/s41598-018-37950-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112133958

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30765716


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