Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

L. G. V. Fernandes, L. P. Guaman, S. A. Vasconcellos, Marcos B. Heinemann, M. Picardeau, A. L. T. O. Nascimento

ABSTRACT

Leptospirosis is a worldwide zoonosis caused by pathogenic bacteria of the genus Leptospira, which also includes free-living saprophyte strains. Many aspects of leptospiral basic biology and virulence mechanisms remain unexplored mainly due to the lack of effective genetic tools available for these bacteria. Recently, the type II CRISPR/Cas system from Streptococcus pyogenes has been widely used as an efficient genome engineering tool in bacteria by inducing double-strand breaks (DSBs) in the desired genomic targets caused by an RNA-guided DNA endonuclease called Cas9, and the DSB repair associated machinery. In the present work, plasmids expressing heterologous S. pyogenes Cas9 in L. biflexa cells were generated, and the enzyme could be expressed with no apparent toxicity to leptospiral cells. However, L. biflexa cells were unable to repair RNA-guided Cas9-induced DSBs. Thus, we used a catalytically dead Cas9 (dCas9) to obtain gene silencing rather than disruption, in a strategy called CRISPR interference (CRISPRi). We demonstrated complete gene silencing in L. biflexa cells when both dCas9 and single-guide RNA (sgRNA) targeting the coding strand of the β-galactosidase gene were expressed simultaneously. Furthermore, when the system was applied for silencing the dnaK gene, no colonies were recovered, indicating that DnaK protein is essential in Leptospira. In addition, flagellar motor switch FliG gene silencing resulted in reduced bacterial motility. To the best of our knowledge, this is the first work applying the CRISPRi system in Leptospira and spirochetes in general, expanding the tools available for understanding leptospiral biology. More... »

PAGES

1839

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-37949-x

DOI

http://dx.doi.org/10.1038/s41598-018-37949-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112063978

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30755626


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Instituto Butantan", 
          "id": "https://www.grid.ac/institutes/grid.418514.d", 
          "name": [
            "Laborat\u00f3rio Especial de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503-900, Sao Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fernandes", 
        "givenName": "L. G. V.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universidad Tecnol\u00f3gica Equinoccial", 
          "id": "https://www.grid.ac/institutes/grid.412257.7", 
          "name": [
            "Universidad Tecnol\u00f3gica Equinoccial, Centro de Investigaci\u00f3n Biom\u00e9dica, Facultad de Ciencias de la Salud Eugenio Espejo, Avenida Mariscal Sucre y Mariana de Jes\u00fas. Campus Occidental, 170105, Quito, Ecuador"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Guaman", 
        "givenName": "L. P.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508-270, Sao Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vasconcellos", 
        "givenName": "S. A.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Sao Paulo", 
          "id": "https://www.grid.ac/institutes/grid.11899.38", 
          "name": [
            "Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508-270, Sao Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heinemann", 
        "givenName": "Marcos B.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut Pasteur", 
          "id": "https://www.grid.ac/institutes/grid.428999.7", 
          "name": [
            "Institut Pasteur, Biology of Spirochetes Unit, 25 rue du Dr Roux, 75723, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Picardeau", 
        "givenName": "M.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto Butantan", 
          "id": "https://www.grid.ac/institutes/grid.418514.d", 
          "name": [
            "Laborat\u00f3rio Especial de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503-900, Sao Paulo, SP, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nascimento", 
        "givenName": "A. L. T. O.", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1128/iai.00131-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000416889"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkt520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002402497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/sb500351f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003383831"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.02172-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003614845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/9780471729259.mc12e04s05", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005174790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2010.07078.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005685461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2010.07078.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005685461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.3011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007861343", 
          "https://doi.org/10.1038/nbt.3011"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/cr.2013.146", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007939040", 
          "https://doi.org/10.1038/cr.2013.146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2013.02.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008291683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2016.05.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008876849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09523", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009515358", 
          "https://doi.org/10.1038/nature09523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09523", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009515358", 
          "https://doi.org/10.1038/nature09523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/427110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009670564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/427110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009670564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.00989-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009682018"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0136690", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010259449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1631908", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011253653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms7267", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014345430", 
          "https://doi.org/10.1038/ncomms7267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s40484-014-0030-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014529473", 
          "https://doi.org/10.1007/s40484-014-0030-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00173-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016224700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.01546-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016289015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.182.20.5700-5705.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017485070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2012.07985.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018329845"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1473-3099(03)00830-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018864760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gku410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020146143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.02042-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020478365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1232033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022072971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.052308.093131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022097335"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-61779-603-6_9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022263055", 
          "https://doi.org/10.1007/978-1-61779-603-6_9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chom.2012.06.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025013559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkw223", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025545721"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.dnarep.2004.06.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029232834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0035-9203(70)90087-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029551515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029773752", 
          "https://doi.org/10.1038/nsmb915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb915", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029773752", 
          "https://doi.org/10.1038/nsmb915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkw231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030550273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.01293-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030914414"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibtech.2013.04.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031527623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.resmic.2004.09.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032407606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00335-015-9565-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032581219", 
          "https://doi.org/10.1007/s00335-015-9565-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00335-015-9565-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032581219", 
          "https://doi.org/10.1007/s00335-015-9565-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.2009.01542.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034029086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.qco.0000178824.05715.2c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034132415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.qco.0000178824.05715.2c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034132415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.qco.0000178824.05715.2c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034132415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.15.4.1968", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034391289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.181001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034860782"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.04023-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035109565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pntd.0003952", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035650849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2001.02374.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035997180"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1138140", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036312168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gku241", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038476578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mbio.00928-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039604015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.2650", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040481921", 
          "https://doi.org/10.1038/nbt.2650"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.02202-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040489672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1590/s0100-879x2004000400003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040970778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1225829", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041850060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkt135", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042813151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.186.7.2164-2172.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044705615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2006.10.046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045885195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.0030097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046412884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fcimb.2017.00010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049896955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00873-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051464659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkw625", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051483332"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2010.07360.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051916975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2010.07360.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051916975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2009.03.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053273451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.01619-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053281388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/ijs.0.047324-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060385131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079196676", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079425218", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00291-17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084852307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00291-17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084852307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fcimb.2017.00214", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085599291"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12934-017-0802-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092515990", 
          "https://doi.org/10.1186/s12934-017-0802-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m117.804443", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092730856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pgen.1007148", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100085807"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00575-17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1100257882"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41579-018-0002-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101821595", 
          "https://doi.org/10.1038/s41579-018-0002-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/s41579-018-0002-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1101821595", 
          "https://doi.org/10.1038/s41579-018-0002-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-018-8927-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103183290", 
          "https://doi.org/10.1007/s00253-018-8927-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-018-8927-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103183290", 
          "https://doi.org/10.1007/s00253-018-8927-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-018-8927-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103183290", 
          "https://doi.org/10.1007/s00253-018-8927-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0194923", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103202960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fcimb.2018.00130", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103857347"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/biot.201800121", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104374989"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Leptospirosis is a worldwide zoonosis caused by pathogenic bacteria of the genus Leptospira, which also includes free-living saprophyte strains. Many aspects of leptospiral basic biology and virulence mechanisms remain unexplored mainly due to the lack of effective genetic tools available for these bacteria. Recently, the type II CRISPR/Cas system from Streptococcus pyogenes has been widely used as an efficient genome engineering tool in bacteria by inducing double-strand breaks (DSBs) in the desired genomic targets caused by an RNA-guided DNA endonuclease called Cas9, and the DSB repair associated machinery. In the present work, plasmids expressing heterologous S. pyogenes Cas9 in L. biflexa cells were generated, and the enzyme could be expressed with no apparent toxicity to leptospiral cells. However, L. biflexa cells were unable to repair RNA-guided Cas9-induced DSBs. Thus, we used a catalytically dead Cas9 (dCas9) to obtain gene silencing rather than disruption, in a strategy called CRISPR interference (CRISPRi). We demonstrated complete gene silencing in L. biflexa cells when both dCas9 and single-guide RNA (sgRNA) targeting the coding strand of the \u03b2-galactosidase gene were expressed simultaneously. Furthermore, when the system was applied for silencing the dnaK gene, no colonies were recovered, indicating that DnaK protein is essential in Leptospira. In addition, flagellar motor switch FliG gene silencing resulted in reduced bacterial motility. To the best of our knowledge, this is the first work applying the CRISPRi system in Leptospira and spirochetes in general, expanding the tools available for understanding leptospiral biology.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-37949-x", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4604028", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.4543480", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira", 
    "pagination": "1839", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1e332f4746348ffdca06f1a5d7797434779f688baba55052df2f1954ef85d8b9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30755626"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-37949-x"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112063978"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-37949-x", 
      "https://app.dimensions.ai/details/publication/pub.1112063978"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:09", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000338_0000000338/records_47966_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-37949-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37949-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37949-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37949-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37949-x'


 

This table displays all metadata directly associated to this object as RDF triples.

345 TRIPLES      21 PREDICATES      104 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-37949-x schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Nf877960a4f9e4652aa5fdd46c1afeb25
4 schema:citation sg:pub.10.1007/978-1-61779-603-6_9
5 sg:pub.10.1007/s00253-018-8927-3
6 sg:pub.10.1007/s00335-015-9565-z
7 sg:pub.10.1007/s40484-014-0030-x
8 sg:pub.10.1038/cr.2013.146
9 sg:pub.10.1038/nature09523
10 sg:pub.10.1038/nbt.2650
11 sg:pub.10.1038/nbt.3011
12 sg:pub.10.1038/ncomms7267
13 sg:pub.10.1038/nsmb915
14 sg:pub.10.1038/s41579-018-0002-7
15 sg:pub.10.1186/s12934-017-0802-x
16 https://app.dimensions.ai/details/publication/pub.1079196676
17 https://app.dimensions.ai/details/publication/pub.1079425218
18 https://doi.org/10.1002/9780471729259.mc12e04s05
19 https://doi.org/10.1002/biot.201800121
20 https://doi.org/10.1016/0035-9203(70)90087-8
21 https://doi.org/10.1016/j.cell.2013.02.022
22 https://doi.org/10.1016/j.cell.2016.05.003
23 https://doi.org/10.1016/j.chom.2012.06.003
24 https://doi.org/10.1016/j.dnarep.2004.06.003
25 https://doi.org/10.1016/j.jmb.2006.10.046
26 https://doi.org/10.1016/j.resmic.2004.09.007
27 https://doi.org/10.1016/j.tibtech.2013.04.004
28 https://doi.org/10.1016/j.vetmic.2009.03.012
29 https://doi.org/10.1016/s1473-3099(03)00830-2
30 https://doi.org/10.1021/sb500351f
31 https://doi.org/10.1046/j.1365-2958.2001.02374.x
32 https://doi.org/10.1074/jbc.m117.804443
33 https://doi.org/10.1086/427110
34 https://doi.org/10.1093/nar/gkt135
35 https://doi.org/10.1093/nar/gkt520
36 https://doi.org/10.1093/nar/gku241
37 https://doi.org/10.1093/nar/gku410
38 https://doi.org/10.1093/nar/gkw223
39 https://doi.org/10.1093/nar/gkw231
40 https://doi.org/10.1093/nar/gkw625
41 https://doi.org/10.1097/01.qco.0000178824.05715.2c
42 https://doi.org/10.1099/ijs.0.047324-0
43 https://doi.org/10.1101/gad.1631908
44 https://doi.org/10.1101/gr.181001
45 https://doi.org/10.1111/j.1365-2958.2010.07078.x
46 https://doi.org/10.1111/j.1365-2958.2010.07360.x
47 https://doi.org/10.1111/j.1365-2958.2012.07985.x
48 https://doi.org/10.1111/j.1574-6968.2009.01542.x
49 https://doi.org/10.1126/science.1138140
50 https://doi.org/10.1126/science.1225829
51 https://doi.org/10.1126/science.1232033
52 https://doi.org/10.1128/aem.00173-15
53 https://doi.org/10.1128/aem.00291-17
54 https://doi.org/10.1128/aem.00873-15
55 https://doi.org/10.1128/aem.01619-14
56 https://doi.org/10.1128/aem.02172-07
57 https://doi.org/10.1128/aem.02202-15
58 https://doi.org/10.1128/aem.04023-14
59 https://doi.org/10.1128/iai.00131-12
60 https://doi.org/10.1128/iai.00989-08
61 https://doi.org/10.1128/iai.01293-08
62 https://doi.org/10.1128/iai.01546-13
63 https://doi.org/10.1128/jb.00575-17
64 https://doi.org/10.1128/jb.02042-14
65 https://doi.org/10.1128/jb.182.20.5700-5705.2000
66 https://doi.org/10.1128/jb.186.7.2164-2172.2004
67 https://doi.org/10.1128/mbio.00928-13
68 https://doi.org/10.1128/mcb.15.4.1968
69 https://doi.org/10.1146/annurev.biochem.052308.093131
70 https://doi.org/10.1371/journal.pgen.1007148
71 https://doi.org/10.1371/journal.pntd.0003952
72 https://doi.org/10.1371/journal.pone.0136690
73 https://doi.org/10.1371/journal.pone.0194923
74 https://doi.org/10.1371/journal.ppat.0030097
75 https://doi.org/10.1590/s0100-879x2004000400003
76 https://doi.org/10.3389/fcimb.2017.00010
77 https://doi.org/10.3389/fcimb.2017.00214
78 https://doi.org/10.3389/fcimb.2018.00130
79 schema:datePublished 2019-12
80 schema:datePublishedReg 2019-12-01
81 schema:description Leptospirosis is a worldwide zoonosis caused by pathogenic bacteria of the genus Leptospira, which also includes free-living saprophyte strains. Many aspects of leptospiral basic biology and virulence mechanisms remain unexplored mainly due to the lack of effective genetic tools available for these bacteria. Recently, the type II CRISPR/Cas system from Streptococcus pyogenes has been widely used as an efficient genome engineering tool in bacteria by inducing double-strand breaks (DSBs) in the desired genomic targets caused by an RNA-guided DNA endonuclease called Cas9, and the DSB repair associated machinery. In the present work, plasmids expressing heterologous S. pyogenes Cas9 in L. biflexa cells were generated, and the enzyme could be expressed with no apparent toxicity to leptospiral cells. However, L. biflexa cells were unable to repair RNA-guided Cas9-induced DSBs. Thus, we used a catalytically dead Cas9 (dCas9) to obtain gene silencing rather than disruption, in a strategy called CRISPR interference (CRISPRi). We demonstrated complete gene silencing in L. biflexa cells when both dCas9 and single-guide RNA (sgRNA) targeting the coding strand of the β-galactosidase gene were expressed simultaneously. Furthermore, when the system was applied for silencing the dnaK gene, no colonies were recovered, indicating that DnaK protein is essential in Leptospira. In addition, flagellar motor switch FliG gene silencing resulted in reduced bacterial motility. To the best of our knowledge, this is the first work applying the CRISPRi system in Leptospira and spirochetes in general, expanding the tools available for understanding leptospiral biology.
82 schema:genre research_article
83 schema:inLanguage en
84 schema:isAccessibleForFree true
85 schema:isPartOf N82e2d37330e942028a32718665c0f776
86 N9417abe3bf514213a5ae8c9850219304
87 sg:journal.1045337
88 schema:name Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in Leptospira
89 schema:pagination 1839
90 schema:productId N2fbf03ecd6a8408dba7d1323d67971bd
91 N49ba9f0a3bfa4e059b3cef8c0d62546a
92 N5e3cd180536344f5bc96b7755d62c648
93 N5ec6181f572746baa8e6eb28b4b820f8
94 N8af956e6d66b4893810cce5953b1af90
95 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112063978
96 https://doi.org/10.1038/s41598-018-37949-x
97 schema:sdDatePublished 2019-04-11T09:09
98 schema:sdLicense https://scigraph.springernature.com/explorer/license/
99 schema:sdPublisher Nd3ddb2599799436aaa19d888d152b1dd
100 schema:url https://www.nature.com/articles/s41598-018-37949-x
101 sgo:license sg:explorer/license/
102 sgo:sdDataset articles
103 rdf:type schema:ScholarlyArticle
104 N29bd0bc176454a2191b411868daef324 rdf:first Ne1c89c23275e487ca044d4c68edd8379
105 rdf:rest Nd6074d527c024166ba5f6877ec2d9bb3
106 N2fbf03ecd6a8408dba7d1323d67971bd schema:name nlm_unique_id
107 schema:value 101563288
108 rdf:type schema:PropertyValue
109 N49ba9f0a3bfa4e059b3cef8c0d62546a schema:name dimensions_id
110 schema:value pub.1112063978
111 rdf:type schema:PropertyValue
112 N4e435148133f4faaa791e0ee8a19b8e8 schema:affiliation https://www.grid.ac/institutes/grid.418514.d
113 schema:familyName Nascimento
114 schema:givenName A. L. T. O.
115 rdf:type schema:Person
116 N5e3cd180536344f5bc96b7755d62c648 schema:name doi
117 schema:value 10.1038/s41598-018-37949-x
118 rdf:type schema:PropertyValue
119 N5ec6181f572746baa8e6eb28b4b820f8 schema:name pubmed_id
120 schema:value 30755626
121 rdf:type schema:PropertyValue
122 N6918eb5b5a84471992b69791f4649d3a rdf:first N4e435148133f4faaa791e0ee8a19b8e8
123 rdf:rest rdf:nil
124 N7b25cfb57e52407da4129808920b3cec rdf:first Na2e14e05e5ab474b942f649d43ed065c
125 rdf:rest N7e789cfccd4a42f68d8ef8ca89c9b79a
126 N7e789cfccd4a42f68d8ef8ca89c9b79a rdf:first Na1364b2b209f44b7a5ea14469f2cb9b3
127 rdf:rest N29bd0bc176454a2191b411868daef324
128 N82e2d37330e942028a32718665c0f776 schema:volumeNumber 9
129 rdf:type schema:PublicationVolume
130 N8af956e6d66b4893810cce5953b1af90 schema:name readcube_id
131 schema:value 1e332f4746348ffdca06f1a5d7797434779f688baba55052df2f1954ef85d8b9
132 rdf:type schema:PropertyValue
133 N9417abe3bf514213a5ae8c9850219304 schema:issueNumber 1
134 rdf:type schema:PublicationIssue
135 N9a4e0d5c9e1b4cb581968affc49c578f schema:affiliation https://www.grid.ac/institutes/grid.418514.d
136 schema:familyName Fernandes
137 schema:givenName L. G. V.
138 rdf:type schema:Person
139 Na1364b2b209f44b7a5ea14469f2cb9b3 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
140 schema:familyName Vasconcellos
141 schema:givenName S. A.
142 rdf:type schema:Person
143 Na2e14e05e5ab474b942f649d43ed065c schema:affiliation https://www.grid.ac/institutes/grid.412257.7
144 schema:familyName Guaman
145 schema:givenName L. P.
146 rdf:type schema:Person
147 Nccecbd792a164e5eb60ed67b104f4426 schema:affiliation https://www.grid.ac/institutes/grid.428999.7
148 schema:familyName Picardeau
149 schema:givenName M.
150 rdf:type schema:Person
151 Nd3ddb2599799436aaa19d888d152b1dd schema:name Springer Nature - SN SciGraph project
152 rdf:type schema:Organization
153 Nd6074d527c024166ba5f6877ec2d9bb3 rdf:first Nccecbd792a164e5eb60ed67b104f4426
154 rdf:rest N6918eb5b5a84471992b69791f4649d3a
155 Ne1c89c23275e487ca044d4c68edd8379 schema:affiliation https://www.grid.ac/institutes/grid.11899.38
156 schema:familyName Heinemann
157 schema:givenName Marcos B.
158 rdf:type schema:Person
159 Nf877960a4f9e4652aa5fdd46c1afeb25 rdf:first N9a4e0d5c9e1b4cb581968affc49c578f
160 rdf:rest N7b25cfb57e52407da4129808920b3cec
161 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
162 schema:name Biological Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
165 schema:name Genetics
166 rdf:type schema:DefinedTerm
167 sg:grant.4543480 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-018-37949-x
168 rdf:type schema:MonetaryGrant
169 sg:grant.4604028 http://pending.schema.org/fundedItem sg:pub.10.1038/s41598-018-37949-x
170 rdf:type schema:MonetaryGrant
171 sg:journal.1045337 schema:issn 2045-2322
172 schema:name Scientific Reports
173 rdf:type schema:Periodical
174 sg:pub.10.1007/978-1-61779-603-6_9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022263055
175 https://doi.org/10.1007/978-1-61779-603-6_9
176 rdf:type schema:CreativeWork
177 sg:pub.10.1007/s00253-018-8927-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103183290
178 https://doi.org/10.1007/s00253-018-8927-3
179 rdf:type schema:CreativeWork
180 sg:pub.10.1007/s00335-015-9565-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1032581219
181 https://doi.org/10.1007/s00335-015-9565-z
182 rdf:type schema:CreativeWork
183 sg:pub.10.1007/s40484-014-0030-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014529473
184 https://doi.org/10.1007/s40484-014-0030-x
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/cr.2013.146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007939040
187 https://doi.org/10.1038/cr.2013.146
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/nature09523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009515358
190 https://doi.org/10.1038/nature09523
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/nbt.2650 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040481921
193 https://doi.org/10.1038/nbt.2650
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/nbt.3011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007861343
196 https://doi.org/10.1038/nbt.3011
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/ncomms7267 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014345430
199 https://doi.org/10.1038/ncomms7267
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/nsmb915 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029773752
202 https://doi.org/10.1038/nsmb915
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/s41579-018-0002-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101821595
205 https://doi.org/10.1038/s41579-018-0002-7
206 rdf:type schema:CreativeWork
207 sg:pub.10.1186/s12934-017-0802-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1092515990
208 https://doi.org/10.1186/s12934-017-0802-x
209 rdf:type schema:CreativeWork
210 https://app.dimensions.ai/details/publication/pub.1079196676 schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1079425218 schema:CreativeWork
212 https://doi.org/10.1002/9780471729259.mc12e04s05 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005174790
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1002/biot.201800121 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104374989
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0035-9203(70)90087-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029551515
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/j.cell.2013.02.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008291683
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.cell.2016.05.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008876849
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/j.chom.2012.06.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025013559
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/j.dnarep.2004.06.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029232834
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.jmb.2006.10.046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045885195
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.resmic.2004.09.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032407606
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.tibtech.2013.04.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031527623
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.vetmic.2009.03.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053273451
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/s1473-3099(03)00830-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018864760
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1021/sb500351f schema:sameAs https://app.dimensions.ai/details/publication/pub.1003383831
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1046/j.1365-2958.2001.02374.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035997180
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1074/jbc.m117.804443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092730856
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1086/427110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009670564
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1093/nar/gkt135 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042813151
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1093/nar/gkt520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002402497
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1093/nar/gku241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038476578
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1093/nar/gku410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020146143
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1093/nar/gkw223 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025545721
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1093/nar/gkw231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030550273
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1093/nar/gkw625 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051483332
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1097/01.qco.0000178824.05715.2c schema:sameAs https://app.dimensions.ai/details/publication/pub.1034132415
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1099/ijs.0.047324-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060385131
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1101/gad.1631908 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011253653
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1101/gr.181001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034860782
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1111/j.1365-2958.2010.07078.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005685461
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1111/j.1365-2958.2010.07360.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051916975
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1111/j.1365-2958.2012.07985.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018329845
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1111/j.1574-6968.2009.01542.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034029086
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1126/science.1138140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036312168
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1126/science.1225829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041850060
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1126/science.1232033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022072971
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1128/aem.00173-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016224700
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1128/aem.00291-17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084852307
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1128/aem.00873-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051464659
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1128/aem.01619-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053281388
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1128/aem.02172-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003614845
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1128/aem.02202-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040489672
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1128/aem.04023-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035109565
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1128/iai.00131-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000416889
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1128/iai.00989-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009682018
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1128/iai.01293-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030914414
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1128/iai.01546-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016289015
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1128/jb.00575-17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100257882
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1128/jb.02042-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020478365
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1128/jb.182.20.5700-5705.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017485070
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1128/jb.186.7.2164-2172.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044705615
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1128/mbio.00928-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039604015
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1128/mcb.15.4.1968 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034391289
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1146/annurev.biochem.052308.093131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022097335
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1371/journal.pgen.1007148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100085807
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1371/journal.pntd.0003952 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035650849
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1371/journal.pone.0136690 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010259449
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1371/journal.pone.0194923 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103202960
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1371/journal.ppat.0030097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046412884
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1590/s0100-879x2004000400003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040970778
327 rdf:type schema:CreativeWork
328 https://doi.org/10.3389/fcimb.2017.00010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049896955
329 rdf:type schema:CreativeWork
330 https://doi.org/10.3389/fcimb.2017.00214 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085599291
331 rdf:type schema:CreativeWork
332 https://doi.org/10.3389/fcimb.2018.00130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103857347
333 rdf:type schema:CreativeWork
334 https://www.grid.ac/institutes/grid.11899.38 schema:alternateName University of Sao Paulo
335 schema:name Laboratório de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterinária e Zootecnia, USP, Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508-270, Sao Paulo, SP, Brazil
336 rdf:type schema:Organization
337 https://www.grid.ac/institutes/grid.412257.7 schema:alternateName Universidad Tecnológica Equinoccial
338 schema:name Universidad Tecnológica Equinoccial, Centro de Investigación Biomédica, Facultad de Ciencias de la Salud Eugenio Espejo, Avenida Mariscal Sucre y Mariana de Jesús. Campus Occidental, 170105, Quito, Ecuador
339 rdf:type schema:Organization
340 https://www.grid.ac/institutes/grid.418514.d schema:alternateName Instituto Butantan
341 schema:name Laboratório Especial de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brasil, 1500, 05503-900, Sao Paulo, SP, Brazil
342 rdf:type schema:Organization
343 https://www.grid.ac/institutes/grid.428999.7 schema:alternateName Institut Pasteur
344 schema:name Institut Pasteur, Biology of Spirochetes Unit, 25 rue du Dr Roux, 75723, Paris, France
345 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...