Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Wei Hou, Yangyang Li, Wenjie Kang, Xin Wang, Xuping Wu, Shouyu Wang, Fei Liu

ABSTRACT

In order to study the infection mechanism of porcine epidemic diarrhea virus (PEDV), which causes porcine epidemic diarrhea, a highly contagious enteric disease, we combined quantum dot labeled method, which could hold intact infectivity of the labeled viruses to the largest extent, with the single particle tracking technique to dynamically and globally visualize the transport behaviors of PEDVs in live Vero cells. Our results were the first time to uncover the dynamic characteristics of PEDVs moving along the microtubules in the host cells. It is found that PEDVs kept restricted motion mode with a relatively stable speed in the cell membrane region; while performed a slow-fast-slow velocity pattern with different motion modes in the cell cytoplasm region and near the microtubule organizing center region. In addition, the return movements of small amount of PEDVs were also observed in the live cells. Collectively, our work is crucial for understanding the movement mechanisms of PEDV in the live cells, and the proposed work also provided important references for further analysis and study on the infection mechanism of PEDVs. More... »

PAGES

1307

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-37789-9

DOI

http://dx.doi.org/10.1038/s41598-018-37789-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111913389

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30718724


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Nanjing Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.27871.3b", 
          "name": [
            "Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hou", 
        "givenName": "Wei", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanjing Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.27871.3b", 
          "name": [
            "Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Yangyang", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanjing Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.27871.3b", 
          "name": [
            "Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Wenjie", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanjing Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.27871.3b", 
          "name": [
            "Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Xin", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanjing Second Hospital", 
          "id": "https://www.grid.ac/institutes/grid.452675.7", 
          "name": [
            "The Second Hospital of Nanjing Affiliated to Southeast University, 210003, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Xuping", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China", 
            "Computational Optics Laboratory, School of Science, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Shouyu", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanjing Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.27871.3b", 
          "name": [
            "Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Fei", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1083/jcb.200203150", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001582225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004843793", 
          "https://doi.org/10.1038/nrm777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004843793", 
          "https://doi.org/10.1038/nrm777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c4an00038b", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007947496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac500640u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008089521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.76.7.3282-3291.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008768403"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.78.15.7990-8001.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010887620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0050183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011683904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.micinf.2004.05.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012033319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja074936k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015799829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja074936k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015799829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.77.19.10270-10279.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015899024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2005.12.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016706270"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.02.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016762783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35074539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017645872", 
          "https://doi.org/10.1038/35074539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35074539", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017645872", 
          "https://doi.org/10.1038/35074539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biomaterials.2016.08.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017837624"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virol.2007.03.031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018436567"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.02.046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019127515"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2004.06.045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020025984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/smll.201400944", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020784790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.02.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021629494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1000244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023308904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.76.17.8820-8833.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024705850"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ceb.2007.11.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027931985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0170126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028038000"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028674181", 
          "https://doi.org/10.1038/ni1019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028674181", 
          "https://doi.org/10.1038/ni1019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0042-6822(87)90075-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029377162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1000702", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029678759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2005.05.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031260399"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virusres.2014.07.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033517926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037587534", 
          "https://doi.org/10.1038/nmeth.1321"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037587534", 
          "https://doi.org/10.1038/nmeth.1321"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.21.6.2133-2143.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037934665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.00430-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040602742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2011.07.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043098168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymeth.2011.07.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043098168"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biomaterials.2011.06.046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044210453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.00300-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045681650"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1064103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045876772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0832269100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048354193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.279.5350.519", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048507310"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/smll.201303532", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049411898"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2011.283", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050786840"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02788556", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052501389", 
          "https://doi.org/10.1007/bf02788556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac301918t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055003247"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acsnano.5b03256", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055137178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acsnano.5b06438", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055137520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acsnano.6b02462", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055137926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/nn2031353", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056223778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/nn8002136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056226821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/10430340050016238", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059201998"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/jgv.0.000563", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060390155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.aje.a118408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083592874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/acsnano.6b07853", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084125151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.00202-17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084222038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/c7nr02162c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085391387"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "In order to study the infection mechanism of porcine epidemic diarrhea virus (PEDV), which causes porcine epidemic diarrhea, a highly contagious enteric disease, we combined quantum dot labeled method, which could hold intact infectivity of the labeled viruses to the largest extent, with the single particle tracking technique to dynamically and globally visualize the transport behaviors of PEDVs in live Vero cells. Our results were the first time to uncover the dynamic characteristics of PEDVs moving along the microtubules in the host cells. It is found that PEDVs kept restricted motion mode with a relatively stable speed in the cell membrane region; while performed a slow-fast-slow velocity pattern with different motion modes in the cell cytoplasm region and near the microtubule organizing center region. In addition, the return movements of small amount of PEDVs were also observed in the live cells. Collectively, our work is crucial for understanding the movement mechanisms of PEDV in the live cells, and the proposed work also provided important references for further analysis and study on the infection mechanism of PEDVs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-37789-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking", 
    "pagination": "1307", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "898c9a61b3a1fbb46a1dec994036ea3dfcf3d029116c849925224966e5080a29"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30718724"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-37789-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111913389"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-37789-9", 
      "https://app.dimensions.ai/details/publication/pub.1111913389"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000330_0000000330/records_116370_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-37789-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37789-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37789-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37789-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37789-9'


 

This table displays all metadata directly associated to this object as RDF triples.

271 TRIPLES      21 PREDICATES      81 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-37789-9 schema:about anzsrc-for:11
2 anzsrc-for:1108
3 schema:author Nf53f5ea570b8453ea15497cddbdc7d3d
4 schema:citation sg:pub.10.1007/bf02788556
5 sg:pub.10.1038/35074539
6 sg:pub.10.1038/ni1019
7 sg:pub.10.1038/nmeth.1321
8 sg:pub.10.1038/nrm777
9 https://doi.org/10.1002/smll.201303532
10 https://doi.org/10.1002/smll.201400944
11 https://doi.org/10.1016/0042-6822(87)90075-4
12 https://doi.org/10.1016/j.biomaterials.2011.06.046
13 https://doi.org/10.1016/j.biomaterials.2016.08.013
14 https://doi.org/10.1016/j.ceb.2007.11.006
15 https://doi.org/10.1016/j.cell.2005.12.038
16 https://doi.org/10.1016/j.cell.2006.02.007
17 https://doi.org/10.1016/j.cell.2006.02.018
18 https://doi.org/10.1016/j.cell.2009.02.046
19 https://doi.org/10.1016/j.cub.2004.06.045
20 https://doi.org/10.1016/j.micinf.2004.05.002
21 https://doi.org/10.1016/j.tim.2005.05.010
22 https://doi.org/10.1016/j.virol.2007.03.031
23 https://doi.org/10.1016/j.virusres.2014.07.022
24 https://doi.org/10.1016/j.ymeth.2011.07.009
25 https://doi.org/10.1021/ac301918t
26 https://doi.org/10.1021/ac500640u
27 https://doi.org/10.1021/acsnano.5b03256
28 https://doi.org/10.1021/acsnano.5b06438
29 https://doi.org/10.1021/acsnano.6b02462
30 https://doi.org/10.1021/acsnano.6b07853
31 https://doi.org/10.1021/ja074936k
32 https://doi.org/10.1021/nn2031353
33 https://doi.org/10.1021/nn8002136
34 https://doi.org/10.1038/emboj.2011.283
35 https://doi.org/10.1039/c4an00038b
36 https://doi.org/10.1039/c7nr02162c
37 https://doi.org/10.1073/pnas.0832269100
38 https://doi.org/10.1083/jcb.200203150
39 https://doi.org/10.1089/10430340050016238
40 https://doi.org/10.1093/oxfordjournals.aje.a118408
41 https://doi.org/10.1099/jgv.0.000563
42 https://doi.org/10.1126/science.1064103
43 https://doi.org/10.1126/science.279.5350.519
44 https://doi.org/10.1128/jvi.00202-17
45 https://doi.org/10.1128/jvi.00300-07
46 https://doi.org/10.1128/jvi.00430-15
47 https://doi.org/10.1128/jvi.76.17.8820-8833.2002
48 https://doi.org/10.1128/jvi.76.7.3282-3291.2002
49 https://doi.org/10.1128/jvi.77.19.10270-10279.2003
50 https://doi.org/10.1128/jvi.78.15.7990-8001.2004
51 https://doi.org/10.1128/mcb.21.6.2133-2143.2001
52 https://doi.org/10.1371/journal.pbio.0050183
53 https://doi.org/10.1371/journal.pone.0170126
54 https://doi.org/10.1371/journal.ppat.1000244
55 https://doi.org/10.1371/journal.ppat.1000702
56 schema:datePublished 2019-12
57 schema:datePublishedReg 2019-12-01
58 schema:description In order to study the infection mechanism of porcine epidemic diarrhea virus (PEDV), which causes porcine epidemic diarrhea, a highly contagious enteric disease, we combined quantum dot labeled method, which could hold intact infectivity of the labeled viruses to the largest extent, with the single particle tracking technique to dynamically and globally visualize the transport behaviors of PEDVs in live Vero cells. Our results were the first time to uncover the dynamic characteristics of PEDVs moving along the microtubules in the host cells. It is found that PEDVs kept restricted motion mode with a relatively stable speed in the cell membrane region; while performed a slow-fast-slow velocity pattern with different motion modes in the cell cytoplasm region and near the microtubule organizing center region. In addition, the return movements of small amount of PEDVs were also observed in the live cells. Collectively, our work is crucial for understanding the movement mechanisms of PEDV in the live cells, and the proposed work also provided important references for further analysis and study on the infection mechanism of PEDVs.
59 schema:genre research_article
60 schema:inLanguage en
61 schema:isAccessibleForFree true
62 schema:isPartOf N0658f5f15c854671a91841385921f1d2
63 N550ede5c05a246cd9db73a2f4fb91481
64 sg:journal.1045337
65 schema:name Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking
66 schema:pagination 1307
67 schema:productId N172d81d266c847d18c609c63e1c33462
68 N40b925545f8644b3b2c02086acf48933
69 N482367672bcb433a9f53385a73b98e42
70 N7bf3c80548884eefb82ea8b55233ad91
71 Ndb97d66b31304f37a7a6b8fcd7468f28
72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111913389
73 https://doi.org/10.1038/s41598-018-37789-9
74 schema:sdDatePublished 2019-04-11T09:01
75 schema:sdLicense https://scigraph.springernature.com/explorer/license/
76 schema:sdPublisher N10ad7efcf65948d9b4b54a1de7ed0b48
77 schema:url https://www.nature.com/articles/s41598-018-37789-9
78 sgo:license sg:explorer/license/
79 sgo:sdDataset articles
80 rdf:type schema:ScholarlyArticle
81 N0658f5f15c854671a91841385921f1d2 schema:volumeNumber 9
82 rdf:type schema:PublicationVolume
83 N10ad7efcf65948d9b4b54a1de7ed0b48 schema:name Springer Nature - SN SciGraph project
84 rdf:type schema:Organization
85 N12e726d96003498fa644761a84ea3b49 rdf:first N4828001051604b9296adb67ae45d68ef
86 rdf:rest Nc8166ff1f955444cbff63abb4e5c21c5
87 N172d81d266c847d18c609c63e1c33462 schema:name nlm_unique_id
88 schema:value 101563288
89 rdf:type schema:PropertyValue
90 N242695c63cfa475b9caa30dfa39213db rdf:first Na7920b5f13f042928ab7d98bdc4d2ea8
91 rdf:rest Na33d46ded29b46e9b08b04883288b97c
92 N40b925545f8644b3b2c02086acf48933 schema:name pubmed_id
93 schema:value 30718724
94 rdf:type schema:PropertyValue
95 N482367672bcb433a9f53385a73b98e42 schema:name dimensions_id
96 schema:value pub.1111913389
97 rdf:type schema:PropertyValue
98 N4828001051604b9296adb67ae45d68ef schema:affiliation https://www.grid.ac/institutes/grid.27871.3b
99 schema:familyName Li
100 schema:givenName Yangyang
101 rdf:type schema:Person
102 N550ede5c05a246cd9db73a2f4fb91481 schema:issueNumber 1
103 rdf:type schema:PublicationIssue
104 N6ca9e5866faa4d53a5fcc7bd2839da2a rdf:first Nf2061d9234e449e5b9655f5aa65f2aa0
105 rdf:rest Ne4af8c907c734826a1f346b3a9e5ef18
106 N7bf3c80548884eefb82ea8b55233ad91 schema:name doi
107 schema:value 10.1038/s41598-018-37789-9
108 rdf:type schema:PropertyValue
109 N9b054805aaa246f5a1131857e7b9a24b schema:affiliation https://www.grid.ac/institutes/grid.452675.7
110 schema:familyName Wu
111 schema:givenName Xuping
112 rdf:type schema:Person
113 Na33d46ded29b46e9b08b04883288b97c rdf:first N9b054805aaa246f5a1131857e7b9a24b
114 rdf:rest N6ca9e5866faa4d53a5fcc7bd2839da2a
115 Na7920b5f13f042928ab7d98bdc4d2ea8 schema:affiliation https://www.grid.ac/institutes/grid.27871.3b
116 schema:familyName Wang
117 schema:givenName Xin
118 rdf:type schema:Person
119 Nb404e46d11b64a5781e66b040bc999d7 schema:affiliation https://www.grid.ac/institutes/grid.27871.3b
120 schema:familyName Liu
121 schema:givenName Fei
122 rdf:type schema:Person
123 Nc8166ff1f955444cbff63abb4e5c21c5 rdf:first Ne611e889e76043da8de0fbcd031ddd8d
124 rdf:rest N242695c63cfa475b9caa30dfa39213db
125 Nda3c0cbb6fe141b08259480d6c73d34b schema:affiliation https://www.grid.ac/institutes/grid.27871.3b
126 schema:familyName Hou
127 schema:givenName Wei
128 rdf:type schema:Person
129 Ndb97d66b31304f37a7a6b8fcd7468f28 schema:name readcube_id
130 schema:value 898c9a61b3a1fbb46a1dec994036ea3dfcf3d029116c849925224966e5080a29
131 rdf:type schema:PropertyValue
132 Ne4af8c907c734826a1f346b3a9e5ef18 rdf:first Nb404e46d11b64a5781e66b040bc999d7
133 rdf:rest rdf:nil
134 Ne611e889e76043da8de0fbcd031ddd8d schema:affiliation https://www.grid.ac/institutes/grid.27871.3b
135 schema:familyName Kang
136 schema:givenName Wenjie
137 rdf:type schema:Person
138 Nf2061d9234e449e5b9655f5aa65f2aa0 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
139 schema:familyName Wang
140 schema:givenName Shouyu
141 rdf:type schema:Person
142 Nf53f5ea570b8453ea15497cddbdc7d3d rdf:first Nda3c0cbb6fe141b08259480d6c73d34b
143 rdf:rest N12e726d96003498fa644761a84ea3b49
144 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
145 schema:name Medical and Health Sciences
146 rdf:type schema:DefinedTerm
147 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
148 schema:name Medical Microbiology
149 rdf:type schema:DefinedTerm
150 sg:journal.1045337 schema:issn 2045-2322
151 schema:name Scientific Reports
152 rdf:type schema:Periodical
153 sg:pub.10.1007/bf02788556 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052501389
154 https://doi.org/10.1007/bf02788556
155 rdf:type schema:CreativeWork
156 sg:pub.10.1038/35074539 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017645872
157 https://doi.org/10.1038/35074539
158 rdf:type schema:CreativeWork
159 sg:pub.10.1038/ni1019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028674181
160 https://doi.org/10.1038/ni1019
161 rdf:type schema:CreativeWork
162 sg:pub.10.1038/nmeth.1321 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037587534
163 https://doi.org/10.1038/nmeth.1321
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/nrm777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004843793
166 https://doi.org/10.1038/nrm777
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1002/smll.201303532 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049411898
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1002/smll.201400944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020784790
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1016/0042-6822(87)90075-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029377162
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/j.biomaterials.2011.06.046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044210453
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/j.biomaterials.2016.08.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017837624
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.ceb.2007.11.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027931985
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/j.cell.2005.12.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016706270
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/j.cell.2006.02.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016762783
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/j.cell.2006.02.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021629494
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/j.cell.2009.02.046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019127515
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.cub.2004.06.045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020025984
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.micinf.2004.05.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012033319
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.tim.2005.05.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031260399
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.virol.2007.03.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018436567
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.virusres.2014.07.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033517926
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.ymeth.2011.07.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043098168
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1021/ac301918t schema:sameAs https://app.dimensions.ai/details/publication/pub.1055003247
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1021/ac500640u schema:sameAs https://app.dimensions.ai/details/publication/pub.1008089521
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1021/acsnano.5b03256 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055137178
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1021/acsnano.5b06438 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055137520
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1021/acsnano.6b02462 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055137926
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1021/acsnano.6b07853 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084125151
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1021/ja074936k schema:sameAs https://app.dimensions.ai/details/publication/pub.1015799829
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1021/nn2031353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056223778
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1021/nn8002136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056226821
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1038/emboj.2011.283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050786840
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1039/c4an00038b schema:sameAs https://app.dimensions.ai/details/publication/pub.1007947496
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1039/c7nr02162c schema:sameAs https://app.dimensions.ai/details/publication/pub.1085391387
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1073/pnas.0832269100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048354193
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1083/jcb.200203150 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001582225
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1089/10430340050016238 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059201998
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1093/oxfordjournals.aje.a118408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083592874
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1099/jgv.0.000563 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060390155
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1126/science.1064103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045876772
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1126/science.279.5350.519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048507310
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1128/jvi.00202-17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084222038
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1128/jvi.00300-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045681650
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1128/jvi.00430-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040602742
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1128/jvi.76.17.8820-8833.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024705850
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1128/jvi.76.7.3282-3291.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008768403
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1128/jvi.77.19.10270-10279.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015899024
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1128/jvi.78.15.7990-8001.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010887620
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1128/mcb.21.6.2133-2143.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037934665
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1371/journal.pbio.0050183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011683904
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1371/journal.pone.0170126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028038000
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1371/journal.ppat.1000244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023308904
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1371/journal.ppat.1000702 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029678759
261 rdf:type schema:CreativeWork
262 https://www.grid.ac/institutes/grid.258151.a schema:alternateName Jiangnan University
263 schema:name Computational Optics Laboratory, School of Science, Jiangnan University, 214122, Wuxi, China
264 Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China
265 rdf:type schema:Organization
266 https://www.grid.ac/institutes/grid.27871.3b schema:alternateName Nanjing Agricultural University
267 schema:name Joint International Research Laboratory of Animal Health and Food Safety, Single Molecule Nanometry Laboratory (Sinmolab) & Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, 210095, Nanjing, China
268 rdf:type schema:Organization
269 https://www.grid.ac/institutes/grid.452675.7 schema:alternateName Nanjing Second Hospital
270 schema:name The Second Hospital of Nanjing Affiliated to Southeast University, 210003, Nanjing, China
271 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...