N-acetylcysteine modulates lipopolysaccharide-induced intestinal dysfunction View Full Text


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Article Info

DATE

2019-12

AUTHORS

Sang In Lee, Kyung Soo Kang

ABSTRACT

The gastrointestinal epithelium functions in nutrient absorption and pathogens barrier and its dysfunction directly affects livestock performance. N-Acetylcysteine (NAC) improves mucosal function, but its effects on intestinal functions at the molecular level remain unclear. Here, we performed gene expression profiling of the pig small intestine after dietary NAC treatment under LPS challenge and investigated the effects of NAC on intestinal epithelial cells in vitro. Dietary NAC supplementation under LPS challenge altered the small intestine expression of 959 genes related to immune response, inflammatory response, oxidation-reduction process, cytokine-cytokine receptor interaction, and the cytokine-mediated signalling, Toll-like receptor signalling pathway, Jak-STAT signalling pathway, and TNF signalling pathway. We then analysed the expression patterns of the top 10 altered genes, and found that NAC markedly stimulated HMGCS3 and LDHC expression in IPEC-J2 cells. In vitro, NAC pre-treatment significantly reduced TNF-α and NF-κB, TNF-α, IFN-γ, and IL-6 expression in LPS-induced IPEC-J2 cells. NAC treatment also significantly reduced oxidative stress in LPS-induced IPEC-J2 cells and alleviated intestinal barrier function and wound healing. Thus, NAC as a feed additive can enhance livestock intestinal health by modulating intestinal inflammation, permeability, and wound healing under LPS-induced dysfunction, improving our molecular understanding of the effects of NAC on the intestine. More... »

PAGES

1004

References to SciGraph publications

  • 2007-08. Rho activation regulates CXCL12 chemokine stimulated actin rearrangement and restitution in model intestinal epithelia in LABORATORY INVESTIGATION
  • 2003-01. Molecular mechanisms of N-acetylcysteine actions in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2017-12. N-Acetylcysteine improves intestinal function in lipopolysaccharides-challenged piglets through multiple signaling pathways in AMINO ACIDS
  • 2016-10. Human Intestinal Barrier Function in Health and Disease in CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
  • 2012-09. Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide in AMINO ACIDS
  • 2005-12. The Antioxidant Role of Glutathione and N-Acetyl-Cysteine Supplements and Exercise-Induced Oxidative Stress in JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION
  • 2006. TLR Signaling in FROM INNATE IMMUNITY TO IMMUNOLOGICAL MEMORY
  • 2018-12. Nucleotide-mediated SPDEF modulates TFF3-mediated wound healing and intestinal barrier function during the weaning process in SCIENTIFIC REPORTS
  • 2009-05. Amino acids: metabolism, functions, and nutrition in AMINO ACIDS
  • 2017-12. Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic in SCIENTIFIC REPORTS
  • 2012-12. Oxidative Stress and Antioxidant Defense in WORLD ALLERGY ORGANIZATION JOURNAL
  • 2017-09. Layered defense: how mucus and tight junctions seal the intestinal barrier in JOURNAL OF MOLECULAR MEDICINE
  • 2002-12. Intraluminal casein model of necrotizing enterocolitis for assessment of mucosal destruction, bacterial translocation, and the effects of allopurinol and N-acetylcysteine in PEDIATRIC SURGERY INTERNATIONAL
  • Journal

    TITLE

    Scientific Reports

    ISSUE

    1

    VOLUME

    9

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-37296-x

    DOI

    http://dx.doi.org/10.1038/s41598-018-37296-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1111778122

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30700808


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