Ontology type: schema:ScholarlyArticle Open Access: True
2019-12
AUTHORSSang In Lee, Kyung Soo Kang
ABSTRACTThe gastrointestinal epithelium functions in nutrient absorption and pathogens barrier and its dysfunction directly affects livestock performance. N-Acetylcysteine (NAC) improves mucosal function, but its effects on intestinal functions at the molecular level remain unclear. Here, we performed gene expression profiling of the pig small intestine after dietary NAC treatment under LPS challenge and investigated the effects of NAC on intestinal epithelial cells in vitro. Dietary NAC supplementation under LPS challenge altered the small intestine expression of 959 genes related to immune response, inflammatory response, oxidation-reduction process, cytokine-cytokine receptor interaction, and the cytokine-mediated signalling, Toll-like receptor signalling pathway, Jak-STAT signalling pathway, and TNF signalling pathway. We then analysed the expression patterns of the top 10 altered genes, and found that NAC markedly stimulated HMGCS3 and LDHC expression in IPEC-J2 cells. In vitro, NAC pre-treatment significantly reduced TNF-α and NF-κB, TNF-α, IFN-γ, and IL-6 expression in LPS-induced IPEC-J2 cells. NAC treatment also significantly reduced oxidative stress in LPS-induced IPEC-J2 cells and alleviated intestinal barrier function and wound healing. Thus, NAC as a feed additive can enhance livestock intestinal health by modulating intestinal inflammation, permeability, and wound healing under LPS-induced dysfunction, improving our molecular understanding of the effects of NAC on the intestine. More... »
PAGES1004
http://scigraph.springernature.com/pub.10.1038/s41598-018-37296-x
DOIhttp://dx.doi.org/10.1038/s41598-018-37296-x
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1111778122
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/30700808
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Dankook University",
"id": "https://www.grid.ac/institutes/grid.411982.7",
"name": [
"Department of Animal Resource and Science, Dankook University, 330-714, Cheonan, Chungnam, Republic of Korea"
],
"type": "Organization"
},
"familyName": "Lee",
"givenName": "Sang In",
"type": "Person"
},
{
"affiliation": {
"name": [
"Bio Division, Medikinetics, Inc., Hansan-gil, Pyeongtaek-si, 17792, Gyeonggi-do, Republic of Korea"
],
"type": "Organization"
},
"familyName": "Kang",
"givenName": "Kyung Soo",
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/labinvest.3700595",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001131498",
"https://doi.org/10.1038/labinvest.3700595"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jpba.2009.04.019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002317039"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1136/gut.52.10.1472",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002586296"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1017/s0007114515004857",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004284344"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1017/s0007114510005660",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006770881"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.pharmtox.39.1.67",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008195365"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1097/wox.0b013e3182439613",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009320857",
"https://doi.org/10.1097/wox.0b013e3182439613"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/wox.0b013e3182439613",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009320857"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1462-5822.2007.00965.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009513468"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.mog.0000203864.48255.4f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011829589"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.mog.0000203864.48255.4f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011829589"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.mog.0000203864.48255.4f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011829589"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.iy.12.040194.001041",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012349720"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1155/2016/8364279",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015799947"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cbi.2005.12.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017043107"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/mog.0b013e328324f857",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017138585"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/mog.0b013e328324f857",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017138585"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/mog.0b013e328324f857",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017138585"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/mog.0b013e328324f857",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017138585"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-011-1191-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020101052",
"https://doi.org/10.1007/s00726-011-1191-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-011-1191-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020101052",
"https://doi.org/10.1007/s00726-011-1191-9"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.semcdb.2012.03.014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021725146"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-009-0269-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026477501",
"https://doi.org/10.1007/s00726-009-0269-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-009-0269-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026477501",
"https://doi.org/10.1007/s00726-009-0269-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-009-0269-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026477501",
"https://doi.org/10.1007/s00726-009-0269-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-009-0269-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026477501",
"https://doi.org/10.1007/s00726-009-0269-0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/meth.2001.1262",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027621591"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/meth.2001.1262",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027621591"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/3-540-32636-7_1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028646878",
"https://doi.org/10.1007/3-540-32636-7_1"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jaci.2009.05.038",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028864249"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s000180300001",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033620600",
"https://doi.org/10.1007/s000180300001"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1084/jem.20082683",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034156043"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ctg.2016.54",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035158219",
"https://doi.org/10.1038/ctg.2016.54"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2982.2012.01921.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038877787"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1550-2783-2-2-38",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041725353",
"https://doi.org/10.1186/1550-2783-2-2-38"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bbamem.2008.07.015",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047300411"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.gastro.2005.05.054",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1054735476"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.asn.0000107562.68920.92",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060319469"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.asn.0000107562.68920.92",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060319469"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.asn.0000107562.68920.92",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060319469"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2527/jas.2013-6755",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1070889074"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2741/4342",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1070916518"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3748/wjg.v17.i17.2161",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1071367538"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00383-002-0871-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1075241382",
"https://doi.org/10.1007/s00383-002-0871-7"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jn/134.3.489",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1076802125"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2527/jas.2012-4444",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1078609340"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-017-2389-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084023225",
"https://doi.org/10.1007/s00726-017-2389-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00726-017-2389-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1084023225",
"https://doi.org/10.1007/s00726-017-2389-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00109-017-1557-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090659379",
"https://doi.org/10.1007/s00109-017-1557-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00109-017-1557-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1090659379",
"https://doi.org/10.1007/s00109-017-1557-x"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-017-16158-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1092669273",
"https://doi.org/10.1038/s41598-017-16158-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-018-23218-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101561199",
"https://doi.org/10.1038/s41598-018-23218-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-018-23218-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101561199",
"https://doi.org/10.1038/s41598-018-23218-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/s41598-018-23218-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1101561199",
"https://doi.org/10.1038/s41598-018-23218-4"
],
"type": "CreativeWork"
}
],
"datePublished": "2019-12",
"datePublishedReg": "2019-12-01",
"description": "The gastrointestinal epithelium functions in nutrient absorption and pathogens barrier and its dysfunction directly affects livestock performance. N-Acetylcysteine (NAC) improves mucosal function, but its effects on intestinal functions at the molecular level remain unclear. Here, we performed gene expression profiling of the pig small intestine after dietary NAC treatment under LPS challenge and investigated the effects of NAC on intestinal epithelial cells in vitro. Dietary NAC supplementation under LPS challenge altered the small intestine expression of 959 genes related to immune response, inflammatory response, oxidation-reduction process, cytokine-cytokine receptor interaction, and the cytokine-mediated signalling, Toll-like receptor signalling pathway, Jak-STAT signalling pathway, and TNF signalling pathway. We then analysed the expression patterns of the top 10 altered genes, and found that NAC markedly stimulated HMGCS3 and LDHC expression in IPEC-J2 cells. In vitro, NAC pre-treatment significantly reduced TNF-\u03b1 and NF-\u03baB, TNF-\u03b1, IFN-\u03b3, and IL-6 expression in LPS-induced IPEC-J2 cells. NAC treatment also significantly reduced oxidative stress in LPS-induced IPEC-J2 cells and alleviated intestinal barrier function and wound healing. Thus, NAC as a feed additive can enhance livestock intestinal health by modulating intestinal inflammation, permeability, and wound healing under LPS-induced dysfunction, improving our molecular understanding of the effects of NAC on the intestine.",
"genre": "research_article",
"id": "sg:pub.10.1038/s41598-018-37296-x",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1045337",
"issn": [
"2045-2322"
],
"name": "Scientific Reports",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "9"
}
],
"name": "N-acetylcysteine modulates lipopolysaccharide-induced intestinal dysfunction",
"pagination": "1004",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"c18d42c488cb143e617af46272863c9e041d843fb2d4cfddd934c136ca7c808e"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"30700808"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101563288"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/s41598-018-37296-x"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1111778122"
]
}
],
"sameAs": [
"https://doi.org/10.1038/s41598-018-37296-x",
"https://app.dimensions.ai/details/publication/pub.1111778122"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T08:58",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000326_0000000326/records_68471_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/s41598-018-37296-x"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37296-x'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37296-x'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37296-x'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37296-x'
This table displays all metadata directly associated to this object as RDF triples.
202 TRIPLES
21 PREDICATES
67 URIs
21 LITERALS
9 BLANK NODES