Anoctamin-4 is a bona fide Ca2+-dependent non-selective cation channel View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Nadine Reichhart, Simon Schöberl, Susanne Keckeis, Ahmad S. Alfaar, Christophe Roubeix, Magdalena Cordes, Sergio Crespo-Garcia, Akvile Haeckel, Norbert Kociok, Renate Föckler, Gabriele Fels, Anja Mataruga, Robert Rauh, Vladimir M. Milenkovic, Kerstin Zühlke, Enno Klussmann, Eyk Schellenberger, Olaf Strauß

ABSTRACT

Changes in cell function occur by specific patterns of intracellular Ca2+, activating Ca2+-sensitive proteins. The anoctamin (TMEM16) protein family has Ca2+-dependent ion channel activity, which provides transmembrane ion transport, and/or Ca2+-dependent phosphatidyl-scramblase activity. Using amino acid sequence analysis combined with measurements of ion channel function, we clarified the so far unknown Ano4 function as Ca2+-dependent, non-selective monovalent cation channel; heterologous Ano4 expression in HEK293 cells elicits Ca2+ activated conductance with weak selectivity of K+ > Na+ > Li+. Endogenously expressed Ca2+-dependent cation channels in the retinal pigment epithelium were identified as Ano4 by KO mouse-derived primary RPE cells and siRNA against Ano4. Exchanging a negatively charged amino acid in the putative pore region (AA702-855) into a positive one (E775K) turns Ano4-elicited currents into Cl- currents evidencing its importance for ion selectivity. The molecular identification of Ano4 as a Ca2+-activated cation channel advances the understanding of its role in Ca2+ signaling. More... »

PAGES

2257

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-37287-y

DOI

http://dx.doi.org/10.1038/s41598-018-37287-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112224072

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30783137


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Reichhart", 
        "givenName": "Nadine", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University Hospital Regensburg", 
          "id": "https://www.grid.ac/institutes/grid.411941.8", 
          "name": [
            "Department of Gastroenterology, Endocrinology, Rheumatology and Infectious Diseases, University Hospital Regensburg, Regensburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sch\u00f6berl", 
        "givenName": "Simon", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Keckeis", 
        "givenName": "Susanne", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alfaar", 
        "givenName": "Ahmad S.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Roubeix", 
        "givenName": "Christophe", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cordes", 
        "givenName": "Magdalena", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Montreal", 
          "id": "https://www.grid.ac/institutes/grid.14848.31", 
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany", 
            "Department of Ophthalmology, Maisonneuve-Rosemont Hospital Research Centre, University of Montreal, Quebec, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Crespo-Garcia", 
        "givenName": "Sergio", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute for Radiology and Children\u2019s Radiology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Haeckel", 
        "givenName": "Akvile", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kociok", 
        "givenName": "Norbert", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University Hospital Regensburg", 
          "id": "https://www.grid.ac/institutes/grid.411941.8", 
          "name": [
            "Experimental Ophthalmology, Eye Hospital, University Medical Center Regensburg, Regensburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "F\u00f6ckler", 
        "givenName": "Renate", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fels", 
        "givenName": "Gabriele", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Forschungszentrum J\u00fclich", 
          "id": "https://www.grid.ac/institutes/grid.8385.6", 
          "name": [
            "Institute of Complex Systems, Zellul\u00e4re Biophysik (ICS-4), Forschungszentrum J\u00fclich, J\u00fclich, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mataruga", 
        "givenName": "Anja", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Erlangen-Nuremberg", 
          "id": "https://www.grid.ac/institutes/grid.5330.5", 
          "name": [
            "Institut f\u00fcr Zellul\u00e4re und Molekulare Physiologie, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), Erlangen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rauh", 
        "givenName": "Robert", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Regensburg", 
          "id": "https://www.grid.ac/institutes/grid.7727.5", 
          "name": [
            "Department of Psychiatry and Psychotherapy, Molecular Neuroscience, University of Regensburg, Regensburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Milenkovic", 
        "givenName": "Vladimir M.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine", 
          "id": "https://www.grid.ac/institutes/grid.419491.0", 
          "name": [
            "Max Delbr\u00fcck Center for Molecular Medicine Berlin (MDC), Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Z\u00fchlke", 
        "givenName": "Kerstin", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine", 
          "id": "https://www.grid.ac/institutes/grid.419491.0", 
          "name": [
            "Max Delbr\u00fcck Center for Molecular Medicine Berlin (MDC), Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klussmann", 
        "givenName": "Enno", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute for Radiology and Children\u2019s Radiology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schellenberger", 
        "givenName": "Eyk", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Experimental Ophthalmology, Department of Ophthalmology, Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, a corporate member of Freie Universit\u00e4t, Humboldt-University, the Berlin Institute of Health, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Strau\u00df", 
        "givenName": "Olaf", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1161/circresaha.112.264440", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001256924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/circresaha.112.264440", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001256924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20050113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005435053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj20050113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005435053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2012.07.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005676974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.exer.2016.12.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006418014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.visres.2005.08.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007452360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.visres.2005.08.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007452360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00424-014-1603-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008623282", 
          "https://doi.org/10.1007/s00424-014-1603-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00424-014-1603-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008623282", 
          "https://doi.org/10.1007/s00424-014-1603-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/jcs.109553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010692302"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00424-013-1305-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012897136", 
          "https://doi.org/10.1007/s00424-013-1305-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn.2821", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015754842", 
          "https://doi.org/10.1038/nn.2821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2148-10-319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019052901", 
          "https://doi.org/10.1186/1471-2148-10-319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1163518", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019512643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.201611650", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020390319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00424-014-1559-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020495731", 
          "https://doi.org/10.1007/s00424-014-1559-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2008.04.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020984956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2008.09.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021751698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m113.457937", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022054287"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.201611651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024419326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2014.09.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026180234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature13984", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027737652", 
          "https://doi.org/10.1038/nature13984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027963928", 
          "https://doi.org/10.1038/nature09583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027963928", 
          "https://doi.org/10.1038/nature09583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.00-0188com", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030496469"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07313", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033450614", 
          "https://doi.org/10.1038/nature07313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cne.20367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034521210"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.sbi.2016.05.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037604252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physrev.00039.2011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041539478"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.5546-08.2009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044263955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpcell.00228.2012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044995619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2967/jnumed.113.128108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051216478"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.26232", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085700857"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Changes in cell function occur by specific patterns of intracellular Ca2+, activating Ca2+-sensitive proteins. The anoctamin (TMEM16) protein family has Ca2+-dependent ion channel activity, which provides transmembrane ion transport, and/or Ca2+-dependent phosphatidyl-scramblase activity. Using amino acid sequence analysis combined with measurements of ion channel function, we clarified the so far unknown Ano4 function as Ca2+-dependent, non-selective monovalent cation channel; heterologous Ano4 expression in HEK293 cells elicits Ca2+ activated conductance with weak selectivity of K+\u2009>\u2009Na+\u2009>\u2009Li+. Endogenously expressed Ca2+-dependent cation channels in the retinal pigment epithelium were identified as Ano4 by KO mouse-derived primary RPE cells and siRNA against Ano4. Exchanging a negatively charged amino acid in the putative pore region (AA702-855) into a positive one (E775K) turns Ano4-elicited currents into Cl- currents evidencing its importance for ion selectivity. The molecular identification of Ano4 as a Ca2+-activated cation channel advances the understanding of its role in Ca2+ signaling.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-37287-y", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Anoctamin-4 is a bona fide Ca2+-dependent non-selective cation channel", 
    "pagination": "2257", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "45bf24aa26c51c8bf2a4733d799b5a2780d6566d600c8716da5af5a366439233"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30783137"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-37287-y"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112224072"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-37287-y", 
      "https://app.dimensions.ai/details/publication/pub.1112224072"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:42", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99843_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-37287-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37287-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37287-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37287-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37287-y'


 

This table displays all metadata directly associated to this object as RDF triples.

301 TRIPLES      21 PREDICATES      58 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-37287-y schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N7e86f058aff246098cb0c3f1bd042ebe
4 schema:citation sg:pub.10.1007/s00424-013-1305-1
5 sg:pub.10.1007/s00424-014-1559-2
6 sg:pub.10.1007/s00424-014-1603-2
7 sg:pub.10.1038/nature07313
8 sg:pub.10.1038/nature09583
9 sg:pub.10.1038/nature13984
10 sg:pub.10.1038/nn.2821
11 sg:pub.10.1186/1471-2148-10-319
12 https://doi.org/10.1002/cne.20367
13 https://doi.org/10.1016/j.ab.2008.04.036
14 https://doi.org/10.1016/j.cell.2008.09.003
15 https://doi.org/10.1016/j.cell.2012.07.036
16 https://doi.org/10.1016/j.exer.2016.12.003
17 https://doi.org/10.1016/j.jmb.2014.09.028
18 https://doi.org/10.1016/j.sbi.2016.05.020
19 https://doi.org/10.1016/j.visres.2005.08.030
20 https://doi.org/10.1042/bj20050113
21 https://doi.org/10.1074/jbc.m113.457937
22 https://doi.org/10.1085/jgp.201611650
23 https://doi.org/10.1085/jgp.201611651
24 https://doi.org/10.1096/fj.00-0188com
25 https://doi.org/10.1126/science.1163518
26 https://doi.org/10.1152/ajpcell.00228.2012
27 https://doi.org/10.1152/physrev.00039.2011
28 https://doi.org/10.1161/circresaha.112.264440
29 https://doi.org/10.1242/jcs.109553
30 https://doi.org/10.1523/jneurosci.5546-08.2009
31 https://doi.org/10.2967/jnumed.113.128108
32 https://doi.org/10.7554/elife.26232
33 schema:datePublished 2019-12
34 schema:datePublishedReg 2019-12-01
35 schema:description Changes in cell function occur by specific patterns of intracellular Ca<sup>2+</sup>, activating Ca<sup>2+</sup>-sensitive proteins. The anoctamin (TMEM16) protein family has Ca<sup>2+</sup>-dependent ion channel activity, which provides transmembrane ion transport, and/or Ca<sup>2+</sup>-dependent phosphatidyl-scramblase activity. Using amino acid sequence analysis combined with measurements of ion channel function, we clarified the so far unknown Ano4 function as Ca<sup>2+</sup>-dependent, non-selective monovalent cation channel; heterologous Ano4 expression in HEK293 cells elicits Ca<sup>2+</sup> activated conductance with weak selectivity of K<sup>+</sup> &gt; Na<sup>+</sup> &gt; Li<sup>+</sup>. Endogenously expressed Ca<sup>2+</sup>-dependent cation channels in the retinal pigment epithelium were identified as Ano4 by KO mouse-derived primary RPE cells and siRNA against Ano4. Exchanging a negatively charged amino acid in the putative pore region (AA702-855) into a positive one (E775K) turns Ano4-elicited currents into Cl<sup>-</sup> currents evidencing its importance for ion selectivity. The molecular identification of Ano4 as a Ca<sup>2+</sup>-activated cation channel advances the understanding of its role in Ca<sup>2+</sup> signaling.
36 schema:genre research_article
37 schema:inLanguage en
38 schema:isAccessibleForFree true
39 schema:isPartOf N984034f755314f4daf78d77399285a60
40 Nbc17b0d8fcf14a35bedbfc235ae43613
41 sg:journal.1045337
42 schema:name Anoctamin-4 is a bona fide Ca2+-dependent non-selective cation channel
43 schema:pagination 2257
44 schema:productId N06a36a0a207e42099aced51e1e7926dd
45 N4cd5fe09b0d84137b8b42093585cb644
46 N5ea75c3d58b8478f921498f2dbf06d2f
47 Na4b1d3a8abc0401b9040e2bdd3664374
48 Nbc0d3e26407344768acc3202b6030958
49 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112224072
50 https://doi.org/10.1038/s41598-018-37287-y
51 schema:sdDatePublished 2019-04-11T09:42
52 schema:sdLicense https://scigraph.springernature.com/explorer/license/
53 schema:sdPublisher Ndd35118379164d7ea4399affe718c208
54 schema:url https://www.nature.com/articles/s41598-018-37287-y
55 sgo:license sg:explorer/license/
56 sgo:sdDataset articles
57 rdf:type schema:ScholarlyArticle
58 N06a36a0a207e42099aced51e1e7926dd schema:name pubmed_id
59 schema:value 30783137
60 rdf:type schema:PropertyValue
61 N090e873a68a14122941304836e2e7543 rdf:first N1be9d15784c049c39955714777926d30
62 rdf:rest N4c08d09de6f748bdae0c63e5cdb9a89e
63 N0cca31e91f8c4cda9289e11865f2c1a7 schema:name Institute for Radiology and Children’s Radiology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
64 rdf:type schema:Organization
65 N0f7eebb659f34f41a411a57b7953192b schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
66 rdf:type schema:Organization
67 N1be9d15784c049c39955714777926d30 schema:affiliation N0cca31e91f8c4cda9289e11865f2c1a7
68 schema:familyName Schellenberger
69 schema:givenName Eyk
70 rdf:type schema:Person
71 N1d5a64a3f9824c65a9f575a96d8e9d6d rdf:first Nf7ee38b2ed264d029a3b8b803a7e481f
72 rdf:rest N090e873a68a14122941304836e2e7543
73 N21d157eface24b71b01b83495c0e95c1 schema:affiliation https://www.grid.ac/institutes/grid.14848.31
74 schema:familyName Crespo-Garcia
75 schema:givenName Sergio
76 rdf:type schema:Person
77 N2b48f7c936174960ba27b2cba06a10c4 schema:affiliation Nab804e19bda942d8a6d19ee78cec392c
78 schema:familyName Reichhart
79 schema:givenName Nadine
80 rdf:type schema:Person
81 N302c122ed36e476bae37bce53e72d0c1 schema:affiliation N9f1ee0cb9a8447a1a9052567ddcd7540
82 schema:familyName Haeckel
83 schema:givenName Akvile
84 rdf:type schema:Person
85 N359e668cd38a4b47b899cd3520308bd5 rdf:first N4984a4865b4b445b940b5b0b4953f49f
86 rdf:rest N40501586c18547268f15fda88942ced3
87 N3864a41a3b2e4fc2bb71694f7738ade4 schema:affiliation https://www.grid.ac/institutes/grid.411941.8
88 schema:familyName Föckler
89 schema:givenName Renate
90 rdf:type schema:Person
91 N3f089992d3304ecb9316228e1c1ed78c schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
92 rdf:type schema:Organization
93 N40501586c18547268f15fda88942ced3 rdf:first Nc24d763b88534ed9aeb255f027dc913b
94 rdf:rest Ndd05078e21d74f56b69c2e03bc9db357
95 N4984a4865b4b445b940b5b0b4953f49f schema:affiliation Nd35296395cfe4c1192fe849a43787a50
96 schema:familyName Alfaar
97 schema:givenName Ahmad S.
98 rdf:type schema:Person
99 N4c08d09de6f748bdae0c63e5cdb9a89e rdf:first N4d9b5d0f1123426b852681a0c4964f90
100 rdf:rest rdf:nil
101 N4c7b2646585f42b7876a63805a87751a rdf:first N85deacf50512446197647b071e8ec592
102 rdf:rest N8227e0153e8644f5830dde1de2494725
103 N4cd5fe09b0d84137b8b42093585cb644 schema:name nlm_unique_id
104 schema:value 101563288
105 rdf:type schema:PropertyValue
106 N4d9b5d0f1123426b852681a0c4964f90 schema:affiliation N0f7eebb659f34f41a411a57b7953192b
107 schema:familyName Strauß
108 schema:givenName Olaf
109 rdf:type schema:Person
110 N53dcc24bcf044f2d9cd176145444b04c schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
111 rdf:type schema:Organization
112 N5454fb9fa2ed4d868e4d252045ce2842 schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
113 rdf:type schema:Organization
114 N5ea75c3d58b8478f921498f2dbf06d2f schema:name readcube_id
115 schema:value 45bf24aa26c51c8bf2a4733d799b5a2780d6566d600c8716da5af5a366439233
116 rdf:type schema:PropertyValue
117 N62e393a7876d4e38a87fb9e51857b25e rdf:first Nbb62e951cb484b189d772f1cc5e903e2
118 rdf:rest Nd33392cf235646cb94fbf9ba23ff1ceb
119 N681d836f829845e4a18aa7bb2ef1b663 schema:affiliation https://www.grid.ac/institutes/grid.419491.0
120 schema:familyName Zühlke
121 schema:givenName Kerstin
122 rdf:type schema:Person
123 N6eacc29bfa804822b4e7985709882af6 schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
124 rdf:type schema:Organization
125 N6f60e9a1f3cc4d0bb5162c57cfb374ed rdf:first Nb6eba892d93e4f71ac8c613a3af2e4a0
126 rdf:rest Nbccc10bf697f40128630960eedcfd9fd
127 N72bab2668a8c4954bac6c4cdaa43ffe5 rdf:first N21d157eface24b71b01b83495c0e95c1
128 rdf:rest Naa1244fdf0804ad99030828387f48483
129 N7e86f058aff246098cb0c3f1bd042ebe rdf:first N2b48f7c936174960ba27b2cba06a10c4
130 rdf:rest N84775d0b37db468bab4b0bb9e00f6653
131 N8227e0153e8644f5830dde1de2494725 rdf:first Nb0bcd705f2f14d5d926e3a08aab35b70
132 rdf:rest N6f60e9a1f3cc4d0bb5162c57cfb374ed
133 N8337a2c196aa4c879f6a6eaa3a67f608 schema:affiliation N6eacc29bfa804822b4e7985709882af6
134 schema:familyName Keckeis
135 schema:givenName Susanne
136 rdf:type schema:Person
137 N84775d0b37db468bab4b0bb9e00f6653 rdf:first Ne513a98fcb5148399ca27ff96608c4a4
138 rdf:rest Nafb632859d254fa7b0dd5343aa3f0942
139 N85deacf50512446197647b071e8ec592 schema:affiliation https://www.grid.ac/institutes/grid.8385.6
140 schema:familyName Mataruga
141 schema:givenName Anja
142 rdf:type schema:Person
143 N984034f755314f4daf78d77399285a60 schema:volumeNumber 9
144 rdf:type schema:PublicationVolume
145 N9f1ee0cb9a8447a1a9052567ddcd7540 schema:name Institute for Radiology and Children’s Radiology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
146 rdf:type schema:Organization
147 Na4b1d3a8abc0401b9040e2bdd3664374 schema:name doi
148 schema:value 10.1038/s41598-018-37287-y
149 rdf:type schema:PropertyValue
150 Naa1244fdf0804ad99030828387f48483 rdf:first N302c122ed36e476bae37bce53e72d0c1
151 rdf:rest N62e393a7876d4e38a87fb9e51857b25e
152 Nab804e19bda942d8a6d19ee78cec392c schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
153 rdf:type schema:Organization
154 Nafb632859d254fa7b0dd5343aa3f0942 rdf:first N8337a2c196aa4c879f6a6eaa3a67f608
155 rdf:rest N359e668cd38a4b47b899cd3520308bd5
156 Nb0bcd705f2f14d5d926e3a08aab35b70 schema:affiliation https://www.grid.ac/institutes/grid.5330.5
157 schema:familyName Rauh
158 schema:givenName Robert
159 rdf:type schema:Person
160 Nb6eba892d93e4f71ac8c613a3af2e4a0 schema:affiliation https://www.grid.ac/institutes/grid.7727.5
161 schema:familyName Milenkovic
162 schema:givenName Vladimir M.
163 rdf:type schema:Person
164 Nbb0e6a71c5c0422b981a687ec959ba84 schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
165 rdf:type schema:Organization
166 Nbb62e951cb484b189d772f1cc5e903e2 schema:affiliation N5454fb9fa2ed4d868e4d252045ce2842
167 schema:familyName Kociok
168 schema:givenName Norbert
169 rdf:type schema:Person
170 Nbc0d3e26407344768acc3202b6030958 schema:name dimensions_id
171 schema:value pub.1112224072
172 rdf:type schema:PropertyValue
173 Nbc17b0d8fcf14a35bedbfc235ae43613 schema:issueNumber 1
174 rdf:type schema:PublicationIssue
175 Nbccc10bf697f40128630960eedcfd9fd rdf:first N681d836f829845e4a18aa7bb2ef1b663
176 rdf:rest N1d5a64a3f9824c65a9f575a96d8e9d6d
177 Nc24d763b88534ed9aeb255f027dc913b schema:affiliation Nbb0e6a71c5c0422b981a687ec959ba84
178 schema:familyName Roubeix
179 schema:givenName Christophe
180 rdf:type schema:Person
181 Nd2015c236c034e2f99cfa0461ea3a1ee schema:affiliation N53dcc24bcf044f2d9cd176145444b04c
182 schema:familyName Cordes
183 schema:givenName Magdalena
184 rdf:type schema:Person
185 Nd33392cf235646cb94fbf9ba23ff1ceb rdf:first N3864a41a3b2e4fc2bb71694f7738ade4
186 rdf:rest Nd85fe45900ce45eabf2157a70e4ef32b
187 Nd35296395cfe4c1192fe849a43787a50 schema:name Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
188 rdf:type schema:Organization
189 Nd85fe45900ce45eabf2157a70e4ef32b rdf:first Nf5b01d4eb5bf4ad582409a755f89f321
190 rdf:rest N4c7b2646585f42b7876a63805a87751a
191 Ndd05078e21d74f56b69c2e03bc9db357 rdf:first Nd2015c236c034e2f99cfa0461ea3a1ee
192 rdf:rest N72bab2668a8c4954bac6c4cdaa43ffe5
193 Ndd35118379164d7ea4399affe718c208 schema:name Springer Nature - SN SciGraph project
194 rdf:type schema:Organization
195 Ne513a98fcb5148399ca27ff96608c4a4 schema:affiliation https://www.grid.ac/institutes/grid.411941.8
196 schema:familyName Schöberl
197 schema:givenName Simon
198 rdf:type schema:Person
199 Nf5b01d4eb5bf4ad582409a755f89f321 schema:affiliation N3f089992d3304ecb9316228e1c1ed78c
200 schema:familyName Fels
201 schema:givenName Gabriele
202 rdf:type schema:Person
203 Nf7ee38b2ed264d029a3b8b803a7e481f schema:affiliation https://www.grid.ac/institutes/grid.419491.0
204 schema:familyName Klussmann
205 schema:givenName Enno
206 rdf:type schema:Person
207 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
208 schema:name Biological Sciences
209 rdf:type schema:DefinedTerm
210 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
211 schema:name Biochemistry and Cell Biology
212 rdf:type schema:DefinedTerm
213 sg:journal.1045337 schema:issn 2045-2322
214 schema:name Scientific Reports
215 rdf:type schema:Periodical
216 sg:pub.10.1007/s00424-013-1305-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012897136
217 https://doi.org/10.1007/s00424-013-1305-1
218 rdf:type schema:CreativeWork
219 sg:pub.10.1007/s00424-014-1559-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020495731
220 https://doi.org/10.1007/s00424-014-1559-2
221 rdf:type schema:CreativeWork
222 sg:pub.10.1007/s00424-014-1603-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008623282
223 https://doi.org/10.1007/s00424-014-1603-2
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/nature07313 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033450614
226 https://doi.org/10.1038/nature07313
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/nature09583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027963928
229 https://doi.org/10.1038/nature09583
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/nature13984 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027737652
232 https://doi.org/10.1038/nature13984
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nn.2821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015754842
235 https://doi.org/10.1038/nn.2821
236 rdf:type schema:CreativeWork
237 sg:pub.10.1186/1471-2148-10-319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019052901
238 https://doi.org/10.1186/1471-2148-10-319
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1002/cne.20367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034521210
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.ab.2008.04.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020984956
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.cell.2008.09.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021751698
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.cell.2012.07.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005676974
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/j.exer.2016.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006418014
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/j.jmb.2014.09.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026180234
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/j.sbi.2016.05.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037604252
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/j.visres.2005.08.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007452360
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1042/bj20050113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005435053
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1074/jbc.m113.457937 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022054287
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1085/jgp.201611650 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020390319
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1085/jgp.201611651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024419326
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1096/fj.00-0188com schema:sameAs https://app.dimensions.ai/details/publication/pub.1030496469
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1126/science.1163518 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019512643
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1152/ajpcell.00228.2012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044995619
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1152/physrev.00039.2011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041539478
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1161/circresaha.112.264440 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001256924
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1242/jcs.109553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010692302
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1523/jneurosci.5546-08.2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044263955
277 rdf:type schema:CreativeWork
278 https://doi.org/10.2967/jnumed.113.128108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051216478
279 rdf:type schema:CreativeWork
280 https://doi.org/10.7554/elife.26232 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085700857
281 rdf:type schema:CreativeWork
282 https://www.grid.ac/institutes/grid.14848.31 schema:alternateName University of Montreal
283 schema:name Department of Ophthalmology, Maisonneuve-Rosemont Hospital Research Centre, University of Montreal, Quebec, Canada
284 Experimental Ophthalmology, Department of Ophthalmology, Charité – Universitätsmedizin Berlin, a corporate member of Freie Universität, Humboldt-University, the Berlin Institute of Health, Berlin, Germany
285 rdf:type schema:Organization
286 https://www.grid.ac/institutes/grid.411941.8 schema:alternateName University Hospital Regensburg
287 schema:name Department of Gastroenterology, Endocrinology, Rheumatology and Infectious Diseases, University Hospital Regensburg, Regensburg, Germany
288 Experimental Ophthalmology, Eye Hospital, University Medical Center Regensburg, Regensburg, Germany
289 rdf:type schema:Organization
290 https://www.grid.ac/institutes/grid.419491.0 schema:alternateName Max Delbrück Center for Molecular Medicine
291 schema:name Max Delbrück Center for Molecular Medicine Berlin (MDC), Berlin, Germany
292 rdf:type schema:Organization
293 https://www.grid.ac/institutes/grid.5330.5 schema:alternateName University of Erlangen-Nuremberg
294 schema:name Institut für Zelluläre und Molekulare Physiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany
295 rdf:type schema:Organization
296 https://www.grid.ac/institutes/grid.7727.5 schema:alternateName University of Regensburg
297 schema:name Department of Psychiatry and Psychotherapy, Molecular Neuroscience, University of Regensburg, Regensburg, Germany
298 rdf:type schema:Organization
299 https://www.grid.ac/institutes/grid.8385.6 schema:alternateName Forschungszentrum Jülich
300 schema:name Institute of Complex Systems, Zelluläre Biophysik (ICS-4), Forschungszentrum Jülich, Jülich, Germany
301 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...