Directed evolution of super-secreted variants from phage-displayed human Interleukin-2 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Gertrudis Rojas, Tania Carmenate, Julio Felipe Santo-Tomás, Pedro A. Valiente, Marlies Becker, Annia Pérez-Riverón, Yaima Tundidor, Yaquelín Ortiz, Jorge Fernandez de Cossio-Diaz, Luis Graça, Stefan Dübel, Kalet León

ABSTRACT

Selection from a phage display library derived from human Interleukin-2 (IL-2) yielded mutated variants with greatly enhanced display levels of the functional cytokine on filamentous phages. Introduction of a single amino acid replacement selected that way (K35E) increased the secretion levels of IL-2-containing fusion proteins from human transfected host cells up to 20-fold. Super-secreted (K35E) IL-2/Fc is biologically active in vitro and in vivo, has anti-tumor activity and exhibits a remarkable reduction in its aggregation propensity- the major manufacturability issue limiting IL-2 usefulness up to now. Improvement of secretion was also shown for a panel of IL-2-engineered variants with altered receptor binding properties, including a selective agonist and a super agonist that kept their unique properties. Our findings will improve developability of the growing family of IL-2-derived immunotherapeutic agents and could have a broader impact on the engineering of structurally related four-alpha-helix bundle cytokines. More... »

PAGES

800

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-37280-5

DOI

http://dx.doi.org/10.1038/s41598-018-37280-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111673707

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30692603


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rojas", 
        "givenName": "Gertrudis", 
        "id": "sg:person.01242451603.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242451603.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Carmenate", 
        "givenName": "Tania", 
        "id": "sg:person.01265653062.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265653062.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Santo-Tom\u00e1s", 
        "givenName": "Julio Felipe", 
        "id": "sg:person.01260363360.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260363360.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Biology Faculty, La Habana University, 25 e/ I y J, Vedado, CP 10400, Plaza, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Valiente", 
        "givenName": "Pedro A.", 
        "id": "sg:person.0721023723.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721023723.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Braunschweig University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6738.a", 
          "name": [
            "Technische Universit\u00e4t Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics, Department of Biotechnology, Spielmannstra\u00dfe 7, 38106, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Becker", 
        "givenName": "Marlies", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "P\u00e9rez-River\u00f3n", 
        "givenName": "Annia", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tundidor", 
        "givenName": "Yaima", 
        "id": "sg:person.01343021476.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343021476.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ortiz", 
        "givenName": "Yaquel\u00edn", 
        "id": "sg:person.012746117726.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012746117726.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Cossio-Diaz", 
        "givenName": "Jorge Fernandez", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Lisbon", 
          "id": "https://www.grid.ac/institutes/grid.9983.b", 
          "name": [
            "Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisbon, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gra\u00e7a", 
        "givenName": "Luis", 
        "id": "sg:person.013624662120.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013624662120.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Braunschweig University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.6738.a", 
          "name": [
            "Technische Universit\u00e4t Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics, Department of Biotechnology, Spielmannstra\u00dfe 7, 38106, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "D\u00fcbel", 
        "givenName": "Stefan", 
        "id": "sg:person.01102431427.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102431427.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Center of Molecular Immunology (Cuba)", 
          "id": "https://www.grid.ac/institutes/grid.417645.5", 
          "name": [
            "Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Le\u00f3n", 
        "givenName": "Kalet", 
        "id": "sg:person.0771413263.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771413263.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/nar/11.13.4307", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001340439"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jim.2004.07.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001904459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2010.01.051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002531192"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/gzg111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003528062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bej.2005.03.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003926304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.immuni.2015.04.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004698812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1108188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005731628"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/gzq107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009707719"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jmr.2440", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010759834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jps.24243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012184973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/msb.2013.47", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012785380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/msb.2013.47", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012785380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-10-2921", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013292733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1472-6750-13-52", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014598611", 
          "https://doi.org/10.1186/1472-6750-13-52"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1416159112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018055199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-065x.1980.tb00327.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018538522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02678213", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023519886", 
          "https://doi.org/10.1007/bf02678213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02678213", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023519886", 
          "https://doi.org/10.1007/bf02678213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.2.488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026991187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ccell.2015.03.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027600761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr960065d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028752028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr960065d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028752028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0511161103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029644239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e3181b528da", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032204498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e3181b528da", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032204498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e3181b528da", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032204498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1202866109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033799431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1122927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035511086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature10975", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036269489", 
          "https://doi.org/10.1038/nature10975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-15-1631", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037545828"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep03333", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037973088", 
          "https://doi.org/10.1038/srep03333"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.celrep.2012.09.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038181300"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/cyto.1997.0228", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038294910"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bpj.2012.01.060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038416320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst20120160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039950219"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst20120160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039950219"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1435", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040502284", 
          "https://doi.org/10.1038/nri1435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1435", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040502284", 
          "https://doi.org/10.1038/nri1435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2450085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042570329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj2450085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042570329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.imbio.2012.02.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043166390"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/2162402x.2016.1277306", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044001523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10930-012-9429-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044160972", 
          "https://doi.org/10.1007/s10930-012-9429-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s2213-8587(13)70113-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044427513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2006.10.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046338827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1117893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046426912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cncr.23552", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047321867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1380-2933(97)00012-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047525655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja071641y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047577486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja071641y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047577486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci46266", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048164351"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abbi.1996.9853", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049391171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ct700301q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055425839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ct700301q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055425839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp982007x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056127812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp982007x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056127812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-139-10-2465", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060368539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/1568026611212220003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069194514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/mabs.27224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072305713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/mabs.28395", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072305760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077890429", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.1201895", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078730412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079538017", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncomms15373", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1085393505", 
          "https://doi.org/10.1038/ncomms15373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4939-7447-4_30", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1092578438", 
          "https://doi.org/10.1007/978-1-4939-7447-4_30"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.1700433", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103278762"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.1700433", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1103278762"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-12", 
    "datePublishedReg": "2019-12-01", 
    "description": "Selection from a phage display library derived from human Interleukin-2 (IL-2) yielded mutated variants with greatly enhanced display levels of the functional cytokine on filamentous phages. Introduction of a single amino acid replacement selected that way (K35E) increased the secretion levels of IL-2-containing fusion proteins from human transfected host cells up to 20-fold. Super-secreted (K35E) IL-2/Fc is biologically active in vitro and in vivo, has anti-tumor activity and exhibits a remarkable reduction in its aggregation propensity- the major manufacturability issue limiting IL-2 usefulness up to now. Improvement of secretion was also shown for a panel of IL-2-engineered variants with altered receptor binding properties, including a selective agonist and a super agonist that kept their unique properties. Our findings will improve developability of the growing family of IL-2-derived immunotherapeutic agents and could have a broader impact on the engineering of structurally related four-alpha-helix bundle cytokines.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/s41598-018-37280-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1045337", 
        "issn": [
          "2045-2322"
        ], 
        "name": "Scientific Reports", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "9"
      }
    ], 
    "name": "Directed evolution of super-secreted variants from phage-displayed human Interleukin-2", 
    "pagination": "800", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "856ea1f4c7ae62e1b796441482622185b075e07609ce0018b55318aabab5eae5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30692603"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101563288"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/s41598-018-37280-5"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1111673707"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/s41598-018-37280-5", 
      "https://app.dimensions.ai/details/publication/pub.1111673707"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T08:58", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000326_0000000326/records_68454_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/s41598-018-37280-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37280-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37280-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37280-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-37280-5'


 

This table displays all metadata directly associated to this object as RDF triples.

321 TRIPLES      21 PREDICATES      84 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/s41598-018-37280-5 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N27134955d7294d0a9e58b46242dec557
4 schema:citation sg:pub.10.1007/978-1-4939-7447-4_30
5 sg:pub.10.1007/bf02678213
6 sg:pub.10.1007/s10930-012-9429-2
7 sg:pub.10.1038/nature10975
8 sg:pub.10.1038/ncomms15373
9 sg:pub.10.1038/nri1435
10 sg:pub.10.1038/srep03333
11 sg:pub.10.1186/1472-6750-13-52
12 https://app.dimensions.ai/details/publication/pub.1077890429
13 https://app.dimensions.ai/details/publication/pub.1079538017
14 https://doi.org/10.1002/cncr.23552
15 https://doi.org/10.1002/jmr.2440
16 https://doi.org/10.1002/jps.24243
17 https://doi.org/10.1006/abbi.1996.9853
18 https://doi.org/10.1006/cyto.1997.0228
19 https://doi.org/10.1016/j.bej.2005.03.002
20 https://doi.org/10.1016/j.bpj.2010.01.051
21 https://doi.org/10.1016/j.bpj.2012.01.060
22 https://doi.org/10.1016/j.ccell.2015.03.004
23 https://doi.org/10.1016/j.celrep.2012.09.036
24 https://doi.org/10.1016/j.imbio.2012.02.009
25 https://doi.org/10.1016/j.immuni.2015.04.018
26 https://doi.org/10.1016/j.jim.2004.07.002
27 https://doi.org/10.1016/j.jmb.2006.10.026
28 https://doi.org/10.1016/s1380-2933(97)00012-2
29 https://doi.org/10.1016/s2213-8587(13)70113-x
30 https://doi.org/10.1021/cr960065d
31 https://doi.org/10.1021/ct700301q
32 https://doi.org/10.1021/ja071641y
33 https://doi.org/10.1021/jp982007x
34 https://doi.org/10.1038/msb.2013.47
35 https://doi.org/10.1042/bj2450085
36 https://doi.org/10.1042/bst20120160
37 https://doi.org/10.1056/nejmoa1108188
38 https://doi.org/10.1073/pnas.0511161103
39 https://doi.org/10.1073/pnas.1202866109
40 https://doi.org/10.1073/pnas.1416159112
41 https://doi.org/10.1073/pnas.82.2.488
42 https://doi.org/10.1080/2162402x.2016.1277306
43 https://doi.org/10.1093/nar/11.13.4307
44 https://doi.org/10.1093/protein/gzg111
45 https://doi.org/10.1093/protein/gzq107
46 https://doi.org/10.1097/cji.0b013e3181b528da
47 https://doi.org/10.1099/00221287-139-10-2465
48 https://doi.org/10.1111/j.1600-065x.1980.tb00327.x
49 https://doi.org/10.1126/science.1117893
50 https://doi.org/10.1126/science.1122927
51 https://doi.org/10.1158/1078-0432.ccr-10-2921
52 https://doi.org/10.1158/1078-0432.ccr-15-1631
53 https://doi.org/10.1172/jci46266
54 https://doi.org/10.2174/1568026611212220003
55 https://doi.org/10.4049/jimmunol.1201895
56 https://doi.org/10.4049/jimmunol.1700433
57 https://doi.org/10.4161/mabs.27224
58 https://doi.org/10.4161/mabs.28395
59 schema:datePublished 2019-12
60 schema:datePublishedReg 2019-12-01
61 schema:description Selection from a phage display library derived from human Interleukin-2 (IL-2) yielded mutated variants with greatly enhanced display levels of the functional cytokine on filamentous phages. Introduction of a single amino acid replacement selected that way (K35E) increased the secretion levels of IL-2-containing fusion proteins from human transfected host cells up to 20-fold. Super-secreted (K35E) IL-2/Fc is biologically active in vitro and in vivo, has anti-tumor activity and exhibits a remarkable reduction in its aggregation propensity- the major manufacturability issue limiting IL-2 usefulness up to now. Improvement of secretion was also shown for a panel of IL-2-engineered variants with altered receptor binding properties, including a selective agonist and a super agonist that kept their unique properties. Our findings will improve developability of the growing family of IL-2-derived immunotherapeutic agents and could have a broader impact on the engineering of structurally related four-alpha-helix bundle cytokines.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree true
65 schema:isPartOf N99ceba1f8e774a1abf72e0c836a4ab8e
66 Nec98b35dabf14bb9a978fbc8a854c79c
67 sg:journal.1045337
68 schema:name Directed evolution of super-secreted variants from phage-displayed human Interleukin-2
69 schema:pagination 800
70 schema:productId N27dc8334bd4c4798bf46a63d8a7ba5b4
71 N6b19ebae6d464ed08d47208d266986a9
72 N94bc23aee8f443b8a7bc18cce1d55a6c
73 Nb59c86c1daab490bab05cc274a7d1b01
74 Nda43412d85994bb1848e616dd871ddf7
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111673707
76 https://doi.org/10.1038/s41598-018-37280-5
77 schema:sdDatePublished 2019-04-11T08:58
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher N2537d36b54b6478f9b76a578bca224dd
80 schema:url https://www.nature.com/articles/s41598-018-37280-5
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N17d4d58680174528babba0dd42de7ad2 schema:affiliation https://www.grid.ac/institutes/grid.417645.5
85 schema:familyName Pérez-Riverón
86 schema:givenName Annia
87 rdf:type schema:Person
88 N252d2a65417641d0b949e01b2818367d rdf:first Nbd8e000512c14908a322e8e626ac687d
89 rdf:rest Nb657bed262a746e1b1bb913a82c166ca
90 N2537d36b54b6478f9b76a578bca224dd schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 N27134955d7294d0a9e58b46242dec557 rdf:first sg:person.01242451603.40
93 rdf:rest N9c864771275942ed911ceda53e15ebac
94 N27dc8334bd4c4798bf46a63d8a7ba5b4 schema:name dimensions_id
95 schema:value pub.1111673707
96 rdf:type schema:PropertyValue
97 N3a0006b326514834b22aecf95fb9502a rdf:first sg:person.0721023723.23
98 rdf:rest N252d2a65417641d0b949e01b2818367d
99 N6b19ebae6d464ed08d47208d266986a9 schema:name pubmed_id
100 schema:value 30692603
101 rdf:type schema:PropertyValue
102 N75f5b702b58c424d97999e5fa9aeb1b6 rdf:first Ndb39590efc524b19941967699346d59e
103 rdf:rest N7b17095bd4dc49a5b5c33d701f491c45
104 N7b17095bd4dc49a5b5c33d701f491c45 rdf:first sg:person.013624662120.44
105 rdf:rest Ndeb8ba042301489e8c104a1154202ae6
106 N83f1ae5cc5ec44f8854a7977843e36ad schema:name Biology Faculty, La Habana University, 25 e/ I y J, Vedado, CP 10400, Plaza, La Habana, Cuba
107 rdf:type schema:Organization
108 N94bc23aee8f443b8a7bc18cce1d55a6c schema:name nlm_unique_id
109 schema:value 101563288
110 rdf:type schema:PropertyValue
111 N99ceba1f8e774a1abf72e0c836a4ab8e schema:issueNumber 1
112 rdf:type schema:PublicationIssue
113 N9c864771275942ed911ceda53e15ebac rdf:first sg:person.01265653062.00
114 rdf:rest Nd80c138a90794cfe988c77395a46f970
115 Na0649987af194ec3b53eb4bbe4c78df5 rdf:first sg:person.01343021476.65
116 rdf:rest Nd76402d97a22452babbfdc139ab74e9c
117 Nb59c86c1daab490bab05cc274a7d1b01 schema:name readcube_id
118 schema:value 856ea1f4c7ae62e1b796441482622185b075e07609ce0018b55318aabab5eae5
119 rdf:type schema:PropertyValue
120 Nb657bed262a746e1b1bb913a82c166ca rdf:first N17d4d58680174528babba0dd42de7ad2
121 rdf:rest Na0649987af194ec3b53eb4bbe4c78df5
122 Nbd8e000512c14908a322e8e626ac687d schema:affiliation https://www.grid.ac/institutes/grid.6738.a
123 schema:familyName Becker
124 schema:givenName Marlies
125 rdf:type schema:Person
126 Nd76402d97a22452babbfdc139ab74e9c rdf:first sg:person.012746117726.44
127 rdf:rest N75f5b702b58c424d97999e5fa9aeb1b6
128 Nd80c138a90794cfe988c77395a46f970 rdf:first sg:person.01260363360.53
129 rdf:rest N3a0006b326514834b22aecf95fb9502a
130 Nda43412d85994bb1848e616dd871ddf7 schema:name doi
131 schema:value 10.1038/s41598-018-37280-5
132 rdf:type schema:PropertyValue
133 Ndb39590efc524b19941967699346d59e schema:affiliation https://www.grid.ac/institutes/grid.417645.5
134 schema:familyName de Cossio-Diaz
135 schema:givenName Jorge Fernandez
136 rdf:type schema:Person
137 Ndeb8ba042301489e8c104a1154202ae6 rdf:first sg:person.01102431427.44
138 rdf:rest Ne9fe4b4864994536b8e123fa7914d0e1
139 Ne9fe4b4864994536b8e123fa7914d0e1 rdf:first sg:person.0771413263.29
140 rdf:rest rdf:nil
141 Nec98b35dabf14bb9a978fbc8a854c79c schema:volumeNumber 9
142 rdf:type schema:PublicationVolume
143 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
144 schema:name Biological Sciences
145 rdf:type schema:DefinedTerm
146 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
147 schema:name Biochemistry and Cell Biology
148 rdf:type schema:DefinedTerm
149 sg:journal.1045337 schema:issn 2045-2322
150 schema:name Scientific Reports
151 rdf:type schema:Periodical
152 sg:person.01102431427.44 schema:affiliation https://www.grid.ac/institutes/grid.6738.a
153 schema:familyName Dübel
154 schema:givenName Stefan
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102431427.44
156 rdf:type schema:Person
157 sg:person.01242451603.40 schema:affiliation https://www.grid.ac/institutes/grid.417645.5
158 schema:familyName Rojas
159 schema:givenName Gertrudis
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242451603.40
161 rdf:type schema:Person
162 sg:person.01260363360.53 schema:affiliation https://www.grid.ac/institutes/grid.417645.5
163 schema:familyName Santo-Tomás
164 schema:givenName Julio Felipe
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260363360.53
166 rdf:type schema:Person
167 sg:person.01265653062.00 schema:affiliation https://www.grid.ac/institutes/grid.417645.5
168 schema:familyName Carmenate
169 schema:givenName Tania
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265653062.00
171 rdf:type schema:Person
172 sg:person.012746117726.44 schema:affiliation https://www.grid.ac/institutes/grid.417645.5
173 schema:familyName Ortiz
174 schema:givenName Yaquelín
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012746117726.44
176 rdf:type schema:Person
177 sg:person.01343021476.65 schema:affiliation https://www.grid.ac/institutes/grid.417645.5
178 schema:familyName Tundidor
179 schema:givenName Yaima
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343021476.65
181 rdf:type schema:Person
182 sg:person.013624662120.44 schema:affiliation https://www.grid.ac/institutes/grid.9983.b
183 schema:familyName Graça
184 schema:givenName Luis
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013624662120.44
186 rdf:type schema:Person
187 sg:person.0721023723.23 schema:affiliation N83f1ae5cc5ec44f8854a7977843e36ad
188 schema:familyName Valiente
189 schema:givenName Pedro A.
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721023723.23
191 rdf:type schema:Person
192 sg:person.0771413263.29 schema:affiliation https://www.grid.ac/institutes/grid.417645.5
193 schema:familyName León
194 schema:givenName Kalet
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771413263.29
196 rdf:type schema:Person
197 sg:pub.10.1007/978-1-4939-7447-4_30 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092578438
198 https://doi.org/10.1007/978-1-4939-7447-4_30
199 rdf:type schema:CreativeWork
200 sg:pub.10.1007/bf02678213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023519886
201 https://doi.org/10.1007/bf02678213
202 rdf:type schema:CreativeWork
203 sg:pub.10.1007/s10930-012-9429-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044160972
204 https://doi.org/10.1007/s10930-012-9429-2
205 rdf:type schema:CreativeWork
206 sg:pub.10.1038/nature10975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036269489
207 https://doi.org/10.1038/nature10975
208 rdf:type schema:CreativeWork
209 sg:pub.10.1038/ncomms15373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085393505
210 https://doi.org/10.1038/ncomms15373
211 rdf:type schema:CreativeWork
212 sg:pub.10.1038/nri1435 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040502284
213 https://doi.org/10.1038/nri1435
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/srep03333 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037973088
216 https://doi.org/10.1038/srep03333
217 rdf:type schema:CreativeWork
218 sg:pub.10.1186/1472-6750-13-52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014598611
219 https://doi.org/10.1186/1472-6750-13-52
220 rdf:type schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1077890429 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1079538017 schema:CreativeWork
223 https://doi.org/10.1002/cncr.23552 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047321867
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1002/jmr.2440 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010759834
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1002/jps.24243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012184973
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1006/abbi.1996.9853 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049391171
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1006/cyto.1997.0228 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038294910
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/j.bej.2005.03.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003926304
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/j.bpj.2010.01.051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002531192
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/j.bpj.2012.01.060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038416320
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/j.ccell.2015.03.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027600761
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/j.celrep.2012.09.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038181300
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.imbio.2012.02.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043166390
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.immuni.2015.04.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004698812
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1016/j.jim.2004.07.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001904459
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/j.jmb.2006.10.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046338827
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/s1380-2933(97)00012-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047525655
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/s2213-8587(13)70113-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044427513
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1021/cr960065d schema:sameAs https://app.dimensions.ai/details/publication/pub.1028752028
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1021/ct700301q schema:sameAs https://app.dimensions.ai/details/publication/pub.1055425839
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1021/ja071641y schema:sameAs https://app.dimensions.ai/details/publication/pub.1047577486
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1021/jp982007x schema:sameAs https://app.dimensions.ai/details/publication/pub.1056127812
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1038/msb.2013.47 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012785380
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1042/bj2450085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042570329
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1042/bst20120160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039950219
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1056/nejmoa1108188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005731628
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1073/pnas.0511161103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029644239
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1073/pnas.1202866109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033799431
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1073/pnas.1416159112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018055199
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1073/pnas.82.2.488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026991187
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1080/2162402x.2016.1277306 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044001523
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1093/nar/11.13.4307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001340439
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1093/protein/gzg111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003528062
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1093/protein/gzq107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009707719
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1097/cji.0b013e3181b528da schema:sameAs https://app.dimensions.ai/details/publication/pub.1032204498
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1099/00221287-139-10-2465 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060368539
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1111/j.1600-065x.1980.tb00327.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018538522
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1126/science.1117893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046426912
294 rdf:type schema:CreativeWork
295 https://doi.org/10.1126/science.1122927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035511086
296 rdf:type schema:CreativeWork
297 https://doi.org/10.1158/1078-0432.ccr-10-2921 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013292733
298 rdf:type schema:CreativeWork
299 https://doi.org/10.1158/1078-0432.ccr-15-1631 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037545828
300 rdf:type schema:CreativeWork
301 https://doi.org/10.1172/jci46266 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048164351
302 rdf:type schema:CreativeWork
303 https://doi.org/10.2174/1568026611212220003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069194514
304 rdf:type schema:CreativeWork
305 https://doi.org/10.4049/jimmunol.1201895 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078730412
306 rdf:type schema:CreativeWork
307 https://doi.org/10.4049/jimmunol.1700433 schema:sameAs https://app.dimensions.ai/details/publication/pub.1103278762
308 rdf:type schema:CreativeWork
309 https://doi.org/10.4161/mabs.27224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072305713
310 rdf:type schema:CreativeWork
311 https://doi.org/10.4161/mabs.28395 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072305760
312 rdf:type schema:CreativeWork
313 https://www.grid.ac/institutes/grid.417645.5 schema:alternateName Center of Molecular Immunology (Cuba)
314 schema:name Center of Molecular Immunology, calle 216 esq 15, apartado 16040, Atabey, CP 11300, Playa, La Habana, Cuba
315 rdf:type schema:Organization
316 https://www.grid.ac/institutes/grid.6738.a schema:alternateName Braunschweig University of Technology
317 schema:name Technische Universität Braunschweig, Institute of Biochemistry, Biotechnology and Bioinformatics, Department of Biotechnology, Spielmannstraße 7, 38106, Braunschweig, Germany
318 rdf:type schema:Organization
319 https://www.grid.ac/institutes/grid.9983.b schema:alternateName University of Lisbon
320 schema:name Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisbon, Portugal
321 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...