High-resolution experimental and computational electrophysiology reveals weak β-lactam binding events in the porin PorB View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Annika Bartsch, Salomé Llabrés, Florian Pein, Christof Kattner, Markus Schön, Manuel Diehn, Mikio Tanabe, Axel Munk, Ulrich Zachariae, Claudia Steinem

ABSTRACT

The permeation of most antibiotics through the outer membrane of Gram-negative bacteria occurs through porin channels. To design drugs with increased activity against Gram-negative bacteria in the face of the antibiotic resistance crisis, the strict constraints on the physicochemical properties of the permeants imposed by these channels must be better understood. Here we show that a combination of high-resolution electrophysiology, new noise-filtering analysis protocols and atomistic biomolecular simulations reveals weak binding events between the β-lactam antibiotic ampicillin and the porin PorB from the pathogenic bacterium Neisseria meningitidis. In particular, an asymmetry often seen in the electrophysiological characteristics of ligand-bound channels is utilised to characterise the binding site and molecular interactions in detail, based on the principles of electro-osmotic flow through the channel. Our results provide a rationale for the determinants that govern the binding and permeation of zwitterionic antibiotics in porin channels. More... »

PAGES

1264

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-37066-9

DOI

http://dx.doi.org/10.1038/s41598-018-37066-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111915632

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30718567


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