Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Faisal Minshawi, Mike R. H. White, Werner Muller, Neil Humphreys, Dean Jackson, Barry J. Campbell, Antony Adamson, Stamatia Papoutsopoulou

ABSTRACT

Tumour necrosis factor (TNF) is a key cytokine during inflammatory responses and its dysregulation is detrimental in many inflammatory diseases, such as rheumatoid arthritis and inflammatory bowel disease. Here, we used a bacterial artificial chromosome (BAC) construct that expresses luciferase under the control of the human TNF locus to generate a novel transgenic mouse, the hTNF.LucBAC strain. In vitro stimulation of hTNF.LucBAC cells of different origin revealed a cell specific response to stimuli demonstrating the integrated construct's ability as a proxy for inflammatory gene response. Lipopolysaccharide was the most potent luciferase inducer in macrophages, while TNF was a strong activator in intestinal organoids. Lipopolysaccharide-induced luciferase activity in macrophages was downregulated by inhibitors of NF-κB pathway, as well as by Interleukin-10, a known anti-inflammatory cytokine. Moreover, the transgene-dependent luciferase activity showed a positive correlation to the endogenous murine soluble TNF secreted to the culture medium. In conclusion, the hTNF.LucBAC strain is a valuable tool for studying and screening molecules that target TNF synthesis and will allow further functional studies of the regulatory elements of the TNF locus. More... »

PAGES

193

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/s41598-018-36969-x

DOI

http://dx.doi.org/10.1038/s41598-018-36969-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1111390324

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30655563


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36969-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36969-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36969-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36969-x'


 

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291 rdf:type schema:Organization
292 https://www.grid.ac/institutes/grid.412832.e schema:alternateName Umm al-Qura University
293 schema:name Department of Laboratory Medicine, Faculty of Applied Medical Science, Umm Al-Qura University, Makkah, Saudi Arabia
294 Faculty of Biology, Medicine and Health, School of Biological Sciences, M13 9PT, Manchester, United Kingdom
295 rdf:type schema:Organization
 




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