Identification of a Xist silencing domain by Tiling CRISPR View Full Text


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Article Info

DATE

2019-12

AUTHORS

Yang Wang, Yang Zhong, Yingyao Zhou, Olga Tanaseichuk, Zhizhong Li, Jing Crystal Zhao

ABSTRACT

Despite essential roles played by long noncoding RNAs (lncRNAs) in development and disease, methods to determine lncRNA cis-elements are lacking. Here, we developed a screening method named "Tiling CRISPR" to identify lncRNA functional domains. Using this approach, we identified Xist A-Repeats as the silencing domain, an observation in agreement with published work, suggesting Tiling CRISPR feasibility. Mechanistic analysis suggested a novel function for Xist A-repeats in promoting Xist transcription. Overall, our method allows mapping of lncRNA functional domains in an unbiased and potentially high-throughput manner to facilitate the understanding of lncRNA functions. More... »

PAGES

2408

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-36750-0

    DOI

    http://dx.doi.org/10.1038/s41598-018-36750-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1112221792

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30787302


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    JSON-LD is a popular format for linked data which is fully compatible with JSON.

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    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36750-0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36750-0'

    RDF/XML is a standard XML format for linked data.

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