Characterization of sulfur-compound metabolism underlying wax-ester fermentation in Euglena gracilis View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12

AUTHORS

Koji Yamada, Tomoaki Nitta, Kohei Atsuji, Maeka Shiroyama, Komaki Inoue, Chieko Higuchi, Nobuko Nitta, Satoshi Oshiro, Keiichi Mochida, Osamu Iwata, Iwao Ohtsu, Kengo Suzuki

ABSTRACT

Euglena gracilis is a microalga, which has been used as a model organism for decades. Recent technological advances have enabled mass cultivation of this species for industrial applications such as feedstock in nutritional foods and cosmetics. E. gracilis degrades its storage polysaccharide (paramylon) under hypoxic conditions for energy acquisition by an oxygen-independent process and accumulates high amount of wax-ester as a by-product. Using this sequence of reactions referred to as wax-ester fermentation, E. gracilis is studied for its application in biofuel production. Although the wax-ester production pathway is well characterized, little is known regarding the biochemical reactions underlying the main metabolic route, especially, the existence of an unknown sulfur-compound metabolism implied by the nasty odor generation accompanying the wax-ester fermentation. In this study, we show sulfur-metabolomics of E. gracilis in aerobic and hypoxic conditions, to reveal the biochemical reactions that occur during wax-ester synthesis. Our results helped us in identifying hydrogen sulfide (H2S) as the nasty odor-producing component in wax-ester fermentation. In addition, the results indicate that glutathione and protein degrades during hypoxia, whereas cysteine, methionine, and their metabolites increase in the cells. This indicates that this shift of abundance in sulfur compounds is the cause of H2S synthesis. More... »

PAGES

853

References to SciGraph publications

  • 1952-01. Growth and photosynthetic characteristics of euglena gracilis in ARCHIVES OF MICROBIOLOGY
  • 2016-09. Efficient selective breeding of live oil-rich Euglena gracilis with fluorescence-activated cell sorting in SCIENTIFIC REPORTS
  • 2017. Large-Scale Cultivation of Euglena in EUGLENA: BIOCHEMISTRY, CELL AND MOLECULAR BIOLOGY
  • 2015-05. Alteration of Wax Ester Content and Composition in Euglena gracilis with Gene Silencing of 3-ketoacyl-CoA Thiolase Isozymes in LIPIDS
  • 2018-10. Current understanding of sulfur assimilation metabolism to biosynthesize l-cysteine and recent progress of its fermentative overproduction in microorganisms in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2016-12. De novo assembly and comparative transcriptome analysis of Euglena gracilis in response to anaerobic conditions in BMC GENOMICS
  • 1982-12. Dark-induced chloroplast dedifferentiation in Euglena gracilis in PLANTA
  • 2010-03. Fatty Acyl-CoA Reductase and Wax Synthase from Euglena gracilis in the Biosynthesis of Medium-Chain Wax Esters in LIPIDS
  • 1994-03. Metabolism of sulfate-reducing prokaryotes in ANTONIE VAN LEEUWENHOEK
  • 2015-12. Enhancement of photosynthetic capacity in Euglena gracilis by expression of cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase leads to increases in biomass and wax ester production in BIOTECHNOLOGY FOR BIOFUELS
  • 1948-10. Laboratory extraction of cottonseed with various petroleum hydrocarbons in JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY
  • 1999-11. Peptidases and amino acid catabolism in lactic acid bacteria in ANTONIE VAN LEEUWENHOEK
  • 2017. The Mitochondrion of Euglena gracilis in EUGLENA: BIOCHEMISTRY, CELL AND MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-36600-z

    DOI

    http://dx.doi.org/10.1038/s41598-018-36600-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1111768930

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30696857


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "RIKEN", 
              "id": "https://www.grid.ac/institutes/grid.7597.c", 
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan", 
                "Microalgae Production Control Technology Laboratory, RIKEN, 230-0045, Kanagawa, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yamada", 
            "givenName": "Koji", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nitta", 
            "givenName": "Tomoaki", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RIKEN", 
              "id": "https://www.grid.ac/institutes/grid.7597.c", 
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan", 
                "Microalgae Production Control Technology Laboratory, RIKEN, 230-0045, Kanagawa, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Atsuji", 
            "givenName": "Kohei", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Tsukuba", 
              "id": "https://www.grid.ac/institutes/grid.20515.33", 
              "name": [
                "Innovation Medical Research Institute, University of Tsukuba, 305-8577, Ibaraki, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shiroyama", 
            "givenName": "Maeka", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RIKEN", 
              "id": "https://www.grid.ac/institutes/grid.7597.c", 
              "name": [
                "Center for Sustainable Resource Science, RIKEN, 230-0045, Kanagawa, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Inoue", 
            "givenName": "Komaki", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Higuchi", 
            "givenName": "Chieko", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nitta", 
            "givenName": "Nobuko", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "National Institute Of Technology", 
              "id": "https://www.grid.ac/institutes/grid.482504.f", 
              "name": [
                "Innovation Medical Research Institute, University of Tsukuba, 305-8577, Ibaraki, Japan", 
                "Department of Bioresources Engineering, National Institute of Technology, Okinawa College, 905-2192, Okinawa, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Oshiro", 
            "givenName": "Satoshi", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Okayama University", 
              "id": "https://www.grid.ac/institutes/grid.261356.5", 
              "name": [
                "Microalgae Production Control Technology Laboratory, RIKEN, 230-0045, Kanagawa, Japan", 
                "Center for Sustainable Resource Science, RIKEN, 230-0045, Kanagawa, Japan", 
                "Kihara Institute for Biological Research, Yokohama City University, 244-0813, Kanagawa, Japan", 
                "Institute of Plant Science and Resources, Okayama University, 710-0046, Okayama, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mochida", 
            "givenName": "Keiichi", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RIKEN", 
              "id": "https://www.grid.ac/institutes/grid.7597.c", 
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan", 
                "Microalgae Production Control Technology Laboratory, RIKEN, 230-0045, Kanagawa, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Iwata", 
            "givenName": "Osamu", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Tsukuba", 
              "id": "https://www.grid.ac/institutes/grid.20515.33", 
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan", 
                "Innovation Medical Research Institute, University of Tsukuba, 305-8577, Ibaraki, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ohtsu", 
            "givenName": "Iwao", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "RIKEN", 
              "id": "https://www.grid.ac/institutes/grid.7597.c", 
              "name": [
                "Co., Ltd., 108-0014, Tokyo, Japan", 
                "Microalgae Production Control Technology Laboratory, RIKEN, 230-0045, Kanagawa, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Suzuki", 
            "givenName": "Kengo", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0014-5793(82)81310-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002182528"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1002001919720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003723200", 
              "https://doi.org/10.1023/a:1002001919720"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3389/fphys.2012.00448", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006848186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00410835", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012938325", 
              "https://doi.org/10.1007/bf00410835"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00410835", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012938325", 
              "https://doi.org/10.1007/bf00410835"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb1961.47.2669", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013237232"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02593281", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013269333", 
              "https://doi.org/10.1007/bf02593281"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.rser.2009.10.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014294971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj0980369", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014461507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj0980369", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014461507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/10643389609388489", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015975620"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11745-010-3395-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016481538", 
              "https://doi.org/10.1007/s11745-010-3395-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11745-010-3395-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016481538", 
              "https://doi.org/10.1007/s11745-010-3395-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.bi.50.070181.000323", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016528992"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0891-5849(01)00480-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018930552"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/mnfr.201500031", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019901322"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1515/bc.2009.033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022236606"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11745-015-4010-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022524521", 
              "https://doi.org/10.1007/s11745-015-4010-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/oxfordjournals.molbev.a003853", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024819616"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiosc.2014.08.012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027031883"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3389/fmicb.2016.02050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029104509"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.106.066944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031903528"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.106.066944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031903528"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1039/c0np00018c", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035137589"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btp324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038266369"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/09168451.2015.1045828", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038348292"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00871638", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039645373", 
              "https://doi.org/10.1007/bf00871638"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00871638", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039645373", 
              "https://doi.org/10.1007/bf00871638"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btu170", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042720804"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.57.3.430", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043880369"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.arplant.51.1.141", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044888280"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.biotechadv.2007.02.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045289972"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1152/japplphysiol.00449.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046864179"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00393736", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049087099", 
              "https://doi.org/10.1007/bf00393736"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00393736", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049087099", 
              "https://doi.org/10.1007/bf00393736"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1984.tb08258.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049114297"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s12864-016-2540-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050651980", 
              "https://doi.org/10.1186/s12864-016-2540-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep26327", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051990897", 
              "https://doi.org/10.1038/srep26327"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13068-015-0264-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052547286", 
              "https://doi.org/10.1186/s13068-015-0264-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13068-015-0264-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052547286", 
              "https://doi.org/10.1186/s13068-015-0264-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-319-54910-1_14", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084994673", 
              "https://doi.org/10.1007/978-3-319-54910-1_14"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-319-54910-1_2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085016779", 
              "https://doi.org/10.1007/978-3-319-54910-1_2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/s41598-016-0001-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085372625"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-018-9246-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1105830293", 
              "https://doi.org/10.1007/s00253-018-9246-4"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-12", 
        "datePublishedReg": "2019-12-01", 
        "description": "Euglena gracilis is a microalga, which has been used as a model organism for decades. Recent technological advances have enabled mass cultivation of this species for industrial applications such as feedstock in nutritional foods and cosmetics. E. gracilis degrades its storage polysaccharide (paramylon) under hypoxic conditions for energy acquisition by an oxygen-independent process and accumulates high amount of wax-ester as a by-product. Using this sequence of reactions referred to as wax-ester fermentation, E. gracilis is studied for its application in biofuel production. Although the wax-ester production pathway is well characterized, little is known regarding the biochemical reactions underlying the main metabolic route, especially, the existence of an unknown sulfur-compound metabolism implied by the nasty odor generation accompanying the wax-ester fermentation. In this study, we show sulfur-metabolomics of E. gracilis in aerobic and hypoxic conditions, to reveal the biochemical reactions that occur during wax-ester synthesis. Our results helped us in identifying hydrogen sulfide (H2S) as the nasty odor-producing component in wax-ester fermentation. In addition, the results indicate that glutathione and protein degrades during hypoxia, whereas cysteine, methionine, and their metabolites increase in the cells. This indicates that this shift of abundance in sulfur compounds is the cause of H2S synthesis.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/s41598-018-36600-z", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1045337", 
            "issn": [
              "2045-2322"
            ], 
            "name": "Scientific Reports", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "name": "Characterization of sulfur-compound metabolism underlying wax-ester fermentation in Euglena gracilis", 
        "pagination": "853", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "f617a6524c6c7243839b6959584b8f575bc5bfaa9133c07f24885ca49f440492"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30696857"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101563288"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/s41598-018-36600-z"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1111768930"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/s41598-018-36600-z", 
          "https://app.dimensions.ai/details/publication/pub.1111768930"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T08:59", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000327_0000000327/records_114985_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/s41598-018-36600-z"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36600-z'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36600-z'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36600-z'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/s41598-018-36600-z'


     

    This table displays all metadata directly associated to this object as RDF triples.

    279 TRIPLES      21 PREDICATES      66 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/s41598-018-36600-z schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author Nac31861cdf2d4c44851a540d4d3a2d99
    4 schema:citation sg:pub.10.1007/978-3-319-54910-1_14
    5 sg:pub.10.1007/978-3-319-54910-1_2
    6 sg:pub.10.1007/bf00393736
    7 sg:pub.10.1007/bf00410835
    8 sg:pub.10.1007/bf00871638
    9 sg:pub.10.1007/bf02593281
    10 sg:pub.10.1007/s00253-018-9246-4
    11 sg:pub.10.1007/s11745-010-3395-2
    12 sg:pub.10.1007/s11745-015-4010-3
    13 sg:pub.10.1023/a:1002001919720
    14 sg:pub.10.1038/srep26327
    15 sg:pub.10.1186/s12864-016-2540-6
    16 sg:pub.10.1186/s13068-015-0264-5
    17 https://doi.org/10.1002/mnfr.201500031
    18 https://doi.org/10.1016/0014-5793(82)81310-0
    19 https://doi.org/10.1016/j.biotechadv.2007.02.001
    20 https://doi.org/10.1016/j.jbiosc.2014.08.012
    21 https://doi.org/10.1016/j.rser.2009.10.009
    22 https://doi.org/10.1016/s0891-5849(01)00480-4
    23 https://doi.org/10.1038/s41598-016-0001-8
    24 https://doi.org/10.1039/c0np00018c
    25 https://doi.org/10.1042/bj0980369
    26 https://doi.org/10.1080/09168451.2015.1045828
    27 https://doi.org/10.1080/10643389609388489
    28 https://doi.org/10.1093/bioinformatics/btp324
    29 https://doi.org/10.1093/bioinformatics/btu170
    30 https://doi.org/10.1093/oxfordjournals.molbev.a003853
    31 https://doi.org/10.1104/pp.57.3.430
    32 https://doi.org/10.1111/j.1432-1033.1984.tb08258.x
    33 https://doi.org/10.1146/annurev.arplant.51.1.141
    34 https://doi.org/10.1146/annurev.bi.50.070181.000323
    35 https://doi.org/10.1152/japplphysiol.00449.2004
    36 https://doi.org/10.1271/bbb1961.47.2669
    37 https://doi.org/10.1515/bc.2009.033
    38 https://doi.org/10.1534/genetics.106.066944
    39 https://doi.org/10.3389/fmicb.2016.02050
    40 https://doi.org/10.3389/fphys.2012.00448
    41 schema:datePublished 2019-12
    42 schema:datePublishedReg 2019-12-01
    43 schema:description Euglena gracilis is a microalga, which has been used as a model organism for decades. Recent technological advances have enabled mass cultivation of this species for industrial applications such as feedstock in nutritional foods and cosmetics. E. gracilis degrades its storage polysaccharide (paramylon) under hypoxic conditions for energy acquisition by an oxygen-independent process and accumulates high amount of wax-ester as a by-product. Using this sequence of reactions referred to as wax-ester fermentation, E. gracilis is studied for its application in biofuel production. Although the wax-ester production pathway is well characterized, little is known regarding the biochemical reactions underlying the main metabolic route, especially, the existence of an unknown sulfur-compound metabolism implied by the nasty odor generation accompanying the wax-ester fermentation. In this study, we show sulfur-metabolomics of E. gracilis in aerobic and hypoxic conditions, to reveal the biochemical reactions that occur during wax-ester synthesis. Our results helped us in identifying hydrogen sulfide (H<sub>2</sub>S) as the nasty odor-producing component in wax-ester fermentation. In addition, the results indicate that glutathione and protein degrades during hypoxia, whereas cysteine, methionine, and their metabolites increase in the cells. This indicates that this shift of abundance in sulfur compounds is the cause of H<sub>2</sub>S synthesis.
    44 schema:genre research_article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree true
    47 schema:isPartOf Nc2ef5d345ea54f05bedd1e7f4b78fbdd
    48 Nfa062fa71afe4b988a5898082cbde28d
    49 sg:journal.1045337
    50 schema:name Characterization of sulfur-compound metabolism underlying wax-ester fermentation in Euglena gracilis
    51 schema:pagination 853
    52 schema:productId N4051b379e47941e4916e6a55ad0b8887
    53 N62989f704c7c43f6b11188927d5e149a
    54 N89c54d354565455695dedcbf0d122611
    55 Nc532dfd52966406e8092341314e8c93b
    56 Nc56809bd15cb48dbb17386607b4a0e4f
    57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1111768930
    58 https://doi.org/10.1038/s41598-018-36600-z
    59 schema:sdDatePublished 2019-04-11T08:59
    60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    61 schema:sdPublisher N30ad20e423bf44e6bd727bc016bc7954
    62 schema:url https://www.nature.com/articles/s41598-018-36600-z
    63 sgo:license sg:explorer/license/
    64 sgo:sdDataset articles
    65 rdf:type schema:ScholarlyArticle
    66 N011a1e7aaafd4eecb879d39e0337e0f2 rdf:first N3ecff59c68134d6c903db1ef6cfe035d
    67 rdf:rest N3a7ea755d2884c8da7af253dbbc2bd5e
    68 N01dcfe8dc2904fd88f7b7102d99c8fd5 schema:affiliation N489d47219d2b4343840d13c7636c30ba
    69 schema:familyName Higuchi
    70 schema:givenName Chieko
    71 rdf:type schema:Person
    72 N05670c048f06434abb3212da499e4dbe rdf:first Ne707ec2397a4439d9dddd8c9422d2466
    73 rdf:rest N0ff9c8b221e04b418f7e04df560282fd
    74 N0f237b96dc6741b8ab41ad904e18a97c schema:affiliation https://www.grid.ac/institutes/grid.261356.5
    75 schema:familyName Mochida
    76 schema:givenName Keiichi
    77 rdf:type schema:Person
    78 N0ff9c8b221e04b418f7e04df560282fd rdf:first N337371bcc4ca4cdf991a78adee26096f
    79 rdf:rest rdf:nil
    80 N2486252289ad4750af99fba3cd396c21 rdf:first N01dcfe8dc2904fd88f7b7102d99c8fd5
    81 rdf:rest Nfeed1ce809cd4721b0101be98bb7255e
    82 N24c26259ab2f405fa8d0359df8113eb9 schema:affiliation https://www.grid.ac/institutes/grid.20515.33
    83 schema:familyName Shiroyama
    84 schema:givenName Maeka
    85 rdf:type schema:Person
    86 N24dcea50047d450196519f27f440c996 schema:name Co., Ltd., 108-0014, Tokyo, Japan
    87 rdf:type schema:Organization
    88 N2981eeb40b924b48aa0b4754c55697d0 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
    89 schema:familyName Iwata
    90 schema:givenName Osamu
    91 rdf:type schema:Person
    92 N2e757809fb2c4c2e9abab232f39715ae schema:name Co., Ltd., 108-0014, Tokyo, Japan
    93 rdf:type schema:Organization
    94 N30ad20e423bf44e6bd727bc016bc7954 schema:name Springer Nature - SN SciGraph project
    95 rdf:type schema:Organization
    96 N337371bcc4ca4cdf991a78adee26096f schema:affiliation https://www.grid.ac/institutes/grid.7597.c
    97 schema:familyName Suzuki
    98 schema:givenName Kengo
    99 rdf:type schema:Person
    100 N3a7ea755d2884c8da7af253dbbc2bd5e rdf:first Nf98685c092b84ff3bc87d439be5ca0b1
    101 rdf:rest Neeaac535ce4f41408d379cbe9f1d22be
    102 N3ecff59c68134d6c903db1ef6cfe035d schema:affiliation N2e757809fb2c4c2e9abab232f39715ae
    103 schema:familyName Nitta
    104 schema:givenName Tomoaki
    105 rdf:type schema:Person
    106 N4051b379e47941e4916e6a55ad0b8887 schema:name dimensions_id
    107 schema:value pub.1111768930
    108 rdf:type schema:PropertyValue
    109 N489d47219d2b4343840d13c7636c30ba schema:name Co., Ltd., 108-0014, Tokyo, Japan
    110 rdf:type schema:Organization
    111 N6129cb9f510b41c6920e441a3a3ebb69 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
    112 schema:familyName Yamada
    113 schema:givenName Koji
    114 rdf:type schema:Person
    115 N62989f704c7c43f6b11188927d5e149a schema:name nlm_unique_id
    116 schema:value 101563288
    117 rdf:type schema:PropertyValue
    118 N67d8ef4ab57445ecb66107d12555ff6b schema:affiliation https://www.grid.ac/institutes/grid.7597.c
    119 schema:familyName Inoue
    120 schema:givenName Komaki
    121 rdf:type schema:Person
    122 N8042bfb6c12f4fb883a296cace996c59 rdf:first N0f237b96dc6741b8ab41ad904e18a97c
    123 rdf:rest N888dfdcc6c9a438c872bb21c5975bc73
    124 N888dfdcc6c9a438c872bb21c5975bc73 rdf:first N2981eeb40b924b48aa0b4754c55697d0
    125 rdf:rest N05670c048f06434abb3212da499e4dbe
    126 N89c54d354565455695dedcbf0d122611 schema:name doi
    127 schema:value 10.1038/s41598-018-36600-z
    128 rdf:type schema:PropertyValue
    129 Na8136a29c13d4a42a4659d14b5eb9360 schema:affiliation N24dcea50047d450196519f27f440c996
    130 schema:familyName Nitta
    131 schema:givenName Nobuko
    132 rdf:type schema:Person
    133 Nac31861cdf2d4c44851a540d4d3a2d99 rdf:first N6129cb9f510b41c6920e441a3a3ebb69
    134 rdf:rest N011a1e7aaafd4eecb879d39e0337e0f2
    135 Nc2ef5d345ea54f05bedd1e7f4b78fbdd schema:issueNumber 1
    136 rdf:type schema:PublicationIssue
    137 Nc532dfd52966406e8092341314e8c93b schema:name pubmed_id
    138 schema:value 30696857
    139 rdf:type schema:PropertyValue
    140 Nc56809bd15cb48dbb17386607b4a0e4f schema:name readcube_id
    141 schema:value f617a6524c6c7243839b6959584b8f575bc5bfaa9133c07f24885ca49f440492
    142 rdf:type schema:PropertyValue
    143 Ndff733f1e7bd475c96a71d0fb466fd77 schema:affiliation https://www.grid.ac/institutes/grid.482504.f
    144 schema:familyName Oshiro
    145 schema:givenName Satoshi
    146 rdf:type schema:Person
    147 Ne707ec2397a4439d9dddd8c9422d2466 schema:affiliation https://www.grid.ac/institutes/grid.20515.33
    148 schema:familyName Ohtsu
    149 schema:givenName Iwao
    150 rdf:type schema:Person
    151 Nebfe9ed514ca448bb71eeac30d49971b rdf:first N67d8ef4ab57445ecb66107d12555ff6b
    152 rdf:rest N2486252289ad4750af99fba3cd396c21
    153 Neeaac535ce4f41408d379cbe9f1d22be rdf:first N24c26259ab2f405fa8d0359df8113eb9
    154 rdf:rest Nebfe9ed514ca448bb71eeac30d49971b
    155 Nf98685c092b84ff3bc87d439be5ca0b1 schema:affiliation https://www.grid.ac/institutes/grid.7597.c
    156 schema:familyName Atsuji
    157 schema:givenName Kohei
    158 rdf:type schema:Person
    159 Nfa062fa71afe4b988a5898082cbde28d schema:volumeNumber 9
    160 rdf:type schema:PublicationVolume
    161 Nfdfc83448cf841d9a147cd55b6a395fa rdf:first Ndff733f1e7bd475c96a71d0fb466fd77
    162 rdf:rest N8042bfb6c12f4fb883a296cace996c59
    163 Nfeed1ce809cd4721b0101be98bb7255e rdf:first Na8136a29c13d4a42a4659d14b5eb9360
    164 rdf:rest Nfdfc83448cf841d9a147cd55b6a395fa
    165 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    166 schema:name Biological Sciences
    167 rdf:type schema:DefinedTerm
    168 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    169 schema:name Biochemistry and Cell Biology
    170 rdf:type schema:DefinedTerm
    171 sg:journal.1045337 schema:issn 2045-2322
    172 schema:name Scientific Reports
    173 rdf:type schema:Periodical
    174 sg:pub.10.1007/978-3-319-54910-1_14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084994673
    175 https://doi.org/10.1007/978-3-319-54910-1_14
    176 rdf:type schema:CreativeWork
    177 sg:pub.10.1007/978-3-319-54910-1_2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085016779
    178 https://doi.org/10.1007/978-3-319-54910-1_2
    179 rdf:type schema:CreativeWork
    180 sg:pub.10.1007/bf00393736 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049087099
    181 https://doi.org/10.1007/bf00393736
    182 rdf:type schema:CreativeWork
    183 sg:pub.10.1007/bf00410835 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012938325
    184 https://doi.org/10.1007/bf00410835
    185 rdf:type schema:CreativeWork
    186 sg:pub.10.1007/bf00871638 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039645373
    187 https://doi.org/10.1007/bf00871638
    188 rdf:type schema:CreativeWork
    189 sg:pub.10.1007/bf02593281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013269333
    190 https://doi.org/10.1007/bf02593281
    191 rdf:type schema:CreativeWork
    192 sg:pub.10.1007/s00253-018-9246-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105830293
    193 https://doi.org/10.1007/s00253-018-9246-4
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1007/s11745-010-3395-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016481538
    196 https://doi.org/10.1007/s11745-010-3395-2
    197 rdf:type schema:CreativeWork
    198 sg:pub.10.1007/s11745-015-4010-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022524521
    199 https://doi.org/10.1007/s11745-015-4010-3
    200 rdf:type schema:CreativeWork
    201 sg:pub.10.1023/a:1002001919720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003723200
    202 https://doi.org/10.1023/a:1002001919720
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1038/srep26327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051990897
    205 https://doi.org/10.1038/srep26327
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1186/s12864-016-2540-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050651980
    208 https://doi.org/10.1186/s12864-016-2540-6
    209 rdf:type schema:CreativeWork
    210 sg:pub.10.1186/s13068-015-0264-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052547286
    211 https://doi.org/10.1186/s13068-015-0264-5
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1002/mnfr.201500031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019901322
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1016/0014-5793(82)81310-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002182528
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1016/j.biotechadv.2007.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045289972
    218 rdf:type schema:CreativeWork
    219 https://doi.org/10.1016/j.jbiosc.2014.08.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027031883
    220 rdf:type schema:CreativeWork
    221 https://doi.org/10.1016/j.rser.2009.10.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014294971
    222 rdf:type schema:CreativeWork
    223 https://doi.org/10.1016/s0891-5849(01)00480-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018930552
    224 rdf:type schema:CreativeWork
    225 https://doi.org/10.1038/s41598-016-0001-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085372625
    226 rdf:type schema:CreativeWork
    227 https://doi.org/10.1039/c0np00018c schema:sameAs https://app.dimensions.ai/details/publication/pub.1035137589
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1042/bj0980369 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014461507
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1080/09168451.2015.1045828 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038348292
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1080/10643389609388489 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015975620
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1093/bioinformatics/btp324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038266369
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1093/bioinformatics/btu170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042720804
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1093/oxfordjournals.molbev.a003853 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024819616
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1104/pp.57.3.430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043880369
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1111/j.1432-1033.1984.tb08258.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049114297
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1146/annurev.arplant.51.1.141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044888280
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1146/annurev.bi.50.070181.000323 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016528992
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1152/japplphysiol.00449.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046864179
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1271/bbb1961.47.2669 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013237232
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1515/bc.2009.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022236606
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1534/genetics.106.066944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031903528
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.3389/fmicb.2016.02050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029104509
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.3389/fphys.2012.00448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006848186
    260 rdf:type schema:CreativeWork
    261 https://www.grid.ac/institutes/grid.20515.33 schema:alternateName University of Tsukuba
    262 schema:name Co., Ltd., 108-0014, Tokyo, Japan
    263 Innovation Medical Research Institute, University of Tsukuba, 305-8577, Ibaraki, Japan
    264 rdf:type schema:Organization
    265 https://www.grid.ac/institutes/grid.261356.5 schema:alternateName Okayama University
    266 schema:name Center for Sustainable Resource Science, RIKEN, 230-0045, Kanagawa, Japan
    267 Institute of Plant Science and Resources, Okayama University, 710-0046, Okayama, Japan
    268 Kihara Institute for Biological Research, Yokohama City University, 244-0813, Kanagawa, Japan
    269 Microalgae Production Control Technology Laboratory, RIKEN, 230-0045, Kanagawa, Japan
    270 rdf:type schema:Organization
    271 https://www.grid.ac/institutes/grid.482504.f schema:alternateName National Institute Of Technology
    272 schema:name Department of Bioresources Engineering, National Institute of Technology, Okinawa College, 905-2192, Okinawa, Japan
    273 Innovation Medical Research Institute, University of Tsukuba, 305-8577, Ibaraki, Japan
    274 rdf:type schema:Organization
    275 https://www.grid.ac/institutes/grid.7597.c schema:alternateName RIKEN
    276 schema:name Center for Sustainable Resource Science, RIKEN, 230-0045, Kanagawa, Japan
    277 Co., Ltd., 108-0014, Tokyo, Japan
    278 Microalgae Production Control Technology Laboratory, RIKEN, 230-0045, Kanagawa, Japan
    279 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...