Targeting CD40 enhances antibody- and CD8-mediated protection against respiratory syncytial virus infection View Full Text


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Article Info

DATE

2018-11-09

AUTHORS

Abenaya Muralidharan, Marsha Russell, Louise Larocque, Caroline Gravel, Changgui Li, Wangxue Chen, Terry Cyr, Jessie R. Lavoie, Aaron Farnsworth, Michael Rosu-Myles, Lisheng Wang, Xuguang Li

ABSTRACT

Respiratory Syncytial Virus (RSV) infects almost all children under the age of one and is the leading cause of hospitalization among infants. Despite several decades of research with dozens of candidate vaccines being vigorously evaluated in pre-clinical and clinical studies, there is no licensed vaccine available to date. Here, the RSV fusion protein (F) was fused with CD40 ligand and delivered by an adenoviral vector into BALB/c mice where the CD40 ligand serves two vital functions as a molecular adjuvant and an antigen-targeting molecule. In contrast to a formaldehyde-inactivated vaccine, the vectored vaccine effectively protected animals against RSV without inducing enhanced respiratory disease. This protection involved a robust induction of neutralizing antibodies and memory CD8 T cells, which were not observed in the inactivated vaccine group. Finally, the vectored vaccine was able to elicit long-lasting protection against RSV, one of the most challenging issues in RSV vaccine development. Further studies indicate that the long lasting protection elicited by the CD40 ligand targeted vaccine was mediated by increased levels of effector memory CD8 T cell 3 months post-vaccination. More... »

PAGES

16648

References to SciGraph publications

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/s41598-018-34999-z

    DOI

    http://dx.doi.org/10.1038/s41598-018-34999-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1108043177

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30413743


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